Compost and mycorrhizae application as a technique to alleviate Cd and Zn stress in Medicago sativa.

Soil pollution by heavy metals, in the last decades, has become a worldwide major concern for which finding a solution is becoming more important to conserve soil for future generations. This study used an ecotoxicology approach to evaluate the effectiveness of compost and arbuscular mycorrhizal fungus (AMF) and their combination on Medicago sativa performance grown under Zn and Cd stress. At 600 mg/kg of Cd and Zn, a reduction of mycorrhization frequency by 3.6- and 2-fold, respectively, was observed without applying compost. The effect of AMF-Compost combination on alfalfa biomass production was enhanced in the absence and the presence of heavy metals. An improvement of relative water content by 1.7- and 1.5-fold was recorded in case AMF-Compost plant treatments grown under 600 mg/kg of Cd and Zn, respectively. The application of AMF-compost enhanced the stomatal conductance and total chlorophyll in alfalfa plants. Sugar contents were significantly increased in mycorrhized and treated plants with compost compared to the control, regardless of the applied Cd or Zn dose. Phenol content was significantly increased in plants amended with compost alone and treated by Cd. Regarding Cd and Zn accumulation, AMF-compost combination reduced the content of heavy metals accumulated in M. sativa.

Use of native plants and their associated bacteria rhizobiomes to remediate-restore Draa Sfar and Kettara mining sites, Morocco.

Soil and mine tailings are unreceptive to plant growth representing an imminent threat to the environment and resource sustainability. Using indigenous plants and their associated rhizobacteria to restore mining sites would be an eco-friendly solution to mitigate soil-metal toxicity. Soil prospection from Draa Sfar and Kettara mining sites in Morocco was carried out during different seasons for native plant sampling and rhizobacteria screening. The sites have been colonized by fifteen tolerant plant species having different capacities to accumulate Cu, Zn, and P in their shoots/root systems. In Draa Sfar mine, Suaeda vera J.F. Gmel., Sarcocornia fruticosa (L.) A.J. Scott., and Frankenia corymbosa Desf. accumulated mainly Cu (more than 90 mg kg-1), Atriplex halimus L. accumulated Zn (mg kg-1), and Frankenia corymbosa Desf. accumulated Pb (14 mg kg-1). As for Kettara mine, Aizoon canariense L. mainly accumulated Zn (270 mg kg-1), whereas Forsskalea tenacissima L. was the best shoot Cu accumulator with up to 50 mg kg-1, whereas Cu accumulation in roots was 21 mg kg-1. The bacterial screening revealed the strains' abilities to tolerate heavy metals up to 50 mg kg-1 Cu, 250 mg kg-1 Pb, and 150 mg kg-1 Zn. Isolated strains belonged mainly to Bacillaceae (73.33%) and Pseudomonadaceae (10%) and expressed different plant growth-promoting traits, alongside their antifungal activity. Results from this study will provide an insight into the ability of native plants and their associated rhizobacteria to serve as a basis for remediation-restoration strategies.

Resistance to and accumulation of heavy metals by actinobacteria isolated from abandoned mining areas.

Accumulation of high concentrations of heavy metals in environments can cause many human health risks and serious ecological problems. Nowadays, bioremediation using microorganisms is receiving much attention due to their good performance. The aim of this work is to investigate heavy metals resistance and bioaccumulation potential of actinobacteria strains isolated from some abandoned mining areas. Analysis of mining residues revealed that high concentration of zinc "Zn" was recorded in Sidi Bouatman, Arbar, and Bir Nhass mining residues. The highest concentration of lead "Pb" was found in Sidi Bouatman. Copper "Cu," cadmium "Cd," and chromium "Cr" were found with moderate and low concentrations. The resistance of 59 isolated actinobacteria to the five heavy metals was also determined. Using molecular identification 16S rRNA, these 27 isolates were found to belong to Streptomyces and Amycolatopsis genera. The results showed different levels of heavy metal resistance; the minimum inhibitory concentration (MIC) recorded was 0.55 for Pb, 0.15 for Cr, and 0.10 mg·mL(-1) for both Zn and Cu. Chemical precipitation assay of heavy metals using hydrogen sulfide technic (H2S) revealed that only 27 isolates have a strong ability to accumulate Pb (up to 600 mg of Pb per g of biomass for Streptomyces sp. BN3).