RESUMO
To clarify the microvascular changes and the effector sites of lansoprazole during the formation of colitis, the dextran sulfate sodium (DSS)-induced colitis was induced by the oral administration for 3 and 7 days. The alteration of the microvascular permeability was estimated by the intraaortic infusion of FITC-dextran. The effector sites of 3H-lansoprazole were examined by the intraaortic infusion of the radiolabelled compound and the autoradiographic procedure of water-soluble compounds. As a result, marked increase of the microvascular permeability was detected three days after DSS treatment near the inflammatory cells in the tip portion of the colonic mucosa. 3H-lansoprazole in the control rat colon was localized in the goblet cells, while in DSS-treated rats, 3H-lansoprazole was accumulated in the cytoplasm of the mesenchymal cells, and most of them coincided with polymorphonuclear leucocytes and macrophages.
Assuntos
Permeabilidade Capilar/efeitos dos fármacos , Colite/patologia , Omeprazol/análogos & derivados , 2-Piridinilmetilsulfinilbenzimidazóis , Animais , Sítios de Ligação , Colite/induzido quimicamente , Citoplasma/metabolismo , Sulfato de Dextrana , Células Caliciformes/metabolismo , Mucosa Intestinal/patologia , Lansoprazol , Macrófagos , Neutrófilos , Omeprazol/farmacocinética , Inibidores da Bomba de Prótons , Ratos , Ratos WistarRESUMO
We devised and evaluated a clinical pathway (CP) protocol for patients with bleeding peptic ulcers (BPU). Patients without severe comorbidities, who had been diagnosed with BPU and who had undergone endoscopic treatment, were enrolled in our study. The CP adaptation rate for BPU patients was 78.8% (89/113). The variance rate was 13.5% (12/89). The median length of admission was 10.0 +/- 4.6 days (n = 78) before and 7.4 +/- 2.9 days (n = 77) after introducing CP. Our CP for BPU was safe and resulted in shorter hospital stays and, therefore, cost reductions. In elder patients, our CP was also successful, but the variance rate was higher than in younger patients.
Assuntos
Procedimentos Clínicos , Úlcera Péptica Hemorrágica/terapia , Adulto , Idoso , Idoso de 80 Anos ou mais , Feminino , Humanos , Tempo de Internação , Masculino , Pessoa de Meia-Idade , Úlcera Péptica Hemorrágica/economiaRESUMO
To clarify the effector sites of lansoprazole in the colonic mucosa during the formation of colitis, dextran sulfate sodium-induced colitis was induced by the oral administration of 3% aqueous solution for 3 and 7 days. The effector sites of [3H]lansoprazole were examined by the intra-aortic infusion of the radiolabelled compound and the autoradiographic tracing of water-soluble compounds. As a result, the [3H]lansoprazole binding in the control rat colon was negligible, while in dextran sulfate sodium-treated rats specific binding sites of [3H]lansoprazole were recognized in the cytoplasm of the mesenchymal cells, and most of them coincided with polymorphonuclear leucocytes and macrophages.
Assuntos
Colite/metabolismo , Neutrófilos/metabolismo , Omeprazol/análogos & derivados , 2-Piridinilmetilsulfinilbenzimidazóis , Animais , Anti-Infecciosos/administração & dosagem , Anti-Infecciosos/metabolismo , Anti-Infecciosos/farmacologia , Apoptose/efeitos dos fármacos , Autorradiografia/métodos , Sítios de Ligação , Ligação Competitiva , Colite/induzido quimicamente , Colo/efeitos dos fármacos , Colo/patologia , Colo/ultraestrutura , Sulfato de Dextrana , Modelos Animais de Doenças , Fundo Gástrico/efeitos dos fármacos , Fundo Gástrico/patologia , Fundo Gástrico/ultraestrutura , Infusões Intra-Arteriais , Mucosa Intestinal/enzimologia , Mucosa Intestinal/imunologia , Mucosa Intestinal/patologia , Lansoprazol , Macrófagos/efeitos dos fármacos , Macrófagos/patologia , Masculino , Microscopia Eletrônica/métodos , Neutrófilos/efeitos dos fármacos , Neutrófilos/patologia , Omeprazol/metabolismo , Omeprazol/farmacologia , Inibidores da Bomba de Prótons , Ratos , Ratos Wistar , TrítioRESUMO
To determine the effects of rebamipide during the early stages of colitits development, colitis was induced in rats by oral administration of dextran sulfate sodium for 3 or 7 days. The target sites of (3)H-rebamipide were examined by intra-aortic infusion of the radiolabeled compound followed by autoradiography. (3)H-rebamipide was localized in goblet cells in the colon of the control rat, whereas it accumulated in the cytoplasm of mesenchymal cells in dextran sulfate sodium treated rats, localized predominantly to polymorphonuclear leucocytes.