1.
Appl Environ Microbiol
; 75(24): 7870-2, 2009 Dec.
Artigo
em Inglês
| MEDLINE
| ID: mdl-19837835
RESUMO
A microtiter alamarBlue assay was adapted and optimized for Mycobacterium avium subsp. paratuberculosis. Using cell concentrations ranging from 10(4) to 10(8) CFU/ml, a minimum incubation time to indicate viability was obtained after 24 h. Rifampin (rifampicin) was used to demonstrate that this method has applications for high-throughput screening against M. avium subsp. paratuberculosis.