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1.
Cancer Res ; 35(10): 2836-44, 1975 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-125626

RESUMO

Rats were given a single dose of aflatoxin B1 lethal to 50% of the animals (7.20 mg/kg). Their livers were examined histochemically in correlation with sequential histological lesions. Early periportal liver cell necrosis and marked biliary cell proliferation were observed. Periportal cytoplasmic glycogen and RNA depletion occurred during this early period and subsequently extended to the whole lobule. The enzymes investigated decreased or disappeared in the periportal area; but alkaline phosphatase increased strikingly in the centrolobular area, whereas canalicular adenosinetriphosphatase completely disappeared throughout the liver lobule. The histochemical changes reverted to normal after cessation of the necrosis. Histochemical techniques were more sensitive in detecting the vulnerability of the periportal parenchyma to aflatoxin. After the necrosis, regenerative foci appeared. They showed a variable content in glycogen and RNA and were characteristically enzyme deficient. This reflects the immaturity of regenerating hepatocytes. These early foci subsequently disappeared and are thus considered irrelevant to hepatomagenesis.


Assuntos
Aflatoxinas/intoxicação , Fígado/efeitos dos fármacos , Adenosina Trifosfatases/metabolismo , Fosfatase Alcalina/metabolismo , Animais , Ductos Biliares Intra-Hepáticos/patologia , Divisão Celular , Doença Hepática Induzida por Substâncias e Drogas/metabolismo , Doença Hepática Induzida por Substâncias e Drogas/patologia , Glicogênio/metabolismo , Fígado/metabolismo , Regeneração Hepática , Masculino , RNA/metabolismo , Ratos
2.
Cancer Res ; 35(10): 2845-52, 1975 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-168970

RESUMO

Male Wistar rats were given 50 mug of aflatoxin B1 twice a week for 4 weeks, and thereafter 75 mug twice a week for 10 weeks. Their livers were investigated histologically and histochemically for glycogen, RNA, fat, alkaline and acid phosphatases, adenosine triphosphatase, 5'-nucleotidase, glucose-6-phosphatase, glucose-6-phosphate dehydrogenase, succinic dehydrogenase, and alkaline and acid nucleases. No significant lesions occurred before 15 weeks. During this period, the liver was histochemically unchanged except for a periportal decrease of alkaline phosphatase and adenosine triphosphatase. Scattered hepatocytes with a strong glucose-6-phosphatase activity appeared. These changes represent toxic effects of aflatoxin B1 and are irrelevant to carcinogenesis. From 15 weeks onward, three types of liver cell hyperplastic foci and nodules developed. Histologically, and with respect to glycogen, fat, and RNA content, only two of these types were considered as potential precursors of hepatocarcinomas. However, all types exhibited a decrease or absence of the enzymes studied. Both histological and histochemical changes stressed the complex heterogeneity existing between and within hepatic foci and nodules. From 11 months on, hepatocarcinomas developed. The tumors disclosed similar histochemical changes. This similarity further supports the "precarcinomatous" nature of hyperplastic foci and nodules. It appears that focal changes in surface as well as in cytoplasmic and nuclear enzymes are intimately and very early linked to the carcinogenic process. Whether they are fundamental or only represent an epiphenomenon remains unclear.


Assuntos
Aflatoxinas , Neoplasias Hepáticas/induzido quimicamente , Fosfatase Ácida/metabolismo , Adenosina Trifosfatases/metabolismo , Fosfatase Alcalina/metabolismo , Animais , Doença Hepática Induzida por Substâncias e Drogas/patologia , Glucose-6-Fosfatase/metabolismo , Glucosefosfato Desidrogenase/metabolismo , Glicogênio/metabolismo , Metabolismo dos Lipídeos , Neoplasias Hepáticas/metabolismo , Neoplasias Hepáticas/patologia , Masculino , Neoplasias Experimentais/induzido quimicamente , Nucleotidases/metabolismo , Lesões Pré-Cancerosas/metabolismo , Lesões Pré-Cancerosas/patologia , RNA Neoplásico/metabolismo , Ratos , Succinato Desidrogenase/metabolismo , Fatores de Tempo
3.
J Immunol Methods ; 6(3): 283-9, 1975 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-46904

