Impacts of nitrogen practices on yield, grain quality, and nitrogen-use efficiency of crops and soil fertility in three paddy-upland cropping systems.

BACKGROUND: Global food security faces a number of challenges due to increasing population, climate change, and urbanization, while excessive use of nitrogen fertilizers has become a major challenge for sustainable, intensive agriculture. Assessing the impact of agronomic management practices on seed yield, grain quality, and soil fertility is a critical step in understanding nutrientuse efficiency. RESULT: The comprehensive evaluation index had good fitness to that of single attribute (i.e. seed yield, crop quality and soil fertility), indicating that the comprehensive evaluation index was reliable. Applying controlled-release urea (rice in wheat and oilseed rape field: 150 kg N ha-1 , other crops: 120 kg N ha-1 ) plus common urea (30 kg N ha-1 ) incorporating straw from the previous season across the growing season for cereal and oilseed crops showed a slight improvement in seed productivity and Nuse efficiency among three cropping systems in the traditional evaluation method. Compared with local farm practice (applying common urea of 150 kg N ha-1 ), applying these practices in combination based on the outcome of the comprehensive evaluation index method decreased the seed yield by -1.27 ~ 29.8% but improved quality and soil fertility for the paddy-upland cropping system, respectively. CONCLUSION: Properly managing N application by applying partial and fully controlled release of urea with or without straw incorporation for a specific crop system has the potential to provide a better compromise among yield, grain quality, and soil fertility in southern China. © 2020 Society of Chemical Industry.

Identifying risk factors for exposure to culturable allergenic moulds in energy efficient homes by using highly specific monoclonal antibodies.

The aim of this study was to determine the accuracy of monoclonal antibodies (mAbs) in identifying culturable allergenic fungi present in visible mould growth in energy efficient homes, and to identify risk factors for exposure to these known allergenic fungi. Swabs were taken from fungal contaminated surfaces and culturable yeasts and moulds isolated by using mycological culture. Soluble antigens from cultures were tested by ELISA using mAbs specific to the culturable allergenic fungi Aspergillus and Penicillium spp., Ulocladium, Alternaria, and Epicoccum spp., Cladosporium spp., Fusarium spp., and Trichoderma spp. Diagnostic accuracies of the ELISA tests were determined by sequencing of the internally transcribed spacer 1 (ITS1)-5.8S-ITS2-encoding regions of recovered fungi following ELISA. There was 100% concordance between the two methods, with ELISAs providing genus-level identity and ITS sequencing providing species-level identities (210 out of 210 tested). Species of Aspergillus/Penicillium, Cladosporium, Ulocladium/Alternaria/Epicoccum, Fusarium and Trichoderma were detected in 82% of the samples. The presence of condensation was associated with an increased risk of surfaces being contaminated by Aspergillus/Penicillium spp. and Cladosporium spp., whereas moisture within the building fabric (water ingress/rising damp) was only associated with increased risk of Aspergillus/Penicillium spp. Property type and energy efficiency levels were found to moderate the risk of indoor surfaces becoming contaminated with Aspergillus/Penicillium and Cladosporium which in turn was modified by the presence of condensation, water ingress and rising damp, consistent with previous literature.