Assessment of operant learning and memory in mice born through ICSI.

STUDY QUESTION: Are there differences in operant learning and memory between mice born through ICSI and naturally conceived control (CTL) mice? SUMMARY ANSWER: ICSI females exhibited deficits in the acquisition reward learning relative to CTL females, and ICSI males exhibited deficiencies in discrimination learning and memory relative to CTL males. WHAT IS KNOWN ALREADY: Some human outcome studies have suggested that ICSI might be associated with an increased risk of certain cognitive disorders, but only one of two behavioral studies with ICSI mouse models have reported differences between ICSI and CTL females. No studies to date have investigated associative learning in ICSI mice. STUDY DESIGN, SIZE, DURATION: Groups of 36 ICSI mice (18 male, 18 female) and 37 CTL mice (19 male, 18 female) aged 3-6 months were compared in a series of operant learning procedures that assessed acquisition of a new behavior, discrimination learning and memory. In total, 16 ICSI mice (9 male, 7 female) and 17 CTL mice (10 male, 7 female) received follow-up discrimination learning and memory assessments at 12 months of age (6 months after the end of initial training) to evaluate retention and reacquisition of learned performances. PARTICIPANTS/MATERIALS, SETTING, METHODS: Mice received daily operant learning sessions in experimental chambers in which all stimulus events and the recording of responses were automated. Food rewards were delivered for responding under different conditions of reinforcement, which varied by procedure. Subjects received a successive series of sessions of nose poke acquisition training, discrimination training and the delayed-non-matching-to-position memory procedure. Mixed repeated measures ANOVAs in which the between-subjects factor was group (ICSI vs CTL) and the within-subjects factor was repeated exposures to learning procedures (i.e. sessions) were used to analyze data. MAIN RESULTS AND THE ROLE OF CHANCE: In comparisons between all mice (i.e. males and females combined), CTL mice exhibited superior performance relative to ICSI in response acquisition (P = 0.03), discrimination (P = 0.001) and memory (P = 0.007). Sex-specific comparisons between the groups yielded evidence of sexual dimorphism. ICSI females exhibited a deficit in acquisition learning relative to CTL females (P < 0.001), but there was not a significant difference between CTL and ICSI males. In the discrimination and memory tasks, ICSI males exhibited deficits relative to CTL males (P = 0.002 and P = 0.02, respectively) but the differences between females in these tasks were not significant. There was no difference in discrimination or memory retention/re-acquisition assessments conducted with mice at 12 months of age. ICSI males and females weighed significantly more than CTL counterparts at all points during the experiment. LARGE SCALE DATA: N/A. LIMITATIONS, REASONS FOR CAUTION: The study was not blinded. All learning assessments utilized food reward; other assessments of operant, Pavlovian and nonassociative learning are needed to fully characterize learning in ICSI mice and speculate regarding the implications for cognitive function in humans conceived via ICSI. WIDER IMPLICATIONS OF THE FINDINGS: Studying learning and memory processes in mouse models have the potential to shed light on ICSI outcomes at the level of cognitive function. Future research should use multiple learning paradigms, assess both males and females, and investigate the effects of variables related to the ICSI procedure. Studying cognitive function in ICSI is an interdisciplinary endeavor and requires co-ordination between researchers at the genetic and psychological levels of analysis. STUDY FUNDING/COMPETING INTEREST(S): This work was supported, in part, by grants from the NIH (P30GM110767, HD071736 and HD085506 to W.Y.), the Templeton Foundation (61174 to W.Y.) and a New Scholarly Endeavor Grant from the University of Nevada, Reno Office of Research and Innovation (to M.L., Y.W., H.Z., L.H. and W.Y.). The authors declare no competing interests.

Hardening or softening? An observational study of changes to the prevalence of hardening indicators in Victoria, Australia, 2001-2016.