RESUMO

Hepatitis B antigen (HBAg) has been demonstrated by the indirect immunofluorescent technique and by orcein staining in 20 liver biopsies fixed in Bouin's fixative and embedded in paraffin. The results were compared with those obtained previously by immunogluorescence on frozen sections of the same biopsies. Ten biopsies which were positive in frozen sections were also positive by immunofluorescence in parafin sections, whereas only six were positive by orcein staining. In orcein-stained sections, the cellular localization of HBAg was precisely in the same places as in the slides examined by immunogluorescence. The intessity of the fluorescence in paraffin sections was almost the same as in frozen sections. The localization of the antigen was histologically more precise in paraffin sections. Besides various advantages, indlucing aboidance of freezing aquipment and procedures, paraffin sections are more easy to handle and biopsies from distant hospitals can be processed. The advantages of the immunofluorescent test in comparison to orcein staining are its immunological specificity and higher sensitivity.


Assuntos
Imunofluorescência/métodos , Antígenos da Hepatite B/análise , Fígado/imunologia , Biópsia , Congelamento , Humanos , Parafina , Coloração e Rotulagem
4.
Am J Surg Pathol ; 25(11): 1388-96, 2001 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-11684955

RESUMO

Hepatocellular adenoma is a benign tumor of the liver that has a small but not negligible risk of malignant transformation into hepatocellular carcinoma. In analogy with the established role of oval cells in hepatocarcinogenesis in rodent models, human hepatic progenitor cells may have a function in the development of liver tumors. To investigate this issue, we performed immunohistochemistry on biopsies of 10 consecutively resected hepatocellular adenomas using markers for hepatic progenitor cells. Sections of paraffin-embedded and frozen biopsies were stained using antibodies against cytokeratins 7, 8, 18, and 19, chromogranin-A, OV-6, and neural cell adhesion molecule. Hepatic progenitor cells were observed in five of 10 hepatocellular adenomas. These five tumors also contained cells with an immunohistochemical phenotype intermediate between hepatic progenitor cells and hepatocytes. Hepatic progenitor cells and intermediate hepatocyte-like cells were scattered throughout the tumors with a density that varied from area to area. Ultrastructural examination confirmed the presence of hepatic progenitor cells. Our study shows that hepatic progenitor cells are present in a considerable proportion of hepatocellular adenomas, supporting the hypothesis that human hepatic progenitor cells can play a role in the development of hepatocellular tumors.


Assuntos
Adenoma de Células Hepáticas/patologia , Hepatócitos/patologia , Neoplasias Hepáticas/patologia , Células-Tronco/patologia , Adenoma de Células Hepáticas/química , Adenoma de Células Hepáticas/etiologia , Adenoma de Células Hepáticas/ultraestrutura , Adulto , Antígenos de Diferenciação/análise , Contagem de Células , Cromogranina A , Cromograninas/análise , Desmossomos/ultraestrutura , Feminino , Técnica Indireta de Fluorescência para Anticorpo , Hepatócitos/química , Hepatócitos/ultraestrutura , Humanos , Imuno-Histoquímica , Queratinas/análise , Neoplasias Hepáticas/química , Neoplasias Hepáticas/etiologia , Neoplasias Hepáticas/ultraestrutura , Masculino , Microscopia Eletrônica , Pessoa de Meia-Idade , Moléculas de Adesão de Célula Nervosa/análise , Células-Tronco/química , Células-Tronco/ultraestrutura
5.
Am J Surg Pathol ; 10(11): 780-8, 1986 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-2946246

