Efficient ultra-broadband low-resolution astrophotonic spectrographs.

Broadband low-resolution near-infrared spectrographs in a compact form are crucial for ground- and space-based astronomy and other fields of sensing. Astronomical spectroscopy poses stringent requirements including high efficiency, broad band operation (> 300 nm), and in some cases, polarization insensitivity. We present and compare experimental results from the design, fabrication, and characterization of broadband (1200 - 1650 nm) arrayed waveguide grating (AWG) spectrographs built using the two most promising low-loss platforms - Si3N4 (rectangular waveguides) and doped-SiO2 (square waveguides). These AWGs have a resolving power (λ/Δλ) of ∼200, free spectral range of ∼ 200-350 nm, and a small footprint of ∼ 50-100 mm2. The peak overall (fiber-chip-fiber) efficiency of the doped-SiO2 AWG was ∼ 79% (1 dB), and it exhibited a negligible polarization-dependent shift compared to the channel spacing. For Si3N4 AWGs, the peak overall efficiency in TE mode was ∼ 50% (3 dB), and the main loss component was found to be fiber-to-chip coupling losses. These broadband AWGs are key to enabling compact integrations such as multi-object spectrographs or dispersion back-ends for other astrophotonic devices such as photonic lanterns or nulling interferometers.

High genetic diversity of Anaplasma ovis in sheep from Bosnia and Herzegovina.

BACKGROUND: Ovine anaplasmosis (sensu stricto) is a rickettsial blood disease caused by the tick-borne species Anaplasma ovis. The disease is characterized by mild anemia, fever, and icterus. A more severe clinical presentation is possible in non-endemic areas. There is no existing data on the presence of Anaplasma ovis in Bosnia and Herzegovina. However, given the country's location within the Mediterranean Basin and the recent molecular detection of Babesia ovis, it is plausible that sheep in the region could naturally be infected with this tick-borne pathogen. METHODS AND RESULTS: Blood samples from 81 sheep in the Podrinje and Herzegovina areas were examined by PCR. PCR positivity was found in 38 (46.9%) cases indicating a high number of infected sheep. Mixed infections with Babesia ovis and A.ovis were observed in 63.3% of cases. A higher number of positive sheep was recorded in the area of Herzegovina. Phylogenetic analysis of the gltA, groEL, and msp4 genes of A. ovis revealed numerous genotypes and significant genetic variability. This diversity was not related to geographic origin, tick-borne infection status, or sheep breeding practices in Podrinje and Herzegovina. CONCLUSIONS: The data obtained in this study suggest that the emergence of new genotypes and the high genetic variability of A. ovis are driven by specific local and micro-environmental factors.

Flattening laser frequency comb spectra with a high dynamic range, broadband spectral shaper on-a-chip.

Spectral shaping is critical to many fields of science. In astronomy for example, the detection of exoplanets via the Doppler effect hinges on the ability to calibrate a high resolution spectrograph. Laser frequency combs can be used for this, but the wildly varying intensity across the spectrum can make it impossible to optimally utilize the entire comb, leading to a reduced overall precision of calibration. To circumvent this, astronomical applications of laser frequency combs rely on a bulk optic setup which can flatten the output spectrum before sending it to the spectrograph. Such flatteners require complex and expensive optical elements like spatial light modulators and have non-negligible bench top footprints. Here we present an alternative in the form of an all-photonic spectral shaper that can be used to flatten the spectrum of a laser frequency comb. The device consists of a circuit etched into a silicon nitride wafer that supports an arrayed-waveguide grating to disperse the light over hundreds of nanometers in wavelength, followed by Mach-Zehnder interferometers to control the amplitude of each channel, thermo-optic phase modulators to phase the channels and a second arrayed-waveguide grating to recombine the spectrum. The demonstrator device operates from 1400 to 1800 nm (covering the astronomical H band), with twenty 20 nm wide channels. The device allows for nearly 40 dBs of dynamic modulation of the spectrum via the Mach-Zehnders , which is greater than that offered by most spatial light modulators. With a smooth spectrum light source (superluminescent diode), we reduced the static spectral variation to ∼3 dB, limited by the properties of the components used in the circuit. On a laser frequency comb which had strong spectral modulations, and some at high spatial frequencies, we nevertheless managed to reduce the modulation to ∼5 dBs, sufficient for astronomical applications. The size of the device is of the order of a US quarter, significantly cheaper than their bulk optic counter parts and will be beneficial to any area of science that requires spectral shaping over a broad range, with high dynamic range, including exoplanet detection.