BACKGROUND: The hardening hypothesis predicts that as smoking prevalence declines, remaining smokers will be more heavily addicted to nicotine and/or less interested in quitting. We tested this hypothesis in a population exposed to a comprehensive tobacco control programme over a 16-year period. METHODS: Annual cross-sectional surveys randomly sampled adults (aged 26+) in the state of Victoria, Australia, between 2001 and 2016. Until 2010, participants were recruited through random digit dialling to landline telephones; from 2011, sampling frames also included mobile phones. Logistic regressions assessed changes over time in the prevalence of smoking and each hardening indicator; additional models examined interactions by sex, age, education and socioeconomic status. RESULTS: Smoking prevalence declined significantly between 2001 and 2016 (20.1%-13.0%), as did the prevalence of seven hardening indicators: daily smoking, heavy consumption, no quit attempt in the past 5 years or past 12 months, no intention to quit in the next 6 months or next 30 days, and happiness to keep smoking. In addition, the proportion of smokers defined as 'hardcore' decreased from 17.2% to 9.1%. On the whole, hardening indicators decreased to a similar extent among demographic subgroups. CONCLUSIONS: These results are inconsistent with the hardening hypothesis. Rather, they suggest that a comprehensive tobacco control programme that combines provision of cessation support to individual smokers with implementation of population-level interventions to drive all smokers towards quitting, can successfully reduce both smoking prevalence and levels of dependence and desire to keep smoking among the remaining population of smokers.

The Origin and Evolution of the Behavior Analysis Program at the University of Nevada, Reno.

The origins of the Behavior Analysis program at the University of Nevada, Reno by way of a self-capitalized model through its transition to a more typical graduate program is described. Details of the original proposal to establish the program and the funding model are described. Some of the unusual features of the program executed in this way are discussed, along with problems engendered by the model. Also included is the diversification of faculty interests over time. The status of the program, now, after 25 years of operation, is presented.

A Historical Perspective on the Future of Behavior Science.

Like all natural sciences, behavior science has much to offer toward an understanding of the world. The extent to which the promise of behavior science is realized, though, depends upon the extent to which we keep what we know before us. This paper considers fundamental concepts in behavior science, including the concepts of behavior, stimulation, setting conditions, and language. In considering these concepts, we revisit comments from B. F. Skinner and J. R. Kantor and also consider some areas of behavior analytic research and the implications they have for reconsidering long-held assumptions about the analysis of behavior. We hope that, in considering our foundations, the vitality and strength of the discipline might be enhanced, our impact on science improved, and our future secured.

Surveying alcohol and other drug use through telephone sampling: a comparison of landline and mobile phone samples.

BACKGROUND: Telephone surveys based on samples of landline telephone numbers are widely used to measure the prevalence of health risk behaviours such as smoking, drug use and alcohol consumption. An increasing number of households are relying solely on mobile telephones, creating a potential bias for population estimates derived from landline-based sampling frames which do not incorporate mobile phone numbers. Studies in the US have identified significant differences between landline and mobile telephone users in smoking and alcohol consumption, but there has been little work in other settings or focussed on illicit drugs. METHODS: This study examined Australian prevalence estimates of cannabis use, tobacco smoking and risky alcohol consumption based on samples selected using a dual-frame (mobile and landline) approach. Respondents from the landline sample were compared both to the overall mobile sample (including respondents who had access to a landline) and specifically to respondents who lived in mobile-only households. Bivariate comparisons were complemented with multivariate logistic regression models, controlling for the effects of basic demographic variables. RESULTS: The landline sample reported much lower prevalence of tobacco use, cannabis use and alcohol consumption than the mobile samples. Once demographic variables were adjusted for, there were no significant differences between the landline and mobile respondents on any of the alcohol measures examined. In contrast, the mobile samples had significantly higher rates of cannabis and tobacco use, even after adjustment. Weighted estimates from the dual-frame sample were generally higher than the landline sample across all substances, but only significantly higher for tobacco use. CONCLUSIONS: Landline telephone surveys in Australia are likely to substantially underestimate the prevalence of tobacco smoking by excluding potential respondents who live in mobile-only households. In contrast, estimates of alcohol consumption and cannabis use from landline surveys are likely to be broadly accurate, once basic demographic weighting is undertaken.

A store cohort study of compliance with a point-of-sale cigarette display ban in Melbourne, Australia.

