RESUMO
Itch triggers scratching, a behavioural defence mechanism that aids in the removal of harmful irritants and parasites1. Chemical itch is triggered by many endogenous and exogenous cues, such as pro-inflammatory histamine, which is released during an allergic reaction1. Mechanical itch can be triggered by light sensations such as wool fibres or a crawling insect2. In contrast to chemical itch pathways, which have been extensively studied, the mechanisms that underlie the transduction of mechanical itch are largely unknown. Here we show that the mechanically activated ion channel PIEZO1 (ref. 3) is selectively expressed by itch-specific sensory neurons and is required for their mechanically activated currents. Loss of PIEZO1 function in peripheral neurons greatly reduces mechanically evoked scratching behaviours and both acute and chronic itch-evoked sensitization. Finally, mice expressing a gain-of-function Piezo1 allele4 exhibit enhanced mechanical itch behaviours. Our studies reveal the polymodal nature of itch sensory neurons and identify a role for PIEZO1 in the sensation of itch.
Assuntos
Canais Iônicos , Prurido , Alelos , Animais , Canais Iônicos/deficiência , Canais Iônicos/genética , Canais Iônicos/metabolismo , Camundongos , Prurido/genética , Prurido/fisiopatologia , Sensação , Células Receptoras Sensoriais/metabolismoRESUMO
The cornea of the eye differs from other mucosal surfaces in that it lacks a viable bacterial microbiome and by its unusually high density of sensory nerve endings. Here, we explored the role of corneal nerves in preventing bacterial adhesion. Pharmacological and genetic methods were used to inhibit the function of corneal sensory nerves or their associated transient receptor potential cation channels TRPA1 and TRPV1. Impacts on bacterial adhesion, resident immune cells, and epithelial integrity were examined using fluorescent labeling and quantitative confocal imaging. TRPA1/TRPV1 double gene-knockout mice were more susceptible to adhesion of environmental bacteria and to that of deliberately-inoculated Pseudomonas aeruginosa. Supporting the involvement of TRPA1/TRPV1-expressing corneal nerves, P. aeruginosa adhesion was also promoted by treatment with bupivacaine, or ablation of TRPA1/TRPV1-expressing nerves using RTX. Moreover, TRPA1/TRPV1-dependent defense was abolished by enucleation which severs corneal nerves. High-resolution imaging showed normal corneal ultrastructure and surface-labeling by wheat-germ agglutinin for TRPA1/TRPV1 knockout murine corneas, and intact barrier function by absence of fluorescein staining. P. aeruginosa adhering to corneas after perturbation of nerve or TRPA1/TRPV1 function failed to penetrate the surface. Single gene-knockout mice showed roles for both TRPA1 and TRPV1, with TRPA1-/- more susceptible to P. aeruginosa adhesion while TRPV1-/- corneas instead accumulated environmental bacteria. Corneal CD45+/CD11c+ cell responses to P. aeruginosa challenge, previously shown to counter bacterial adhesion, also depended on TRPA1/TRPV1 and sensory nerves. Together, these results demonstrate roles for corneal nerves and TRPA1/TRPV1 in corneal resistance to bacterial adhesion in vivo and suggest that the mechanisms involve resident immune cell populations.
Assuntos
Aderência Bacteriana , Córnea , Pseudomonas aeruginosa/metabolismo , Canal de Cátion TRPA1/metabolismo , Canais de Cátion TRPV/metabolismo , Animais , Córnea/inervação , Córnea/metabolismo , Córnea/microbiologia , Feminino , Masculino , Camundongos , Camundongos Knockout , Canal de Cátion TRPA1/genética , Canais de Cátion TRPV/genéticaRESUMO
Sphingosine-1-phosphate (S1P) plays important roles as a signaling lipid in a variety of physiological and pathophysiological processes. S1P signals via a family of G-protein-coupled receptors (GPCRs) (S1P1-5) and intracellular targets. Here, we report on photoswitchable analogs of S1P and its precursor sphingosine, respectively termed PhotoS1P and PhotoSph. PhotoS1P enables optical control of S1P1-3, shown through electrophysiology and Ca2+ mobilization assays. We evaluated PhotoS1P in vivo, where it reversibly controlled S1P3-dependent pain hypersensitivity in mice. The hypersensitivity induced by PhotoS1P is comparable to that induced by S1P. PhotoS1P is uniquely suited for the study of S1P biology in cultured cells and in vivo because it exhibits prolonged metabolic stability compared to the rapidly metabolized S1P. Using lipid mass spectrometry analysis, we constructed a metabolic map of PhotoS1P and PhotoSph. The formation of these photoswitchable lipids was found to be light dependent, providing a novel approach to optically probe sphingolipid biology.