Potential of commercial SiN MPW platforms for developing mid/high-resolution integrated photonic spectrographs for astronomy.

Integrated photonic spectrographs offer an avenue to extreme miniaturization of astronomical instruments, which would greatly benefit extremely large telescopes and future space missions. These devices first require optimization for astronomical applications, which includes design, fabrication, and field testing. Given the high costs of photonic fabrication, multi-project wafer (MPW) silicon nitride (SiN) offerings, where a user purchases a portion of a wafer, provide a convenient and affordable avenue to develop this technology. In this work, we study the potential of two commonly used SiN waveguide geometries by MPW foundries, i.e., square and rectangular profiles, to determine how they affect the performance of mid/high-resolution arrayed waveguide grating (AWG) spectrometers around 1.5 µm. Specifically, we present results from detailed simulations on the mode sizes, shapes, and polarization properties, and on the impact of phase errors on the throughput and cross talk as well as some laboratory results of coupling and propagation losses. From the MPW run tolerances and our phase-error study, we estimate that an AWG with R ∼10,000 can be developed with the MPW runs, and even greater resolving power is achievable with more reliable, dedicated fabrication runs. Depending on the fabrication and design optimizations, it is possible to achieve throughputs ∼60% using the SiN platform. Thus, we show that SiN MPW offerings are highly promising and will play a key role in integrated photonic spectrograph developments for astronomy.

Building hybridized 28-baseline pupil-remapping photonic interferometers for future high-resolution imaging.

One key advantage of single-mode photonic technologies for interferometric use is their ability to easily scale to an ever-increasing number of inputs without a major increase in the overall device size, compared to traditional bulk optics. This is particularly important for the upcoming extremely large telescope (ELT) generation of telescopes currently under construction. We demonstrate the fabrication and characterization of a hybridized photonic interferometer, with eight simultaneous inputs, forming 28 baselines, which is the largest amount to date, to the best of our knowledge. Using different photonic fabrication technologies, we combine a 3D pupil remapper with a planar eight-port ABCD pairwise beam combiner, along with the injection optics necessary for telescope use, into a single integrated monolithic device. We successfully realized a combined device called Dragonfly, which demonstrates a raw instrumental closure-phase stability down to 0.9° over $8\pi$ phase piston error, relating to a detection contrast of ${\sim}6.5 \times {10^{- 4}}$ on an adaptive-optics-corrected 8 m telescope. This prototype successfully demonstrates advanced hybridization and packaging techniques necessary for on-sky use for high-contrast detection at small inner working angles, ideally complementing what can currently be achieved using coronagraphs.

3D-M3: high-spatial-resolution spectroscopy with extreme AO and 3D-printed micro-lenslets.

By combining integral field spectroscopy with extreme adaptive optics, we are now able to resolve objects close to the diffraction limit of large telescopes, exploring new science cases. We introduce an integral field unit designed to couple light with a minimal plate scale from the SCExAO facility at NIR wavelengths to a single-mode spectrograph. The integral field unit has a 3D-printed micro-lens array on top of a custom single-mode multi-core fiber, to optimize the coupling of light into the fiber cores. We demonstrate the potential of the instrument via initial results from the first on-sky runs at the 8.2 m Subaru Telescope with a spectrograph using off-the-shelf optics, allowing for rapid development with low cost.

Vortex fiber nulling for exoplanet observations. I. Experimental demonstration in monochromatic light.

Vortex fiber nulling is a method for spectroscopically characterizing exoplanets at small angular separations, ≲λ/D, from their host star. The starlight is suppressed by creating an optical vortex in the system point spread function, which prevents the stellar field from coupling into the fundamental mode of a single-mode optical fiber. Light from the planet, on the other hand, couples into the fiber and is routed to a spectrograph. Using a prototype vortex fiber nuller (VFN) designed for monochromatic light, we demonstrate coupling fractions of 6×10-5 and >0.1 for the star and planet, respectively.

Quantitative PCR assessment of Lotmaria passim in Apis mellifera colonies co-infected naturally with Nosema ceranae.

