Effects of El Niño-Southern Oscillation on human visceral leishmaniasis in the Brazilian State of Mato Grosso do Sul.

BACKGROUND: Leishmaniases are considered a major public health problem in South America, specifically in Brazil. Moreover, the transmission and epidemiology of leishmaniasis are possibly associated with climatic and environmental variations. OBJECTIVE: This study aimed to assess the association between the extreme climatic phenomenon El Niño-Southern Oscillation (ENSO), the maximum and minimum variations of temperature, precipitation, and soil moisture and the incidence of visceral leishmaniasis (VL) in Mato Grosso do Sul (MS), Brazil, from 2002 to 2015. METHODS: The Niño 3.4 index was used for the ENSO variation. The other climatic data were obtained from the climatic tool TerraClimate. Records regarding VL were obtained from the Notification of Injury Information System. FINDINGS: From 2002 to 2015, there were 3,137 cases of VL recorded in MS. The annual incidence of the disease was negatively associated with the ENSO index and soil moisture in MS. The VL incidence increased during the negative phase of ENSO and decreased during the positive phase. MAIN CONCLUSIONS: The results demonstrated that the interannual cycles of incidence of human VL are significantly associated with the occurrence of the ENSO phenomenon and its phases, El Niño and La Niña.

SAND FLIES (DIPTERA: PSYCHODIDAE) IN AN ENDEMIC AREA OF LEISHMANIASIS IN AQUIDAUANA MUNICIPALITY, PANTANAL OF MATO GROSSO DO SUL , BRAZIL.

The Aquidauana municipality is considered an endemic area of leishmaniasis and an important tourist site in Mato Grosso do Sul State. The aim of this study was to investigate the sand fly fauna in the city of Aquidauana. Captures were carried out twice a month, from April 2012 to March 2014 with automatic light traps and active aspiration, in the peridomicile and domicile of six residences. A total of 9,338 specimens were collected, 3,179 and 6,159 using light traps and active aspiration, respectively. The fauna consisted of: Brumptomyia brumpti, Evandromyia aldafalcaoae, Ev. evandroi, Ev. lenti, Ev. orcyi, Ev. sallesi, Ev. termitophila, Ev. walkeri, Lutzomyia longipalpis and Psathyromyia bigeniculata. The most abundant species captured was Lutzomyia longipalpis, present in all the ecotopes, predominantly in peridomicile areas, and mainly males. Leishmania DNA was not detected in the insects. It was observed the abundance of the sand fly fauna in the region, as well as the high frequency of Lu. longipalpis, the main vector of L. infantum. The results of this study show the need to increase the monitoring and more effective control measures. It is noteworthy that the studied region presents several activities related to tourism and recreation, increasing the risk of transmission of leishmaniasis to this particular human population.

Renal histopathological findings in dogs with visceral leishmaniasis.

Visceral leishmaniasis affects various organs including the kidneys; which can lead to renal failure and death. In order to verify this renal involvement, material was evaluated from 100 dogs naturally infected and with serological diagnosis of canine visceral leishmaniasis (CVL). Inflammatory changes were present in 25.3% of the tubules, in 67.0% of interstitium and in 52.0% of glomeruli. There was no significant difference (p > 0.05) between the presence of glomerulonephritis in symptomatic and oligosymptomatic dogs. The membranous and membranoproliferative glomerulonephritis were the most frequent, both with 18.0% frequency, followed by focal segmental glomerulosclerosis with 14.0%. Changes such as cylindruria, tubular and fibrosis hypertrophy, periglomerular inflammatory infiltrate, and multifocal and diffuse peritubular inflammatory infiltrate were observed. The findings are consistent with those of other authors indicating that renal involvement is common in CVL and the standards of membranous and membranoploriferative glomerulonephritis, as well as the tubulointerstitial involvement, are frequent.

Renal histopathllogical findings in dogs with visceral leishmaniasis / Achados histopatológicos renais em cães com leishmaniose visceral

Visceral leishmaniasis affects various organs including the kidneys; which can lead to renal failure and death. In order to verify this renal involvement, material was evaluated from 100 dogs naturally infected and with serological diagnosis of canine visceral leishmaniasis (CVL). Inflammatory changes were present in 25.3% of the tubules, in 67.0% of interstitium and in 52.0% of glomeruli. There was no significant difference (p > 0.05) between the presence of glomerulonephritis in symptomatic and oligosymptomatic dogs. The membranous and membranoproliferative glomerulonephritis were the most frequent, both with 18.0% frequency, followed by focal segmental glomerulosclerosis with 14.0%. Changes such as cylindruria, tubular and fibrosis hypertrophy, periglomerular inflammatory infiltrate, and multifocal and diffuse peritubular inflammatory infiltrate were observed. The findings are consistent with those of other authors indicating that renal involvement is common in CVL and the standards of membranous and membranoploriferative glomerulonephritis, as well as the tubulointerstitial involvement, are frequent.

