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1.
Epidemiol Infect ; 142(12): 2604-9, 2014 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-24534556

RESUMO

Epidemiological and virological studies indicate that noroviruses-contaminated groundwater was the primary source of four acute gastroenteritis outbreaks in South Korea between 2008 and 2012. Furthermore, cabbage kimchi was first identified as the vehicle of transmission between groundwater and infected patients in an outbreak in 2011. The proper treatment of groundwater sources prior to use for drinking or in food preparation is necessary to prevent further outbreaks.


Assuntos
Brassica , Infecções por Caliciviridae/epidemiologia , Infecções por Caliciviridae/virologia , Surtos de Doenças , Gastroenterite/epidemiologia , Gastroenterite/virologia , Norovirus/isolamento & purificação , Microbiologia da Água , Doença Aguda , Feminino , Humanos , Masculino , Dados de Sequência Molecular , Norovirus/genética , Filogenia , República da Coreia/epidemiologia
2.
Clin Lab ; 57(11-12): 959-67, 2011.
Artigo em Inglês | MEDLINE | ID: mdl-22239028

RESUMO

BACKGROUND: Human enteroviruses (HEVs) are a major cause of herpangina, HFMD (hand, foot, and mouth disease), and other neurological diseases in Seoul, Korea. METHODS: A total of 56 specimens from hospitalized patients collected from February to December 2009 (37 females and 19 males) in Seoul were tested for HEV from stool, throat swab, and vesicle swab samples taken from patients with herpangina or HFMD using cell culture and RT-PCR in 2009. By the 1D gene, encoding the VP1 capsid protein, seven different HEV genotypes were detected with Coxsackievirus A2, A4, A5, A9, A16 (CA), Coxsackievirus B1 (CB), and Enterovirus 71 (EV71). The most prevalent genotype was CA16 (6, 10.7%), followed by CA2 (4, 7.1%), CA5 (4, 7.1%), EV71 (2, 3.6%), CA4 (1, 1.8%), CA9 (1, 1.8%), and CB1 (1, 1.8%). The 1D gene sequences of two EV71 strains were closely related with one another (98.5% nucleotide similarity) and belonged to the C4 genotype. CONCLUSIONS: It is important to continuously survey the genetic characteristics of EV71 and CA16 from patients, which will provide useful data that aids in our understanding of HFMD infections in Seoul, Korea and may contribute to future control.


Assuntos
Infecções por Coxsackievirus/virologia , Surtos de Doenças , Infecções por Enterovirus/virologia , Enterovirus/isolamento & purificação , Doença de Mão, Pé e Boca/virologia , Herpangina/virologia , Proteínas do Capsídeo/genética , Pré-Escolar , Infecções por Coxsackievirus/epidemiologia , Enterovirus/genética , Enterovirus Humano A/genética , Enterovirus Humano A/isolamento & purificação , Enterovirus Humano B/genética , Enterovirus Humano B/isolamento & purificação , Infecções por Enterovirus/epidemiologia , Fezes/virologia , Feminino , Doença de Mão, Pé e Boca/epidemiologia , Herpangina/epidemiologia , Humanos , Lactente , Recém-Nascido , Masculino , Faringe/virologia , Filogenia , RNA Viral/genética , RNA Viral/isolamento & purificação , República da Coreia/epidemiologia , Análise de Sequência de RNA
3.
Arch Virol ; 155(5): 635-41, 2010 May.
Artigo em Inglês | MEDLINE | ID: mdl-20224892

RESUMO

Noroviruses are the enteric pathogens most commonly responsible for infectious gastroenteritis and outbreaks of foodborne illness. The GII.4 norovirus, in particular, is responsible for the majority of epidemics. Here, we present data on the distribution of norovirus genotypes in Chungnam, Korea, in 2008, measure genetic variation among GII.4 strains, and compare Korean GII.4 variants with reference strains based on the 237-bp junction of ORF1 and ORF2. We detected 139 different strains, which formed two distinct genetic clusters with significant sequence diversity. One Korean cluster (2008-Korea_a) showed high similarity to the Sakai cluster that appeared in Japan and Europe in 2006. The other cluster (2008-Korea_b) was unique and unrelated to previously reported clusters. Genotype GII.4 was confirmed as the predominant cause of norovirus epidemics in Korea. Foodborne norovirus infections, on the other hand, were generally caused by emerging GII.4 genetic variants similar to those responsible for global epidemics.