RESUMO

Using in-situ immuno- and enzymehistochemical techniques, the phenotype of the neoplastic cells in seven cases of mantle zone lymphoma (MZL) was compared to that in seven cases of nodular poorly-differentiated lymphocytic lymphoma (NPDLL). The neoplastic nodules in MZL consisted of medium-sized lymphoid cells with slightly irregular nuclei and finely dispersed chromatin, expressing monoclonal surface IgM or IgM plus IgD, and displaying membranous alkaline phosphatase (ALP) activity. These cells proliferated around follicular centers that demonstrated a polyclonal pattern of reactivity for both types of light chains and a distorted meshwork of dendritic reticulum cells. The neoplastic nodules in NPDLL consisted of small lymphoid cells with markedly irregular nuclei and coarsely granulated chromatin, expressing monoclonal surface IgM and lacking ALP-activity. These tumor cells also frequently expressed transferrin receptor and common acute lymphoblastic leukemia-antigen (CALLA). The neoplastic nodules showed an undistorted meshwork of dendritic reticulum cells, and were occasionally bordered by remnants of polyclonal lymphocytic coronas. These results confirm the previous suggestion that NPDLL arises from a cell type that is a normal constituent of follicular centers, whereas MZL arises from the lymphocytic corona. The morphological, enzyme- and immunohistochemical features of MZL cells strongly suggest that MZL arises from marginal zone lymphocytes, a subset of corona lymphocytes that expresses ALP-activity, high IgM and low IgD-levels.


Assuntos
Linfonodos/patologia , Linfoma/patologia , Adulto , Idoso , Fosfatase Alcalina/metabolismo , Anticorpos Monoclonais , Anticorpos Antineoplásicos/análise , Antígenos de Neoplasias/análise , Feminino , Humanos , Imunoglobulinas/análise , Linfócitos/classificação , Linfoma/enzimologia , Linfoma/genética , Linfoma/imunologia , Linfoma Folicular/patologia , Masculino , Pessoa de Meia-Idade , Neprilisina , Fenótipo
6.
Am J Surg Pathol ; 14(2): 101-12, 1990 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-2301697

RESUMO

The medium-sized mononuclear cell with plasmacytoid features, formerly known as "T-associated plasma cell" or "plasmacytoid T cell," has recently been shown to express several myelomonocyte and monocyte-macrophage associated antigens, suggesting a monocytic origin, and it has been renamed "plasmacytoid monocyte." The present study describes the clinical and pathological features of two patients with generalized lymphadenopathy and leukemia (chronic myelomonocytic leukemia in Case 1, and acute non-B, non-T lymphoblastic leukemia in Case 2), and whose lymph node biopsies showed large numbers of plasmacytoid monocytes associated with the leukemic infiltrates. Case 1 was strikingly similar to three previously reported cases of so-called "plasmacytoid T cell" lymphoma, all associated with a myeloproliferative disorder. In our case, the destructive growth pattern of the plasmacytoid monocytes and the de novo expression of CD5 on these cells favored their neoplastic nature; the sharing of some markers of plasmacytoid monocytes with the myelomonocytic infiltrate suggested they were part of the tumoral proliferation. In Case 2, plasmacytoid monocytes displayed an immunophenotype guide similar to that reported in reactive conditions and were antigenically unrelated to the leukemic cells; plasmacytoid monocyte clusters occurred also in the lymphoid parenchyma spared by the leukemic infiltrate. These findings led us to interpret the large numbers of plasmacytoid monocytes in this second case as a tumor-associated host reaction.