INTRODUCTION: This study aimed to evaluate compliance with legislation which restricted cigarette displays in retail outlets, and to assess prevalence of pro- and anti-tobacco elements in stores pre- and post-legislation. METHODS Three audits of 302 stores in Melbourne, Australia by trained observers who gathered information on point-of-sale tobacco displays 2-3 months before and 3-4 and 11-12 months after the enactment of new restrictions. RESULTS: Between the first and second audits, nine stores stopped selling tobacco and three stores had either shut down or were closed for renovations. Of the remaining 290 stores, 94.1% observed the full ban on cigarette package visibility, while new restrictions on price board size and new requirements for graphic health warnings were followed in 85.9% and 67.2% of stores, respectively. Between the second and third audits, another seven stores ended tobacco sales and two stores closed. In Audit 3, 89.7% of the remaining 281 stores complied with price board restrictions, and 82.2% of stores followed requirements for graphic health warnings. Overall, the prevalence of anti-tobacco signage increased and pro-tobacco features decreased between audits for every store type and neighborhood socio-economic status. CONCLUSIONS: Tobacco retailers were almost universally compliant with placing cigarettes out of sight and a substantial majority were compliant with regulations on price board size and display of graphic health warnings, demonstrating that such legislation can be implemented successfully.

What price quitting? The price of cigarettes at which smokers say they would seriously consider trying to quit.

BACKGROUND: Deciding on an appropriate level for taxes on tobacco products is a critical issue in tobacco control. The aim of the present study was to describe the critical price points for packs for smokers of each pack size, to calculate what this would equate to in terms of price per stick, and to ascertain whether price points varied by age, socio-economic status and heaviness of smoking. METHODS: In November 2011, 586 Victorian smokers of factory-made cigarettes were asked during a telephone survey about their usual brand, including the size and cost of their usual pack. They were also asked about use of illicit tobacco. Smokers estimated what price their preferred pack would need to reach before they would seriously consider quitting. RESULTS: Three-quarters of regular smokers of manufactured cigarettes could envisage their usual brand reaching a price at which they would seriously consider quitting. Analyses revealed that answers clustered around whole numbers, (AUD$15, $20, $25 and $30), with a median nominated price point of AUD$20 per pack. The median price point at which regular smokers would consider quitting was calculated to be 80 cents per stick, compared to the current median reported stick price of 60 cents.Of the smokers who nominated a price point, 60.1% indicated they would seriously consider quitting if the cost of their usual brand equated to 80 cents per stick or less; 87.5% would seriously consider quitting if sticks reached one dollar each. CONCLUSIONS: These results do suggest a potentially useful approach to setting taxes in Australia. If taxes can be set high enough to ensure that the cost of the smokers' preferred packs exceeds critical price points, then it seems likely that more people would seriously attempt to quit than if the price increased to a level even slightly below the price points. Our study suggests that a tax increase large enough to ensure that a typical pack of 25 cigarettes in Australia cost at least AUD$20 would prompt more than 60% of smokers able to nominate a price point to seriously think about quitting, with particularly strong effects among low-SES smokers.

Factors influencing child mental health: A state-wide survey of Victorian children.

AIMS: This study aims to estimate the prevalence of mental health problems among Victorian children and to investigate factors associated with poorer mental health. METHOD: Computer-assisted telephone interviews were undertaken with the parents of 3370 randomly selected Victorian children aged 4 to 12 years. They reported on their child's mental health and special health-care needs as well as their own mental health, family functioning and a range of community and socio-demographic variables. Population estimates and odds ratios (OR) were calculated with 95% confidence intervals (95% CI). RESULTS: Overall, 11.6% (95% CI = 10.3-12.9%) of Victorian children were estimated to be at risk of having mental health problems. Factors independently placing children at increased risk of mental health problems that were 'of concern' include a child having special health-care needs (OR = 7.89, 95% CI 5.16 to 12.08), unhealthy family functioning (OR = 3.84, 95% CI 2.19 to 6.74), parental mental health problems (OR = 7.89, 95% CI 5.16 to 12.08), neighbourhood safety (OR = 2.47, 95% CI 1.20 to 5.07) and area of residence (OR = 2.01, 95% CI 1.33 to 3.02). CONCLUSIONS: A significant proportion of Victorian children are at some risk of mental health problems. These limited but important predictors of children's mental health reinforce the need for policy solutions that will extend beyond those offered by traditional mental health service systems.

Constructs, Events, and Acceptance and Commitment Training.

The present article considers acceptance and commitment training (ACT) from the perspective of interbehavioral psychology. Specifically, J. R. Kantor's (1957) explicit distinction between constructs and events is reviewed, with particular attention given to the use of ACT in the practice settings of applied behavior analysis. It is recommended that practitioners be especially sensitive to the distinction between constructs and events as they consider employing ACT interventions. The interbehavioral field construct of interbehavioral psychology is briefly described as a context for conceptualizing both practice and research related to ACT in behavior analysis. Related conceptual issues, especially issues pertaining to the subject matter of behavior analysis and the Skinnerian concept of private events, are considered. The potential value of further integrating interbehavioral thinking into ACT practice and research is described.

