RESUMO
Oocysts obtained from a guanaco and an alpaca with natural infections were identified as Eimeria macusaniensis and evaluated for host specificity and infectivity over time. In 3 separate trials conducted over 4 yr, 4 adult llamas were fed 500-5,000 sporulated oocysts obtained from guanaco feces stored under laboratory conditions for 41-84 mo. Infections with prepatent periods of 36-41 days and patent periods of 38-55 days developed in 4/4 llamas. In a fourth trial, 3 adult llamas and 1 alpaca were each fed 1,000 sporulated E. macusaniensis oocysts obtained from alpaca feces stored in the laboratory for 3 mo. Infections with prepatent periods of 33-34 days and patent periods of 14-20 days developed in 3/3 llamas. Infection in the alpaca had a prepatent period of 58 days and a patent period of 1 day. Clinical signs associated with infection, if any, were minimal and included increased fecal mucus and occasional soft feces. These results provide evidence that E. macusaniensis is a single species transmissible amongst alpacas, llamas, and guanacos and that oocysts of this species can remain infective for many years.
Assuntos
Camelídeos Americanos/parasitologia , Coccidiose/veterinária , Eimeria/patogenicidade , Fezes/parasitologia , Animais , Coccidiose/parasitologia , Masculino , Oocistos/fisiologia , Especificidade da Espécie , Esporos de Protozoários/patogenicidade , Fatores de TempoRESUMO
Feces containing Isospora suis oocysts were collected from naturally- and experimentally-infected pigs from four different areas of the United States. The unsporulated oocysts were cleaned, concentrated, mixed with 2.5% aqueous potassium dichromate solution, poured into petri dishes to a depth of 5 mm, and incubated at 25 degrees C. The oocysts were examined with a microscope at 12 h intervals and the stages of sporulation present were counted. Although a few oocysts were completely sporulated after 12 h of incubation, in most fecal samples the majority of the oocysts were not completely sporulated until 24 or 36 h. In the present study, the sporulation time of I. suis oocysts was considered to be less than or equal to 48 h. There were no major differences in the sporulation times of I. suis oocysts from the different sources.
Assuntos
Fezes/parasitologia , Isospora/fisiologia , Animais , Esporos/fisiologia , Suínos , Fatores de Tempo , Estados UnidosRESUMO
To compare the prevalence of Eimeria macusaniensis among midwestern llamas (Lama glama), alpacas (Lama pacos), and guanacos (Lama guanicoe), feces were obtained from Lama spp. in 10 states between October 1989 and February 1996. Feces were examined by centrifugal flotation in sugar solution (specific gravity--1.28-1.30), and oocysts were quantified by a modified McMaster method. Data were compared by host species and age classifications. Typical oocysts occurred in samples from 28% of 76 herds and 10.4% of 443 animals including 12% of 301 llamas, 7% of 115 alpacas, and 7.4% of 27 guanacos. Prevalence was significantly greater (P = 0.009) in animals < 1 yr of age in comparison to older animals for llams (22.1 v.s. 8.5%) and for all Lama spp. combined (17.1 vs. 8.4%). Fecal oocyst abundance was significantly greater (P = 0.001) in llamas < 1 yr of age in comparison to older llamas (30 vs. 16 oocysts per g of feces). Fecal oocyst intensities did not differ significantly. Prevalence in both age groups of midwestern llamas was greater than previously reported for llamas in the western United States. Prevalence in midwestern alpacas < 1 yr of age was lower than reported for alpacas of similar age in South America, but oocyst intensities were similar. These results indicate that infection with E. macusaniensis is more common in Lama spp. in North America than previously recognized.
Assuntos
Camelídeos Americanos/parasitologia , Coccidiose/veterinária , Eimeria/isolamento & purificação , Animais , Coccidiose/epidemiologia , Fezes/parasitologia , Meio-Oeste dos Estados Unidos/epidemiologia , Contagem de Ovos de Parasitas , PrevalênciaRESUMO
Heads of hunter-killed white-tailed deer (Odocoileus virginianus) were obtained from a tri-county area in central Iowa in December 1991 and examined for the presence of Parelaphostrongylus tenuis (Dougherty, 1945). Adults of P. tenuis were found in 45% of 42 deer heads; the mean intensity of infection was 3.6 (range: 1-16). Prevalence of infection was significantly greater in female deer > 13 mo of age in comparison to males of similar age (54 vs. 17%). Mean intensity of infection was significantly greater in deer > 13 mo of age than in younger deer (4.4 vs. 1.2 nematodes/head). Both prevalence and intensity of P. tenuis infection in deer in Iowa were comparable to levels reported elsewhere in the United States and Canada. These results further define the distribution of P. tenuis and suggest that susceptible hosts sharing habitat with white-tailed deer in Iowa are at risk of infection with this parasite.
