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Am J Physiol Cell Physiol ; 317(4): C719-C724, 2019 10 01.
Artigo em Inglês | MEDLINE | ID: mdl-31314585

RESUMO

It is postulated that testosterone-induced skeletal muscle hypertrophy is driven by myonuclear accretion as the result of satellite cell fusion. To directly test this hypothesis, we utilized the Pax7-DTA mouse model to deplete satellite cells in skeletal muscle followed by testosterone administration. Pax7-DTA mice (6 mo of age) were treated for 5 days with either vehicle [satellite cell replete (SC+)] or tamoxifen [satellite cell depleted (SC-)]. Following a washout period, a testosterone propionate or sham pellet was implanted for 21 days. Testosterone administration caused a significant increase in muscle fiber cross-sectional area in SC+ and SC- mice in both oxidative (soleus) and glycolytic (plantaris and extensor digitorum longus) muscles. In SC+ mice treated with testosterone, there was a significant increase in both satellite cell abundance and myonuclei that was completely absent in testosterone-treated SC- mice. These findings provide direct evidence that testosterone-induced muscle fiber hypertrophy does not require an increase in satellite cell abundance or myonuclear accretion.Listen to a podcast about this Rapid Report with senior author E. E. Dupont-Versteegden (https://ajpcell.podbean.com/e/podcast-on-paper-that-shows-testosterone-induced-skeletal-muscle-hypertrophy-does-not-need-muscle-stem-cells/).


Assuntos
Fibras Musculares Esqueléticas/efeitos dos fármacos , Células Satélites de Músculo Esquelético/efeitos dos fármacos , Células-Tronco/efeitos dos fármacos , Testosterona/farmacologia , Animais , Modelos Animais de Doenças , Hipertrofia/induzido quimicamente , Camundongos Transgênicos , Fibras Musculares Esqueléticas/fisiologia , Fator de Transcrição PAX7/genética , Células Satélites de Músculo Esquelético/fisiologia
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