RESUMO
Phytochemical investigation of ethanol extract from the flowers of Trollius chinensis Bunge resulted in the isolation of two new flavone C-glycosides (1-2), along with 10 known compounds (3-12). The structures of the new compounds were established as 6â´-(3-hydroxy-3-methylglutaroyl)-2â³-O-ß-d-galactopyranosyl orientin (1) and 6â´-(3-hydroxy-3-methylglutaroyl)-2â³-O-ß-d-galactopyranosyl vitexin (2) on the basis of various spectroscopic analysis (including different 1D and 2D NMR spectroscopies, high-resolution electrospray ionization mass spectrometry) and chemical evidences. Bioassay showed that eight flavonoids inhibited complement activation on the classic pathway in vitro, with their IC50 values ranging from 0.88 to 4.02 mM, which may contribute to the applications of the herb in treatment of acute respiratory distress syndrome, etc.
Assuntos
Inativadores do Complemento/isolamento & purificação , Inativadores do Complemento/farmacologia , Medicamentos de Ervas Chinesas/isolamento & purificação , Medicamentos de Ervas Chinesas/farmacologia , Flavonas/isolamento & purificação , Flavonas/farmacologia , Glicosídeos/isolamento & purificação , Glicosídeos/farmacologia , Ranunculaceae/química , Inativadores do Complemento/química , Medicamentos de Ervas Chinesas/química , Flavonas/química , Flores/química , Glicosídeos/química , Estrutura Molecular , Ressonância Magnética Nuclear BiomolecularRESUMO
A new sulphonoglycolipid, crassicaulisine, has been isolated from the red alga Chondria crassicaulis Harv.. Four known compounds were also found from the title plant. The structure of the new compound was elucidated on the basis of chemical reactions and spectroscopic analysis.