A leishmaniose visceral acomete vários órgãos entre eles os rins; o que pode levar a insuficiência renal e a morte. Com o objetivo de verificar este acometimento renal foram avaliados materiais de 100 cães naturalmente infectados e com diagnósticos sorológicos de leishmaniose visceral canina - LVC. As alterações inflamatórias estavam presentes em 25,3% dos túbulos, em 67,0% do interstício e em 52,0% dos glomérulos. Não houve diferença significativa (p > 0,05) entre a presença de glomerulonefrite em cães sintomáticos e oligossintomáticos. As glomerulonefrites membranosa e membrano proliferativa foram as mais freqüentes, ambas com 18,0% de freqüência seguidas da glomeruloesclerose segmentar e focal com 14,0%. Foram observadas alterações como cilindrúria, hipertrofia tubular e fibrose e infiltrados inflamatórios periglomerulares e peritubulares multifocais e difusos. Os achados concordam com os de outros autores indicando que o acometimento renal é comum na LVC e que os padrões de glomerulonefrites membranoploriferativa e membranosa; assim como o acometimento tubulointersticial são freqüentes.

Inquérito soroepidemiológico para toxoplasmose e avaliação dos condicionantes para sua transmissão em universitários de Campo Grande, Mato Grosso do Sul / Seroepidemiological survey of toxoplasmosis and evaluation of theconditioning factors for its transmission in undergraduate students fromCampo Grande, Mato Grosso do Sul State, Brazil

Objetivos: avaliar, por meio de inquérito soroepidemiológico, a ocorrência da infecção pelo Toxoplasma gondii em universitários dos cursos de Enfermagem e Ciências Biológicas da Universidade Anhanguera ? UNIDERP, Campo Grande, Mato Grosso do Sul. Métodos: as amostras de soro foram submetidas ao teste de ELISA de fase sólida para determinação dos anticorpos específicos IgM e IgG contra Toxoplasma gondii. Os dados foram correlacionados a fatores sociodemográficos e hábitos que poderiam ter influenciado na transmissão da infecção. Resultados: entre os 100 universitários estudados, 39% (39/100) apresentaram-se IgG reagentes para toxoplasmose (intervalo de confiança [IC] 95%: 29,4%-48,6%) e nenhum IgM reagente. Observou-se associação estatisticamente significativa entre a presença de gatos em domicílio e a positividade para toxoplasmose. Entre os acadêmicos soro reagentes, 72,97%(IC 95%: 58,7%-87,3%) declararam possuir ou ter possuído gatos em domicílio, enquanto que entre os soronegativos para toxoplasmose, 50% (IC 95%: 36,9%-63,1%) relatavam possuir ou ter possuído gatos. Com relação aos fatores de risco alimentares (ingestão de carne crua/mal cozida, legumes crus, leite cru e ovo cru), não foi detectada associação com a infecção por Toxoplasma gondii. A frequência de indivíduos soro reagentes foi significativamente maior na faixa etária acima dos 25 anos (p=0,01). Conclusões: a ocorrência de anticorpos anti-Toxoplasma gondii foi relativamente baixa em relação à prevalência média encontrada em diferentes amostras populacionais do Brasil. O fator de risco identificado como provável responsável pela infecção foi a presença de gatos no domicílio. A prevalência de soropositividade para toxoplasmose foi maior na faixa etária acima dos 25 anos.

Aims: To evaluate, by means of a seroepidemiological survey, the occurrence of Toxoplasma gondii infection in undergraduate students of Nursing and Biological Sciences from the Anhanguera ? UNIDERP University, in Campo Grande, Mato Grosso do Sul State. Methods: Serum samples were tested with solid phase ELISA for determination of specific IgM and IgG antibodies against Toxoplasma gondii. The data were correlated to sociodemographic factors and habits that might have influenced the transmission of infection. Results: Among the 100 students studied, 39% (39/100) were IgG reagent for toxoplasmosis (confidence interval [CI) 95%: 29.4%-48.6%) and none were IgM reagent. A statistically significant association between the presence of cats at home and positivity for toxoplasmosis was found. Among serum reagent academics, 72.97% (95% CI: 58.7%-87.3%) reported owning or having owned cats in the home, while among the seronegative for toxoplasmosis, 50% (CI 95%: 36.9%-63.1%) reported owning or having owned cats. With respect to alimentary risk factors (eating of raw/undercooked meat, raw vegetables, raw milk and raw egg), no association was found with Toxoplasma gondii infection. The frequency of serum reactive individuals was significantly higher in the age group above 25 years (p=0.01). Conclusions: The occurrence of anti-Toxoplasma gondii specific antibodies was relatively low when compared to the average prevalence found in different population samples from Brazil. Presence of cats at home was the risk factor identified as likely responsible for the infection. The prevalence of seropositivity for toxoplasmosis was higher in the age group above 25 years.