A recently described trypanosomatid species Lotmaria passim and the microsporidium Nosema ceranae infect the honey bee (Apis mellifera), but the interspecific dynamic of these two common gut parasites is unknown. In this study, a real-time qPCR assay was developed to enable the specific detection and quantification of L. passim. The annual dynamics of N. ceranae and L. passim infections were evaluated in ten A. mellifera colonies naturally infected with both parasites at one apiary in Serbia from March 2016 to March 2017. Ten samples (60 bees abdomens) were taken from each colony on 8 sampling occasions. L. passim infection level was evaluated with qPCR, while N. ceranae infection was measured by spore counts. N. ceranae infection level was significantly higher in comparison with that of L. passim (spore or cell equivalents/bee, respectively). Significant positive correlation between infection levels of the parasite species indicates their similar annual dynamics, whilst the differences in the levels of infection between particular months point to a seasonal pattern in the incidence of both parasites. The assay which has been developed and validated creates opportunity for detailed study of L. passim infection kinetics and the improvement in the management practices in beekeeping related to these two parasites.

Low loss mid-infrared ZBLAN waveguides for future astronomical applications.

Photonic technologies will be at the heart of future terrestrial planet hunting interferometers. In particular the mid-infrared spectral region between 3.5 - 4.2 µm is the ideal window for hunting for young extra-solar planets, since the planet is still hot from its formation and thus offers a favorable contrast with respect to the parent star compared to other spectral regions. This paper demonstrates two basic photonic building blocks of such an instrument, namely single-mode waveguides with propagation losses as low as 0.29±0.03 dB/cm at a wavelength of 4 µm as well as directional couplers with a constant splitting ratio across a broad wavelength band of 500 nm. The devices are based on depressed cladding waveguides inscribed into ZBLAN glass using the femtosecond laser direct-write technique. This demonstration is the first stepping stone towards the realization of a high transmission mid-infrared nulling interferometer.

High-performance 3D waveguide architecture for astronomical pupil-remapping interferometry.

The detection and characterization of extra-solar planets is a major theme driving modern astronomy. Direct imaging of exoplanets allows access to a parameter space complementary to other detection methods, and potentially the characterization of exoplanetary atmospheres and surfaces. However achieving the required levels of performance with direct imaging from ground-based telescopes (subject to Earth's turbulent atmosphere) has been extremely challenging. Here we demonstrate a new generation of photonic pupil-remapping devices which build upon the Dragonfly instrument, a high contrast waveguide-based interferometer. This new generation overcomes problems caused by interference from unguided light and low throughput. Closure phase measurement scatter of only ∼ 0.2° has been achieved, with waveguide throughputs of > 70%. This translates to a maximum contrast-ratio sensitivity between star and planet at 1λ/D (1σ detection) of 5.3 × 10(-4) (with a conventional adaptive-optics system) or 1.8 × 10(-4) (with 'extreme-AO'), improving even further when random error is minimized by averaging over multiple exposures. This is an order of magnitude beyond conventional pupil-segmenting interferometry techniques (such as aperture masking), allowing a previously inaccessible part of the star to planet contrast-separation parameter space to be explored.

Prevalence of Risk Factors in Patients with Postprocedural Ischemic Lesions after Coiling of Very Small Intracranial Aneurysms.

Background: Very small intracranial aneurysms, generally considered to be those 3 mm in diameter or smaller, pose particular technical challenges for endovascular surgeons. For this reason, very small aneurysms have been excluded from many relevant studies. The aim of our research was to establish the risk factors for the occurrence of stroke complications after endovascular embolization of ruptured and unruptured small intracranial aneurysms. Methods: During the period of 2009-2023, our team performed endovascular embolizations of intracranial aneurysms in 1567 patients across four different centers within the territory of Serbia and Montenegro. Within the total number of patients mentioned, aneurysms of less than 4 mm were treated 185 times, with 119 ruptured and 66 unruptured. Results: In the group of 119 patients with ruptured small intracranial aneurysms, 19 (16%) patients had ischemia after the endovascular treatment, 6 (5%) patients had minor neurological deficits, while 13 (10.9%) patients had major neurological deficits, of which 6 (5%) patients died. In the group of 66 patients with unruptured small intracranial aneurysms, 7 (10.6%) patients had ischemia after the endovascular treatment, 5 (7.6%) patients had minor neurological deficits, and 2 (3.03%) had major neurological deficits. Multivariate binary logistic regression showed that the risk factors for the occurrence of ischemia were the patient's age, smoking and alcohol consumption. The type of endovascular treatment used also had a statistically significant effect on the development of ischemia. Conclusions: Understanding the influence of possible risk factors for the occurrence of ischemic insult after embolization of small intracranial aneurysms is of great importance. By recognizing them, periprocedural complications can be reduced to a minimum.