Assuntos
Gastroenterite/virologia , Norovirus/genética , Adulto , Idoso , Infecções por Caliciviridae/virologia , Criança , Variação Genética , Humanos , Coreia (Geográfico) , Pessoa de Meia-Idade , Norovirus/classificação , Filogenia , Fatores de Tempo
4.
J Vet Diagn Invest ; 12(6): 582-7, 2000 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-11108465

RESUMO

Virus isolation, reverse transcription-polymerase chain reaction (RT-PCR), immunohistochemistry, and in situ hybridization methods were compared for the detection of porcine reproductive and respiratory syndrome virus (PRRSV). Seven aborted fetuses and 6 stillborn piglets naturally infected with PRRSV were used in the study. Viral antigen and viral nucleic acid were detected in macrophages and dendritic cells in the spleen, tonsil, lymph nodes, and thymus; in macrophages of liver, heart, and lung; and in endothelial cells and myocytes of the heart. Viral antigen and viral nucleic acid were most consistently detected in the spleen. Of the 13 samples, 6 were positive for PRRSV by all 4 techniques. Four (31%) samples were positive for PRRSV by RT-PCR, in situ hybridization, and virus isolation. Two (15%) samples were positive for PRRSV by virus isolation, RT-PCR, and in situ hybridization. One (8%) was positive for PRRSV by virus isolation and RT-PCR. The RT-PCR identified the presence of PRRSV more frequently than the other methods. However, when only formalin-fixed tissues are submitted, immunohistochemistry and in situ hybridization would be useful methods for the detection of PRRSV antigen and nucleic acid.


Assuntos
Aborto Animal/virologia , Morte Fetal/veterinária , Síndrome Respiratória e Reprodutiva Suína/patologia , Vírus da Síndrome Respiratória e Reprodutiva Suína/isolamento & purificação , Aborto Animal/patologia , Animais , Feminino , Morte Fetal/patologia , Morte Fetal/virologia , Imuno-Histoquímica/métodos , Hibridização In Situ/métodos , Macrófagos/patologia , Macrófagos/virologia , Reação em Cadeia da Polimerase/métodos , Síndrome Respiratória e Reprodutiva Suína/embriologia , Gravidez , Reprodutibilidade dos Testes , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Suínos
5.
J Comp Pathol ; 124(4): 231-7, 2001 May.
Artigo em Inglês | MEDLINE | ID: mdl-11437498

RESUMO

Four pregnant sows were infected 3 weeks before their expected farrowing date with a Korean isolate (North American genotype) of porcine reproductive and respiratory syndrome virus (PRRSV). The distribution of virus in their stillborn and liveborn (killed 7 days after birth) offspring was assessed immunohistochemically and by in-situ hybridization. PRRSV antigen and nucleic acid were detected in lung, thymus, liver, tonsil, spleen, heart, kidney and lymph nodes from both stillborn and liveborn piglets. Positive cells typically exhibited a red (immunohistochemistry) or dark brown (in-situ hybridization) reaction product in the cytoplasm, without background staining. The most consistent labelling for PRRSV was in the thymus, tonsil and lymph nodes. The experiment suggested that, in prenatal piglets, PRRSV replicates primarily in lymphoid tissues, having gained access to them from the placenta via the bloodstream.


