Corrected and Republished from: "Isolation and Characterization of the First Freshwater Cyanophage Infecting Pseudanabaena".

Cyanobacteria are the major primary producers in both freshwater and marine environments. However, the majority of freshwater cyanophages remain unknown due to the limited number of cyanophage isolates. In this study, we present a novel lytic freshwater cyanophage, PA-SR01, which was isolated from the Singapore Serangoon Reservoir. To our knowledge, this is the first isolate of a cyanophage that has been found to infect the cyanobacterium Pseudanabaena. PA-SR01 has a narrow host range, a short latent period, and is chloroform sensitive. PA-SR01 is a member of Siphoviridae with a long noncontractile tail. It is a double-stranded DNA virus with a 137,012-bp genome. Functional annotation for the predicted open reading frames (ORFs) of the PA-SR01 genome identified genes with putative functions related to DNA metabolism, structural proteins, lysis, host-derived metabolic genes, and DNA packaging. Out of 166 predicted ORFs, only 17 ORFs have homology with genes with known function. Phylogenetic analysis of the major capsid protein and terminase large subunit further suggests that phage PA-SR01 is evolutionary distinct from known cyanophages. Metagenomics sequence recruitment onto the PA-SR01 genome indicates that PA-SR01 represents a new evolutionary lineage of phage which shares considerable genetic similarities with phage sequences in aquatic environments and could play key ecological roles. IMPORTANCE This study presents the isolation of the very first freshwater cyanophage, PA-SR01, that infects Pseudanabaena, and fills an important knowledge gap on freshwater cyanophages as well as cyanophages infecting Pseudanabaena.

Predicting Antibiotic Resistance and Assessing the Risk Burden from Antibiotics: A Holistic Modeling Framework in a Tropical Reservoir.

Predicting the hotspots of antimicrobial resistance (AMR) in aquatics is crucial for managing associated risks. We developed an integrated modeling framework toward predicting the spatiotemporal abundance of antibiotics, indicator bacteria, and their corresponding antibiotic-resistant bacteria (ARB), as well as assessing the potential AMR risks to the aquatic ecosystem in a tropical reservoir. Our focus was on two antibiotics, sulfamethoxazole (SMX) and trimethoprim (TMP), and on Escherichia coli (E. coli) and its variant resistant to sulfamethoxazole-trimethoprim (EC_SXT). We validated the predictive model using withheld data, with all Nash-Sutcliffe efficiency (NSE) values above 0.79, absolute relative difference (ARD) less than 25%, and coefficient of determination (R2) greater than 0.800 for the modeled targets. Predictions indicated concentrations of 1-15 ng/L for SMX, 0.5-5 ng/L for TMP, and 0 to 5 (log10 MPN/100 mL) for E. coli and -1.1 to 3.5 (log10 CFU/100 mL) for EC_SXT. Risk assessment suggested that the predicted TMP could pose a higher risk of AMR development than SMX, but SMX could possess a higher ecological risk. The study lays down a hybrid modeling framework for integrating a statistic model with a process-based model to predict AMR in a holistic manner, thus facilitating the development of a better risk management framework.

Enhanced thermophilic dark fermentation of hydrogen production from food waste by Fe-modified biochar.

The industrialization of hydrogen production through dark fermentation of food waste faces challenges, such as low yields and unpredictable fermentation processes. Biochar has emerged as a promising green additive to enhance hydrogen production in dark fermentation. Our study demonstrated that the introduction of Fe-modified biochar (Fe-L600) significantly boosted hydrogen production during thermophilic dark fermentation of food waste. The addition of Fe-L600 led to a remarkable 31.19% increase in hydrogen yield and shortened the time needed for achieving stabilization of hydrogen production from 18 h to 12 h. The metabolite analysis revealed an enhancement in the butyric acid pathway as the molar ratio of acetic acid to butyric acid decreased from 3.09 to 2.69 but hydrogen yield increased from 57.12 ± 1.48 to 76.78 ± 2.77 mL/g, indicating Fe-L600 improved hydrogen yield by regulating crucial metabolic pathways of hydrogen production. The addition of Fe-L600 also promoted the release of Fe2+ and Fe3+ and increased the concentrations of Fe2+ and Fe3+ in the fermentation system, which might promote the activity of hydrogenase and ferredoxin. Microbial community analysis indicated a substantial increase in the relative abundance of Thermoanaerobacterium after thermophilic dark fermentation. The relative abundances of microorganisms responsible for hydrolysis and acidogenesis were also observed to be improved in the system with Fe-L600 addition. This research provides a feasible strategy for improving hydrogen production of food waste and deepens the understanding of the mechanisms of biochar.

