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The continuous dissolution and oxidation of active sites in Ru-based electrocatalysts have greatly hindered their practical application in proton exchange membrane water electrolyzers (PEMWE). In this work, we first used density functional theory (DFT) to calculate the dissolution energy of Ru in the 3d transition metal-doped MRuOx (M = Sc, Ti, V, Cr, Mn, Fe, Co, Ni, Cu, and Zn) to evaluate their stability for acidic oxygen evolution reaction (OER) and screen out ZnRuOx as the best candidate. To confirm the theoretical predictions, we experimentally synthesized these MRuOx materials and found that ZnRuOx indeed displays robust acidic OER stability with a negligible decay of η10 after 15 000 CV cycles. Of importance, using ZnRuOx as the anode, the PEMWE can run stably for 120 h at 200 mA cm-2. We also further uncover the stability mechanism of ZnRuOx, i.e., Zn atoms doped in the outside of ZnRuOx nanocrystal would form a "Zn-rich" shell, which effectively shortened average Ru-O bond lengths in ZnRuOx to strengthen the Ru-O interaction and therefore boosted intrinsic stability of ZnRuOx in acidic OER. In short, this work not only provides a new study paradigm of using DFT calculations to guide the experimental synthesis but also offers a proof-of-concept with 3d metal dopants as RuO2 stabilizer as a universal principle to develop high-durability Ru-based catalysts for PEMWE.
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A low-cost, handheld centrifugal microfluidic system for multiplexed visual detection based on recombinase polymerase amplification (RPA) was developed. A concise centrifugal microfluidic chip featuring four reaction units was developed to run multiplexed RPA amplification in parallel. Additionally, a significantly shrunk-size and cost-effective handheld companion device was developed, incorporating heating, optical, rotation, and sensing modules, to perform multiplexed amplification and visual detection. After one-time sample loading, the metered sample was equally distributed into four separate reactors with high-speed centrifugation. Non-contact heating was adopted for isothermal amplification. A tiny DC motor on top of the chip was used to drive steel beads inside reactors for active mixing. Another small DC motor, which was controlled by an elaborate locking strategy based on magnetic sensing, was adopted for centrifugation and positioning. Visual fluorescence detection was optimized from different sides, including material, surface properties, excitation light, and optical filters. With fluorescence intensity-based visual detection, the detection results could be directly observed through the eyes or with a smartphone. As a proof of concept, the handheld device could detect multiple targets, e.g., different genes of African swine fever virus (ASFV) with the comparable LOD (limit of detection) of 75 copies/test compared to the tube-based RPA.
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Técnicas de Amplificação de Ácido Nucleico , Técnicas de Amplificação de Ácido Nucleico/instrumentação , Técnicas de Amplificação de Ácido Nucleico/métodos , Vírus da Febre Suína Africana/isolamento & purificação , Vírus da Febre Suína Africana/genética , Dispositivos Lab-On-A-Chip , Limite de Detecção , Centrifugação/instrumentação , Animais , Smartphone , Técnicas Biossensoriais/instrumentação , Técnicas Biossensoriais/métodos , Técnicas Analíticas Microfluídicas/instrumentação , Técnicas Analíticas Microfluídicas/métodos , Técnicas Analíticas Microfluídicas/economiaRESUMO
The estimation of terrestrial carbon sinks in the Qinghai-Tibet Plateau (QTP) still faces significant uncertainties, and the spatiotemporal dynamics of terrestrial carbon sinks along altitudinal gradients remain unexplored. Moreover, the driving mechanisms of terrestrial carbon sinks at the watershed scale in the QTP continue to be lacking. To address these research gaps, based on multi-source remote sensing data and meteorological data, this study calculated the Net Ecosystem Productivity (NEP) in the QTP from 2000 to 2020 using the Modis NPP-soil respiration model. Through the coefficient of variation (CV), the Mann-Kendall test (MK), and the spatial autocorrelation methods, the spatial distribution pattern and spatiotemporal trends of NEP were investigated. Employing a pixel accumulation method, the variation of NEP along altitudinal gradients was explored. Grey relation analysis, Pearson correlation analysis, and Geographical detector (GD) were used to investigate the driving mechanisms of NEP at the watershed scale. Results showed that: (1) the terrestrial ecosystem in the QTP served as a carbon sink, which produced a total of 2.04 Pg C from 2000 to 2020, and the multi-year average of total carbon sinks was 96.92 Tg C; (2) the spatial distribution of NEP shows a decreasing change from southeast to northwest, and the clustering characteristic of NEP is significant at the watershed scale; (3) the elevation of 4507 m we proposed is likely to be a key threshold for biophysical processes of the terrestrial ecosystems in the QTP; (4) the fluctuation and change trend of carbon sources and carbon sinks show significant differences between the East and West; (5) at the watershed scale, precipitation and temperature play a dominant role in the variation of NEP, while the impact of human activities on NEP variation is weak. Our study aims to address the existing knowledge gaps and provide valuable insights into the management of terrestrial carbon sinks in QTP.
