Guardianship dietary and other behavioural factors associated with skipping breakfast in Japanese primary school children: A child-guardian dyad study.

BACKGROUND AND OBJECTIVES: Regular breakfast consumption is widely considered an important component of healthy dietary habits. In this study, we assessed differences in nutrient/food intake between breakfast consumers and skippers. We also investigated behavioural factors related with breakfast skipping utilizing data collected from both children and their guardians. METHODS AND STUDY DESIGN: This cross-sectional study was conducted in 14 public primary schools in Japan. Two questionnaires were distributed: a behavioural questionnaire and a brieftype, self-administered diet history questionnaire. In total, 1816 child (10-12 y/o)-guardian dyads were included in the analysis. Intakes of nutrients and foods were compared between breakfast consumers and skippers by the ttest. The relationship between breakfast skipping and behavioural factors was investigated by logistic regression analysis by child sex. RESULTS: The proportion of breakfast skippers in the children was 9.8%. Daily intakes of nutrients/foods were better in the breakfast consumers. Later bedtime, lower nutrition knowledge level, and frequent incomplete consumption of home meals was significantly or marginally associated with breakfast skipping. Guardians' breakfast skipping was also associated with children's breakfast skipping. Some relationships between behavioural factors and breakfast skipping differed between boys and girls. CONCLUSIONS: Breakfast consumers had a more nutritious meal quality. To increase breakfast consumption, morning time schedules may need to be rearranged to avoid time pressure. Nutritional knowledge and dietary behaviour appear relevant. Guardians played a major role in coping with breakfast avoidance, in part as role models.

Rapid detection and identification of Streptococcus ratti by a species-specific PCR method.

To establish a rapid and species-specific detection and identification method of Streptococcus ratti by polymerase chain reaction, two PCR primer pairs specific to S. ratti were designed on the basis of the nucleotide sequence of the dextranase gene (dex) of S. ratti ATCC19645(T). The primer pairs specifically detected S. ratti, but none of the other mutans streptococci (16 strains of 6 species). The PCR procedure was capable of detecting 1 pg of genomic DNA purified from S. ratti ATCC19645. We developed the Streptococcus mutans-, Streptococcus sobrinus-, Streptococcus downei- and Streptococcus salivarius-specific PCR methods (the dex PCR methods) with the primer pairs specific for a portion of the dex gene of each species. The mixture of these primer pairs including S. ratti (this study) successfully differentiated the five species of mutans streptococci by species-specific amplicons of different lengths. These results suggest that the present PCR method is suitable for the specific detection and identification of S. ratti, and that the mixture of primer pairs for the dex PCR methods is useful for species-specific detection and rapid discrimination of each species in mutans streptococci.