Metformin Preserves VE-Cadherin in Choroid Plexus and Attenuates Hydrocephalus via VEGF/VEGFR2/p-Src in an Intraventricular Hemorrhage Rat Model.

Hydrocephalus induced by intraventricular hemorrhage (IVH) is associated with unfavorable prognosis. The increased permeability of choroid plexus and breakdown of the blood-brain barrier (BBB) was reported as a prominent mechanism of IVH-induced hydrocephalus, and vascular endothelial-cadherin (VE-cadherin) was demonstrated to be relevant. Metformin was reported to protect endothelial junction and preserve permeability widely; however, its role in hydrocephalus remains unclear. In this study, the decreased expression of VE-cadherin in the choroid plexus, accompanied with ventricle dilation, was investigated in an IVH rat model induced by intraventricular injection of autologous blood. Metformin treatment ameliorated hydrocephalus and upregulated VE-cadherin expression in choroid plexus meanwhile. We then observed that the internalization of VE-cadherin caused by the activation of vascular endothelial growth factor (VEGF) signaling after IVH was related to the occurrence of hydrocephalus, whereas it can be reversed by metformin treatment. Restraining VEGF signaling by antagonizing VEGFR2 or inhibiting Src phosphorylation increased the expression of VE-cadherin and decreased the severity of hydrocephalus after IVH. Our study demonstrated that the internalization of VE-cadherin via the activation of VEGF signaling may contribute to IVH-induced hydrocephalus, and metformin may be a potential protector via suppressing this pathway.

Delayed Minocycline Treatment Ameliorates Hydrocephalus Development and Choroid Plexus Inflammation in Spontaneously Hypertensive Rats.

Hydrocephalus is a complicated disorder that affects both adult and pediatric populations. The mechanism of hydrocephalus development, especially when there is no mass lesion present causing an obstructive, is poorly understood. Prior studies have demonstrated that spontaneously hypertensive rats (SHRs) develop hydrocephalus by week 7, which was attenuated with minocycline. The aim of this study was to determine sex differences in hydrocephalus development and to examine the effect of minocycline administration after hydrocephalus onset. Male and female Wistar-Kyoto rats (WKYs) and SHRs underwent magnetic resonance imaging at weeks 7 and 9 to determine ventricular volume. Choroid plexus epiplexus cell activation, cognitive deficits, white matter atrophy, and hippocampal neuronal loss were examined at week 9. In the second phase of the experiment, male SHRs (7 weeks old) were treated with either saline or minocycline (20 mg/kg) for 14 days, and similar radiologic, histologic, and behavior tests were performed. Hydrocephalus was present at week 7 and increased at week 9 in both male and female SHRs, which was associated with greater epiplexus cell activation than WKYs. Male SHRs had greater ventricular volume and epiplexus cell activation compared to female SHRs. Minocycline administration improved cognitive function, white matter atrophy, and hippocampal neuronal cell loss. In conclusion, while both male and female SHRs developed hydrocephalus and epiplexus cell activation by week 9, it was more severe in males. Delayed minocycline treatment alleviated hydrocephalus, epiplexus macrophage activation, brain pathology, and cognitive impairment in male SHRs.

Recovery of value-added products by mining microalgae.

Microalgae blooms are always blamed for the interruption of the aquatic environment and pose a risk to the source of drinking water. Meanwhile, microalgae as primary producers are a kind of resource pool and could benefit the environment and contribute to building a circular economy. The lipid and polyhydroxybutyrate (PHB) in the cells of microalgae could be alternatives to fossil fuels and plastics, respectively, which are the culprits of global warming and plastic pollution. Besides, some microalgae are rich in nutrients, such as proteins and astaxanthin, which make themselves suitable for feed additives. As wastewater is rich in nutrients necessary for microalgae, thus, value-added product recovery via microalgae could be an approach to valorizing wastewater. However, a one-size-fits-all approach deploying various wastewater for the above products cannot be summarized. On the contrary, specific technical protocols should be tailored regarding each product in microalgae biomass with various wastewater. Thus, this review is to summarize the research effort by far on wastewater-cultivated microalgae for value-added products. Wastewater type, regulation methods, and targeted product yields are compiled and discussed and are expected to guide future extrapolation into a commercial scale.

Biologically removing vanadium(V) from groundwater by agricultural biomass.

