Evaluating the effects of surfactant types on the properties and stability of oil-in-water Rhodiola rosea nanoemulsion.

Different types and ratios of surfactant, co-surfactant, and oil phase, have a greater impact on nanoemulsion preparation. The presence of surfactants in the nanoemulsion can reduce surface tension and characteristic stability. In this study, four groups of oil-in-water (O/W) nanoemulsions (NEs) with different ratios of surfactant and co-surfactant, and two oils were formulated as carriers of Rhodiola rosea. The variable optimization was investigated and then indicated as optimization group A (Opt A) with the formula of 10% of transcutol, 16.63% of tween 80, Opt B with 10% of tween 80, 29.87% of span 80, Opt C with 28.42% of transcutol, 30% of labrasol, and Opt D with 30% of transcutol, 30% of tween 80. Labrafac and soybean oil were used as the oil phase. The optimized formula using the response surface method (RSM) by design expert software showed the ideal conditions with a higher desirability score. Desirability score are 0.72% (Opt A), 0.81% (Opt B), 0.76% (Opt C) and 0.98% (Opt D), the desirability rating close to 1 indicates a high possibility that the projected values would closely match the experimental results for the optimum formula. All of the optimized formulation were also checked for the characteristics of nanoemulsion including particle size, polydispersity index (PDI), zeta potential, viscosity, encapsulation efficiency, transmission electron microscope (TEM), antioxidant activity, skin irritation test and stability studies. Our study provides a promising combination of surfactant-co-surfactant and oil phases to produce a stable nanoemulsion that can be used in pharmaceuticals and cosmetics in the future.

6-Octadecenoic and Oleic Acid in Liquid Smoke Rice Husk Showed COVID-19 Inhibitor Properties.

In recent years, liquid smoke rice husk (LSRH) has shown its therapeutic potency to diabetes, wound healing, stomatitis, and periodontitis. The phenol, 6-octadecenoic acid, oleic acid, and 9-octadecanoic acid were responsible for their therapeutic effect. The LSRH also demonstrated their potential for infectious diseases such as coronavirus disease (COVID-19). Therefore, the molecular dynamics (MDs) simulation and pharmacophore analysis was performed to analyse the binding stability of 6-octadecenoic and oleic acid. Based on MD simulation, 6-octadecenoic and oleic acids seemed to retain their interactions with Ser144 and Thr24, respectively, with hydrogen bond distance less than 2.9 Å. This interaction was stable during the simulation and has hydrophobic and hydrogen bonds/acceptors. The 6-octadecenoic acid and oleic acid were confirmed to have great potency as inhibitors for COVID-19. These compounds also showed that the existence of hydrophobic and hydrogen bonds/acceptors could increase biological activity.

Dynamic changes in the metabolome and microbiome during Citrus depressa Hayata liquid fermentation.

Citri Reticulatae Pericarpium (CRP) is a common traditional Chinese herbal medicine, valued for its multi-bioactivity. However, its processing time, environment, and microorganisms all affect its quality and bioactivity. To address this, the study replaced solid-state fermentation with liquid fermentation using microorganisms and isolated Bacillus amyloliquefaciens, respectively. This aimed to discover a more stable processing method and examine metabolite-micobiota correlations. Non-targeted metabolomics identified 70 differential metabolites, focusing on amino acids, polymethoxyflavones (PMFs), and carbohydrates. Long-read sequencing showed a shift in dominant bacterial genera from Lactobacillus to Pediococcus, then to Clostridium. Spearman analysis revealed a positive correlation between specific Clostridium species and PMFs production. B. amyloliquefaciens fermentation notably increased PMFs content without reducing hesperidin levels, suggesting its potential as an alternative processing method. This study offers valuable insights into metabolome-microbiome interactions for future biotransformation research.

In Silico Study of Liquid Smoke Rice Husk against COVID-19.

OBJECTIVES: Liquid smoke rice husk has been researched and proved to consist of antibacterial, analgesic, anti-inflammatory, and growth factor stimulatory properties. By these complex properties, the liquid smoke rice hull is able to purpose as a novel coronavirus disease 2019 (COVID-19) inhibitor. The research was conducted to analyze the role of the dominant compound in rice husk liquid smoke against one of the main proteases in complex with inhibitor N3 of COVID-19 and 6LU7 protein data bank (PDB) ID. MATERIAL: AND METHODS: The Molecular Operating Environment (MOE) 2020.0901 (Chemical computing group) was used to analyzed the interaction. The molecular structure test, including phenol, mequinol, 2-methoxy-phenol, 6-octadecenoic acid, oleic acid, 9-cctadecenoic acid, was chosen. The lopinavir as positive control and 6LU7 as COVID-19 protein were chosen. All the protein analyses were conducted using docking molecular. RESULT: The phenol, 2-methoxy-phenol, mequinol and 9-octadecenoic acid have higher binding free energy that causes difficult to bind to the active site of protein 6LU7 (-3.4758, -3.5509, -3.6845, and -5.0173 kcal/mol, respectively). The minor component of liquid smoke, such as 6-octadecenoic acid and oleic acid, has the binding free energy (-5,5327 and -5,4208 kcal/mol) and more factor of binding presumably as active COVID-19 inhibitor. CONCLUSION: The liquid smoke rice husk has active component like 6-octadecenoic acid and oleic acid are presumably as active COVID-19 inhibitor.