Assuntos
Leucemia Mielomonocítica Crônica/patologia , Linfonodos/patologia , Monócitos/patologia , Leucemia-Linfoma Linfoblástico de Células Precursoras/patologia , Idoso , Anticorpos Monoclonais , Antígenos de Diferenciação de Linfócitos T/análise , Feminino , Humanos , Leucemia Mielomonocítica Crônica/imunologia , Masculino , Fenótipo , Leucemia-Linfoma Linfoblástico de Células Precursoras/imunologia
7.
J Histochem Cytochem ; 37(4): 551-4, 1989 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-2926130

RESUMO

We report on the binding of biotin, and hence of biotinylated antibodies and lectins, to ground glass hepatocytes and liver cell membranes in chronic hepatitis B viral infection. This binding is of low affinity, and was proved to be directed at the hepatitis B surface antigen, presumably at its disulfide bonds. To avoid false-positive results, this affinity should be considered in the interpretation of immunohistochemical stainings of hepatitis B virus-infected liver tissue with biotinylated reagents.


Assuntos
Biotina/metabolismo , Antígenos de Superfície da Hepatite B/metabolismo , Anticorpos Monoclonais , Biópsia , Membrana Celular/imunologia , Membrana Celular/metabolismo , Reações Cruzadas , Antígenos de Superfície da Hepatite B/imunologia , Humanos , Imuno-Histoquímica , Fígado/citologia , Fígado/metabolismo , Fígado/patologia
8.
J Histochem Cytochem ; 33(9): 959-64, 1985 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-3860562

RESUMO

Various fixation and staining procedures for the demonstration of surface and cytoplasmic antigens have been described. An immunostaining procedure was sought that would allow the demonstration of these antigens, especially in small human tissue samples at the ultrastructural level. A modification and adaptation of the technique of Eldred, Zucker, Karten, and Yazula (J Histochem Cytochem 31:285, 1983) was applied on several varieties of human tissue, including liver, skin, and lymphoid tissue, using monoclonal and polyclonal antibodies in an indirect peroxidase procedure. In this way a reliable and generally applicable procedure was developed that satisfied the following demands: Use of a universal fixative that allows preservation of the antigenicity of various antigens; Adequate penetration of the tissue by the immunological reagents; Optimal preservation of subcellular structures; and Possibility to store the tissue samples for considerable periods of time.


Assuntos
Histocitoquímica/métodos , Fígado/ultraestrutura , Pele/ultraestrutura , Anticorpos Monoclonais , Antígenos/análise , Biópsia , Citoplasma/análise , Antígenos HLA-DR , Hepatite B/patologia , Antígenos de Histocompatibilidade Classe II/análise , Humanos , Linfoma/patologia , Microscopia Eletrônica
9.
Invest Ophthalmol Vis Sci ; 26(2): 220-5, 1985 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-3882607

RESUMO

The distribution of B- and T-lymphocytes, of OKIa positive cells, and of HLA-ABC antigens in the normal cornea was investigated using monoclonal antibodies. The lymphocytes and Langerhans cells are present mainly in the well-vascularized limbic region but also occur albeit in small number in the center of the cornea. HLA-ABC antigens are strongly expressed on the epithelial cells of the cornea and the limbus.


Assuntos
Córnea/citologia , Antígenos HLA/análise , Antígenos de Histocompatibilidade Classe II/análise , Células de Langerhans/imunologia , Linfócitos/classificação , Idoso , Linfócitos B/citologia , Córnea/imunologia , Lâmina Limitante Posterior/citologia , Células Epiteliais , Feminino , Antígenos HLA-A , Antígenos HLA-B , Antígenos HLA-C , Histocitoquímica , Humanos , Técnicas Imunoenzimáticas , Masculino , Pessoa de Meia-Idade , Linfócitos T/citologia
10.
Immunol Lett ; 20(4): 277-81, 1989 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-2785501

RESUMO

So-called plasmacytoid T cells represent a subset of monocyte related cells, which share with endothelium the CD36+ CD11b- (OKM5+ OKM1-) phenotype. The reactivity of plasmacytoid T cells with rat monoclonal antibody HECA-452, highly specific for high endothelial venules, was analyzed in reactive lymph nodes. In all cases, HECA-452 not only labelled the endothelium of high endothelial venules, but also strongly reacted with singular and clustered plasmacytoid T cells. The HECA-452 positivity for high endothelial venules and plasmacytoid T cells visualized a lymph node compartment extending from the subcapsular sinus to the corticomedullary junction. This compartment surrounded the composite nodule and was designated the "extranodular" compartment. The co-occurrence of plasmacytoid T cells and high endothelial venules in this extranodular compartment, together with their immunophenotypical similarities, may be indicative of functional co-operations.