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Sequestro de Carbono , Ecossistema , Tibet , Solo/química , Carbono/análiseRESUMO
Organic materials with switchable dual circularly polarized luminescence (CPL) are highly desired because they can not only directly radiate tunable circularly polarized light themselves but also induce CPL for guests by providing a chiral environment in self-assembled structures or serving as the hosts for energy transfer systems. However, most organic molecules only exhibit single CPL and it remains challenging to develop organic molecules with dual CPL. Herein, novel through-space conjugated chiral foldamers are constructed by attaching two biphenyl arms to the 9,10-positions of phenanthrene, and switchable dual CPL with opposite signs at different emission wavelengths are successfully realized in the foldamers containing high-polarizability substitutes (cyano, methylthiol and methylsulfonyl). The combined experimental and computational results demonstrate that the intramolecular through-space conjugation has significant contributions to stabilizing the folded conformations. Upon photoexcitation in high-polar solvents, strong interactions between the biphenyl arms substituted with cyano, methylthio or methylsulfonyl and the polar environment induce conformation transformation for the foldamers, resulting in two transformable secondary structures of opposite chirality, accounting for the dual CPL with opposite signs. These findings highlight the important influence of the secondary structures on the chiroptical property of the foldamers and pave a new avenue towards efficient and tunable dual CPL materials.
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In this Letter, we propose a flexible bandwidth compression scheme for visible light communication (VLC) systems employing multi-band carrierless amplitude and phase (CAP) modulation. The scheme combines a narrow filtering for every subband at the transmitter and an N-symbol look-up-table (LUT) based maximum likelihood sequence estimation (MLSE) at the receiver. The N-symbol LUT is generated by recording pattern-dependent distortions induced by inter-symbol-interference (ISI), inter-band-interference (IBI), and the other channel effects upon the transmitted signal. The idea is experimentally demonstrated on a 1 m free space optical transmission platform. The results show that the proposed scheme can improve the subband overlap tolerance up to 42% in subband overlapping scenarios, that is, 3 bit/s/Hz, which is the highest spectral efficiency (SE) among the experimented schemes.
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The requirement of carbon emission reduction promotes the continuous implementation and development of low carbon emission mode in typical high-carbon industry aquaculture, especially in the resourceful treatment of terminal waste. However, the previous studies usually focus on a single process or chain, the difference between the long-chain (LC) and automated integration (AI) in the overall environmental impact needs to be quantified and standardised. This paper intends to make a comparative study on two waste treatment biogas projects of aquaculture, a typical industry with high resource consumption and pollution emission in industry and agriculture, and a typical production mode case of aquaculture. The life cycle assessment method is adopted to analyse the environmental impact intensity, identify key link materials, and put forward targeted improvement and optimization schemes. The study found the LC system's comprehensive environmental impact (CEI) is smaller, which is 59.73% less than the AI system. Biogas slurry returning to the field can effectively avoid inorganic fertilizer input. The pretreatment and storage of feces are the key stages. The key substances are NH3 and nitrogen oxides. Suggestions were put forward to optimize the manure management process. In practical pig farms, the process of dry-cleaning manure pre-treatment, automatic fermentation gas production and final biogas slurry filtration drip irrigation should be promoted.
