Identification of a new phytotoxic compound from culture filtrates of an aggressive Rhizoctonia solani AG 1A isolate inducing sheath blight of rice.

Phytotoxins produced by Rhizoctonia solani AG1-1A (Anastomosis Group 1 Subgroup 1A) play a significant role in developing sheath blight disease in rice. A phytotoxin in the partially purified ethyl acetate fraction from the culture filtrate of a highly aggressive R. solani (RIRS-K) isolate, with Indian Type Culture Collection (ITCC) number 7479, infecting rice that could incite necrotic symptoms characteristic of the fungus was identified. The role of the crude toxin in the pathogenicity and virulence of the fungal pathogen on rice was first established by artificial inoculation assay under controlled conditions. The crude ethyl acetate extract obtained from the culture filtrate of RIRS-K was first fractionated by column chromatography. Further purification of the bioactive fraction was carried out by using bioassay-guided fractionation, and a toxic fraction was obtained. The most bioactive fraction was analyzed by GC-MS analysis, and 3-butylpyridine (3-BP) was identified as a major compound in the active fraction by comparing its mass spectrum with NIST library and its standard. The purified bioactive fraction and standard (3-BP) toxicity was further validated and compared at 1000 ppm. The result showed that both the bioactive fraction and the 3-BP have caused necrosis, similar to the one incited by R. solani. This study showed that 3-BP is one of the major compounds responsible for the necrosis development in the rice plant during ShB disease and is a hitherto unexplored toxin of R. solani in rice.

Draft genome sequence of Bacillus safensis strain WOIS2 KX774194, a nitrile-metabolizing bacterium isolated from solid waste leachates at Olusosun dumpsite, Ojota, Lagos State, Nigeria.

Bacillus safensis strain WOIS2, a nitrile-metabolizing bacterium, was isolated from solid waste leachates at the Olusosun dumpsite, Ojota, Lagos State, Nigeria. Here, we present the draft genome sequence of strain WOIS2. These data provide valuable information on the bioprospecting of B. safensis nitrilase and other intriguing genes of interest.

Unveiling genome sequence of Bacillus safensis strain WOB7 KX774196, a linamarin-utilizing bacterium (LUB) isolated from cassava wastewater (CWW) in Lagos State, Nigeria.

Bacillus safensis strain WOB7 is a linamarin-utilizing bacterium (LUB) that was isolated from cassava wastewater obtained from a processing factory. We present here the draft genome sequence of the strain (WOB7). These data provide valuable information on the prospects of the linamarase and other genes of importance associated with cyanogen detoxification.

Analysis of the genome of Bacillus safensis strain WOB3 KX774195, a Linamarin-utilizing bacterium (LUB) isolated from Cassava wastewater (CWW), Lagos State, Nigeria.

Linamarin-utilizing bacterium (LUB) is a microorganism that uses and breaks down cassava's principal cyanogenic compound, linamarin. Here, we present the draft genome sequence of Bacillus safensis strain WOB3 (previously Bacillus pumilus strain WOB3) sequenced and assembled with a total reads of 8,750,054 bp. The genome has 1,269 contigs and, G+C content of 41.55%. The genome has 4,749 total genes, 4,614 protein-coding sequences (CDSs), 3, 8 and 10 rRNA genes, 74 tRNA genes, and 5 ncRNA genes. This whole genome shotgun project has been deposited in GenBank under accession number JAYSGU000000000.