Assuntos
Anticorpos Monoclonais , Endotélio Vascular/imunologia , Linfonodos/imunologia , Linfócitos T/imunologia , Reações Antígeno-Anticorpo , Humanos , Linfonodos/citologia , Monócitos/classificação , Monócitos/imunologia , Plasmócitos/imunologia , Vênulas
11.
Clin Liver Dis ; 2(2): 249-64, viii, 1998 May.
Artigo em Inglês | MEDLINE | ID: mdl-15560031

RESUMO

This article reviews the histopathologic liver alterations in chronic cholestatic diseases and parenchymal changes associated with periportal ductular reaction and fibrosis, which may result in end-stage biliary cirrhosis. Adult ductopenic diseases are possible causes of such chronic cholestasis. Ductopenia is defined, and guidelines are given for its quantitation. The last section summarizes the specific histologic lesions of primary biliary cirrhosis, primary sclerosing cholangitis, their histologic mimics, and the differential diagnosis with drug-induced cholestasis and ductopenia.


Assuntos
Doenças dos Ductos Biliares/patologia , Ductos Biliares Intra-Hepáticos/patologia , Colestase/patologia , Doenças Autoimunes/patologia , Colangite Esclerosante/patologia , Doença Crônica , Diagnóstico Diferencial , Humanos , Cirrose Hepática Biliar/patologia
12.
Mayo Clin Proc ; 73(1): 80-9, 1998 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-9443684

RESUMO

Intrahepatic bile ducts (IHBDs) develop from bipotential liver progenitor cells in contact with the mesenchyme of the portal vein and thus form the "ductal plates." The ductal plates are remodeled into mature tubular ducts. Lack of remodeling results in the persistence of periportal epithelial sleeves or "ductal plate malformation" (DPM). A proposal is that virtually all congenital diseases of IHBDs represent examples of DPM. Some early, severe types of extrahepatic bile duct atresia are characterized by DPM, a suggestion of a prenatal beginning of the disease. Several congenital diseases are characterized by dilatation of segments of IHBDs and variable degrees of fibrosis. Such "fibrocystic diseases" represent DPM at different levels of the biliary tree. Autosomal recessive polycystic kidney disease represents DPM of interlobular bile ducts, associated with tubular dilatation of collecting renal tubules. Congenital hepatic fibrosis may derive from the same type of liver lesion, through a superimposed destructive type of cholangiopathy associated with scarring fibrosis. Caroli's disease represents DPM of the larger IHBDs, whereas Caroli's syndrome combines the lesions of Caroli's disease and congenital hepatic fibrosis. von Meyenburg complexes represent DPM of smaller interlobular ducts; their dilatation gives rise to the liver cysts in autosomal dominant polycystic kidney disease. Finally, DPM is a component of the tissue abnormalities in so-called mesenchymal hamartoma.


Assuntos
Doenças dos Ductos Biliares/congênito , Ductos Biliares Intra-Hepáticos/anormalidades , Doenças dos Ductos Biliares/patologia , Atresia Biliar/etiologia , Doença de Caroli/etiologia , Fibrose/congênito , Humanos , Fígado/patologia , Rim Policístico Autossômico Dominante/etiologia
13.
Am J Med Genet ; 35(4): 532-5, 1990 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-2333885

RESUMO

We report on a boy with Alagille syndrome. Chromosome analysis on a peripheral blood lymphocyte culture showed a de novo deletion of the short arm of chromosome 20 with a 46,XY,del(20)(p11.2) chromosome constitution. This is the second report of a del(20p) in a patient with Alagille syndrome. The possible localisation of this autosomal dominant syndrome on 20p is discussed.


