RESUMO
We investigated the influence of windows on performance, mood, and satisfaction for different task types. Forty students worked computational or managerial tasks in offices with or without windows. Contrary to expectation, windowed offices did not effect higher performance, positive mood, or satisfaction. Actually, students felt slightly more confident (p < .10) and more in control (p < .01) in the windowless condition, suggesting a need for privacy to reduce evaluation apprehension. How windows affect performance, mood, and satisfaction remains unclear.
Assuntos
Afeto , Atenção , Eficiência , Satisfação no Emprego , Iluminação , Meio Social , Adulto , Feminino , Humanos , Individualidade , Liderança , Masculino , Resolução de ProblemasRESUMO
We have developed a simple and quick method ("wick blot") for detecting the presence of specific DNA sequences in plants, using radiolabeled DNA probes. The method requires only small amounts of tissue, about 15-25 mg. More than a hundred samples per day can be easily extracted and blotted. It works well on various species and tissues, including leaves, embryos, and callus. The method is ideally suited for screening large numbers of putative transformants, especially populations that have not been screened by prior selection.
Assuntos
DNA/análise , Técnicas de Sonda Molecular , Plantas/genética , Sequência de Bases , Sondas de DNA , Hibridização de Ácido Nucleico , Plantas Tóxicas , Nicotiana/genética , Transformação GenéticaRESUMO
The developmental regulation of the translational elongation factor EF-1 alpha has been analyzed in tobacco. A gene fusion was constructed consisting of the 5' and 3' regions of the tomato genomic clone LeEF-A from the EF-1 alpha gene family and the beta-glucuronidase coding region. Analysis of the transgenic plants containing this chimeric gene demonstrated that the tomato LeEF-A flanking sequences were sufficient to confer expression patterns similar to those of the endogenous tobacco EF-1 alpha gene. The patterns of beta-glucuronidase activity in this system indicated that during plant growth and development EF-1 alpha is regulated with increased expression corresponding to regions of high protein synthesis, including meristems, rapidly growing tissues, and developing gametophytes. In addition, EF-1 alpha expression responds rapidly to changes in growth patterns induced by hormone treatment. Our results are in agreement with studies in animals indicating that EF-1 alpha expression may be rate limiting for protein synthesis and demonstrate that the analysis of EF-1 alpha is of value for studying interrelationships between protein synthesis and developmental control.