Assuntos
Animais Recém-Nascidos/virologia , Morte Fetal/virologia , Síndrome Respiratória e Reprodutiva Suína/virologia , Vírus da Síndrome Respiratória e Reprodutiva Suína/isolamento & purificação , Suínos , Animais , Modelos Animais de Doenças , Feminino , Imuno-Histoquímica/veterinária , Hibridização In Situ/veterinária , Síndrome Respiratória e Reprodutiva Suína/patologia , Síndrome Respiratória e Reprodutiva Suína/transmissão , Vírus da Síndrome Respiratória e Reprodutiva Suína/genética , Vírus da Síndrome Respiratória e Reprodutiva Suína/crescimento & desenvolvimento , Vírus da Síndrome Respiratória e Reprodutiva Suína/imunologia , Gravidez , Replicação Viral
6.
J Comp Pathol ; 130(2-3): 105-11, 2004.
Artigo em Inglês | MEDLINE | ID: mdl-15003466

RESUMO

The objective of this study was to compare two Korean strains of porcine reproductive and respiratory syndrome virus (PRRSV), namely a wild type (WT) strain and a vaccine-like (VL) strain, in respect of pathogenicity and viral distribution in the tissues. Both strains were of the North American genotype. Two groups of five pregnant sows were infected with either the WT or the VL strain 2 weeks before their expected farrowing date. The WT strain-inoculated sows showed abortion and premature farrowing, whereas the VL strain-inoculated sows remained clinically normal and did not farrow prematurely. Of the 18 liveborn piglets from the WT strain-inoculated sows, 14 had interstitial pneumonia. Of the 60 liveborn piglets from the VL strain-inoculated sows, only six had interstitial pneumonia. PRRSV antigen or nucleic acid was detected in 48/65 (73.8%) of stillborn and liveborn piglets from the WT strain-inoculated sows, but in only 12/64 (18.8%) of stillborn and liveborn piglets from the VL strain-inoculated sows.


Assuntos
Síndrome Respiratória e Reprodutiva Suína/patologia , Vírus da Síndrome Respiratória e Reprodutiva Suína/genética , Vírus da Síndrome Respiratória e Reprodutiva Suína/patogenicidade , Complicações Infecciosas na Gravidez/veterinária , Doenças dos Suínos/virologia , Animais , Anticorpos Antivirais/sangue , Feminino , Coração/virologia , Imuno-Histoquímica , Hibridização In Situ , Transmissão Vertical de Doenças Infecciosas/veterinária , Pulmão/patologia , Pulmão/virologia , Miocárdio/patologia , Polimorfismo de Fragmento de Restrição , Síndrome Respiratória e Reprodutiva Suína/transmissão , Gravidez , Complicações Infecciosas na Gravidez/virologia , Resultado da Gravidez/veterinária , Baço/patologia , Baço/virologia , Suínos
7.
J Comp Pathol ; 120(1): 79-88, 1999 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-10098017

RESUMO

In an experiment with 40 specific pathogen-free pigs aged 3 days, the distribution of a Korean isolate of porcine reproductive and respiratory syndrome virus (PRRSV) was assessed immunohistochemically and by in-situ hybridization for a period of 28 days after intranasal inoculation. The most consistent and intense labelling for PRRSV was in the lung, the virus persisting in pulmonary macrophages for at least 28 days. The middle lobe of the lung was the optimum site for the detection of PRRSV antigens and nucleic acids, and the interstitial macrophage was the main cell type in which PRRSV was identified. Other tissues and cells in which the virus was detected included macrophages and dendritic cells in the tonsil, lymph nodes, spleen and Peyer's patches, and macrophages in the hepatic sinusoids and adrenal gland. The experiment suggested that the pathogenesis of PRRSV infection may be summarized thus: initial entry of virus through tonsillar and pulmonary macrophages, followed within 3 days by viraemia and subsequent interstitial pneumonia.