Assuntos
Anormalidades Múltiplas/diagnóstico , Deleção Cromossômica , Cromossomos Humanos Par 20 , Colestase , Face/anormalidades , Insuficiência de Crescimento , Humanos , Recém-Nascido , Linfócitos/citologia , Masculino , Estenose da Valva Pulmonar , Coluna Vertebral/anormalidades , Síndrome
14.
Hum Pathol ; 15(3): 293-5, 1984 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-6321324

RESUMO

Inclusions positive for periodic acid-Schiff, resistant to diastase, and immunoreactive to alpha-1-antitrypsin (AAT) were found in hepatocytes and pancreatic islet cells of a patient with clinical and pathologic features of AAT deficiency. Alpha-1-antitrypsin was detected in all pancreatic islets, and AAT-positive cells were observed in the excretory pancreatic ducts. These findings suggest that the pancreas synthesizes AAT and possibly serves as a "storage" place in AAT deficiency. Intercalated cells in the excretory pancreatic ducts may be an additional source of AAT.


Assuntos
Fígado/metabolismo , Pâncreas/metabolismo , alfa 1-Antitripsina/metabolismo , Carcinoma Hepatocelular/complicações , Feminino , Humanos , Ilhotas Pancreáticas/metabolismo , Ilhotas Pancreáticas/patologia , Fígado/patologia , Cirrose Hepática/complicações , Neoplasias Hepáticas/complicações , Pessoa de Meia-Idade , Enfisema Pulmonar/complicações , Deficiência de alfa 1-Antitripsina
15.
Hum Pathol ; 17(3): 301-7, 1986 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-2419238

RESUMO

Skin biopsy specimens from 12 elderly patients with Merkel cell tumors were investigated. Conventional light microscopy and immunohistochemical techniques were used. All of the tumors had similar morphologic features. Immunoreactivity for neuronspecific enolase, gastrin, calcitonin, and epithelial membrane-like antigen was demonstrated, and both neurofilaments and keratin filaments were observed. The immunohistochemical findings supported a Merkel cell origin for these Merkel cell tumors. The co-expression of neuroendocrine and epithelial markers in Merkel cell carcinomas is suggestive of neuroendocrine differentiation in a neoplasm of epithelial origin. Merkel cell carcinomas share many characteristics with neuroendocrine tumors of the bronchopulmonary and gastrointestinal tracts. All of these neoplasms may originate from cells of similar types that are present in several organs.


Assuntos
Neoplasias Cutâneas/patologia , Idoso , Feminino , Secções Congeladas , Histocitoquímica , Humanos , Técnicas Imunoenzimáticas , Queratinas/análise , Masculino , Pessoa de Meia-Idade , Fosfopiruvato Hidratase/análise , Proteínas S100/análise
16.
Hum Pathol ; 19(5): 562-8, 1988 May.
Artigo em Inglês | MEDLINE | ID: mdl-2453442

RESUMO

Normal human hepatocytes express cytokeratins no. 8 and 18, whereas bile duct cells contain the same cytokeratins and, in addition, cytokeratins no. 7 and 19. This cytokeratin pattern is believed to be preserved during neoplastic transformation. Thirty-four cases of hepatocellular carcinoma (11 well differentiated, 16 moderately differentiated, 7 poorly differentiated) were studied on frozen sections using monoclonal antisera directed against individual cytokeratins no. 7, 8, 18, and 19 in an immunoperoxidase procedure. In 17 of 34 cases, tumor cells showed only reactivity with monoclonals anticytokeratin no. 8 and 18. However, 17 of 34 cases showed an aberrant pattern in that a variable number of tumor cells were stained with anticytokeratins no. 7 and/or 19 in addition to no. 8 and 18. Only three of 11 well-differentiated cases displayed an unexpected cytokeratin pattern, whereas an aberrant pattern was present in all seven of seven poorly differentiated cases. These results are in conflict with previously published data obtained by two-dimensional gel electrophoresis and immunohistochemistry. They indicate that the cytokeratin pattern might not always be preserved during neoplastic transformation. The implication of this finding for the differential diagnosis of metastatic gastrointestinal carcinomas is discussed.