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Biocombustíveis , Carbono , Animais , Fezes , Estágios do Ciclo de Vida , Esterco , SuínosRESUMO
Excessive livestock grazing degrades grasslands ecosystem stability and sustainability by reducing soil organic matter and plant productivity. However, the effects of grazing on soil cellulolytic fungi, an important indicator of the degradation process for soil organic matter, remain less well understood. Using T-RFLP and sequencing methods, we investigated the effects of grazing on the temporal changes of cellulolytic fungal abundance and community structure in dry steppe soils during the growing months from May to September, on the Tibetan Plateau using T-RFLP and sequencing methods. The results demonstrated that the abundance of soil cellulolytic fungi under grazing treatment changed significantly from month to month, and was positively correlated with dissolved organic carbon (DOC) and soil temperature, but negatively correlated with soil pH. Contrastingly, cellulolytic fungal abundance did not change within the fencing treatment (ungrazed conditions). Cellulolytic fungal community structure changed significantly in the growing months in grazed soils, but did not change in fenced soils. Grazing played a key role in determining the community structure of soil cellulolytic fungi by explaining 8.1% of the variation, while pH and DOC explained 4.1% and 4.0%, respectively. Phylogenetically, the cellulolytic fungi were primarily affiliated with Ascomycota (69.65% in relative abundance) and Basidiomycota (30.35%). Therefore, grazing substantially reduced the stability of soil cellulolytic fungal abundance and community structure, as compared with the fencing treatment. Our finding provides a new insight into the responses of organic matter-decomposing microbes for grassland managements.
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Micobioma , Solo , Ecossistema , Solo/química , Microbiologia do Solo , TibetRESUMO
The structural coloration of glass induced by submicron structures is eco-friendly, ink-free, and has profound scientific significance. However, it is difficult to manufacture the submicron structures for glass optics due to the high hardness of glass and the miniature size of the microstructures. In this paper, the diffraction manipulation mechanism of groove shape to structural coloration and optimization theory are studied by establishing the theoretical and simulation mode. Moreover, a newly-developed axial-feed fly-cutting (AFC) technology and the PGM technology are introduced to precisely create the designed submicron V-shape grooves and structural color pattern on a Ni-P mold and then replicating them on a glass surface. Between these two kinds of typical submicron grooves that can be machined by mechanical cutting technology, it is found that the diffraction intensity and efficiency of V-shape grooves are higher than these of jagged-shape grooves, which indicates that V-shape grooves is more suitable to be used for structural coloration with high brightness. The structural color resolution is dramatically increased with the reduction of groove spacing and can be flexibly regulated by AFC, which significantly contributes to the structural coloration manufacturing. Structural pixel segments composed of submicron grooves are arranged row-by-row to form color patterns, and the letters of different colors are fabricated on the mold and transferred to the glass surface. Methods of optical diffraction manipulation, flexible manufacturing of submicron structures, and structural color image construction proposed in this paper for the production of a structural color pattern are beneficial to a wide range of fields.