Gastrointestinal Parasites in Owned Dogs in Serbia: Prevalence and Risk Factors.

Dogs are the most popular pets worldwide. Close contact between dogs and people increases the risk of transmission of various zoonotic parasitic infections. Given the importance of veterinary medicine in preserving the One Health concept, the aim of this research was to identify intestinal parasites that may have zoonotic potential and to evaluate risk factors (individual and environmental). The research was conducted in Serbia in 2022 and 2023 on 382 owned dogs, using qualitative methods of coprological examination with a concentration on parasitic elements. The overall prevalence of intestinal parasites was 62.6%, with the following detected: protozoa: Cystoisospora spp. (9.2%), Sarcocystis spp. (4.5%), Neospora caninum/Hammondia spp. (3.7%), Giardia intestinalis (11.8%); nematoda: Toxocara canis (11.5%), Toxascaris leonina (4.2%), family Ancylostomatidae (38.0%), Trichuris vulpis (21.5%), Capillaria spp. (10.5%); trematoda: Alaria alata (1.6%) and cestodes from the Taeniidae family (1.3%). Factors like age, size and coat length, as well as the way of living, attitude and diet were linked to a significantly higher (p < 0.05) prevalence of intestinal parasites. Based on the results of coprological diagnostics, this research indicates the importance of educating dog owners, conducting routine parasitological tests on their pets and regular deworming strategies.

Potential of Wormwood and Oak Bark-Based Supplement in Health Improvement of Nosema ceranae-Infected Honey Bees.

Nosema ceranae, a microsporidian parasite, as one of the stressors that contribute to honey bee decline, has a significant negative impact on the longevity, productivity, and reproductive capacity of honey bee colonies. There are several different strategies for Nosema infection control, including natural-based and antibiotic-based products. In this study, we tested wormwood and oak bark-based supplement "Medenko forte" on survival, Nosema infection, oxidative stress, and expression of immune-related genes in artificially N. ceranae-infected bees. The results revealed a positive influence on the survival of Nosema-infected bees, irrespectively of the moment of supplement application (day 1, day 3, or day 6 after bee emergence), as well as reduction of Nosema loads and, consequently, Nosema-induced oxidative stress. Supplementation had no negative effects on bee immunity, but better anti-Nosema than immune-stimulating effects were affirmed based on expression levels of abaecin, defensin, hymenoptaecin, apidaecin, and vitellogenin genes. In conclusion, the tested supplement "Medenko forte" has great potential in the health protection of Nosema-infected bees. However, further investigations need to be performed to elucidate its mechanisms of action.

Integrated photonic building blocks for next-generation astronomical instrumentation II: the multimode to single mode transition.

There are numerous advantages to exploiting diffraction-limited instrumentation at astronomical observatories, which include smaller footprints, less mechanical and thermal instabilities and high levels of performance. To realize such instrumentation it is imperative to convert the atmospheric seeing-limited signal that is captured by the telescope into a diffraction-limited signal. This process can be achieved photonically by using a mode reformatting device known as a photonic lantern that performs a multimode to single-mode transition. With the aim of developing an optimized integrated photonic lantern, we undertook a systematic parameter scan of devices fabricated by the femtosecond laser direct-write technique. The devices were designed for operation around 1.55 µm. The devices showed (coupling and transition) losses of less than 5% for F/# ≥ 12 injection and the total device throughput (including substrate absorption) as high as 75-80%. Such devices show great promise for future use in astronomy.

Theoretical modeling and experiments on a DBR waveguide laser fabricated by the femtosecond laser direct-write technique.

We present a model for a Yb-doped distributed Bragg reflector (DBR) waveguide laser fabricated in phosphate glass using the femtosecond laser direct-write technique. The model gives emphasis to transverse integrals to investigate the energy distribution in a homogenously doped glass, which is an important feature of femtosecond laser inscribed waveguide lasers (WGLs). The model was validated with experiments comparing a DBR WGL and a fiber laser, and then used to study the influence of distributed rare earth dopants on the performance of such lasers. Approximately 15% of the pump power was absorbed by the doped "cladding" in the femtosecond laser inscribed Yb doped WGL case with the length of 9.8 mm. Finally, we used the model to determine the parameters that optimize the laser output such as the waveguide length, output coupler reflectivity and refractive index contrast.