Assuntos
Lisofosfolipídeos/metabolismo , Esfingosina/análogos & derivados , Animais , Lisofosfolipídeos/química , Camundongos , Modelos Moleculares , Estrutura Molecular , Imagem Óptica , Processos Fotoquímicos , Esfingosina/química , Esfingosina/metabolismoRESUMO
Sphingosine 1-phosphate (S1P) is a bioactive signaling lipid associated with a variety of chronic pain and itch disorders. S1P signaling has been linked to cutaneous pain, but its role in itch has not yet been studied. Here, we find that S1P triggers itch and pain in male mice in a concentration-dependent manner, with low levels triggering acute itch alone and high levels triggering both pain and itch. Ca2+ imaging and electrophysiological experiments revealed that S1P signals via S1P receptor 3 (S1PR3) and TRPA1 in a subset of pruriceptors and via S1PR3 and TRPV1 in a subset of heat nociceptors. Consistent with these findings, S1P-evoked itch behaviors are selectively lost in mice lacking TRPA1, whereas S1P-evoked acute pain and heat hypersensitivity are selectively lost in mice lacking TRPV1. We conclude that S1P acts via different cellular and molecular mechanisms to trigger itch and pain. Our discovery elucidates the diverse roles that S1P signaling plays in somatosensation and provides insight into how itch and pain are discriminated in the periphery.SIGNIFICANCE STATEMENT Itch and pain are major health problems with few effective treatments. Here, we show that the proinflammatory lipid sphingosine 1-phosphate (S1P) and its receptor, S1P receptor 3 (S1PR3), trigger itch and pain behaviors via distinct molecular and cellular mechanisms. Our results provide a detailed understanding of the roles that S1P and S1PR3 play in somatosensation, highlighting their potential as targets for analgesics and antipruritics, and provide new insight into the mechanistic underpinnings of itch versus pain discrimination in the periphery.
Assuntos
Lisofosfolipídeos/metabolismo , Dor/metabolismo , Prurido/metabolismo , Receptores de Lisoesfingolipídeo/metabolismo , Transdução de Sinais/fisiologia , Esfingosina/análogos & derivados , Canais de Cátion TRPV/metabolismo , Animais , Cálcio/metabolismo , Camundongos , Camundongos Knockout , Dor/genética , Prurido/genética , Receptores de Lisoesfingolipídeo/genética , Esfingosina/metabolismo , Receptores de Esfingosina-1-Fosfato , Canais de Cátion TRPV/genéticaRESUMO
ATP-binding cassette (ABC) transporters are evolutionarily conserved proteins that pump diverse substrates across membranes. Many are known to efflux signaling molecules and are extensively expressed during development. However, the role of transporters in moving extracellular signals that regulate embryogenesis is largely unexplored. Here, we show that a mesodermal ABCC (MRP) transporter is necessary for endodermal gut morphogenesis in sea urchin embryos. This transporter, Sp-ABCC5a (C5a), is expressed in pigment cells and their precursors, which are a subset of the non-skeletogenic mesoderm (NSM) cells. C5a expression depends on Delta/Notch signaling from skeletogenic mesoderm and is downstream of Gcm in the aboral NSM gene regulatory network. Long-term imaging of development reveals that C5a knockdown embryos gastrulate, but â¼90% develop a prolapse of the hindgut by the late prism stage (â¼8 h after C5a protein expression normally peaks). Since C5a orthologs efflux cyclic nucleotides, and cAMP-dependent protein kinase (Sp-CAPK/PKA) is expressed in pigment cells, we examined whether C5a could be involved in gastrulation through cAMP transport. Consistent with this hypothesis, membrane-permeable pCPT-cAMP rescues the prolapse phenotype in C5a knockdown embryos, and causes archenteron hyper-invagination in control embryos. In addition, the cAMP-producing enzyme soluble adenylyl cyclase (sAC) is expressed in pigment cells, and its inhibition impairs gastrulation. Together, our data support a model in which C5a transports sAC-derived cAMP from pigment cells to control late invagination of the hindgut. Little is known about the ancestral functions of ABCC5/MRP5 transporters, and this study reveals a novel role for these proteins in mesoderm-endoderm signaling during embryogenesis.