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OBJECTIVE: Coronavirus disease 2019 (COVID-19) is a major challenge facing the world. Certain guidelines issued by National Health Commission of the People's Repubilic of China recommend intravenous immunoglobulin (IVIG) for adjuvant treatment of COVID-19. However, there is a lack of clinical evidence to support the use of IVIG. METHODS: This single-center retrospective cohort study included all adult patients with laboratory-confirmed severe COVID-19 in the Respiratory and Critical Care Unit of Dabie Mountain Regional Medical Center, China. Patient information, including demographic data, laboratory indicators, the use of glucocorticoids and IVIG, hospital mortality, the application of mechanical ventilation, and the length of hospital stay was collected. The primary outcome was the composite end point, including death and the use of mechanical ventilation. The secondary outcome was the length of hospital stay. RESULTS: Of the 285 patients with confirmed COVID-19, 113 severely ill patients were included in this study. Compared to the non-IVIG group, more patients in the IVIG group reached the composite end point [12 (25.5%) vs 5 (7.6%), P = 0.008] and had longer hospital stay periods [23.0 (19.0-31.0) vs 16.0 (13.8-22.0), P < 0.001]. After adjusting for confounding factors, differences in primary outcomes between the two groups were not statistically significant (P = 0.167), however, patients in the IVIG group had longer hospital stay periods (P = 0.041). CONCLUSION: Adjuvant therapy with IVIG did not improve in-hospital mortality rates or the need for mechanical ventilation in severe COVID-19 patients. Our study does not support the use of immunoglobulin in patients with severe COVID-19 patients.
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Tratamento Farmacológico da COVID-19 , Imunoglobulinas Intravenosas/uso terapêutico , SARS-CoV-2 , Adulto , Idoso , COVID-19/mortalidade , Feminino , Mortalidade Hospitalar , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Índice de Gravidade de DoençaRESUMO
PCR has been widely used in different fields including molecular biology, pathogen detection, medical diagnosis, food detection and etc. However, the difficulty of promoting PCR in on-site point-of-care testing reflects on challenges relative to its speed, convenience, complexity, and even cost. With the emerging state-of-art of microfluidics, rapid PCR can be achieved with more flexible ways in micro-reactors. PCR plays a critical role in the detection of SARS-CoV-2. Under this special background of COVID-19 pandemic, this review focuses on the latest rapid microfluidic PCR. Rapid PCR is concluded in two main features, including the reactor (type, size, material) and the implementation of thermal cycling. Especially, the compromise between speed and sensitivity with microfluidic PCR is explored based on the system ratio of (thermal cycling time)/(reactor size). Representative applications about the detection of pathogens and SARS-CoV-2 viruses based on rapid PCR or other isothermal amplification are discussed as well.
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Nonwoven fiber materials are materials with multifunctional purposes, and are widely used to make masks for preventing the new Coronavirus Disease 2019. Because of the complexity and particularity of their structure, it becomes difficult to model the penetration and flow characteristics of liquid in nonwoven fiber materials. In this paper, a novel seepage time soft sensor model of nonwoven fabric, based on Monte Carlo (MC), integrating extreme learning machine (ELM) (MCELM) is proposed. The Monte Carlo method is used to expand data samples. Then, an ELM method is used to establish the prediction model of the dyeing time of the nonwoven fiber material overlaps with the porous medium, as well as the insertion degree and height of the different quantity of hides. Compared with the back propagation (BP) neural network and radial basis function (RBF) neural network, the results show that the prediction model based on the MCELM method has significant power in terms of accuracy and prediction speed, which is conducive to the precise and rapid manufacture of nonwoven fiber materials in practical applications between liquid seepage characteristics and structural characteristics of porous media. Furthermore, the relationship between the proposed models has certain value for predicting the behavior and use of nonwoven fiber materials with different structural characteristics and related research processes.
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Rare biosphere represents the majority of Earth's biodiversity and performs vital ecological functions, yet little is known about its biogeographical patterns and community assembly processes in terrestrial ecosystems. Herein, we investigated the community composition and phylogeny of rare (relative abundance <0.1%) and abundant (>1%) bacteria in dryland grassland soils on the Tibetan Plateau. Results revealed similar biogeographical patterns of rare and abundant bacteria at both compositional and phylogenetic levels, but rare subcommunity was more heavily influenced by stochasticity (72%) than the abundant (57%). The compositional variation of rare bacteria was less explained by environmental factors (41%) than that of the abundant (80%), while the phylogeny of rare bacteria (36%) was more explained than that of the abundant (29%). The phylogeny of rare bacteria was equally explained by local factors (soil and vegetation) and geospatial distance (11.5% and 11.9% respectively), while that of the abundant was more explained by geospatial distance (22.1%) than local factors (11.3%). Furthermore, a substantially tighter connection between the community phylogeny and composition was observed in rare (R2 = 0.65) than in abundant bacteria (R2 = 0.08). Our study provides novel insights into the assembly processes and biographical patterns of rare and abundant bacteria in dryland soils.