Assuntos
Carcinoma Hepatocelular/metabolismo , Queratinas/metabolismo , Neoplasias Hepáticas/metabolismo , Adolescente , Adulto , Idoso , Anticorpos Monoclonais , Carcinoma Hepatocelular/patologia , Feminino , Humanos , Imuno-Histoquímica , Neoplasias Hepáticas/patologia , Masculino , Pessoa de Meia-Idade
17.
Hum Pathol ; 16(10): 1009-14, 1985 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-3930382

RESUMO

The immunohistochemical findings from an investigation of suppurative granulomatous lymphadenitis (SGL) are presented. With a broad panel of monoclonal and polyclonal antibodies directed against B cells, T cells, monocytes/macrophages, HLA-DR antigens, and the transferrin receptor, early, nonsuppurative granulomas were found to consist of OKM1+ OKIa1+ OKT9+ epithelioid histiocytes and multinucleated giant cells, admixed with variable numbers of OKT4+ Leu-3a+ helper/inducer T cells. These nonsuppurative lesions were surrounded by distinctive cuffs of BA1+ B1+ sIgM+ sIgD+ OKIa1+ lymphocytes. In contrast, suppurative granulomas were bordered by palisades of OKM1+ OKIa1+ OKT9+ epithelioid histiocytes, admixed with some OKT8+ suppressor/cytotoxic T cells. These suppurative lesions lacked distinctive cuffs of B lymphocytes, but half of the lesions were surrounded by numerous plasma cells that expressed cytoplasmic IgA and IgG. Based on these immunohistochemical findings, it is concluded that a shift in the nature of the predominant intragranulomatous T-cell subset occurs during the successive phases of the immune response in SGL. The cause of the central necrosis and suppuration may be related to the excessive numbers of intragranulomatous OKT8+ T cells or to the formation of immune complexes by the surrounding plasma cells.


Assuntos
Granuloma/patologia , Linfonodos/patologia , Linfadenite/patologia , Anticorpos , Antígenos de Diferenciação de Linfócitos T , Antígenos de Superfície/análise , Linfócitos B/patologia , Doença da Arranhadura de Gato/diagnóstico , Doença da Arranhadura de Gato/patologia , Granuloma/imunologia , Histiócitos/patologia , Humanos , Hiperplasia , Linfadenite/imunologia , Infecções por Mycobacterium não Tuberculosas/diagnóstico , Infecções por Mycobacterium não Tuberculosas/patologia , Necrose , Supuração , Linfócitos T/classificação , Linfócitos T/patologia
18.
Hum Pathol ; 21(3): 302-8, 1990 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-1690171

RESUMO

Six cases of hepatoblastoma (five epithelial, one mixed epithelial-mesenchymal) were studied on serially cut cryostat sections, using a panel of monoclonal antibodies directed against individual cytokeratins, vimentin, and desmin, in an indirect immunoperoxidase procedure. Embryonic and fetal-type tumor cells expressed the "hepatocellular" cytokeratins no. 8 and 18 but, surprisingly, also expressed the "bile duct type" cytokeratin no. 19. In addition, two cases had a number of tumor cells which were also positive for the "bile duct type" cytokeratin no. 7. Cells embedded in osteoid-like material were immunoreactive for vimentin but also for cytokeratins no. 7, 18, and 19. Gel electrophoresis, and Western blotting of cytoskeletal extracts, confirmed the immunohistochemical data. The implications of these findings for the histogenesis of hepatoblastoma are discussed in this report.