Direct Instruction of Absolute Pitch Using the Theremin as a Musical Instrument and Experimental Apparatus.

Direct Instruction (DI) is a method of education that has historically been applied to improve academic behaviors. Though DI has a modest history of teaching musical literacy skills, its application in teaching music performance skills has been limited. This article presents two methods derived from DI principles to teach the advanced musical skill of absolute pitch using the theremin as a unique musical instrument and experimental apparatus. The two methods are optimized for either fast learning of the new skill or assessment of the skill in a general sample of participants, and both are shown to significantly improve posttraining performance. Instructors recruited 53 college aged participants with a variety of music education histories across two studies (16 participants in Study 1; 37 participants in Study 2) for participation in either of the novel DI protocols for teaching absolute pitch using prompt fading. All participants showed significantly improved absolute pitch accuracy above baseline following 1 hr or less of DI with either method. Implications and suggestions for educators and researchers are discussed.

Motivating operations as contexts for operant discrimination training and testing.

Two groups of mice were exposed to stimulus discrimination training and testing under different motivational conditions to study interactions between motivating operations (MOs) during initial discrimination training and MOs when performance is tested following training. One group received all discrimination training sessions under 24-h food deprivation while the other received all sessions under 0-h food deprivation. The number of responses allowed during discrimination training sessions was limited such that the two groups experienced the same number of response-outcome contingencies. The groups then received two post-discrimination training tests: one conducted under 24-h food deprivation and the other conducted under 0-h food deprivation. Results indicated no difference between groups in terms of discrimination ratio. However, subjects trained under 24-h deprivation made more responses in the 24-h test, while subjects trained under 0-h deprivation made more responses in the 0-h test. These results are discussed in terms of motivational state-dependent learning.

Relations between Description and Experimentation in the Metacontingency Enterprise: An Interbehavioral Analysis.

Despite extensive theoretical development, there is a lack of consensus in the metacontingency enterprise on the extent to which current metacontingency constructs describe experimental happenings. The purpose of this article is to provide an interbehavioral analysis of the metacontingency enterprise that examines relations between description and experimentation in order to facilitate research on cultural selection occurring through metacontingencies. In particular, this article considers how stimulus functions of descriptions of metacontingency constructs participate in metacontingency experiments in terms of specificity, types of analysis, levels of analysis, and procedures. The extent to which experimental findings are able to be described in terms of metacontingency constructs is assessed. Prominent events and relations demonstrated by metacontingency experiments are summarized and discussed, as well as inconsistencies between relations described and relations constructed based on events observed. Recommendations for experimental and descriptive adjustments are offered. Although this analysis may or may not have any bearing on the metacontingency enterprise, it may serve as a template for conducting interbehavioral analyses of activities in other enterprises, if not more analyses of the metacontingency enterprise.

Cutaneous Lesions in Freshwater Turtles (Emydura macquarii krefftii and Myuchelys latisternum) in a Rainforest Creek in North Queensland, Australia.

Freshwater turtles inhabit most rivers and creeks on the east coast of Australia, but some species are only found in specific catchments, which makes them vulnerable to extinction. During annual fieldtrips to Alligator Creek, North Queensland, the resident population of Myuchelys latisternum and Emydura macquarii krefftii in a natural pond, just outside Bowling Green National Park, have been surveyed for a number of years and demographic data recorded against tagged turtles. Rounded, cutaneous lesions on individual animals were first noted in August 2016, three years after the first survey of the population. Turtles living in the upstream sections of the creek were not affected. An initial investigation into the cause of the lesions ruled out pollutants and although the bacterial communities appeared to be different on turtles with lesions, a causative agent was not identified. Attempts to isolate virus in culture was not successful and specific PCRs for ranavirus, papillomavirus, adenovirus and herpesvirus did not identify their presence. Blood biochemical parameters, body condition and activity levels were not significantly different between affected turtles and those without lesions. The turtles in this pond were monitored regularly over the following three years with 249 M. latisternum and 192 E. m. krefftii captured, tagged and released. The prevalence of the lesions fluctuated with season from 0 to 77 and 68% respectively, but did not vary significantly between species or sex in adults. There was a tendency for larger animals to be more likely to have lesions. The position of the lesions on the turtles was mostly on dorsal surfaces, distally on the legs and proximal on the tales of males, indicating that the initial lesion may have been associated with a behaviourally induced trauma. Recaptured animals (n = 43) during this period, provided records of lesion progression over time and while some healed up between capture events, others persisted for up to 24 months. Some turtles were repeatedly captured without lesions. Intra-species aggression associated with seasonal behaviours could potentially be the primary cause of skin trauma, followed by a secondary invasion of an unusual pathogen present in the environment.