Assuntos
Síndrome Respiratória e Reprodutiva Suína/virologia , Vírus da Síndrome Respiratória e Reprodutiva Suína/isolamento & purificação , Animais , Antígenos Virais/análise , Células Dendríticas/virologia , Imuno-Histoquímica , Hibridização In Situ/veterinária , Pulmão/patologia , Pulmão/virologia , Linfonodos/virologia , Macrófagos Alveolares/virologia , Tonsila Palatina/virologia , Síndrome Respiratória e Reprodutiva Suína/patologia , Vírus da Síndrome Respiratória e Reprodutiva Suína/genética , Vírus da Síndrome Respiratória e Reprodutiva Suína/imunologia , RNA Viral/análise , Organismos Livres de Patógenos Específicos , Baço/virologia , Suínos , Fatores de Tempo
8.
J Comp Pathol ; 117(2): 157-63, 1997 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-9352440

RESUMO

Replication of porcine reproductive and respiratory syndrome virus (PRRSV) was studied in formalin-fixed paraffin wax-embedded lung tissues from seven naturally infected piglets by in-situ hybridization with a non-radioactive digoxigenin-labelled probe. A 433 base pair cDNA probe for the viral RNA encoding the nucleocapsid proteins of a Korean PRRSV isolate was generated by the polymerase chain reaction. All seven piglets infected with PRRSV showed a distinct, positive signal, scattered throughout the alveolar septa and spaces. Positive cells typically exhibited dark brown staining deposits in the cytoplasm without background staining. In-situ hybridization demonstrated that PRRSV replicated primarily in interstitial and alveolar macrophages, and occasionally in type 2 pneumocytes. The bronchial or bronchiolar epithelium did not exhibit a hybridization signal for PRRSV nucleic acids. The anterior and middle lobes of the lung were more reliable than the caudal or accessory lobes for the detection of PRRSV nucleic acids. The in-situ hybridization technique used was rapid, specific and sensitive, and may prove useful for the diagnosis of PRRSV infection in routinely fixed and processed tissues.


Assuntos
Pulmão/química , Ácidos Nucleicos/análise , Síndrome Respiratória e Reprodutiva Suína/genética , Vírus da Síndrome Respiratória e Reprodutiva Suína/genética , Doenças dos Suínos/genética , Animais , Animais Recém-Nascidos , Hibridização In Situ , Pulmão/patologia , Síndrome Respiratória e Reprodutiva Suína/patologia , RNA Viral/análise , Suínos , Doenças dos Suínos/patologia
9.
J Vet Med Sci ; 63(5): 567-71, 2001 May.
Artigo em Inglês | MEDLINE | ID: mdl-11411506

RESUMO

Polymerase chain reaction (PCR)-based restriction fragment length polymorphism (RFLP) analysis was developed for directly typing porcine reproductive and respiratory syndrome virus (PRRSV) from lung specimens without virus isolation. Twenty nine lung specimens collected from postweaning pigs were isolated for PRRSV. When the PCR products from the 29 lung specimens were digested by the restriction enzymes MluI, HincII, SacII and HaeIII, the RFLP patterns from the 29 lung specimens matched with those from the corresponding PRRSV isolates from each pig. The results suggest that the PCR-based RFLP analysis method may be useful to distinguish PRRSV isolates directly from lung specimens without virus isolation.


Assuntos
Pulmão/virologia , Síndrome Respiratória e Reprodutiva Suína/virologia , Vírus da Síndrome Respiratória e Reprodutiva Suína/genética , Reação em Cadeia da Polimerase Via Transcriptase Reversa/veterinária , Animais , DNA Complementar/química , DNA Complementar/genética , Coreia (Geográfico) , Polimorfismo de Fragmento de Restrição , Vírus da Síndrome Respiratória e Reprodutiva Suína/química , RNA Viral/química , RNA Viral/genética , RNA Viral/isolamento & purificação , Sensibilidade e Especificidade , Suínos
10.
J Vet Med Sci ; 63(3): 341-2, 2001 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-11307940

RESUMO

The in vitro susceptibilities of 76 isolates of Actinobacillus pleuropneumoniae collected from pigs with pleuropneumonia were tested with 12 commonly used antimicrobial drugs by an agar dilution minimal inhibitory concentration procedure according to National Committee for Clinical Laboratory Standards (NCCLS) guidelines. Field isolates had low MICs for ceftiofur, danofloxacin and penicillin. No correlation of antimicrobial resistance was related to serotype.


