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J Sci Food Agric ; 99(3): 1250-1257, 2019 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-30066395

RESUMO

BACKGROUND: The aim of this work was to determine the most favorable conditions for the production of xylooligosaccharides (XOS) from Brazilian Syrah grape pomace. Chemical processes were performed using a rotatable central composite design where the concentration of sulfuric acid or sodium hydroxide and the grape pomace flour/solvent mass ratio were the dependent variables. Enzymatic production was also evaluated using xylanase produced by Aspergillus niger 3T5B8 and Viscozyme® enzymatic commercial cocktail. RESULTS: Chemical extraction allowed to recover 21.8-74.6% and 5.2-96.3% of total XOS for acidic and alkaline processes respectively. Enzymatic production extracted up to 88.68 ± 0.12% of total XOS using xylanase and up to 84.09 ± 2.40% with Viscozyme® . CONCLUSION: The present study demonstrated different feasible methods to produce high-added-value molecules, i.e. XOS, from Syrah grape pomace flour, valorizing this major by-product. The use of enzymatic cocktails demonstrated to be an alternative to the conventional methods, allowing to obtain an eco-friendly and sustainable grape pomace extract. © 2018 Society of Chemical Industry.


Assuntos
Endo-1,4-beta-Xilanases/química , Farinha/análise , Proteínas Fúngicas/química , Glucuronatos/química , Oligossacarídeos/química , Extratos Vegetais/química , Vitis/química , Resíduos/análise , Aspergillus niger/enzimologia , Biocatálise , Brasil , Glucuronatos/isolamento & purificação , Oligossacarídeos/isolamento & purificação , Extratos Vegetais/isolamento & purificação
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