Assuntos
Cervos/parasitologia , Metastrongyloidea/isolamento & purificação , Infecções por Strongylida/veterinária , Animais , Dura-Máter/parasitologia , Feminino , Iowa , Masculino , Meninges/parasitologia , Prevalência , Infecções por Strongylida/epidemiologia , Infecções por Strongylida/parasitologiaRESUMO
Two pregnant llamas (Lama glama) infected with Toxoplasma gondii and their offspring were evaluated clinically and serologically. Llama 1 was inoculated orally with 1,000 infective occysts of the P89 strain of T. gondii at 82 days of gestation (DOG). Llama 2 became naturally infected with T. gondii between 26 and 119 DOG. Both llamas remained clinically normal and delivered healthy offspring. Sera collected from both llamas during pregnancy and from their offspring before and after colostral ingestion were evaluated for antibodies to T. gondii by the modified agglutination test (MAT), latex agglutination test (LAT), indirect hemagglutination test (IHAT), and the Sabin-Feldman dye test (DT). In llama 1, MAT antibody titers were < 1:20, 1:320, 1:1,280, 1:640, and 1:80 at 82, 97, 109, 132, and 152 DOG, respectively. The MAT titers in naturally infected llama 2 were < 1:32, 1:320-1:640, and 1:1,280 at 26, 119-200, and 346 DOG, respectively. In both llamas, antibody titers in the DT were of similar magnitude as the MAT, but titers in the LAT and IHAT were inconsistent. Antibodies to T. gondii were not detected in precolostral sera obtained from offspring of both llamas suggesting there was no fetal T. gondii infection.
Assuntos
Anticorpos Antiprotozoários/sangue , Camelídeos Americanos/parasitologia , Complicações Parasitárias na Gravidez/veterinária , Toxoplasma/imunologia , Toxoplasmose Animal/imunologia , Testes de Aglutinação/veterinária , Animais , Anticorpos Antiprotozoários/análise , Colostro/imunologia , Feminino , Testes de Hemaglutinação/veterinária , Imunidade Materno-Adquirida , Transmissão Vertical de Doenças Infecciosas/veterinária , Testes de Fixação do Látex/veterinária , Gravidez , Complicações Parasitárias na Gravidez/diagnóstico , Complicações Parasitárias na Gravidez/imunologia , Toxoplasmose Animal/diagnóstico , Toxoplasmose Animal/transmissãoRESUMO
Serum proteins were evaluated by agarose electrophoresis at periodic intervals between postinoculation days (PID) 2 and 35 in 4 litters of pigs inoculated orally at 36 to 38 hours of age with 70,000 sporulated Isospora suis oocysts and in 4 litters of age-matched noninoculated control pigs. In inoculated pigs, clinical disease characterized by vomiting and diarrhea began at PID 3 to 4 and was resolved by PID 11. Isospora suis oocysts were detected in feces of inoculated pigs from PID 5 to 25, with peak numbers present on PID 5 to 6. Of 43 pigs inoculated with I suis oocysts, 4(9.3%) died of coccidiosis, and samples were not obtained for serum protein evaluation. Of 39 noninoculated control pigs, all remained clinically normal, and I suis oocysts were not detected in their feces. Serum protein fraction values in inoculated and control groups compared at each sample collection time did not differ significantly, except at PID 15, when beta-globulin values were lower in inoculated pigs (P = 0.02). At PID 35, total serum proteins, albumin, and alpha 1-, beta-, and gamma-globulin values of inoculated pigs were lower than those of controls, but there were too few pigs examined for definitive statistical analysis. Differences in trends over time were observed between inoculated and control groups for several serum protein fractions. In inoculated pigs, total proteins (P less than 0.1) and beta-globulins (P less than 0.01) decreased with time, whereas those of control pigs increased. Similar differences in trends were noticed for albumin and alpha 1-globulins, but these were not significant.(ABSTRACT TRUNCATED AT 250 WORDS)
Assuntos