Assuntos
Infecções por Actinobacillus/veterinária , Actinobacillus pleuropneumoniae/efeitos dos fármacos , Fluoroquinolonas , Pleuropneumonia/veterinária , Doenças dos Suínos/microbiologia , Infecções por Actinobacillus/tratamento farmacológico , Infecções por Actinobacillus/microbiologia , Actinobacillus pleuropneumoniae/isolamento & purificação , Animais , Anti-Infecciosos/farmacologia , Anti-Infecciosos/uso terapêutico , Cefalosporinas/farmacologia , Cefalosporinas/uso terapêutico , Resistência Microbiana a Medicamentos , Coreia (Geográfico) , Testes de Sensibilidade Microbiana/veterinária , Penicilinas/farmacologia , Penicilinas/uso terapêutico , Pleuropneumonia/tratamento farmacológico , Pleuropneumonia/microbiologia , Suínos , Doenças dos Suínos/tratamento farmacológico
11.
J Vet Med Sci ; 57(5): 951-3, 1995 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-8593311

RESUMO

A respiratory disorder was noted in a 5-year-old female orangutan kept in the Yongin Farmland. Radiographically, multiple radiodense foci ranging from 2 to 6 mm diameter were seen throughout the lung lobes. Grossly, the thoracic cavity revealed a firm texture and grayish-pink discoloration of the left apical lung lobe. Histopathologically, multifocal areas of granulomatous pneumonia present the right and left apical lung lobes. Both primers from IS1081 and IS6110 targeting 196 bp and 245 bp respectively were used in polymerase chain reaction, Mycobacterium tuberculosis was isolated from liver and confirmed by polymerase chain reaction.


Assuntos
Doenças dos Símios Antropoides/microbiologia , Mycobacterium tuberculosis/isolamento & purificação , Pongo pygmaeus/microbiologia , Tuberculose/veterinária , Animais , Doenças dos Símios Antropoides/epidemiologia , Doenças dos Símios Antropoides/patologia , Sequência de Bases , Primers do DNA/análise , Primers do DNA/química , Primers do DNA/genética , DNA Bacteriano/análise , DNA Bacteriano/química , DNA Bacteriano/genética , Feminino , Coreia (Geográfico)/epidemiologia , Fígado/química , Fígado/microbiologia , Pulmão/diagnóstico por imagem , Pulmão/microbiologia , Pulmão/patologia , Dados de Sequência Molecular , Reação em Cadeia da Polimerase , Radiografia , Tuberculose/epidemiologia , Tuberculose/microbiologia , Tuberculose/patologia
12.
Vet Rec ; 147(8): 215-8, 2000 Aug 19.
Artigo em Inglês | MEDLINE | ID: mdl-10994923

RESUMO

A panel of three anti-glycoprotein 5 (gp5) protein monoclonal antibodies (mAbs) (15, 28 and 246) and three anti-nucleocapsid (N) protein mAbs (SDOW17, VO17 and EP147) was used to investigate, by an indirect fluorescent antibody test, the antigenic variations of 50 Korean isolates of porcine reproductive and respiratory syndrome virus (PRRSV), and compare them with a us ATCC vR2332-derived attenuated vaccine strain and the reference European Lelystad strain of PRRSV. A multiplex PCR assay for the differentiation of European and North American genotypes of PRRSV was used to determine the genotype of the 50 Korean isolates. Forty-six (92 per cent) of the 50 Korean isolates shared the epitopes recognised by the anti-N protein mAb SDOW17. No reactivity to the anti-gp5 and anti-N protein mAbs was observed with the other four isolates. Six distinct patterns could be identified on the basis of their reactivities with the anti-PRRSV mAbs. All 50 isolates were identified as North American genotypes by the differential PCR.