Interbehavioral psychology and the behavior systems framework: A brief reply.

The present commentary considers a paper by Silva, Silva, and Machado (2019) published in this special issue, which describes some relations between Behavior Systems Theory and Interbehavioral Psychology. In particular, the systems aspects, field orientation, and role of experimentation in both Behavior Systems Theory and Interbehavioral Psychology are discussed. Similarities and differences among the two perspectives are highlighted, and misconceptions about Interbehavioral Psychology are addressed.

The Will to Fight: Aversion-Induced Aggression and the Role of Motivation in Intergroup Conflicts.

Aggressive behavior is a source of many significant human problems, most notably the catastrophic loss of life and resources that can result from violent conflicts between groups. Aggressive behavior is particularly likely to arise from aversive conditions that function as motivating operations (MOs) that establish the stimulation produced by aggressive acts as reinforcing. We describe the behavior that arises from these circumstances as aversion-induced aggression (AIA) and argue that the MOs associated with AIA are important factors in initiating and sustaining violent conflicts between groups. In support of this, we survey the basic nonhuman research that has demonstrated the aggression-motivating functions of aversive stimuli. We extend our analysis of AIA to humans and describe how the special properties of verbal stimuli serve as the basis for notable differences between AIA in humans and nonhumans. We describe how aversive conditions may be exploited by leaders to establish support for aggression against another group in the pursuit of their objectives. We suggest that conflicts between groups cannot be resolved in the long term unless the motivational conditions from which conflicts arise are alleviated. Aggression is rarely effective in this regard because it exacerbates these conditions. For this reason, we advocate against the use of aggression as a tool for resolving conflicts between groups and consider how behavior science may contribute to the development and evaluation of alternative nonviolent practices.

Interactions between the effects of food and water motivating operations on food- and water-reinforced responding in mice.

Two experiments examined interactions between the effects of food and water motivating operations (MOs) on the food- and water-reinforced operant behavior of mice. In Experiment 1, mice responded for sucrose pellets and then water reinforcement under four different MOs: food deprivation, water deprivation, concurrent food and water deprivation, and no deprivation. The most responding for pellets occurred under food deprivation and the most responding for water occurred under water deprivation. Concurrent food and water deprivation decreased responding for both reinforcers. Nevertheless, water deprivation alone increased pellet-reinforced responding and food deprivation alone likewise increased water-reinforced responding relative to no deprivation. Experiment 2 demonstrated that presession food during concurrent food and water deprivation increased in-session responding for water relative to sessions where no presession food was provided. Conversely, presession water during concurrent food and water deprivation did not increase in-session responding for pellets. These results suggest that a) the reinforcing value of a single stimulus can be affected by multiple MOs, b) a single MO can affect the reinforcing value of multiple stimuli, and c) reinforcing events can also function as MOs. We consider implications for theory and practice and suggest strategies for further basic research on MOs.

Predicting the Effects of Interventions: A Tutorial on the Disequilibrium Model.

The disequilibrium approach to reinforcement and punishment, derived from the probability-differential hypothesis and response deprivation hypothesis, provides a number of potentially useful mathematical models for practitioners. The disequilibrium approach and its accompanying models have proven effective in the prediction and control of behavior, yet they have not been fully espoused and integrated into clinical practice. The purpose of this tutorial is to detail the disequilibrium approach and adapt its mathematical models for use as a tool in applied settings. The disequilibrium models specify how to arrange contingencies and predict the effects of those contingencies. We aggregate these models, and provide them as a single tool, in the form of a Microsoft Excel® spreadsheet that calculates the direction and magnitude of behavior change based on baseline measures and a practitioner's choice of intervention parameters. How practitioners take baseline measures and select intervention parameters in accordance with disequilibrium models is explicated. The proposed tool can be accessed and downloaded for use at https://osf.io/knf7x/.