Nanoparticles of Liquid Smoke Rice Husk Inhibit Porphyromonas gingivalis.

OBJECTIVE: Utilization of liquid smoke rice husk can be used as an alternative treatment because of the antimicrobial properties. Advances in drug delivery systems are increasingly developing to increase the bioavailability of drugs and reduce the side effects of these drugs, namely nanoparticles. In this study, nanoparticles of liquid smoke rice husk (nLSRH) were tested the antimicrobial against Porphyromonas gingivalis. MATERIALS AND METHOD: This type of research is an experimental in vitro laboratory using Porphyromonas gingivalis culture. nLSRH contained liquid smoke rice husk concentration of 1, 2.5, 5, 7.5, 10, 12.5, 15, and 17.5%. The antibacterial was performed using the dilution methods. RESULTS: The nLRSH concentration of 1% showed clearest medium. The highest number of colonies Porphyromonas gingivalis was observed at nLSRH concentration of 1% (40.3 colony-forming unit [CFU]) and decreased at a concentration of 2.5% (11.3 CFU); other concentration or no bacterial colony growth was found. The nLSRH concentration of 2.5% can be determined as the minimum inhibitory concentration and nLSRH concentration of 5% can be determined as the minimum bactericidal concentration. CONCLUSION: nLSRH have antimicrobial activity against Porphyromonas gingivalis. This finding able to drive the next research to develop nLSRH as gingival and periodontitis disease is caused by Porphyromonas gingivalis.

Osteoblast Viability of Liquid Smoke Rice Hull and Nanoparticles Form as Periodontitis Treatment.

OBJECTIVE: Rice husk liquid smoke nanoparticles have the potential to be developed as a drug because they have anti-inflammatory effects that can modulate the process of osteoblast stimulation through osteoblast stimulation by thorough small size and enter cells easily. The osteoblast is the key of alveolar regeneration in periodontitis treatment. This present study analyzed the differences of liquid smoke rice husk and nanoparticles of liquid smoke rice husk on osteoblast viability as periodontitis treatment MATERIALS AND METHODS: The liquid smoke rice husk was obtained from the pyrolysis process. The nanoparticles were made with chitosan, maltodextrin, and difference of concentration of liquid smoke rice husk (such as 1, 2.5, 5, 7.5, 10, 12.5, 15, and 17.5%). The viability of osteoblast was analyzed by 2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. STATISTICAL ANALYSIS: The data were analyzed using independent t-test to analyze the differences between liquid smoke rice husk and nanoparticles of liquid smoke rice husk, the significant was set a p<0.05. RESULT: The nanoparticles of liquid smoke rice husk showed higher osteoblast viability compared liquid smoke rice husk. The nanoparticles' concentration of 5, 7.5, and 10% showed higher osteoblast viability compared liquid smoke rice husk (p = 0.002, 0.000, and 0.001, respectively). CONCLUSION: The nanoparticles of liquid smoke rice husk showed higher viability of osteoblast. This confirmed that the nanoparticles were able to reduce the toxicity in the higher concentration of liquid smoke of rice husk.

Formulation, characteristics and anti-bacterial effects of Euphorbia hirta L. mouthwash.

Objective: Mouthwash is a liquid solution used to improve oral health and breath freshness as well as reduce oral bacteria. This study aims to formulate a Euphorbia hirta L. ethanol extract for mouthwash, evaluate the physical properties, and determine its anti-bacterial effects against Streptococcus mutans. Methods: Each mouthwash formula was created by utilising a solubilisation technique. Three mouthwash formulas were created from different concentrations of Euphorbia hirta L. ethanol extract (0.5%, 1%, and 2%), and referred to as F1, F2, and F3. The organoleptic properties, pH levels, specific gravity, viscosity, flow properties, stability, irritation level, contact time, and anti-bacterial effects were evaluated for each concentration. Result: The resulting formulas featured a distinctive smell of oleum menthae piperitae and had a sweet and spicy taste. These characteristics remained unaffected during storage. The acidity levels ranged from 4.59 to 6.0, the weight masses ranged from 0.9693 to 1.0710 g/ml, and the viscosity ranged from 1.50 to 3.00 cP. F3 concentration was non-irritating with mucus production at 11.325%, had lower contact time with a neutralisation of 57.14% of Streptococcus mutans colonies in 30 seconds, and showed stable anti-bacterial control against Streptococcus mutans (p = 0.000) within the first week of use. Conclusion: The study results offer the first proof of Euphorbia hirta L. used for improving human health. The formulation features the ideal physical and stability characteristics of mouthwash, and possesses anti-bacterial properties that can potentially combat Streptococcus mutans. Clinical use of the formulated mouthwash must be explored in future research.