Vanadium (V) in groundwater can pose a serious threat on both environment and health. Agricultural biomass contains solid carbon source (SCS) and could be attractive for biologically removing V(V). For this purpose, cypress sawdust, corn cob and wheat straw were selected as SCSs to remove vanadate (NaVO3). The experiments demonstrated a high efficiency of V(V) up to 98.6%, and the anaerobically biological reduction of V(V) to V(IV) by wheat straw was identified to be the best SCS by the spectrum analysis of XRD and FTIR. Along with increasing the fragment size of wheat straw, the V(V)-removal efficiency decreased, and the fragment size down to 1-3 mm was confirmed to have a significant bio-removal performance on V(V). Based on the analysis of 16s rRNA sequencing, the microbial abundance and diversity increased in the suspension liquid in the end, indicating that the microbial community could tolerate and/or detoxify V(V), besides degrading lignocellulosic materials.

Capabilities and mechanisms of microalgae on removing micropollutants from wastewater: A review.

Micropollutants in wastewater are a set of compounds receiving a growing concern to the environment and human health. As a green and low-cost process, microalgae-based systems (MBSs) have already been demonstrated the ability of micropollutant removal. In the present review, 114 micropollutants and 16 microalgae species in total are summarized and analyzed to present an overview capability of the MBSs. The analysis shows that MBSs can eradicate most of the included micropollutants with 94 compounds (82% of total) being removed by ≥ 50%. Regarding the reliability of removal efficiency, those from hormone active substances, macrolides, and cephalosporins are consistently removed at a high level (≥80%). Herein, biodegradation is the predominant removal pathway for most micropollutants, particularly, bearing electron-donating groups. Besides, the large family of microalgae species and unique phototrophic ability enables broad ecological niches and extra abilities over activated sludge systems to remove some recalcitrant micropollutants, e.g. pesticides. In the future study, optimization on the reactor configuration and operation parameters is expected to improve the stability of MBSs before extrapolating to full-scale deployment.

Rift Valley Lake as a potential magnesium source to recover phosphorus from urine.

Phosphorus recovery from urine is a sustainable approach. However, the challenge of this process is the accessibility of economically feasible magnesium sources. This study aimed to investigate the potential of low-cost Rift Valley Lake magnesium (RVL-Mg) source for phosphorus recovery from urine, where data is deficient in the source area. The effect of various operational conditions such as storage, Mg:P ratio (0.8-2.0), pH (5.5-10.5), mixing speed (30-180 rpm), urease enzyme addition (50-700 µL), urine dilution (0.11-9.0) and Ca:Mg ratio (0.3-2.5) was investigated. Under optimum operating conditions (M:P = 1.6, pH = 9, urease enzyme = 500 µL, mixing speed = 120 rpm, 60 min precipitation), the phosphorus removal efficiencies were >96% for actual and >98% for synthetic urine. During storage, spontaneous phosphorus losses were observed from synthetic (24.0%) and actual (32.0%) hydrolyzed urine due to precipitation with calcium and magnesium. The phosphorus recovery efficiency was reduced at higher (0.11:1) and lower (9:1) urine to RVL-Mg dilution, which is related to lower supersaturation of phosphorus and magnesium ions, respectively. Addition of calcium did not affect phosphorus removal efficiency, but the effect was significant on crystal product. With low (<1.0) Ca:Mg ratio, the crystal chemical analysis showed that the product has to be pure struvite (>99%), which was further identified by scanning electron microscope and X-ray diffraction to be quality struvite that might be used for agricultural purpose. Overall, low-cost magnesium ion collected from Rift Valley Lake can be a potentially candidate for sustainable phosphorus recovery from urine and any other phosphorus containing waste stream.

Comparative Study of Choke Vessel Reconstruction With Single and Multiple Perforator-Based Flaps on the Murine Back Using Delayed Surgery.