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Bactérias/classificação , Pradaria , Microbiologia do Solo , Bactérias/genética , Biodiversidade , Filogenia , Solo , TibetRESUMO
BACKGROUND: To explore the mechanism of Shenmai injection (SMI) on severe acute pancreatitis (SAP) through heme oxygenase-1 (HO-1) signaling. METHODS: A total of 40 male Sprague-Dawley (SD) rats (220-260 g) were grouped into the following four categories (n = 10): SAP + SMI + Zinc protoporphyrin (ZnPP), SAP + SMI, SAP, and sham surgery groups. ZnPP is a specific inhibitor of HO-1. Four percent of sodium taurocholate (1 mL/kg) was retrogradely injected via the pancreatic duct to induce the SAP model. The SAP group rats received 1.6 mL/kg saline by intravenous injection 30 min after the induction of SAP. The SAP + SMI group rats received 1.6 mL/kg SMI by intravenous injection 30 min after the induction of SAP. The SAP + SMI + ZnPP group rats received an intravenous injection of 1.6 mL/kg SMI and intraperitoneal administration of 30 mg/kg ZnPP 30 min after the SAP induction. Twenty-four hours after the SAP induction, blood samples were collected for the measurement of amylase, lipase, creatinine, myeloperoxidase, interleukin-10 (IL-10), tumor necrosis factor-α (TNF-α), and HO-1 level, while tissue specimens were harvested for the determination of HO-1, TNF-α, and IL-10 mRNA level. Meanwhile, histopathological changes in organs (pancreas, lung, and kidney) were stored. RESULTS: The serum concentration of amylase, lipase, creatinine, and myeloperoxidase was higher in the SAP group than in the SAP + SMI group. Treatment with SMI increased HO-1 and IL-10 level and reduced TNF-α level in serum and tissues compared to the SAP group (P < 0.05). Treatment with SMI abolished the organ-damaging effects of SAP (P < 0.05). Furthermore, suppression of HO-1 expression by ZnPP canceled the aforementioned effects. CONCLUSIONS: SMI confers protection against the SAP-induced systemic inflammatory response and multiple organs damage via HO-1 upregulation.
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Medicamentos de Ervas Chinesas/administração & dosagem , Heme Oxigenase (Desciclizante)/metabolismo , Pâncreas/efeitos dos fármacos , Pancreatite/tratamento farmacológico , Síndrome de Resposta Inflamatória Sistêmica/prevenção & controle , Amilases/sangue , Animais , Modelos Animais de Doenças , Combinação de Medicamentos , Humanos , Lipase/sangue , Masculino , Pâncreas/imunologia , Pâncreas/patologia , Pancreatite/sangue , Pancreatite/complicações , Pancreatite/diagnóstico , Peroxidase/sangue , Ratos , Índice de Gravidade de Doença , Síndrome de Resposta Inflamatória Sistêmica/sangue , Síndrome de Resposta Inflamatória Sistêmica/diagnóstico , Síndrome de Resposta Inflamatória Sistêmica/etiologia , Regulação para Cima/efeitos dos fármacosRESUMO
Optical cell manipulation has become increasingly valuable in cell-based assays. In this paper, we demonstrate the translational and rotational manipulation of filamentous cells using multiple cooperative microrobots automatically driven by holographic optical tweezers. The photodamage of the cells due to direct irradiation of the laser beam can be effectively avoided. The proposed method will enable fruitful biomedical applications where precise cell manipulation and less photodamage are required.