Picocyanobacterial-bacterial interactions sustain cyanobacterial blooms in nutrient-limited aquatic environments.

Cyanobacterial blooms (CBs) and concomitant water quality issues in oligotrophic/mesotrophic waters have been recently reported, challenging the conventional understanding that CBs are primarily caused by eutrophication. To elucidate the underlying mechanism of CBs in nutrition-deficient waters, the changes in Chlorophyll a (Chl-a), cyanobacterial-bacterial community composition, and certain microbial function in Qingcaosha Reservoir, the global largest tidal estuary storage reservoir, were analyzed systematically and comprehensively after its pilot run (2011-2019) in this study. Although the water quality was improved and stabilized, more frequent occurrences of bloom level of Chl-a (>20 µg L-1) in warm seasons were observed during recent years. The meteorological changes (CO2, sunshine duration, radiation, precipitation, evaporation, and relative humidity), water quality variations (pH, total organic carbon content, dissolved oxygen, and turbidity), accumulated sediments as an endogenous source, as well as unique estuarine conditions collectively facilitated picocyanobacterial-bacterial coexistence and community functional changes in this reservoir. A stable and tight co-occurrence pattern was established between dominant cyanobacteria (Synechococcus, Cyanobium, Planktothrix, Chroococcidiopsis, and Prochlorothrix) and certain heterotrophic bacteria (Proteobacteria, Actinobacteria, and Bacteroidetes), which contributed to the remineralization of organic matter for cyanobacteria utilization. The relative abundance of chemoorganoheterotrophs and bacteria related to nitrogen transformation (Paracoccus, Rhodoplanes, Nitrosomonas, and Zoogloea) increased, promoting the emergence of CBs in nutrient-limited conditions through enhanced nutrient recycling. In environments with limited nutrients, the interaction between photosynthetic autotrophic microorganisms and heterotrophic bacteria appears to be non-competitive. Instead, they adopt complementary roles within their ecological niche over long-term succession, mutually benefiting from this association. This long-term study confirmed that enhanced nutrient cycling, facilitated by cyanobacterial-bacterial symbiosis following long-term succession, could promote CBs in oligotrophic aquatic environments devoid of external nutrient inputs. This study advances understanding of the mechanisms that trigger and sustain CBs under nutritional constraints, contributing to developing more effective mitigation strategies, ensuring water safety, and maintaining ecological balance.

External carbon source as a viable tool for controlling antibiotics and antibiotic resistance genes (ARGs) in effluent: Influence on antibiotic removal and ARGs dissemination.

Fluoroquinolone antibiotics and antibiotic resistance genes (ARGs) regarded as emerging contaminants were poorly removed in conventional wastewater treatment plants (WWTPs). Nitrogen-containing heterocyclic organics were found to be biodegraded through denitrification co-metabolism. The feasibility to enhance antibiotics removal efficiency in WWTPs through denitrification co-metabolism needs to be further verified. Meanwhile, due to significant correlation between ARGs profiles and nitrogen removal that was previously observed, the dissemination of ARGs during denitrification was worthy of in-depth understanding. Herein, the antibiotic removal and ARGs dissemination in denitrification co-metabolism condition were investigated with different denitrifying consortiums that acclimated under different conditions in terms of carbon source and the exposure of Ofloxacin (OFL). The results suggest that the removal of OFL can be enhanced by the denitrification co-metabolism. The tolerance to OFL is different among various denitrifying communities. For the denitrifying consortiums acclimated with methanol, long-term exposure to trace OFL (1 µg/L) could reduce the capabilities of removal and tolerance to OFL. On the contrary, those acclimated with sodium acetate (NaAc), the capabilities of removal and tolerance to OFL, were enhanced by long-term exposure to trace OFL. According to the quantitative determination to 384 target genes with high-throughput quantitative PCR, the abundance of ARGs in consortiums greatly increased when exposed to OFL at the concentration of comparable to sewage, which was also much larger than that acclimated with methanol. It can be confirmed and supported by DNA sequencing results that the antibiotic removal and the dissemination of ARGs were determined by microbial community that could be shaped with carbon source. These conclusions suggest that selecting the right external carbon source can be a useful strategy for WWTPs to control antibiotics and ARGs in the effluent. From a new perspective on mitigating ARGs dissemination, NaAc was not an appropriate carbon source.