OBJECTIVE: Choke vessels, vascular anastomosis between adjacent angiosome, play an important role in flap expansion and survival. Here we established a flap model with single and multiple perforators to detect and compare the changes in choke vessels, discuss the effect of hemodynamics on the vascular morphology, and explore the underlying mechanism. METHODS: One hundred mice (7-8 weeks) were subjected to a "choke zone" surrounded by 4 perforators on their backs. Delayed surgery was performed by the ligation of 1, 2, or 3 perforators to establish flap models. The blood flow of the choke zone was measured by laser Doppler flowmetry preoperatively and 6 hours and 1, 3, 5, and 7 days. The morphological changes of choke vessels in the choke zone were observed by gross and histological analyses. Levels of angiogenesis-related markers such as endothelial nitric oxide synthase (eNOS), metalloproteinase 2, hypoxia-inducible factor 1α (HIF-1α), and intercellular adhesion molecule 2 (ICAM-2) were detected by Western blotting and enzyme-linked immunosorbent assay. RESULTS: Blood flow and microvascular count were obviously increased postoperatively and peaked and were maintained for 1 week (P < 0.01). Meanwhile, the diameters of the choke vessels expanded. The eNOS level was increased at 7 days (P < 0.05); however, the enzyme-linked immunosorbent assay results showed that the HIF-1α and ICAM-2 levels were decreased at 7 days. CONCLUSIONS: (1) The delayed surgery that kept a single perforator had the greatest impact on the choke zone. (2) Changes in choke vessels were closely related to the shear stress caused by enhanced blood perfusion after surgery. (3) Choke vessel growth was regulated by eNOS, metalloproteinase 2, HIF-1α, and ICAM-2.

Systemic immune-inflammation index is associated with cardiac complications following acute ischemic stroke: A retrospective single-center study.

BACKGROUND: Stroke-induced heart syndrome is a feared complication of ischemic stroke, that is commonly encountered and has a strong association with unfavorable prognosis. More research is needed to explore underlying mechanisms and inform clinical decision making. This study aims to explore the relationship between the early systemic immune-inflammation (SII) index and the cardiac complications after acute ischemic stroke. METHODS: Consecutive patients with acute ischemic stroke were prospectively collected from January 2020 to August 2022 and retrospectively analyzed. We included subjects who presented within 24 hours after symptom onset and were free of detectable infections or cancer on admission. SII index [(neutrophils × platelets/ lymphocytes)/1000] was calculated from laboratory data at admission. RESULTS: A total of 121 patients were included in our study, of which 24 (19.8 %) developed cardiac complications within 14 days following acute ischemic stroke. The SII level was found higher in patients with stroke-heart syndrome (p<.001), which was an independent predictor of stroke-heart syndrome (adjusted odds ratio 5.089, p=.002). CONCLUSION: New-onset cardiovascular complications diagnosed following a stroke are very common and are associated with early SII index.

Occurrence and mechanism of sulfamethoxazole in alginate-like extracellular polymers from excess sludge.

The recovery of biopolymers, particularly alginate-like extracellular polymers, from municipal sludge represents a promising step toward sustainable sludge treatment practices. Originating from wastewater plants in complexly polluted environments, alginate-like extracellular polymers carry potential environmental risks concerning their reuse. This study employs ultrahigh-performance liquid chromatography-tandem mass spectrometry to investigate the distribution coefficients and occurrence of alginate-like extracellular polymers and sulfamethoxazole. Results demonstrate a negative distribution coefficient, suggesting an inhibitory effect on sulfamethoxazole dissolution. The ethanol-extracted alginate-like extracellular polymers exhibits higher sulfamethoxazole levels (approximately 52%) than those obtained via dialysis extraction. Three-dimensional excitation-emission matrix analysis and adsorption studies indicate the absence of tyrosine-like substances in the alginate-like extracellular polymers, unlike in other extracellular polymeric substances. This absence diminishes hydrophobic interactions, highlighting that electrostatic interactions play a more important role. These insights are crucial for understanding the adsorption behavior of alginate-like extracellular polymers and optimizing their large-scale extraction processes.

Concentration properties of biopolymers via dead-end forward osmosis.

