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1.
Structure ; 7(8): 891-902, 1999 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-10467146

RESUMO

BACKGROUND: Glutamate mutase (Glm) equilibrates (S)-glutamate with (2S,3S)-3-methylaspartate. Catalysis proceeds with the homolytic cleavage of the organometallic bond of the cofactor to yield a 5'-desoxyadenosyl radical. This radical then abstracts a hydrogen atom from the protein-bound substrate to initiate the rearrangement reaction. Glm from Clostridium cochlearium is a heterotetrameric molecule consisting of two sigma and two epsilon polypeptide chains. RESULTS: We have determined the crystal structures of inactive recombinant Glm reconstituted with either cyanocobalamin or methylcobalamin. The molecule shows close similarity to the structure of methylmalonyl CoA mutase (MCM), despite poor sequence similarity between its catalytic epsilon subunit and the corresponding TIM-barrel domain of MCM. Each of the two independent B12 cofactor molecules is associated with a substrate-binding site, which was found to be occupied by a (2S,3S)-tartrate ion. A 1:1 mixture of cofactors with cobalt in oxidation states II and III was observed in both crystal structures of inactive Glm. CONCLUSIONS: The long axial cobalt-nitrogen bond first observed in the structure of MCM appears to result from a contribution of the species without upper ligand. The tight binding of the tartrate ion conforms to the requirements of tight control of the reactive intermediates and suggests how the enzyme might use the substrate-binding energy to initiate cleavage of the cobalt-carbon bond. The cofactor does not appear to have a participating role during the radical rearrangement reaction.


Assuntos
Clostridium/enzimologia , Cobamidas/metabolismo , Transferases Intramoleculares/química , Cobalto/química , Transferases Intramoleculares/metabolismo , Modelos Moleculares , Ligação Proteica , Conformação Proteica , Relação Estrutura-Atividade , Tartaratos/metabolismo
2.
J Mol Biol ; 310(1): 271-80, 2001 Jun 29.
Artigo em Inglês | MEDLINE | ID: mdl-11419952

RESUMO

Product release is partially rate determining in the isomerization reaction catalyzed by Triosephosphate Isomerase, the conversion of dihydroxyacetone phosphate to D-glyceraldehyde 3-phosphate, probably because an active-site loop movement is necessary to free the product from confinement in the active-site. The timescale of the catalytic loop motion and of ligand release were studied using 19F and 31P solution-state NMR. A 5'-fluorotryptophan was incorporated in the loop N-terminal hinge as a reporter of loop motion timescale. Crystallographic studies confirmed that the structure of the fluorinated enzyme is indistinguishable from the wild-type; the fluorine accepts a hydrogen bond from water and not from a protein residue, with minimal perturbation to the flexible loop stability. Two distinct loop conformations were observed by 19F NMR. Both for unligated (empty) and ligated enzyme samples a single species was detected, but the chemical shifts of these two distinct species differed by 1.2 ppm. For samples in the presence of subsaturating amounts of a substrate analogue, glycerol 3-phosphate, both NMR peaks were present, with broadened lineshapes at 0 degrees C. In contrast, a single NMR peak representing a rapid average of the two species was observed at 30 degrees C. We conclude that the rate of loop motion is less than 1400 s(-1) at 0 degrees C and more than 1400 s(-1) at 30 degrees C. Ligand release was studied under similar sample conditions, using 31P NMR of the phosphate group of the substrate analogue. The rate of ligand release is less than 1000 s(-1) at 0 degrees C and more than 1000 s(-1) at 30 degrees C. Therefore, loop motion and product release are probably concerted and likely to represent a rate limiting step for chemistry.


Assuntos
Espectroscopia de Ressonância Magnética , Triose-Fosfato Isomerase/química , Triose-Fosfato Isomerase/metabolismo , Triptofano/análogos & derivados , Sítios de Ligação , Catálise , Cristalografia por Raios X , Glicerofosfatos/metabolismo , Ligação de Hidrogênio , Cinética , Ligantes , Modelos Moleculares , Movimento (Física) , Mutação , Maleabilidade , Conformação Proteica , Saccharomyces cerevisiae/enzimologia , Saccharomyces cerevisiae/genética , Soluções , Triose-Fosfato Isomerase/genética , Triptofano/química , Triptofano/metabolismo
3.
J Synchrotron Radiat ; 7(Pt 4): 267-73, 2000 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-16609206

