RESUMO
BACKGROUND: The genus Monascus includes several species of fungi valued across Asia for their culinary uses and diverse medicinal properties. In this study, we evaluated the applicability of random amplified polymorphic DNA (RAPD) and inter-simple sequence repeats (ISSR) markers in characterizing the genetic diversity in 41 Monascus strains collected from various regions of Fujian Province, the leading producer of Monascus in China. RESULTS: Seven screened ISSR primers generated 56 polymorphic bands, of which 93.33% were polymorphic. The genetic similarity coefficients (GSC) of the strains ranged from 0.50 to 1.00. Comparative sequence analysis using seven screened RAPD primers amplified a total of 49 polymorphic bands, of which 81.67% were polymorphic; GSC values ranged from 0.62 to 1.00. CONCLUSION: Correlation analysis revealed a significant positive correlation in genetic distances assessed using above two markers, which indicated they were suitable for Monascus species characterization. ISSR markers were more suitable for the classification and determination of Monascus species, while RAPD markers appear to be preferable for analyzing the differences among strains within the same species. Our study revealed that Monascus possesses rich genetic diversity, and that the genetic relationships among the selected strains were, to a very limited extent, correlated to their geographical variation. © 2016 Society of Chemical Industry.
Assuntos
Monascus/genética , Polimorfismo Genético , China , Análise por Conglomerados , DNA Fúngico/química , DNA Fúngico/metabolismo , Marcadores Genéticos , Monascus/classificação , Monascus/crescimento & desenvolvimento , Monascus/isolamento & purificação , Micologia/métodos , Filogenia , Filogeografia/métodos , Técnica de Amplificação ao Acaso de DNA Polimórfico , Sequências Repetitivas de Ácido NucleicoRESUMO
OBJECTIVE: To establish the fingerprint spectrum of Loquat Leaf of Fujian authentic medicinal herbs by HPCE. METHODS: The HPLC fingerprints of Loquat Leaf were obtained from instrument HP1100. The HPLC separation was performed on a Eclipse XDB-C18 (4.6 mm x 150 mm, 5 microm) analytical column diluted with methanol-l% glacial acetic acid (90:10) at the flow rate of 1.0 ml/min. The temperature of column was room temperature. The UV detection wavelength was 215 nm. RESULTS: Detected 12 batches samples of different species Lopuat Leaf Fingerprint by the method of HPLC. Eight peaks in the chromatogram were common. All the similarity were above 0.9, the fingerprints integer feature were coincident essentially. CONCLUSION: The method is convenient, quick and exact, and its reproducibility is good. It will be a scientific reference for qualitation identification and quality evaluation of Loquat Leaf.