RESUMO
A new isolated dibenzothiophene (DBT) desulfurizing bacterium, identified as Mycobacterium sp. ZD-19 can utilize a wide range of organic sulfur compounds as a sole sulfur source. Thiophene (TH) or benzothiophene (BTH) was completely degraded by strain ZD-19 within 10h or 42 h, and 100% DBT or 4,6-dimethyldibenzothiophene (4,6-DMDBT) was removed within 50h or 56 h, respectively. Diphenylsulfide (DPS) possessed the lowest desulfurization efficiencies with 60% being transformed within 50h and 80% at 90 h. The desulfurization activities of five substrates by resting cells are in order of TH>BTH>DPS>DBT>4,6-DMDBT. In addition, when DBT and 4,6-DMDBT were mixed, they could be simultaneously desulfurized by strain ZD-19. However, DBT appeared to be attacked prior to 4,6-DMDBT. The desulfurization rate of DBT or 4,6-DMDBT in mixture is lower than they are desulfurized separately, indicating that the substrate competitive inhibition is existent when DBT and 4,6-DMDBT are mixed.
Assuntos
Mycobacterium/isolamento & purificação , Compostos de Enxofre/metabolismo , Enxofre/isolamento & purificação , Tiofenos/isolamento & purificação , Biodegradação Ambiental , Mycobacterium/genética , Mycobacterium/crescimento & desenvolvimento , Filogenia , RNA Ribossômico 16S/genética , Fatores de TempoRESUMO
The effect of 2-hydroxybiphenyl (2-HBP), the end product of dibenzothiophene (DBT) desulfurization via 4S pathway, on cell growth and desulfurization activity was investigated by Microbacterium sp. The experimental results indicate that 2-HBP would inhibit the desulfurization activity. Providing 2-HBP was added in the reaction media, the DBT degradation rate decreased along with the increase of 2-HBP addition. By contrast, cell growth would be promoted in the addition of 2-HBP at a low concentration (<0.1mM). At high concentration of 2-HBP, the inhibition on the cell growth occurred. Meanwhile, the inhibitory effect of 2-HBP on DBT desulfurization activity was tested both in the oil/aqueous two-phase system and the aqueous system. A mathematical model was developed to explain the product formation kinetics with DBT as the sole sulfur source. The predicted results were close to the experimental data, it elucidated that along with the 2-HBP accumulation, the inhibitory effect of 2-HBP on DBT desulfurization and cell growth was enhanced.
Assuntos
Compostos de Bifenilo/metabolismo , Bactérias Gram-Positivas/metabolismo , Enxofre/metabolismo , Tiofenos/metabolismo , Meios de Cultura , CinéticaRESUMO
A metabolic pathway for the biodesulfurization of model organosulfur compounds e.g., dibenzothiophene (DBT), is proposed. This pathway, defined as extended 4S pathway, incorporates the traditional 4S pathway with the methoxylation pathway from 2-hydroxybiphenyl (HBP) to 2-methoxybiphenyl (2-MBP). The formation of 2-MBP was confirmed by the gas chromatography-mass spectrometry (GC-MS) analysis. A similar pathway was also obtained in the desulfurization of 4,6-dimethyldibenzothiophene (4,6-DMDBT), confirming the methoxylation reaction in the desulfurization process by the Mycobacterium sp. strain. Compared with 2-HBP, 2-MBP has much slighter inhibition effect on the cell growth and desulfurization activity. Thus, the methoxylation pathway from 2-HBP to 2-MBP would make less inhibitory effect on the microbe. The new pathway with 2-MBP as the end product may be an alternative for the further desulfuration of the fossil fuels.