Assuntos
AMP Cíclico/metabolismo , Intestinos/embriologia , Proteínas Associadas à Resistência a Múltiplos Medicamentos/metabolismo , Ouriços-do-Mar/embriologia , Adenilil Ciclases/metabolismo , Animais , Compostos de Boro/química , Proteínas Quinases Dependentes de AMP Cíclico/metabolismo , Embrião não Mamífero/metabolismo , Endoderma/metabolismo , Gástrula/metabolismo , Gastrulação , Regulação da Expressão Gênica no Desenvolvimento , Hibridização in Situ Fluorescente , Mesoderma/metabolismo , Microscopia Confocal , Microscopia de Fluorescência , Morfogênese , Nucleotídeos/química , Fenótipo , Pigmentação , Transdução de SinaisRESUMO
The kidney functions as a finely tuned sensor to balance body fluid composition and filter out waste through complex coordinated mechanisms. This versatility requires tight neural control, with innervating efferent nerves playing a crucial role in regulating blood flow, glomerular filtration rate, water and sodium reabsorption, and renin release. In turn sensory afferents provide feedback to the central nervous system for the modulation of cardiovascular function. However, the cells targeted by sensory afferents and the physiological sensing mechanisms remain poorly characterized. Moreover, how the kidney is innervated during development to establish these functions remains elusive. Here, we utilized a combination of light-sheet and confocal microscopy to generate anatomical maps of kidney sensory and sympathetic nerves throughout development and resolve the establishment of functional crosstalk. Our analyses revealed that kidney innervation initiates at embryonic day (E)13.5 as the nerves associate with vascular smooth muscle cells and follow arterial differentiation. By E17.5 axonal projections associate with kidney structures such as glomeruli and tubules and the network continues to expand postnatally. These nerves are synapsin I-positive, highlighting ongoing axonogenesis and the potential for functional crosstalk. We show that sensory and sympathetic nerves innervate the kidney concomitantly and classify the sensory fibers as calcitonin gene related peptide (CGRP)+, substance P+, TRPV1+, and PIEZO2+, establishing the presence of PIEZO2 mechanosensory fibers in the kidney. Using retrograde tracing, we identified the primary dorsal root ganglia, T10-L2, from which PIEZO2+ sensory afferents project to the kidney. Taken together our findings elucidate the temporality of kidney innervation and resolve the identity of kidney sympathetic and sensory nerves.
RESUMO
The peripheral somatosensory system bestows mammals with a diverse repertoire of sensory modalities: gentle touch, mechanical pain, itch, thermosensation, and proprioception. The cells and molecules that transduce many of these stimuli have already been characterized. But how somatosensory neurons transduce acutely painful mechanical forces is largely unknown and remains one of the 'final frontiers' of sensory neurobiology. In an effort to fill this gap in knowledge, recent studies have identified subpopulations of mechanical pain neurons and uncovered novel modulators of mechanical pain. These studies have greatly advanced our understanding of how noxious mechanical stimuli are detected in mammals. Here, we discuss recent progress in noxious mechanosensation and highlight new behavioral methods to assess mechanical pain.
Assuntos
Dor , Percepção do Tato , Animais , NeurôniosRESUMO
In this issue of Neuron, Royal et al. (2018) find that a mutant form of the TRESK ion channel linked to migraine undergoes alternative translation to produce an inhibitory protein that blocks TREK channels, leading to neuronal hyperexcitability and migraine in rodents.
Assuntos
Transtornos de Enxaqueca , Canais de Potássio/genética , Humanos , Mutação , NeurôniosRESUMO
Sea urchin gametes have been historically used to demonstrate fertilization and early development in student laboratories. Large amounts of egg and sperm are easily acquired, and the conspicuous changes in egg surface morphology, indicative of sperm-egg fusion and egg activation, are readily observed in the classroom. However, less often incorporated into teaching labs are exercises that demonstrate the dramatic metabolic changes that accompany egg activation. One example is the massive up-regulation of various essential transport activities in the embryo's plasma membrane, including xenobiotic transporter activity. Here we outline a laboratory that incorporates this concept into a teaching lab, capitalizing on the magnitude and uniformity of the xenobiotic transporter activation event in certain species of sea urchins. The introduction of this chapter provides background information for the instructor, and the remainder serves as a laboratory manual for students. The experiments detailed within the manual can be completed in a total of 4-8h spread over one or two lab periods. The lab manual guides students through a modified version of the United States Environmental Protection Agency (EPA) toxicity test, a novel undergraduate-level laboratory on xenobiotic transporters, and analysis of microscope data using ImageJ. We have found this lab to be of interest to a wide range of biology and environmental science undergraduates, and effective in teaching underlying concepts in developmental biology, physiology and toxicology.