Novel Role of Hematite in Anaerobic Digestion: Manipulating Membrane-Bound Electron Transport Chain by the Construction of Biological Capacitors with Humic Acid.

Humic acid (HA) has attracted much attention for its electron-competitive effect of quinone groups on anaerobic methanogenesis. This study analyzed the biological "capacitor" to determine how it might effectively reduce electron competition. As biological capacitor-producing additives, three semiconductive materials, including magnetite, hematite, and goethite, were selected. The results showed that hematite and magnetite could significantly alleviate the inhibited methanogenesis caused by the HA model compound anthraquinone-2,6-disulfonate (AQDS). The electrons flowing to methane in hematite-AQDS, magnetite-AQDS, control, sole-AQDS, and goethite-AQDS groups accounted for 81.24, 77.12, 75.42, 70.55, and 56.32% of the total produced electrons, respectively. Hematite addition significantly accelerated the methane production rate (18.97%) compared with sole-AQDS. Electrochemical investigation showed that AQDS might have its oxidation potential reduced by adsorbing on hematite, which results in an energy band bending for hematite and the formation of a biological capacitor. The biological capacitor's integrated electric field helps with the transfer of electrons from reduced AQDS to anaerobic consortia via bulk hematite. Metagenomic and metaproteomic sequencing analyses revealed that the ferredoxin and Mph-reducing hydrogenase in hematite addition increased by 7.16 and 21.91%, respectively, compared to sole-AQDS addition. Accordingly, this research suggested that AH2QDS may re-transfer electrons to methanogens via the biological capacitor and the membrane's Mph-reducing hydrogenase, thus lowering the HA electron competition.

Electronic Bifurcation: A New Perspective on Fe Bio-Utilization in Anaerobic Digestion of Lactate.

Anaerobic microorganisms use flavin/quinone-based electronic bifurcation (EB) to gain a survival advantage at the thermodynamic limits. However, the contribution of EB to microscopic energy and productivity in the anaerobic digestion (AD) system is unknown. This study demonstrates for the first time that under limited substrate conditions, Fe-driven EB in AD leads to a 40% increase in specific methane production and contributes to 25% ATP accumulation, by analyzing the concentration of EB enzymes such as Etf-Ldh, HdrA2B2C2, and Fd, NADH and actual Gibbs free-energy changes. Differential pulse voltammetry and electron respiratory chain inhibition experiments detected that iron enhanced electron transport in EB by accelerating the activity of flavin, Fe-S clusters, and quinone groups. Other microbial and enzyme genes with EB potential closely related to iron transport have also been found in metagenomes. The potential of EB to accumulate energy and enhance productivity in AD systems was investigated, and metabolic pathways were proposed in the study.

Coexistence of Synechococcus and Microcystis Blooms in a Tropical Urban Reservoir and Their Links with Microbiomes.

Bacteria play a crucial role in driving ecological processes in aquatic ecosystems. Studies have shown that bacteria-cyanobacteria interactions contributed significantly to phytoplankton dynamics. However, information on the contribution of bacterial communities to blooms remains scarce. Here, we tracked changes in the bacterial community during the development of a cyanobacterial bloom in an equatorial estuarine reservoir. Two forms of blooms were observed simultaneously corresponding to the lotic and lentic characteristics of the sampling sites where significant spatial variabilities in physicochemical water quality, cyanobacterial biomass, secondary metabolites, and cyanobacterial/bacterial compositions were detected. Microcystis dominated the upstream sites during peak periods and were succeeded by Synechococcus when the bloom subsided. For the main body of the reservoir, a mixed bloom featuring coccoid and filamentous cyanobacteria (Microcystis, Synechococcus, Planktothricoides, Nodosilinea, Raphidiopsis, and Prochlorothrix) was observed. Concentrations of the picocyanobacteria Synechococcus remained high throughout the study, and their positive correlations with cylindrospermopsin and anatoxin-a suggested that they could produce cyanotoxins, which pose more damaging impacts than previously supposed. Succession of different cyanobacteria (Synechococcus and Microcystis) following changes in nutrient composition and ionic strength was demonstrated. The microbiomes associated with blooms were unique to the dominant cyanobacteria. Generic and specialized bloom biomarkers for the Microcystis and downstream mixed blooms were also identified. Microscillaceae, Chthoniobacteraceae, and Roseomonas were the major heterotrophic bacteria associated with Microcystis bloom, whereas Phycisphaeraceae and Methylacidiphilaceae were the most prominent groups for the Synechococcus bloom. Collectively, bacterial community can be greatly deviated by the geological condition, monsoon season, cyanobacterial density, and dominant cyanobacteria.