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Anabaena/citologia , Micromanipulação/instrumentação , Pinças Ópticas , Robótica/instrumentação , Rotação , Holografia , Imageamento TridimensionalRESUMO
BACKGROUND: Heme oxygenase-1 (HO-1) is an inducible defense gene which plays a significant role in inflammation. HO-1 protects cells and tissues through the mechanism of anti-oxidation, maintaining microcirculation and anti-inflammation. The aim of the current study is to investigate the role of HO-1 on systemic inflammatory response in severe acute pancreatitis (SAP). METHODS: Forty male Sprague-Dawley (SD) rats were randomly assigned into four groups: control group (n = 10); SAP group (n = 10), SAP model was induced by retrograde injection of 3% sodium taurocholate through pancreatic duct; HO-1 stimulation group (n = 10), SD rats were injected 75 µg/kg hemin intraperitoneally 30 min after induction of SAP; HO-1 inhibition group (n = 10), SD rats were injected 20 µg/kg Zinc porphyrin (Zn-PP) intraperitoneally 30 min after induction of SAP. After 24 h of SAP establishment, tissues were collected for HO-1, tumor necrosis factor-α (TNF-α) and interleukin-10 (IL-10) mRNA expression, and blood samples were collected for cytokines and biochemical measurements. Meanwhile, the histopathological changes of pancreas and liver tissues were observed. RESULTS: The expression of HO-1 mRNA and protein were significantly induced by SAP in rat pancreas and liver. Hemin treatment significantly decreased oxidative stress and TNF-α in plasma and tissues, while the IL-10 was significantly increased. Pancreas and liver injury induced by SAP was markedly attenuated by Hemin treatment. Moreover, inhibition of HO-1 expression by Zn-PP administration aggravated the injury caused by SAP. CONCLUSIONS: Induction of HO-1 in early SAP may modulate systemic inflammatory response and prevent pancreas and nearby organs such as liver injury through inhibition of TNF-α and augmentation of IL-10.
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Heme Oxigenase-1/farmacologia , Interleucina-10/metabolismo , Pancreatite/tratamento farmacológico , Substâncias Protetoras/farmacologia , Fator de Necrose Tumoral alfa/antagonistas & inibidores , Doença Aguda , Animais , Modelos Animais de Doenças , Hemina/farmacologia , Fígado/metabolismo , Masculino , Metaloporfirinas/farmacologia , Estresse Oxidativo/efeitos dos fármacos , Pâncreas/metabolismo , Pancreatite/induzido quimicamente , Ratos , Ratos Sprague-Dawley , Ácido TaurocólicoRESUMO
Unraveling elevational diversity patterns of plants and animals has long been attracting scientific interests. However, whether soil microorganisms exhibit similar elevational patterns remains largely less explored, especially for functional microbial communities, such as ammonia oxidizers. Here, we investigated the diversity and distribution pattern of ammonia-oxidizing archaea (AOA) in meadow soils along an elevation gradient from 4400 m to the grassline at 5100 m on the Tibetan Plateau using terminal restriction fragment length polymorphism (T-RFLP) and sequencing methods by targeting amoA gene. Increasing elevations led to lower soil temperature and pH, but higher nutrients and water content. The results showed that AOA diversity and evenness monotonically increased with elevation, while richness was relatively stable. The increase of diversity and evenness was attributed to the growth inhibition of warm-adapted AOA phylotypes by lower temperature and the growth facilitation of cold-adapted AOA phylotypes by richer nutrients at higher elevations. Low temperature thus played an important role in the AOA growth and niche separation. The AOA community variation was explained by the combined effect of all soil properties (32.6%), and 8.1% of the total variation was individually explained by soil pH. The total AOA abundance decreased, whereas soil potential nitrification rate (PNR) increased with increasing elevations. Soil PNR positively correlated with the abundance of cold-adapted AOA phylotypes. Our findings suggest that low temperature plays an important role in AOA elevational diversity pattern and niche separation, rising the negative effects of warming on AOA diversity and soil nitrification process in the Tibetan region.