Assuntos
Variação Antigênica/genética , Vírus da Síndrome Respiratória e Reprodutiva Suína/genética , Animais , Eletroforese em Gel de Ágar , Europa (Continente) , Genótipo , Coreia (Geográfico) , Reação em Cadeia da Polimerase , Vírus da Síndrome Respiratória e Reprodutiva Suína/isolamento & purificação , Suínos
13.
Vet Rec ; 143(15): 417-20, 1998 Oct 10.
Artigo em Inglês | MEDLINE | ID: mdl-9807791

RESUMO

A retrospective study was made of natural infections with Isospora suis in nursing piglets, recorded from April 1994 to May 1997, to determine the prevalence, microscopical lesions and other microorganisms associated with coccidiosis. One hundred and five (17.3 per cent) of the 605 nursing piglets submitted from 304 pig farms were diagnosed positive for coccidiosis. The affected piglets were from seven to 20 days old, with a mean age of 11.1 days. Coccidiosis occurred in each year but the incidence peaked in July (15 cases, 14.3 per cent), September (15 cases, 14.3 per cent), October (16 cases, 15.2 per cent) and November (18 cases, 17.1 per cent) and was lowest in May (no cases), August (two cases, 1.9 per cent) and June (four cases, 3.8 per cent). Histopathologically, villous atrophy resulting from the necrosis and sloughing of epithelial cells was a prominent feature of infection with I suis. In 49.5 per cent of the nursing piglets, other enteropathogens were identified, Escherichia coli (47.6 per cent) and transmissible gastroenteritis virus (3.8 per cent) being the most commonly diagnosed. Forty-five of 50 E coli isolates associated with coccidiosis tested negative by polymerase chain reaction for enterotoxigenic virulence factors, such as fimbriae and enterotoxins.


Assuntos
Coccidiose/veterinária , Isospora/patogenicidade , Doenças dos Suínos/epidemiologia , Animais , Coccidiose/epidemiologia , Coccidiose/patologia , Escherichia coli , Infecções por Escherichia coli/etiologia , Infecções por Escherichia coli/patologia , Gastroenteropatias/patologia , Gastroenteropatias/veterinária , Incidência , Coreia (Geográfico)/epidemiologia , Reação em Cadeia da Polimerase , Prevalência , Estudos Retrospectivos , Suínos , Doenças dos Suínos/parasitologia , Doenças dos Suínos/patologia
14.
Cell Death Differ ; 20(8): 1031-42, 2013 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-23645207

RESUMO

Proliferation and fusion of myoblasts is a well-orchestrated process occurring during muscle development and regeneration. Although myoblasts are known to originate from muscle satellite cells, the molecular mechanisms that coordinate their commitment toward differentiation are poorly understood. Here, we present a novel role for the transcription factor Forkhead box protein C2 (Foxc2) in regulating proliferation and preventing premature differentiation of activated muscle satellite cells. We demonstrate that Foxc2 expression is upregulated early in activated mouse muscle satellite cells and then diminishes during myogenesis. In undifferentiated C2C12 myoblasts, downregulation of endogenous Foxc2 expression leads to a decrease in proliferation, whereas forced expression of FOXC2 sustains proliferation and prevents differentiation into myotubes. We also show that FOXC2 induces Wnt signaling by direct interaction with the Wnt4 (wingless-type MMTV integration site family member-4) promoter region. The resulting elevated expression of bone morphogenetic protein-4 (Bmp4) and RhoA-GTP proteins inhibits the proper myoblast alignment and fusion required for myotube formation. Interestingly, continuous forced expression of FOXC2 alters the commitment of C2C12 myoblasts toward osteogenic differentiation, which is consistent with FOXC2 expression observed in patients with myositis ossificans, an abnormal bone growth within muscle tissue. In summary, our results suggest that (a) Foxc2 regulates the proliferation of multipotent muscle satellite cells; (b) downregulation of Foxc2 is critical for myogenesis to progress; and (c) sustained Foxc2 expression in myoblast cells suppresses myogenesis and alters their lineage commitment toward osteogenesis by inducing the Wnt4 and Bmp4 signaling pathways.


