Resilience of DNA chains to molecular fracture after PCR heating cycles and implications on PCR reliability.

Soon after its introduction in 1987, polymerase chain reaction (PCR) has become a technique widely employed in diagnostic medical devices and forensic science with the intention of amplifying genetic information. PCR prescribes that each of its cycles must include a heating subprocess at 95 °C or more (denominated DNA denaturation and provided for allowing a claimed orderly separation of the two complementary nucleotides strands), which can produce significant damage to DNA, caused by high-speed collisions with surrounding molecules. Since such disruption should be prevented in order to reliably employ PCR, a study of the mechanics of such loss of structural integrity is herein presented, preceded by a review of the fundamental literature which has elucidated the effects of molecular agitation on DNA fragmentation. The main conclusion of this retrospective survey is that the body of examined theoretical and experimental evidence consistently and redundantly confirms scarce resilience and significant loss of structural integrity when DNA is heated at temperatures above 90 °C, even for 1 minute. Such conclusion contradicts the claimed paradigm of PCR fidelity and raises the concern that, at least for long sequences, if PCR can amplify some information, such amplified information may be unreliable for diagnostic or forensic applications, since it originates from sequences of nucleotides subjected to random fragmentation and reaggregation. Such a low-reliability scenario should be preventively considered in the various fields where DNA amplification methodologies are employed which provide for high-temperature heating under conditions equal to or similar to those prescribed by the PCR protocols reviewed in this study.

Developing and Testing High-Performance SHM Sensors Mounting Low-Noise MEMS Accelerometers.

Recently, there has been increased interest in adopting novel sensing technologies for continuously monitoring structural systems. In this respect, micro-electrical mechanical system (MEMS) sensors are widely used in several applications, including structural health monitoring (SHM), in which accelerometric samples are acquired to perform modal analysis. Thanks to their significantly lower cost, ease of installation in the structure, and lower power consumption, they enable extensive, pervasive, and battery-less monitoring systems. This paper presents an innovative high-performance device for SHM applications, based on a low-noise triaxial MEMS accelerometer, providing a guideline and insightful results about the opportunities and capabilities of these devices. Sensor nodes have been designed, developed, and calibrated to meet structural vibration monitoring and modal identification requirements. These components include a protocol for reliable command dissemination through network and data collection, and improvements to software components for data pipelining, jitter control, and high-frequency sampling. Devices were tested in the lab using shaker excitation. Results demonstrate that MEMS-based accelerometers are a feasible solution to replace expensive piezo-based accelerometers. Deploying MEMS is promising to minimize sensor node energy consumption. Time and frequency domain analyses show that MEMS can correctly detect modal frequencies, which are useful parameters for damage detection. The acquired data from the test bed were used to examine the functioning of the network, data transmission, and data quality. The proposed architecture has been successfully deployed in a real case study to monitor the structural health of the Marcus Aurelius Exedra Hall within the Capitoline Museum of Rome. The performance robustness was demonstrated, and the results showed that the wired sensor network provides dense and accurate vibration data for structural continuous monitoring.

What Can 5G Do for Public Safety? Structural Health Monitoring and Earthquake Early Warning Scenarios.

The 5th generation of mobile networks has come to the market bringing the promise of disruptive performances as low latency, availability and reliability, imposing the development of the so-called "killer applications". This contribution presents a 5G use case in the context of Structural Health Monitoring which guarantees an unprecedented level of reliability when exploited for public safety purposes as Earthquake Early Warning. The interest on this topic is at first justified through a deep market analysis, and subsequently declined in terms of public safety benefits. A specific sensor board, guaranteeing real-time processing and 5G connectivity, is presented as the foundation on which the architecture of the network is designed and developed. Advantages of 5G-enabled urban safety are then discussed and proven in the experimentation results, showing that the proposed architecture guarantees lower latency delays and overcome the impairments of cloud solutions especially in terms of delays variability.

Calvarial tuberculosis as the initial presentation of AIDS.

A rare case of a young immunocompromised patient with calvarial tuberculosis as the first symptom of human immunodeficiency virus-1 positivity is presented and the pertinent literature is reviewed.

Molecular epidemiology and characteristics of Corynebacterium diphtheriae and Corynebacterium ulcerans strains isolated in Italy during the 1990s.

Five cases of diphtheria were reported in Italy between January 1990 and June 2001. Three cases were confirmed microbiologically by the isolation of toxigenic Corynebacterium diphtheriae (two cases) and Corynebacterium ulcerans (one case). Over the same period, 11 cases of non-toxigenic C. diphtheriae infection were reported to the Italian Public Health Institute, from which the causative organism was isolated from a skin infection in one case and from the throat in the other ten. Seven of the throat isolates were associated with fever, severe pharyngitis and tonsillitis and were all biotype gravis. Because there are no standardized breakpoints, the antimicrobial sensitivities of C. diphtheriae were determined in accordance with the National Committee for Clinical Laboratory Standards guidelines for Streptococcus spp. other than Streptococcus pneumoniae. MICs for penicillin ranged between 0.125 and 0.250 mg l(-1) and 7 out of 11 strains had a minimal bactericidal concentration (MBC)/MIC ratio >or= 32. All strains were sensitive to clindamycin (MIC