Proteínas Sanguíneas/análise , Coccidiose/veterinária , Doenças dos Suínos/sangue , Doença Aguda , Animais , Animais Recém-Nascidos , Coccidiose/sangue , Eletroforese em Gel de Ágar , Fezes/parasitologia , Feminino , Isospora/isolamento & purificação , Masculino , SuínosRESUMO
Twenty Holstein steers subclinically infected with coccidia were allotted to 2 groups of 10 steers each. One group received a diet containing 0.5 mg of decoquinate/kg of body weight. After 25 days on the diet, there was no difference between the groups in lymphocyte blastogenic responsiveness to mitogens; however, there were differences in neutrophil function. Lymphocytes from steers of the decoquinate-fed group had decreased random migration under agarose, enhanced cytochrome C reduction, and enhanced iodination activity. Other measures of neutrophil function evaluated (chemotactic index, Staphylococcus aureus ingestion, and antibody-dependent and -independent cell-mediated cytotoxicity) were not affected. After 30 days of decoquinate feeding, half of the cattle in each group received 5 daily IM injections of dexamethasone (0.04 mg/kg of body weight). The dexamethasone-treated steers from the group that did not have decoquinate in the diet developed clinical coccidiosis, whereas the decoquinate-treated steers remained clinically normal. Lymphocyte and neutrophil function were again evaluated for a 3-day period beginning 4 days after dexamethasone treatment was halted. Neutrophils from the steers that developed clinical coccidiosis after dexamethasone administration had significantly (P less than 0.05) inhibited random migration under agarose, cytochrome C reduction, and iodination activity, but significantly (P less than 0.01) enhanced S aureus ingestion. The feeding of decoquinate prevented the inhibition of neutrophil cytochrome C reduction and lessened the inhibition of neutrophil iodination in the dexamethasone-treated group. Dexamethasone treatment was associated with an inhibition of lymphocyte blastogenic responsiveness to phytohemagglutinin in principals as well as controls.
Assuntos
Doenças dos Bovinos/imunologia , Coccidiose/veterinária , Decoquinato/farmacologia , Dexametasona/farmacologia , Hidroxiquinolinas/farmacologia , Neutrófilos/fisiologia , Animais , Bovinos , Quimiotaxia de Leucócito/efeitos dos fármacos , Coccidiose/imunologia , Contagem de Leucócitos/veterinária , Ativação Linfocitária/efeitos dos fármacos , Masculino , Neutrófilos/imunologia , FagocitoseRESUMO
The pharmacokinetic behaviour of ivermectin was investigated in adult llamas (Lama glama) by using high performance liquid chromatography with a lower limit of quantification of 2 ng/ml to measure its concentration in serum. Llamas were treated with one of three commercial formulations (injectable, pour-on or oral paste) at dosages recommended by the manufacturer, or with an experimental injectable sustained-release formulation. In five llamas given 1 per cent ivermectin subcutaneously at 200 microg/kg, the median peak serum concentration (Cmax) was 3 ng/ml and the area under the serum concentration-time curve (AUC) was 13.5 ng x day/ml. In six llamas treated topically with 0.5 per cent ivermedin pour-on at 500 microg/kg, Cmax was 2.5 ng/ml or less and the AUC was 7.75 ng x day/ml or less. In seven llamas with measurable concentrations of ivermedin, the median times to peak serum concentration (tmax) were six days after subcutaneous injection and seven days after treatment with the pour-on formulation. In six llamas, the serum concentration of ivermectin remained less than 2 ng/ml for 124 hours after treatment with a 1.87 per cent oral paste at 200 microg/kg. In five llamas treated subcutaneously with 25 per cent ivermectin sustained-release microspheres at 1500 microg/kg, the median Cmax was 5 ng/ml and the median AUC was 224 ng x day/ml.
