RESUMO
No specific biomarkers for prognostication or evaluation of tumour load in melanoma have been reported to our knowledge. MicroRNAs (miRNAs) are strongly implicated in oncogenesis and tumour progression, and their circulating forms have been studied as potential biomarkers in oncology. The aim of this prospective study was to identify a melanoma-specific profile of plasma miRNAs. A screening phase, using RNA microarray, was conducted on plasma from 14 patients with metastatic melanoma and 5 healthy subjects. Selected miRNAs were analysed by RTqPCR in 2 independent training and validation cohorts including, respectively, 29 and 31 patients and 16 and 43 control subjects. A profile of 2 miRNAs (miR-1246 and miR-185) significantly associated with metastatic melanoma with a sensitivity of 90.5% and a specificity of 89.1% was identified. This plasma miRNA profile may become an accurate non-invasive biomarker for melanoma.
Assuntos
Biomarcadores Tumorais/sangue , Perfilação da Expressão Gênica , Melanoma/sangue , MicroRNAs/sangue , Neoplasias Cutâneas/sangue , Biomarcadores Tumorais/genética , Estudos de Casos e Controles , Perfilação da Expressão Gênica/métodos , Humanos , Melanoma/genética , Melanoma/secundário , MicroRNAs/genética , Análise de Sequência com Séries de Oligonucleotídeos , Valor Preditivo dos Testes , Prognóstico , Estudos Prospectivos , Reprodutibilidade dos Testes , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Neoplasias Cutâneas/genética , Neoplasias Cutâneas/patologiaRESUMO
In this paper, we propose a multi-group SIR to simulate the spread of COVID-19 in an island context. The multi-group aspect enables us to modelize transmissions of the virus between non-vaccinated individuals within an age group as well as between different age groups. In addition, fuzzy subsets and aggregation operators are used to account for the increased risks associated with age and obesity within these different groups. From a conceptual point of view, the model emphasizes the notion of Hospitalization which is the major stake of this pandemic by replacing the compartment R (Removed) by compartment H (Hospitalization). The experimental results were carried out using medical and demographic data from the archipelago, Guadeloupe (French West Indies) in the Caribbean. These results show that without the respect of barrier gestures, a first wave would concern the elderly then a second the adults and the young people, which conforms to the real data.