Extracellular polymeric substances (EPSs) in excess sludge of wastewater treatment plants are valuable biopolymers that can act as recovery materials. However, effectively concentrating EPSs consumes a significant amount of energy. This study employed novel energy-saving pressure-free dead-end forward osmosis (DEFO) technology to concentrate various biopolymers, including EPSs and model biopolymers [sodium alginate (SA), bovine serum albumin (BSA), and a mixture of both (denoted as BSA-SA)]. The feasibility of the DEFO technology was proven and the largest concentration ratios for these biopolymers were 94.8 % for EPSs, 97.1 % for SA, 97.8 % for BSA, and 98.4 % for BSA-SA solutions. An evaluation model was proposed, incorporating the FO membrane's water permeability coefficient and the concentrated substances' osmotic resistance, to describe biopolymers' concentration properties. Irrespective of biopolymer type, the water permeability coefficient decreased with increasing osmotic pressure, remained constant with increasing feed solution (FS) concentration, increased with increasing crossing velocity in the draw side, and showed little dependence on draw salt type. In the EPS DEFO concentration process, osmotic resistance was minimally impacted by osmotic pressure, FS concentration, and crossing velocity, and monovalent metal salts were proposed as draw solutes. The interaction between reverse diffusion metal cations and EPSs affected the structure of the concentrated substances on the FO membrane, thus changing the osmotic resistance in the DEFO process. These findings offer insights into the efficient concentration of biopolymers using DEFO.

Recovering and potentially applying of alginate like extracellular polymers from anaerobic digested sludge.

Extracellular polymeric substances (EPS) are biopolymers contained in both aerobic and anaerobic sludge. In EPS, alginate like extracellular polymers (ALE) is thought as a highly valued material, which have been widely studied with aerobic sludge. Nevertheless, a curiosity on ALE remains in anaerobic digested sludge (ADS). With 5 different sludge sources, anaerobic digestion of excess sludge was conducted in a batch mode, and then ADS was used to extract ALE and to analyze its physicochemical properties for potential applications. The yield of ALE extracted from ADS (ALE-ADS) ranged from 119.4 to 179.4 mg/g VSS. The compositional characteristics of ALE-ADS observed by FT-IR, 3D-EEM and UV-Vis spectroscopy revealed that there were minor differences in the composition and property of ALE-ADS but a similarity of 62 %-70 % to a commercial alginate remained in terms of chemical functional groups. Moreover, ALE-ADS composed of 1,4-linked ß-d-mannuronic acid (M) and 1,4 α-l-guluronic acid (G) residues that form blocks of GG (20.8 %-33.8 %), MG (12.8 %-30.1 %) and MM (6.6 %-15.1 %), respectively. Based on the gel-forming capacity, film-forming property, adsorbility, and amphiphilicity, ALE-ADS seems potential as a water-proof coating with even a better performance than the commercial alginate, as a seed coating with an increased germination rate, and as a bio-adsorbent with a similar performance to the commercial alginate and ALE from aerobic sludge.

Near-IR-Regulated Composite Hydrogel with Real-Time Infection Monitoring and a Combined Antibacterial Effect for Efficient Wound Management.

Chronic wounds induced by bacterial infection have seriously affected the health of people in the world. So, it is meaningful to develop a novel strategy with real-time infection monitoring and excellent antibacterial performance for enhancing wound management. Herein, we constructed a composite hydrogel by loading the pH indicator bromothymol blue (BTB) and gold nanocages containing 2,2'-azobis[2-(2-imidazolin-2-yl)propane] dihydrochloride (Au NCs@AIPH) into a polyacrylamide-co-poly(acrylic anhydride-modified oxidized sodium alginate) (PAM-co-PAOSA) hydrogel. In vitro and in vivo experimental results demonstrated that the composite hydrogel could effectively detect bacteria and diagnose the infection status of a wound in real time by showing visible color changes. In addition, the composite hydrogel containing Au NCs@AIPH possessed an excellent photothermal effect under near-IR (NIR) laser irradiation. The photothermal effect further activated AIPH to generate toxic free radicals to form combined antibacterial therapy for accelerating wound healing. Moreover, the composite hydrogel showed great biocompatibility. Therefore, the multifunctional hydrogel provided a novel wound management strategy for bacterial infection diagnosis and combined therapy in an infected wound.

Phosphate recovery from sludge-incinerated ash by adsorption with hydrotalcite synthesized by metals in the ash.