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Amônia/metabolismo , Archaea/metabolismo , Pradaria , Nitrificação , Oxirredução , Polimorfismo de Fragmento de Restrição , Solo , TibetRESUMO
We propose a new type of integral imaging-based large-scale full-color three-dimensional (3-D) display of holographic data based on direct ray-optical conversion of holographic data into elemental images (EIs). In the proposed system, a 3-D scene is modeled as a collection of depth-sliced object images (DOIs), and three-color hologram patterns for that scene are generated by interfering each color DOI with a reference beam, and summing them all based on Fresnel convolution integrals. From these hologram patterns, full-color DOIs are reconstructed, and converted into EIs using a ray mapping-based direct pickup process. These EIs are then optically reconstructed to be a full-color 3-D scene with perspectives on the depth-priority integral imaging (DPII)-based 3-D display system employing a large-scale LCD panel. Experiments with a test video confirm the feasibility of the proposed system in the practical application fields of large-scale holographic 3-D displays.
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We propose a new approach for optical refocusing of three-dimensional (3-D) objects on their real depth without a pickup-range limitation based on subdivided-elemental image arrays (sub-EIAs) and local periodic δ-function arrays (L-PDFAs). The captured EIA from the 3-D objects locating out of the pickup-range, is divided into a number of sub-EIAs depending on the object distance from the lens array. Then, by convolving these sub-EIAs with each L-PDFA whose spatial period corresponds to the specific object's depth, as well as whose size is matched to that of the sub-EIA, arrays of spatially-filtered sub-EIAs (SF-sub-EIAs) for each object depth can be uniquely extracted. From these arrays of SF-sub-EIAs, 3-D objects can be optically reconstructed to be refocused on their real depth. Operational principle of the proposed method is analyzed based on ray-optics. In addition, to confirm the feasibility of the proposed method in the practical application, experiments with test objects are carried out and the results are comparatively discussed with those of the conventional method.
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A new three-directional motion compensation-based novel-look-up-table (3DMC-NLUT) based on its shift-invariance and thin-lens properties, is proposed for video hologram generation of three-dimensional (3-D) objects moving with large depth variations in space. The input 3-D video frames are grouped into a set of eight in sequence, where the first and remaining seven frames in each set become the reference frame (RF) and general frames (GFs), respectively. Hence, each 3-D video frame is segmented into a set of depth-sliced object images (DOIs). Then x, y, and z-directional motion vectors are estimated from blocks and DOIs between the RF and each of the GFs, respectively. With these motion vectors, object motions in space are compensated. Then, only the difference images between the 3-directionally motion-compensated RF and each of the GFs are applied to the NLUT for hologram calculation. Experimental results reveal that the average number of calculated object points and the average calculation time of the proposed method have been reduced compared to those of the conventional NLUT, TR-NLUT and MPEG-NLUT by 38.14%, 69.48%, and 67.41% and 35.30%, 66.39%, and 64.46%, respectively.
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A new robust MPEG-based novel look-up table (MPEG-NLUT) is proposed for accelerated computation of video holograms of fast-moving three-dimensional (3-D) objects in space. Here, the input 3-D video frames are sequentially grouped into sets of four, in which the first and remaining three frames in each set become the reference (RF) and general frames (GFs). Then, the frame images are divided into blocks, from which motion vectors are estimated between the RF and each of the GFs, and with these estimated motion vectors, object motions in all blocks are compensated. Subsequently, only the difference images between the motion-compensated RF and each of the GFs are applied to the NLUT for CGH calculation based on its unique property of shift-invariance. Experiments with three types of test 3-D video scenarios confirm that the average number of calculated object points and the average calculation time of the proposed method, have found to be reduced down to 27.34%, 55.46%, 45.70% and 19.88%, 44.98%, 30.72%, respectively compared to those of the conventional NLUT, temporal redundancy-based NLUT (TR-NLUT) and motion compensation-based NLUT (MC-NLUT) methods.