Metatranscriptomics reveals diversity of symbiotic interaction and mechanisms of carbon exchange in the marine cyanolichen Lichina pygmaea.

Lichens are exemplar symbioses based upon carbon exchange between photobionts and their mycobiont hosts. Historically considered a two-way relationship, some lichen symbioses have been shown to contain multiple photobiont partners; however, the way in which these photobiont communities react to environmental change is poorly understood. Lichina pygmaea is a marine cyanolichen that inhabits rocky seashores where it is submerged in seawater during every tidal cycle. Recent work has indicated that L. pygmaea has a complex photobiont community including the cyanobionts Rivularia and Pleurocapsa. We performed rRNA-based metabarcoding and mRNA metatranscriptomics of the L. pygmaea holobiont at high and low tide to investigate community response to immersion in seawater. Carbon exchange in L. pygmaea is a dynamic process, influenced by both tidal cycle and the biology of the individual symbiotic components. The mycobiont and two cyanobiont partners exhibit distinct transcriptional responses to seawater hydration. Sugar-based compatible solutes produced by Rivularia and Pleurocapsa in response to seawater are a potential source of carbon to the mycobiont. We propose that extracellular processing of photobiont-derived polysaccharides is a fundamental step in carbon acquisition by L. pygmaea and is analogous to uptake of plant-derived carbon in ectomycorrhizal symbioses.

The genome sequence of the John Dory, Zeus faber Linnaeus, 1758.

We present a genome assembly from an individual Zeus faber (the John Dory; Chordata; Actinopteri; Zeiformes; Zeidae). The genome sequence is 804.7 megabases in span. Most of the assembly is scaffolded into 22 chromosomal pseudomolecules. The mitochondrial genome has also been assembled and is 16.72 kilobases in length.

The genome sequence of the thickback sole, Microchirus variegatus (Donovan, 1808).

We present a genome assembly from an individual female Microchirus variegatus (the thickback sole; Chordata; Actinopteri; Pleuronectiformes; Soleidae). The genome sequence is 724.7 megabases in span. Most of the assembly is scaffolded into 23 chromosomal pseudomolecules. The mitochondrial genome has also been assembled and is 17.42 kilobases in length.

The genome sequence of a scale worm, Harmothoe impar (Johnston, 1839).

We present a genome assembly from an individual scale worm, Harmothoe impar; Annelida; Polychaeta; Phyllodocida; Polynoidae). The genome sequence is 1,512.3 megabases in span. Most of the assembly is scaffolded into 18 chromosomal pseudomolecules. The mitochondrial genome has also been assembled and is 15.37 kilobases in length.

The genome sequence of the light-bulb sea squirt, Clavelina lepadiformis (Müller, 1776).

We present a genome assembly from an individual Clavelina lepadiformis (the light-bulb sea squirt; Chordata; Ascidiacea; Aplousobranchia; Clavelinidae). The genome sequence is 210.1 megabases in span. Most of the assembly is scaffolded into 9 chromosomal pseudomolecules. The mitochondrial genome has also been assembled and is 14.48 kilobases in length.

The genome sequence of the European plaice, Pleuronectes platessa (Linnaeus, 1758).

We present a genome assembly from an individual Pleuronectes platessa(the European plaice; Chordata; Actinopteri; Pleuronectiformes; Pleuronectidae). The genome sequence is 687.4 megabases in span. Most of the assembly is scaffolded into 24 chromosomal pseudomolecules. The mitochondrial genome has also been assembled and is 17.4 kilobases in length.

The genome sequence of the turban top shell, Gibbula magus (Linnaeus, 1758).

We present a genome assembly from an individual Gibbula magus (the turban top shell; Mollusca; Gastropoda; Trochida; Trochidae). The genome sequence is 1,470 megabases in span. Most of the assembly is scaffolded into 18 chromosomal pseudomolecules. The mitochondrial genome has also been assembled and is 16.1 kilobases in length. Gene annotation of this assembly on Ensembl identified 41,167 protein coding genes.

The genome sequence of the orange-striped anemone, Diadumene lineata (Verrill, 1869).

We present a genome assembly from an individual Diadumene lineata (the orange-striped anemone; Cnidaria; Anthozoa; Actiniaria; Diadumenidae). The genome sequence is 313 megabases in span. The majority of the assembly (96.03%) is scaffolded into 16 chromosomal pseudomolecules. The complete mitochondrial genome was also assembled and is 17.6 kilobases in length.

The genome sequence of Aplidium turbinatum (Savigny 1816), a colonial sea squirt.

We present a genome assembly from an individual Aplidium turbinatum (Chordata; Ascidiacea; Aplousobranchia; Polyclinidae). The genome sequence is 605 megabases in span. The majority of the assembly (99.98%) is scaffolded into 18 chromosomal pseudomolecules. The complete mitochondrial genome was also assembled and is 18.4 kilobases in length.

The genome sequence of the grey top shell, Steromphala cineraria (Linnaeus, 1758).

We present a genome assembly from an individual Steromphala cineraria (the grey topshell; Mollusca; Gastropoda; Trochida; Trochidae). The genome sequence is 1,270 megabases in span. Most of the assembly (99.23%) is scaffolded into 18 chromosomal pseudomolecules.