Selective adsorption of phosphorus (P) from the acidic leachate of sludge-incinerated ash (SIA) becomes more attractive due to avoiding removing heavy metals. Especially, layered double hydroxides (LDHs) as an anion adsorbent could be applied into this area owing to their good capacity on P-adsorption and low cost on preparation. Interestingly, SIA contains more aluminum (Al) and iron (Fe) needed to be removed prior to P-recovery, and removed Al and Fe could be utilized to synthesize LDHs, like Mg/Al-LDH and Mg/Fe-LDH. With this study, Mg/Al-LDH-r and Mg/Fe-LDH-r were economically synthesized with Al and Fe removed from SIA, which were similar in their chemical structures to commercial LDHs. The synthesized LDHs had a high P-adsorption capacity, up to 95.0%. The maximal phosphate capacity of the recovered LDHs (Mg/Al-LDH-r and Mg/Fe-LDH-r) was 239.0 and 199.8 mg P/g LDHs, respectively. "NaOH + desalinated brine" as a new desorption solution could achieve a desorption ratio at about 80%, which could reduce the liquid-solid ratio by at least 60%, greatly decreasing the desorption cost. Pot trials demonstrated that the desorbed and precipitated CaP could promote the growth of maize as well as a commercial P-fertilizer. Furthermore, the adsorbed phosphate by LDHs could be directly used as a slow-released P-fertilizer and also improve the pH value of acidic soil, completely deleting the desorption process.

[N2O Emission from the Processes of Wastewater Treatment: Mechanisms and Control Strategies].

As a direct carbon emission source, the amount of nitrous oxide (N2, which is actually caused by AOB denitrification. To control the N2O emission during biological N-removal, complete HND and NO2- accumulation for AOB denitrification should be avoided to a large extent. For this purpose, DO in aerobic tanks should be controlled at a normal level (approximately 2 mg·L-1), and solid retention time (SRT) should be extended, up to 20 d, which would avoid accumulating N2O for AOB denitrification. Additionally, external carbon should be supplemented in time to promote HDN approaching the end, N2. This review summarizes the mechanisms of all the mentioned N2O emission pathways and discusses the control strategies of N2O emission according to the associated mechanisms.

Giant tumefactive perivascular spaces.

Perivascular spaces are parts of the glymphatic pathway in the brain, which are microscopic but visible on magnetic resonance imaging when enlarged. Here, we described a case of a 16-year-old boy who presented with chronic headaches. Magnetic resonance imaging revealed giant perivascular spaces in the right centrum semiovale. Furtherly, we summarized the literature on classical and rare presentations of massive perivascular spaces and raised awareness that more clinical significance of the giant tumefactive perivascular spaces remains to be elucidated.

Performance of a continuous flow reactor on bio-reducing vanadium with straw.

Vanadium (V) in groundwater could cause a serious threat to the environment and health. Continuous flow reactors were applied to reduce V(V) with straw being a solid carbon. The reduced efficiency of V(V) in the reactor with straw and inoculated sludge reached to 71.8%-99.9% for two months' operation (after 44 d). However, a long-term operation with only straw was not satisfied, achieving the reduced efficiency of 39.2-66.6%. The SEM images clearly revealed some traces of straw surface by utilized by microbes, which implied that microbes had a stronger capacity to hydrolyze straw. The introducing external microbes were essential to achieve a better bio-reduction performance on V(V). Treponema (5.3%) with metal reduction ability and Prevotellaceae (3.3%) able to specifically degrade complex plant-derived polysaccharides were found to be dominant in the microbial community. Utilizing agricultural biomass can save a lot of normal carbon like acetate, which is of benefit for carbon emissions.

Extracting compositional blocks of alginate-like extracellular polymers (ALE) from conventional activated sludge (CAS).

As a highly added value material, alginate-like extracellular polymers (ALE) can be extracted from extracellular polymeric substances (EPS) from aerobic granular sludge (AGS). In fact, conventional activated sludge (CAS) also contains a certain amount of ALE. As CAS is widely used everywhere, waste activated sludge (WAS) from CAS is huge in its absolute amount. Although the ALE property of CAS was identified not so good as that from AGS, the mechanisms remains unclear. For this reason, it is necessary to unravel the chemically compositional blocks of ALE. Referring to natural alginate, ALE can be separated into three compositional blocks: GGL, GML and MML (like units containing guluronate or mannuronate), associated with other compositions including protein (PN), polysaccharide (PS), phosphorus (P), humic acid (HA). With real WAS from CAS, ALE was extracted and three blocks were separated: GGL = 54 %, GML = 42 % and MML = 4 % in weight, which is similar to the previous study. Moreover, the GGL blocks in CAS were obviously lower than AGS, down to by 1/3-1/2. And the GML and MML blocks in CAS were much higher than AGS, by more than 1/2. Different compositional blocks of ALE in AGS and CAS should be the reason forming different properties in applications. For this reason, a further study will be initiated to dispense/reorganize three blocks of ALE from CAS for expanding its potential applications, based on the compositional blocks of ALE from AGS.