Responses of cyanobacterium Microcystis aeruginosa under single and repeated ofloxacin exposure.

Antibiotics are omnipresent and pseudo-persistent in the environment. Yet, their potential ecological risks under repeated exposure, which is more environmentally relevant, are understudied. Therefore, this study used ofloxacin (OFL) as the probe chemical to investigate the toxic effects of different exposure scenarios-single dose of high concentration (4.0 µg/L) and multiple additions of low concentrations-towards the cyanobacterium Microcystis aeruginosa. Flow cytometry was employed to measure a collection of biomarkers, including endpoints related with biomass, single cell properties and physiological status. Results showed that the single dose of the highest OFL level inhibited cellular growth, chl-a content and cell size of M. aeruginosa. In contrast, OFL induced stronger chl-a autofluorescence and higher doses tended to have more remarkable effects. Repeated low OFL doses can more significantly increase the metabolic activity of M. aeruginosa than a single high dose. Viability and cytoplasmic membrane were not affected by OFL exposure. Oxidative stress was observed for the different exposure scenarios, with fluctuating responses. This study demonstrated the different physiological responses of M. aeruginosa under different OFL exposure scenarios, providing novel insights into the toxicity of antibiotics under repeated exposure.

Rapid detection methods and modelling simulations provide new insights into cyanobacteria detection and bloom management in a tropical reservoir.

The increasing occurrence of cyanobacteria blooms is of global concern, and is often associated with environmental and socio-economic problems, such as degenerated ecosystems and aquaculture impairment. The diazotrophic cyanobacterium Raphidiopsis raciborskii (R. raciborskii) grows rapidly in the tropics, and produces the toxin, cylindrospermopsin (CYN), which has harmful effects on aquatic organisms. Thus, to protect water quality and ecosystem, it is essential to have rapid and reliable methods for cyanobacteria and R. raciborskii detection and prediction so that early warning can be provided for management. Molecular assays, such as PCR, real-time quantitative PCR (qPCR), two-step PCR assays are accurate and widely used, but still require several hours from sample preparation to data analysis. In this study, insulated isothermal PCR (iiPCR) assays in conjunction with fast DNA extraction method, were developed and verified as a rapid detection assay in detecting cyanobacteria and R. raciborskii within 50 min, and also with high detection accuracy (98.8%) and the overall high agreement level (98.8%, k = 97.5%)) comparing to conventional qPCR assay. However, the limitation of the iiPCR assay is that it only generates qualitative results. Therefore, the quantified iiPCR assay, named as A-iiPCR, by coupling iiPCR device with fluorescence signal catching and interpretation instrument (Andor spectrometer with Solis spectroscopy software) was developed and verified with in situ environmental samples. The fluorescence intensity decreased accordingly with the drop of DNA concentration until reaching 1.32 ng/µL. Also, Delft 3D modelling was established to simulate R. raciborskii change in predicting spatial and temporal variabilities for reservoir management, as the simulated R. raciborskii concentration was the highest at sampling site 1, as well as temporally highest in April and October, posing as the most high-risk location and time periods for R. raciborskii bloom-forming requiring corresponding governance measures.

What's the cost-effective pattern for rural wastewater treatment?