Assuntos
Proteína Morfogenética Óssea 4/fisiologia , Fatores de Transcrição Forkhead/fisiologia , Músculo Esquelético/fisiologia , Osteogênese/fisiologia , Regeneração/fisiologia , Proteína Wnt4/fisiologia , Animais , Diferenciação Celular/fisiologia , Linhagem Celular , Proliferação de Células , Fibroblastos/citologia , Fibroblastos/fisiologia , Camundongos , Camundongos Endogâmicos C57BL , Modelos Animais , Músculo Esquelético/citologia , Proteína MyoD/fisiologia , Mioblastos Esqueléticos/citologia , Mioblastos Esqueléticos/fisiologia , Células NIH 3T3 , Fator de Transcrição PAX7/fisiologia , Transdução de Sinais/fisiologia
15.
Clin Microbiol Infect ; 17(2): 232-5, 2011 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-20384698

RESUMO

A Korean nationwide surveillance on circulating rotavirus strains was conducted from September 2000 to August 2007 aiming to obtain prevaccine data for predicting vaccine effectiveness. The predominant strains among the 2779 strains analyzed varied annually and only approximately 50% had either a G or a P antigen present in both RotaTeq (Merck & Co. Inc., Whitehouse Station, NJ, USA) and Rotarix (GlaxoSmithKline, Brentford, UK).


Assuntos
Infecções por Rotavirus/epidemiologia , Infecções por Rotavirus/virologia , Rotavirus/classificação , Rotavirus/genética , Genótipo , Humanos , República da Coreia/epidemiologia , Rotavirus/isolamento & purificação , Vacinas contra Rotavirus/imunologia
16.
Clin Microbiol Infect ; 17(3): 404-8, 2011 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-20491833

RESUMO

The present study was conducted to survey the prevalence and genotypic distribution of human astrovirus (HAstV) circulating in South Korea. Of 160,027 patients with acute gastroenteritis, 2,057 (1.3%) were positive for HAstV antigen. We determined the genotypes of 187 HAstV strains collected from laboratories across the country. Genetic analysis revealed genotype 1 to be the most prevalent, accounting for 72.19% of the strains, followed by genotypes 8 (9.63%), 6 (6.95%), 4 (6.42%), 2 (3.21%) and 3 (1.60%). Our findings indicate that HAstV is less common but, even so, a potentially important viral agent of gastroenteritis in South Korea, with significant genetic diversity among circulating HAstV strains.


Assuntos
Infecções por Astroviridae/virologia , Variação Genética , Mamastrovirus/genética , Infecções por Astroviridae/epidemiologia , Genótipo , Humanos , Epidemiologia Molecular , Filogenia , República da Coreia/epidemiologia
20.
Epidemiol Infect ; 134(1): 87-93, 2006 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-16409654

RESUMO

To investigate the causal relationship of blood clotting factors and hepatitis A virus (HAV) infection in haemophilia patients during 1998-1999 in Korea, we performed a 1:3 matched case-control study and molecular detection of HAV from clotting factors and patients. The epidemiological investigation showed that one lot of clotting factor VIII was related epidemiologically to patients with hepatitis A with an odds ratio of 35.0, or 38.4 when adjusted for the interval between injections. We examined 17 sera collected from seven patients and 124 lots of blood clotting factors (factor VIII and factor IV) by HAV reverse transcriptase-polymerase chain reaction (RT-PCR). HAV RNA was detected in five clotting factors and six sera. The HAV sequence of one of the factor VIII samples was identical to the sequences found in three patients' sera. Findings from the laboratory and epidemiological studies suggested that the clotting factor was causally related to HAV infection in three haemophilia patients.


Assuntos
Cálcio/análise , Contaminação de Medicamentos , Fator VIII/análise , Hemofilia A/complicações , Vírus da Hepatite A/isolamento & purificação , Hepatite A/etiologia , Adolescente , Adulto , Idoso , Estudos de Casos e Controles , Criança , Pré-Escolar , Estudos Epidemiológicos , Feminino , Hemofilia A/tratamento farmacológico , Vírus da Hepatite A/genética , Vírus da Hepatite A/patogenicidade , Humanos , Lactente , Recém-Nascido , Coreia (Geográfico) , Masculino , Pessoa de Meia-Idade , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Fatores de Risco
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