Low-temperature drying of waste activated sludge enhanced by agricultural biomass towards self-supporting incineration.

A high moisture content of waste activated sludge (WAS) associated with a low calorific value needs to be deeply dried towards self-supporting incineration. On the other hand, thermal energy with low temperature exchanged from treated effluent has great potential for drying sludge. Unfortunately, low-temperature drying of sludge seems to be low in efficiency and long in drying time. For this reason, some agricultural biomass was added into WAS to improve the drying efficiency. The drying performance and sludge properties were analyzed and evaluated with this study. Experimental results demonstrated that wheat straw was the best in enhancing the drying performance. With only 20 % (DS/DS) of crushed wheat straw added, the average drying rate achieved up to 0.20 g water/g DS·min, much higher than 0.13 g water/g DS·min of the raw WAS. The drying time to the targeted moisture content (63 %) (for self-supporting incineration) was shortened to only 12 min, much lower than 21 min of the raw WAS. The analysis revealed that wheat straw could reduce the specific resistance of filtration (SRF) and increase the sludge filterability (X). Also, the sludge rheology, particle size distribution and SEM images could conclude that agricultural biomass played a positive role in skeleton builders, forming a mesh-like structure in sludge flocs. These special channels could obviously improve the transfer capacities of heat and water inside the sludge matrix and thus greatly increase the drying performance of WAS.

Synergy of phosphate recovery from sludge-incinerated ash and coagulant production by desalinated brine.

Wet-chemical approach is widely applied for phosphate recovery from incinerated ash of waste activated sludge (WAS), along with metals removed/recovered. The high contents of both aluminum (Al) and iron (Fe) in WAS-incinerated ash should be suitable for producing coagulants with some waste anions like Cl- and SO42- With acid (HCl) leaching and metals' removing, approximately 88 wt% of phosphorus (P) in the ash could be recovered as hydroxylapatite (HAP: Ca5(PO4)3OH); Fe3+ in the acidic leachate could be selectively removed/recovered by extraction with an organic solvent of tributyl phosphate (TBP), and thus a FeCl3-based coagulant could be synthesized by stripping the raffinate with the original brine (containing abundant Cl- and SO42-). Furthermore, a liquid poly-aluminum chloride (PAC)-based coagulant could also be synthesized with Al3+ removed from the ash and the brine, which behaved almost the same in the coagulation performance as a commercial coagulant on both phosphate and turbidity removals. Both P-recovery from the ash and coagulant production associated with the brine would enlarge the markets of both 'blue' phosphate and 'green' coagulants.

Calcium Alginate Production through Forward Osmosis with Reverse Solute Diffusion and Mechanism Analysis.

Calcium alginate (Ca-Alg) is a novel target product for recovering alginate from aerobic granular sludge. A novel Ca-Alg production method was proposed herein where Ca-Alg was formed in a sodium alginate (SA) feed solution (FS) and concentrated via forward osmosis (FO) with Ca2+ reverse osmosis using a draw solution of CaCl2. An abnormal reverse solute diffusion was observed, with the average reverse solute flux (RSF) decreasing with increasing CaCl2 concentrations, while the average RSF increased with increasing alginate concentrations. The RSF of Ca2+ in FS decreased continuously as the FO progressed, using 1.0 g/L SA as the FS, while it increased initially and later decreased using 2.0 and 3.0 g/L SA as the FS. These results were attributed to the Ca-Alg recovery production (CARP) formed on the FO membrane surface on the feed side, and the percentage of Ca2+ in CARP to total Ca2+ reverse osmosis reached 36.28%. Scanning electron microscopy and energy dispersive spectroscopy also verified CARP existence and its Ca2+ content. The thin film composite FO membrane with a supporting polysulfone electrospinning nanofiber membrane layer showed high water flux and RSF of Ca2+, which was proposed as a novel FO membrane for Ca-Alg production via the FO process with Ca2+ reverse diffusion. Four mechanisms including molecular sieve role, electrification of colloids, osmotic pressure of ions in CARP, and FO membrane structure were proposed to control the Ca-Alg production. Thus, the results provide further insights into Ca-Alg production via FO along with Ca2+ reverse osmosis.