Inadequate sanitation infrastructure is a global problem that is particularly impacting rural areas. And decentralized wastewater management system is considered as the feasible solution for rural sewage treatment (RuST). However, determining the cost-effective (CE) pattern for decentralized RuST is methodologically challenging because of scarce decision-support tools. In this research, a RuST optimization model (RuST-OM) was developed to gain an insight into the CE pattern of RuST based on the greedy algorithm. This model involves tradeoffs in the economy-of-scale and technology of wastewater treatment system versus the cost and energy consumption of the sewage collection system. The investment associated with the CE pattern for RuST is closely linked to the environmental demand, RuST coverage, topographic complexity, and degree of household dispersion. The cost of the CE pattern falls between the onsite-B and community-based pattern, and this range represents the optimized interval for RuST planning. Nature-based technology is a sustainable alternative for RuST in areas characterized by low or moderate environmental demand. To ensure applicability of the RuST-OM in other countries/regions, built-in datasets (e.g., technology and pipeline design parameters) are designed based on rural area features that can be modified as necessary. This research highlights the utility of the CE pattern for RuST planning, and can serve as a reference for RuST planning around the world.

Phycocyanin-rich Synechococcus dominates the blooms in a tropical estuary lake.

Cyanobacterial blooms challenge the safe water supply in estuary reservoirs. Yet, data are limited for the variation of phytoplankton dynamics during an algal bloom event at refined scales, which is essential for interpreting the formation and cessation of blooms. The present study investigated the biweekly abundances and dynamics of pico- and nano-phytoplankton in a tropical estuary lake following a prolonged bloom event. Flow cytometry analysis resolved eight phenotypically distinct groups of phytoplankton assigned to nano-eukaryotes (nano-EU), pico/nano-eukaryotes (PicoNano-EU), cryptophyte-like cells (CRPTO), Microcystis-like cells (MIC), pico-eukaryotes (Pico-EU) and three groups of Synechococcus-like cells. Total phytoplankton abundance ranged widely from 2.4 × 104 to 2.8 × 106 cells cm-3. The phytoplankton community was dominated by Synechococcus-like cells with high phycocyanin content (SYN-PC). Temporal dynamics of the phytoplankton community was phytoplankton- and site-specific. Peak values were observed for SYN-PC, SYN-PE2 (Synechococcus-like cells with low levels of phycoerythrin) and Pico-EU, while the temporal dynamics of other groups were less pronounced. Redundancy analysis (RDA) showed the importance of turbidity as an abiotic factor in the formation of the current SYN-PC induced blooms, and Spearman correlation analysis suggested a competitive relationship between SYN-PC and Pico-EU.

Potential influence of overwintering benthic algae on water quality.

Overwintering benthic algae not only directly impact drinking water safety, but also affect the algae recruitment in warm spring seasons. Thus, understanding the characteristics of overwintering benthic algae can provide scientific references for formulating preventative strategies of reasonable water resource. However, they have received less attention. In this study, the spatiotemporal variation of benthic algae and their harmful secondary metabolites were studied from autumn to summer in Qingcaosha Reservoir. Benthic algae (picophytobenthos accounting for 55.42%) had a high biomass during overwintering, and the groups of overwintering benthic algae included pico-Cyanobacteria, pico-Cryptomonas, pico-Chlorophyta, pico-Diatoms, Cyanobacteria, Chlorophyta, Cryptomonas and Diatoms, which were consistent with the planktonic algae species in warm seasons. In oligotrophic or mesotrophic water bodies, micronutrients of iron and manganese were key nutrient factors influencing the biomass of benthic algae. Furthermore, picophytobenthos were important potential contributors of harmful secondary metabolites. The content of microcystins, anatoxin-a, geosmin and 2-methylisoborneol in sediment were 15.75 µg/kg·FW, 48.16 µg/kg·FW, 3.91 ng/kg·FW, and 11.76 ng/kg·FW during winter, which had potential to be released into water bodies to impact water quality. These findings indicate that water quality monitoring programs need to consider sediment in winter as a potential source of toxins and preventative measures to prevent excessive proliferation of algae should be implemented in winter.

Heavy metals in a typical city-river-reservoir system of East China: Multi-phase distribution, microbial response and ecological risk.

