Do Methodological Differences in Experiments with Stream Shredders Imply Variability in Outputs? A Microcosm Approach.

Experiments are useful scientific tools for testing hypotheses by manipulating variables of interest while controlling for other factors that can bias or confuse the results and their interpretation. To ensures accuracy and reproducibility, experiments must have transparent and repeatable methodologies. Due to the importance of shredder invertebrates in organic matter processing, carbon cycling, and nutrient cycling, we tested experimentally the effect of different methodological approaches in microcosm experiments on the consumption and survival of shredders. We found that the shredder species, the presence or absence of the case, and the use or non-use of air-pumps in the microcosms did not affect shredder performance (i.e., consumption and survival). Furthermore, the type of water (stream or bottled) did not affect shredder performance. On the other hand, the amount of light had a negative effect on shredder performance, with constant light (i.e., 24 h) reducing shredder consumption and survival. Our results demonstrate that the use of different methodologies does not always result in changes in outcomes, thus ensuring comparability. However, luminosity is a critical factor that deserves attention when conducting microcosm experiments. Our findings provide valuable insights that can assist researchers in designing experiments with shredders from neotropical streams and conducting systematic reviews and meta-analyses.

Do microplastics and climate change negatively affect shredder invertebrates from an amazon stream? An ecosystem functioning perspective.

Pollution and climate change are among the main threats to the biodiversity of freshwater ecosystems in the 21st century. We experimentally tested the effects of microplastic and climate change (i.e., increase in temperature and CO2) on the survival and consumption by an Amazonian-stream shredder invertebrate. We tested three hypotheses. (1) Increased microplastic concentrations and climate change reduce shredder survival. We assumed that the combined stressors would increase toxic stress. (2) Increased concentrations of microplastics have negative effects on shredder food consumption. We assumed that blockage of the digestive tract by microplastics would lead to reduced ability to digest food. In addition, increased temperature and CO2 would lead to an increase in metabolic cost and reduced consumption. (3) The interaction between microplastics and climate change have greater negative effects on survival and consumption than either alone. We combined different concentrations of microplastic and climate change scenarios to simulate in real-time increases in temperature and CO2 forecast for 2100 for Amazonia. We found that both stressors had lethal effects, increasing mortality risk, but there was no interaction effect. Shredder consumption was negatively affected only by climate change. The interaction of microplastics and climate change on shredder consumption was dose-dependent and more intense in the extreme climate scenario, leading to reduced consumption. Our results indicate that microplastic and climate change may have strong effects on the consumption and/or survival of insect shredders in Amazonian streams. In addition, microplastic and climate change effects may affect not only populations but also ecosystem functioning (e.g., nutrient cycling). Integrative approaches to better understand and mitigate the effects of both stressors are necessary because plastic pollution and climate change co-occur in environments.

First occurrence of Anacroneuria singularis Righi-Cavallaro Lecci, 2010 (Plecoptera: Perlidae) in Rondônia, western Amazonia, Brazil.

Anacroneuria singularis is newly reported from the northern Brazilian state of Rondônia in the western Amazonia. Records for this species are also reported for the central-western and southeastern regions of Brazil. Knowledge of the distribution of a species is fundamental for allowing better conservation planning, especially the identification of nymphs which can be used to evaluate water quality of Amazonian streams.

Phlebotomines (Diptera: Psychodidae) in a Hydroelectric System Affected Area from Northern Amazonian Brazil: Further Insights into the Effects of Environmental Changes on Vector Ecology.

During 2012-2015, an entomological survey was conducted as part of a phlebotomine (Diptera: Psychodidae) monitoring program in an area influenced by the Santo Antônio do Jari hydroelectric system (Amapá State, Brazil). The purpose was to study aspects of Amazon/Guianan American cutaneous leishmaniasis (ACL) vectors subjected to stresses by anthropogenic environmental changes. For sampling, CDC light traps were positioned 0.5, 1, and 20 m above ground at five capture locations along the Jari River Basin. Fluctuations in phlebotomine numbers were analyzed to determine any correlation with rainfall, dam waterlogging, and/or ACL cases, from May 2012 to March 2015. We captured 2,800 individuals, and among 45 species identified, Bichromomyia flaviscutellata, Nyssomyia umbratilis, and Psychodopygus squamiventris s.l. were determined to be the main putative vectors, based on current knowledge of the Amazon/Guianan ACL scenario. Rainfall, but not complete flooding, was relatively correlated with phlebotomine fluctuation, mainly observed for Ps. squamiventris s.l., as were ACL cases with Ny. umbratilis. Behavioral changes were observed in the unexpected high frequency of Bi. flaviscutellata among CDC captures and the noncanopy dominance of Ny. umbratilis, possibly attributable to environmental stress in the sampled ecotopes. Continuous entomological surveillance is necessary to monitor the outcomes of these findings.