High-throughput screening for aroma production in food fermentations.

A microtiter plate (MTP) method was developed to screen 1064 unique microorganisms-substrate fermentations for production of 68 target aroma compounds. Based on the number of hits identified by GC-MS, 50 fermentations were repeated at 50-mL scale in flasks. Comparison of GC-MS data showed that scaling up from MTP to flask did not generally result in large differences between the volatile profiles, even with a wide variety of substrates (juice, food slurry and food side-streams) and microorganisms (yeast, bacteria and fungi) used. From the screening results, Lactobacillus plantarum fermentation of chilli pepper was further studied as a high amount of phenols, especially guaiacol and 4-ethylphenol, was produced after fermentation. From HPLC-MS and sensory analysis, capsaicin was shown to be a probable precursor for these phenols and a potential mechanism was proposed. The protocol described herein to screen aroma compounds from fermentation of agri-food products and side streams can support development of clean label flavourful food ingredients.

The Modified Suction-curettage Technique for Minimally Invasive Treatment of Axillary Osmidrosis.

BACKGROUND: Axillary osmidrosis (AO) is a common and nonnegligible disease, the treatment of which is currently lacking a consensus. AIMS: The aim of this study was to introduce a modified suction-assisted technique as a safer and more efficient surgical procedure. METHODS: This retrospective clinical study included 80 patients who recieved a modified suction-curettage procedure (group A) or a subcutaneous gland excision procedure (group B). Intraoperative assessment (endoscopy and pathological biopsy) and postoperative assessment (complications, therapeutic effect, and satisfaction) were performed for both groups. RESULTS: The endoscopy and pathological biopsy results demonstrated that the modified suction-curettage technique could remove the apocrine gland efficiently. Compared with group B, a lower complication rate (long-term, 5.00%; P=0.014, and short-term, 11.10%; P=0.001) and higher patient satisfaction (98.00%, P=0.012) were observed in group A. CONCLUSION: The modified suction-curettage procedure is an effective and safe treatment for axillary osmidrosis.