Assuntos
Fertilização/efeitos dos fármacos , Ouriços-do-Mar/efeitos dos fármacos , Xenobióticos/administração & dosagem , Animais , Transporte Biológico/efeitos dos fármacos , Membrana Celular/metabolismo , Biologia do Desenvolvimento/métodos , Embrião de Mamíferos/efeitos dos fármacos , Células Germinativas/efeitos dos fármacosRESUMO
Chronic itch remains a highly prevalent disorder with limited treatment options. Most chronic itch diseases are thought to be driven by both the nervous and immune systems, but the fundamental molecular and cellular interactions that trigger the development of itch and the acute-to-chronic itch transition remain unknown. Here, we show that skin-infiltrating neutrophils are key initiators of itch in atopic dermatitis, the most prevalent chronic itch disorder. Neutrophil depletion significantly attenuated itch-evoked scratching in a mouse model of atopic dermatitis. Neutrophils were also required for several key hallmarks of chronic itch, including skin hyperinnervation, enhanced expression of itch signaling molecules, and upregulation of inflammatory cytokines, activity-induced genes, and markers of neuropathic itch. Finally, we demonstrate that neutrophils are required for induction of CXCL10, a ligand of the CXCR3 receptor that promotes itch via activation of sensory neurons, and we find that that CXCR3 antagonism attenuates chronic itch.
Assuntos
Dermatite Atópica/imunologia , Neutrófilos/imunologia , Prurido/imunologia , Receptores CXCR3/imunologia , Pele/imunologia , Animais , Calcitriol/administração & dosagem , Calcitriol/análogos & derivados , Linhagem Celular , Quimiocina CXCL10/genética , Quimiocina CXCL10/imunologia , Quimiocina CXCL10/metabolismo , Citocinas/genética , Citocinas/imunologia , Citocinas/metabolismo , Dermatite Atópica/induzido quimicamente , Dermatite Atópica/genética , Modelos Animais de Doenças , Perfilação da Expressão Gênica/métodos , Regulação da Expressão Gênica/efeitos dos fármacos , Regulação da Expressão Gênica/imunologia , Humanos , Queratinócitos/imunologia , Queratinócitos/metabolismo , Camundongos Endogâmicos C57BL , Neutrófilos/metabolismo , Prurido/induzido quimicamente , Prurido/genética , Receptores CXCR3/genética , Receptores CXCR3/metabolismo , Células Receptoras Sensoriais/imunologia , Células Receptoras Sensoriais/metabolismo , Pele/inervação , Pele/metabolismoRESUMO
Somatosensory neurons mediate responses to diverse mechanical stimuli, from innocuous touch to noxious pain. While recent studies have identified distinct populations of A mechanonociceptors (AMs) that are required for mechanical pain, the molecular underpinnings of mechanonociception remain unknown. Here, we show that the bioactive lipid sphingosine 1-phosphate (S1P) and S1P Receptor 3 (S1PR3) are critical regulators of acute mechanonociception. Genetic or pharmacological ablation of S1PR3, or blockade of S1P production, significantly impaired the behavioral response to noxious mechanical stimuli, with no effect on responses to innocuous touch or thermal stimuli. These effects are mediated by fast-conducting A mechanonociceptors, which displayed a significant decrease in mechanosensitivity in S1PR3 mutant mice. We show that S1PR3 signaling tunes mechanonociceptor excitability via modulation of KCNQ2/3 channels. Our findings define a new role for S1PR3 in regulating neuronal excitability and establish the importance of S1P/S1PR3 signaling in the setting of mechanical pain thresholds.
Assuntos
Lisofosfolipídeos/fisiologia , Mecanorreceptores/fisiologia , Dor/fisiopatologia , Transdução de Sinais/fisiologia , Esfingosina/análogos & derivados , Animais , Células Cultivadas , Gânglios Espinais/citologia , Gânglios Espinais/metabolismo , Gânglios Espinais/fisiologia , Canal de Potássio KCNQ2/metabolismo , Canal de Potássio KCNQ2/fisiologia , Canal de Potássio KCNQ3/metabolismo , Canal de Potássio KCNQ3/fisiologia , Camundongos Endogâmicos C57BL , Camundongos Knockout , Camundongos Transgênicos , Limiar da Dor , Receptores de Lisoesfingolipídeo/genética , Receptores de Lisoesfingolipídeo/metabolismo , Esfingosina/fisiologia , Receptores de Esfingosina-1-FosfatoRESUMO
Steroids regulate cell proliferation, tissue development, and cell signaling via two pathways: a nuclear receptor mechanism and genome-independent signaling. Sperm activation, egg maturation, and steroid-induced anesthesia are executed via the latter pathway, the key components of which remain unknown. Here, we present characterization of the human sperm progesterone receptor that is conveyed by the orphan enzyme α/ß hydrolase domain-containing protein 2 (ABHD2). We show that ABHD2 is highly expressed in spermatozoa, binds progesterone, and acts as a progesterone-dependent lipid hydrolase by depleting the endocannabinoid 2-arachidonoylglycerol (2AG) from plasma membrane. The 2AG inhibits the sperm calcium channel (CatSper), and its removal leads to calcium influx via CatSper and ensures sperm activation. This study reveals that progesterone-activated endocannabinoid depletion by ABHD2 is a general mechanism by which progesterone exerts its genome-independent action and primes sperm for fertilization.