The rapid construction of artificial reservoirs in metropolises has promoted the emergence of city-river-reservoir systems worldwide. This study investigated the environmental behaviors and risks of heavy metals in the aquatic environment of a typical system composed of main watersheds in Suzhou and Jinze Reservoir in Shanghai. Results shown that Mn, Zn and Cu were the dominant metals detected in multiple phases. Cd, Mn and Zn were mainly presented in exchangeable fraction and exhibited high bioavailability. Great proportion and high mobility of metals were found in suspended particulate matter (SPM), suggesting that SPM can greatly affect metal multi-phase distribution process. Spatially, city system (CiS) exhibited more serious metal pollution and higher ecological risk than river system (RiS) and reservoir system (ReS) owing to the diverse emission sources. CiS and ReS were regarded as critical pollution source and sink, respectively, while RiS was a vital transportation aisle. Microbial community in sediments exhibited evident spatial variation and obviously modified by exchangeable metals and nutrients. In particular, Bacteroidetes and Firmicutes presented significant positive correlations with most exchangeable metals. Risk assessment implied that As, Sb and Ni in water may pose potential carcinogenic risk to human health. Nevertheless, ReS was in a fairly safe state. Hg was the main risk contributor in SPM, while Cu, Zn, Ni and Sb showed moderate risk in sediments. Overall, Hg, Sb and CiS were screened out as priority metals and system, respectively. More attention should be paid to these priority issues to promote the sustainable development of the watershed.

Isolation and Characterization of the First Freshwater Cyanophage Infecting Pseudanabaena.

Cyanobacteria are the major primary producers in both freshwater and marine environments. However, the majority of freshwater cyanophages remain unknown due to the limited number of cyanophage isolates. In this study, we present a novel lytic freshwater cyanophage, PA-SR01, which was isolated from the Singapore Serangoon Reservoir. To our knowledge, this is the first isolate of a cyanophage that has been found to infect the cyanobacterium Pseudanabaena PA-SR01 has a narrow host range, a short latent period, and is chloroform sensitive. Distinct from the majority of cyanophage isolates, PA-SR01 has a tailless morphology. It is a double-stranded DNA virus with a 137,012-bp genome. Functional annotation for the predicted open reading frames (ORFs) of the PA-SR01 genome identified genes with putative functions related to DNA metabolism, structural proteins, lysis, host-derived metabolic genes, and DNA packaging. Out of 166 predicted ORFs, only 17 ORFs have homology with genes with known function. Phylogenetic analysis of the major capsid protein and terminase large subunit further suggests that phage PA-SR01 is evolutionary distinct from known cyanophages. Metagenomics sequence recruitment onto the PA-SR01 genome indicates that PA-SR01 represents a new evolutionary lineage of phage which shares considerable genetic similarities with phage sequences in aquatic environments and could play key ecological roles.IMPORTANCE This study presents the isolation of the very first freshwater cyanophage, PA-SR01, that infects Pseudanabaena, and fills an important knowledge gap on freshwater cyanophages as well as cyanophages infecting Pseudanabaena.

Picophytoplankton identification by flow cytometry and high-throughput sequencing in a clean reservoir.

Understanding picophytoplankton variations that play important roles in the material circulation and energy flow are critical to assessing overall status of waterbody, especially for clean reservoirs which remain a relatively stable community structure and high species diversity due to lower nitrogen and phosphorus nutrients. However, their response to key environmental factors and tightly acting microbial remains poorly understood. Traditional quantification methods are limited, such as chlorophyll-a, turbidity and microscope. There are still many defects with present molecular analysis. In this study, a flow cytometric analysis and high-throughput sequencing combination methodology was developed and tested on clean water from a reservoir, by a monthly dynamic for a vegetative period April-September in 2019 to improve the accuracy of dynamic monitoring for the picophytoplankton system. More species of Pico-Cyanobacteria and Pico-Eukaryotes were discovered. The increased percentage of pigment compounds from 8.2% to 76.3% proves the effective reduce of heterotrophic disturbing and enrichment of target populations. Picophytoplankton that was previously neglected due to their low relative abundance has once again entered the scope of our eyes. Phytoplankton were divided into three categories. The first one was the highly abundant and frequently present taxa, the second one was the low-abundance but highly-transient population, and the third one was the low abundance and stable group. Synechococcus, Emiliania, Tetraselmis and Thalassiosira were dominant picophytoplankton and displayed obvious temporal and spatial distribution characteristics. Pico-PE rich Cyanobacteria and Nano-Eukaryotes with high transience abnormally increased in summer. Temperature, ammonia-N, nitrate-N, turbidity and total nitrogen were most influencing factors, while some picophytoplankton with special physiological structure showed distinct competitive advantages in the microbial community. As for the off-flavor compounds, the concentration of 2-methylisoborneol and geosmin were high even 66.7% and 20.8% of the samples exceeded their olfactory threshold. Chrysochromuina, Planktothrix and Microcystis might be the potential producers.