Assuntos
Anti-Helmínticos/farmacocinética , Ivermectina/farmacocinética , Animais , Anti-Helmínticos/sangue , Área Sob a Curva , Camelídeos Americanos , Cromatografia Líquida de Alta Pressão , Preparações de Ação Retardada , Feminino , Injeções Subcutâneas , Ivermectina/sangue , MasculinoRESUMO
The course of infection with trypomastigotes of Trypanosoma cruzi (House 510 strain) in mice and guinea pigs with genetic complement deficiencies was compared with that in normocomplementemic animals. Parasitemias in a mouse strain (B10.D2/old) genetically deficient in C5 and therefore unable to sustain lysis were similar to or lower than in a congenic normocomplementemic strain (B10.D2/new). The levels of C3 measured immunochemically were generally unaffected. There were no significant differences in mortality rates. These results indicate that, in mice, complement-mediated lysis does not play a significant role in the control of T. cruzi (House 510) infections. Studies were also performed in normocomplementemic guinea pigs and in guinea pigs genetically deficient in the fourth component of complement and thus unable to support functions mediated by the classical pathway of complement activation. No significant differences were noted between the two strains in the course of infection, persistence of subpatent infection, or rate of mortality, indicating that if the classical complement pathway plays a role in resistance to T. cruzi (House 510) in guinea pigs, this role must be a small one.
Assuntos
Doença de Chagas/imunologia , Complemento C4/genética , Complemento C5/genética , Cobaias/genética , Camundongos/genética , Animais , Masculino , Músculos/patologia , Miocárdio/patologiaRESUMO
The role of complement in host resistance to infection with Trypanosoma lewisi was studied in normal, C4-deficient, and C3-depleted rats. Complement levels were measured in normal rats throughout the course of infection. A drastic reduction of total complement and C4 hemolytic activities occurred, and C3 levels measured immunochemically were decreased. Although total complement and C4 levels were regularly reduced to less than 10% of preinfection levels regardless of parasite numbers, the degree of C3 consumption correlated with the parasitemia. C3 levels varied from 100% of preinfection in rats with light infections to 35% in animals with heavy parasitemias. Recovery to normal levels followed trypanosome elimination from the peripheral blood. The infection had no significant effect on C6 hemolytic activity. Parasitemias and C3 levels in C4-deficient rats did not differ from those of normocomplementemic controls. Depletion of C3 and late-acting components by cobra venom factor during the reproductive or adult stages of infection did not alter the parasitemias. In addition, T. lewisi and immune serum caused complement activation in vitro, which could be inhibited with ethylene glycol-bis-(beta-aminoethyl ether)N,N'-tetraacetic acid or ethylenediaminetetraacetic acid. It is concluded that T. lewisi infection in rats result in activation of the classical complement pathway with extensive consumption of the early-acting components, as well as a low degree of activation of the alternative pathway. However, complement does not appear to play a major role in the control and termination of the infection.
Assuntos
Proteínas do Sistema Complemento/metabolismo , Trypanosoma lewisi/imunologia , Animais , Complemento C3/metabolismo , Complemento C4/deficiência , Complemento C4/metabolismo , Ácido Edético/farmacologia , Ácido Egtázico/farmacologia , Ratos , Venenos de Serpentes/farmacologia , Tripanossomíase/imunologiaRESUMO
The role of complement in host resistance to infection with Trypanosoma musculi was studied in normal, C5-deficient, and C3-depleted mice. Infections in normocomplementemic strains (CBA and B10.D2/n) were generally similar to those in strains genetically deficient in C5 (A and B10.D2/o). There were no differences in inhibition of reproduction, duration of infection, persistence of parasites in the kidneys, or resistance to reinfection. However, peak parasitemias in B10.D2/o mice were slightly greater than in B10.D2/n mice. In addition, B10.D2/o mice had slightly decreased serum levels of C1 early in the course of infection and of C3 early during the elimination of adult forms. These components were unchanged or increased in infections of B10.D2/n. Depletion of C3 and late-acting components in B10.D2/n mice by treatment with cobra venom factor during the reproductive stage of infection resulted in an increase of reproductive forms before the apparent development of ablastic immunity as well as slightly greater peak parasitemias when compared with those of untreated controls. Cobra venom factor treatment of B10.D2/o mice during the reproductive stage did not alter the course of infection. Cobra venom factor treatment of C3H mice during the adult stage prolonged infections by interfering with parasite elimination. It is concluded that complement-mediated lysis is not involved in control of T. musculi. It is not clear whether a C3-dependent function such as phagocytosis may facilitate elimination of the parasites. The major difference in degree of parasitemias among the various strains of mice studied is due to genetic factors rather than the levels of C3, C5, or late-acting complement components.