RESUMO

Crystal structures of several proteins with a B(12) cofactor show abnormally long axial bonds between the cofactor's Co atom and its ;lower' ligand, which is typically a protein-derived imidazole from a histidine residue. X-ray absorption spectroscopy (XAS) experiments were carried out with the following cofactor derivatives to examine the question of whether the bond elongation might be due to an X-ray-induced reduction of the cofactor's cobalt centre: aquocobalamin, cyanocobalamin, methylcobalamin, 5'-desoxyadenosylcobalamin and cob(II)alamin. Each cofactor was investigated at 100 K in a water/glycerol or water/trehalose glass, both as unbound free species and bound to the protein components of the enzyme glutamate mutase. XAS data were collected for each sample around the cobalt absorption edge before and after exhaustive (10 min) irradiation with X-rays of energy 7.76 keV. While XAS spectra for cob(II)alamin, methylcobalamin and 5'-desoxyadenosylcobalamin were the same (within experimental error) before and after irradiation, both in the free and protein-bound state, the spectra of samples with aquocobalamin and cyanocobalamin changed substantially upon irradiation. The spectra of the irradiated samples resembled each other and were similar - but not identical - to the spectrum of the reduced cob(II)alamin. The implications of these observations for the interpretation of the ;long' axial Co-N bonds observed crystallographically in B(12) proteins are discussed.

4.
Acta Crystallogr D Biol Crystallogr ; 57(Pt 8): 1127-34, 2001 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-11468396

RESUMO

The combined molecular-replacement protocol uses a limited six-dimensional search to solve a structure by the molecular-replacement method, with the sampling of the rotational degrees of freedom guided by the rotation function. This protocol therefore automatically combines the information on the rotational and translational parameters of the search model. The combined molecular-replacement protocol has been implemented in a new computer program, COMO. The calculation of the Patterson correlation translation function has been optimized to improve its speed performance. A packing check is implemented that automatically removes impossible solutions and thereby increases the signal in the calculation. A family of atomic models can be used as the search model; the program will automatically select the model that gives the best result. The command interface is well organized and requires the definition of only a few critical parameters by the user. In addition, a graphical user interface has been constructed for the program. The program has been used to solve several difficult molecular-replacement problems. A case is presented where the program automatically determined the orientation and position of five copies of a search model in a high-symmetry space group.


Assuntos
Simulação por Computador , Software , Modelos Teóricos , Solubilidade , Interface Usuário-Computador
5.
Acta Crystallogr D Biol Crystallogr ; 54(Pt 5): 1039-42, 1998 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-9757132

RESUMO

Glutamate mutase [varepsilon2sigma2(B12)1] was reconstituted by incubating purified components E (varepsilon2) and S (sigma2) from Clostridium cochlearium, both produced in Escherichia coli, with either aquo- or cyanocobalamin. The inactive glutamate mutase obtained was crystallized with polyethyleneglycol 4000 as precipitant. Crystals are monoclinic with space group P21 and have cell dimensions a = 64.6, b = 113.2, c = 108.4 A and beta = 96.0 degrees for the glutamate mutase reconstituted with aquocobalamin. They diffract to a resolution of at least 2.7 A. Isolated component S was crystallized in the presence of an excess of cyanocobalamin, yielding red crystals of space group I422 with unit-cell dimensions of a = b = 69.9 and c = 107.1 A. The crystals diffract to about 3.2 A resolution. Native data sets were collected for both crystal forms.


Assuntos
Proteínas de Bactérias/química , Clostridium/enzimologia , Transferases Intramoleculares/química , Conformação Proteica , Proteínas de Bactérias/isolamento & purificação , Cristalização , Cristalografia por Raios X , Transferases Intramoleculares/isolamento & purificação , Proteínas Recombinantes de Fusão/química , Proteínas Recombinantes de Fusão/isolamento & purificação
6.
Acta Crystallogr D Biol Crystallogr ; 55(Pt 1): 51-9, 1999 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-10089394

RESUMO

Using a recently designed neutron single-crystal diffractometer utilizing a narrow-band Laue concept (LADI), diffraction data were collected from a crystal of the coenzyme cob(II)alamin (B12r), crystallized from a mixture of D2O and perdeuterated acetone. The instrument was placed at the end of a cold neutron guide at the Institute Laue Langevin (ILL, Grenoble, France), and data collection with neutrons of 1.8-8.0 A wavelength to a crystallographic resolution of 1.43 A was complete after about 36 h. This compares favourably with a previous experiment utilizing the same crystal specimen, where more than four weeks were required to collect monochromatic diffraction data to about 1 A resolution. Using the Laue data, the structure was solved by molecular replacement with the known X-ray crystal structure. Difference density maps revealed the atomic positions (including deuterium atoms) of seven ordered solvent water molecules and two (partially disordered) acetone molecules. These density maps were compared with corresponding maps computed with monochromatic neutron-diffraction data collected to 1. 0 A resolution using the same crystal specimen, as well as to maps derived from high-resolution (0.90 A) synchrotron X-ray data. In spite of the better definition of atomic positions in the two high-resolution maps, the 1.43 A LADI maps show considerable power for the determination of the location of hydrogen and deuterium positions.


Assuntos
Coenzimas/química , Vitamina B 12/análogos & derivados , Cristalografia por Raios X/métodos , Deutério/química , Hidrogênio/química , Ligação de Hidrogênio , Modelos Moleculares , Nêutrons , Solventes , Vitamina B 12/química
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