Assuntos
Carcinoma Hepatocelular/metabolismo , Queratinas/metabolismo , Neoplasias Hepáticas/metabolismo , Adolescente , Adulto , Western Blotting , Carcinoma Hepatocelular/patologia , Criança , Pré-Escolar , Desmina/metabolismo , Eletroforese em Gel de Poliacrilamida , Feminino , Humanos , Imuno-Histoquímica , Lactente , Neoplasias Hepáticas/patologia , Masculino , Neoplasias Embrionárias de Células Germinativas/metabolismo , Neoplasias Embrionárias de Células Germinativas/patologia , Vimentina/metabolismo
19.
Hum Pathol ; 15(6): 559-65, 1984 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-6609875

RESUMO

In four cases of sarcoidal and five cases of tuberculous lymphadenitis, the cellular composition of hypersensitivity-type granulomas was investigated by the application of monoclonal and polyclonal antibodies to cryostat sections. The composition of the granulomas was the same in the two disorders; they consisted of centrally placed OKM1 + OKIa + epithelioid histiocytes and multinucleated giant cells, together with few OKT4 + helper/inducer and many OKT8 + suppressor/cytotoxic T lymphocytes, some of which expressed Ia-like antigens. B lymphocytes were notably absent from this central part but formed a mantle around each granuloma and expressed predominantly or only IgD at their surfaces. Between the perigranulomatous cuff and the central epithelioid histiocytes, OKT8 + lymphocytes were distributed in a ring-like manner. The results suggest that the underlying immunologic mechanisms in sarcoidosis and tuberculosis are similar. Multinucleated giant cells may function as antigen-processing and -presenting accessory cells, and activated T cells, of either the helper or the suppressor type, may be operative in delayed-type hypersensitivity reactions. The presence of a predominance of IgD or of IgD alone at the surface of the perigranulomatous B lymphocytes may be related to a block in their terminal maturation stage, a defect in accessory cells, the presence of a local population of suppressor cells, or to the importance of IgD in granulomatous disorders.


Assuntos
Granuloma/patologia , Linfadenite/patologia , Sarcoidose/patologia , Tuberculose dos Linfonodos/patologia , Anticorpos Monoclonais/imunologia , Linfócitos B/patologia , Humanos , Linfonodos/patologia , Macrófagos/patologia , Monócitos/patologia , Linfócitos T/patologia
20.
Hum Pathol ; 19(9): 1085-92, 1988 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-3262083

RESUMO

Plasmacytoid T cells (PTCs) are medium-sized cells characterized by abundant rough endoplasmic reticulum. They occur in the thymic-dependent area in human lymph nodes. PTCs are hardly identified in routinely stained sections. We studied their occurrence in 100 reactive lymph nodes with the use of monoclonal antibodies MB2, MT1, LN1, LN2, reactive on paraffin-embedded tissue, and with electron microscopy in nine selected cases. PTCs strongly reactive with MT1 and LN2 were found in 87 of 100 lymph nodes. They were observed in clusters, loose aggregates, and as singular cells. An association between PTCs, postcapillary venules, small T lymphocytes, and interdigitating reticulum cells (IDRCs) was found. Our results indicate that PTCs are normally present in the human lymph node. Their immunophenotype suggests a relationship with a monocyte/macrophage lineage, but does not rule out a T cell origin. If the various distribution patterns represent the morphologic substrate of functional stages of PTCs it can be assumed that PTCs play a role in T cell-mediated immune response.


Assuntos
Linfonodos/citologia , Linfócitos T/ultraestrutura , Adolescente , Adulto , Idoso , Anticorpos Monoclonais , Antígenos de Diferenciação de Linfócitos T/análise , Criança , Pré-Escolar , Feminino , Humanos , Imuno-Histoquímica , Lactente , Linfonodos/imunologia , Linfonodos/ultraestrutura , Masculino , Microscopia Eletrônica , Pessoa de Meia-Idade
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