To centralize or to decentralize? A systematic framework for optimizing rural wastewater treatment planning.

Untreated rural sewage seriously affects the universal access to clean water of rural residents. The lack of decision-support tools in rural sewage treatment (RuST) planning makes it difficult for RuST system to achieve the expected results and is not conducive to the optimal allocation of limited funds. Hence, there is an urgent need to develop a decision-support framework for large-scale RuST planning. For the first time, RuST planning decision-support framework was developed using divide-and-conquer strategy based on rural residents' spatial pattern (RESP) and the optimal pattern of RuST. This framework can be transferred to other countries/regions easily by correcting RESP dataset according to the spatial and environmental characteristics. We confirmed that the variation of RESP made the ideal RuST pattern varied significantly under different topography. And community-based pattern could be the optimal pattern for large-scale RuST planning, when spatial obstacle and RESP were fully considered. The price of onsite sewage treatment facility is the most significant factor for RuST planning. In our selected case, requited onsite facility accounted for 65.51%. For the total investment, the cost of sewer systems accounted for 56.01%, and the average investment in plains, hills, platforms and mountains was 1401, 1803, 1903 and 1859 USD/household, respectively. We expect this research could provide reference for RuST planning in other developing countries/regions all around the world.

Multi-phase distribution, spatiotemporal variation and risk assessment of antibiotics in a typical urban-rural watershed.

The widespread consumption and continuous discharge of antibiotics have threatened the ecological health of urban-rural watershed. In this study, multi-phase distribution, spatiotemporal variation and ecological risk of 18 antibiotics in rivers and lakes from Suzhou City were investigated based on urban-rural gradient. The total antibiotic concentration in surface water, suspended particulate matter (SPM) and sediments was 39.28-2578 ng/L, 6.16-171.09 ng/L and 12.67-2249 ng/g, respectively. High detection frequency (>76%) and concentration of antibiotics in multi-phase suggested universal pollution. Quinolones (QNs) and tetracycline (TCs) were the dominant antibiotics detected. The partitioning coefficient (KP) value of SPM-water was 1.43-29.93 times larger than sediment-water, indicating that SPM can greatly affect the fate and distribution of antibiotics. Significant positive correlations between antibiotics and environmental parameters (e.g. TOC, TP and TN) revealed combined contamination and similar pollution sources. Antibiotic pollution exhibited evident spatiotemporal variation. For spatial variation, urban area showed more serious antibiotic pollution and greater ecological risk than rural and suburb areas, especially for sediments. Besides, antibiotic level and risk in rivers were higher than lakes. For seasonal variation, in case of surface water, rural area exhibited higher content in winter, while greater content was detected in autumn and spring in urban and suburb areas, respectively. The highest antibiotic content in SPM and sediments was all measured in winter owing to weak degradation ability. Ecological risk assessment based on risk quotients (RQs) indicated that norfloxacin (NFX), ciprofloxacin (CFX) and anhydroerythromycin (ETM-H2O) in surface water presented medium to high risk throughout the entire year, while sulfadiazine (SDZ) and enrofloxacin (EFX) in sediments showed higher accumulation potential. Thus, these five antibiotics were selected as the priority antibiotics for pollution control. In short, this study improves the understanding of antibiotic fates in the urban-rural watershed and provides scientific basis for the authorities to regulate antibiotic pollution.

Metabolites change of Scenedesmus obliquus exerted by AgNPs.

With increasing emission of silver nanoparticles (AgNPs) into the environment, it is important to understand the effects of ambient concentration of AgNPs. The biological effects of AgNPs on Scenedesmus obliquus, a ubiquitous freshwater microalgae, was evaluated. AgNPs exerted a minor inhibitory effect at low doses. Non-targeted metabolomic studies were conducted to understand and analyze the effect of AgNPs on algal cells from a molecular perspective. During the 48 hr of exposure to AgNPs, 30 metabolites were identified, of which nine had significant changes compared to the control group. These include d-galactose, sucrose, and d-fructose. These carbohydrates are involved in the synthesis and repair of cell walls. Glycine, an important constituent amino acid of glutathione, increased with AgNP exposure concentration increasing, likely to counteract an increased intracellular oxidative stress. These results provide a new understanding of the toxicity effects and mechanism of AgNPs. These metabolites could be useful biomarkers for future research, employed in the early detection of environmental risk from AgNPs.