Low bend loss waveguides enable compact, efficient 3D photonic chips.

We present a novel method to fabricate low bend loss femtosecond-laser written waveguides that exploits the differential thermal stabilities of laser induced refractive index modifications. The technique consists of a two-step process; the first involves fabricating large multimode waveguides, while the second step consists of a thermal post-annealing process, which erases the outer ring of the refractive index profile, enabling single mode operation in the C-band. By using this procedure we report waveguides with sharp bends (down to 16.6 mm radius) and high (80%) normalized throughputs. This procedure was used to fabricate an efficient 3D, photonic device known as a "pupil-remapper" with negligible bend losses for the first time. The process will also allow for complex chips, based on 10's - 100's of waveguides to be realized in a compact foot print with short fabrication times.

Effects of Plant-Based Supplement on Oxidative Stress of Honey Bees (Apis mellifera) Infected with Nosema ceranae.

One of the most important approaches in the prevention and treatment of nosemosis is the use of herbal preparations as food supplements for bees. Therefore, the aim of this study was to investigate the effects of a plant-based supplement branded as "B+" on honeybees in a laboratory experiment. Four experimental groups were established: treated group (T), N. ceranae-infected and treated group (IT), N. ceranae-infected group (I) and non-infected group (NI). Survival, N. ceranae spore load and oxidative stress parameters together with expression levels of antioxidant enzyme genes and vitellogenin gene were monitored. The mortality in the T, IT and NI groups was significantly (p < 0.001) lower than in than in the I group. Within Nosema-infected groups, the IT group had a significantly lower (p < 0.001) number of N. ceranae spores than the I group. In addition, expression levels of genes for antioxidant enzymes were lower (p < 0.001) in the IT group compared to the I group. The concentration of malondialdehyde and the activities of antioxidant enzymes (superoxide dismutase, catalase and glutathione S-transferase) were significantly lower (p < 0.001) in the IT group compared to the I group. No negative effects of the tested supplement were observed. All these findings indicate that the tested supplement exerted beneficial effects manifested in better bee survival, reduced N. ceranae spore number and reduced oxidative stress of bees (lower expression of genes for antioxidant enzymes and oxidative stress parameters).

Direct imaging and astrometric detection of a gas giant planet orbiting an accelerating star.

Direct imaging of gas giant exoplanets provides information on their atmospheres and the architectures of planetary systems. However, few planets have been detected in blind surveys with direct imaging. Using astrometry from the Gaia and Hipparcos spacecraft, we identified dynamical evidence for a gas giant planet around the nearby star HIP 99770. We confirmed the detection of this planet with direct imaging using the Subaru Coronagraphic Extreme Adaptive Optics instrument. The planet, HIP 99770 b, orbits 17 astronomical units from its host star, receiving an amount of light similar to that reaching Jupiter. Its dynamical mass is 13.9 to 16.1 Jupiter masses. The planet-to-star mass ratio [(7 to 8) × 10-3] is similar to that of other directly imaged planets. The planet's atmospheric spectrum indicates an older, less cloudy analog of the previously imaged exoplanets around HR 8799.

Developing arrayed waveguide grating spectrographs for multi-object astronomical spectroscopy.

With the aim of utilizing arrayed waveguide gratings for multi-object spectroscopy in the field of astronomy, we outline several ways in which standard telecommunications grade chips should be modified. In particular, by removing the parabolic-horn taper or multimode interference coupler, and injecting with an optical fiber directly, the resolving power was increased threefold from 2400 ± 200 (spectral resolution of 0.63 ± 0.2 nm) to 7000 ± 700 (0.22 ± 0.02 nm) while attaining a throughput of 77 ± 5%. More importantly, the removal of the taper enabled simultaneous off-axis injection from multiple fibers, significantly increasing the number of spectra that can be obtained at once (i.e. the observing efficiency). Here we report that ~12 fibers can be injected simultaneously within the free spectral range of our device, with a 20% reduction in resolving power for fibers placed at 0.8 mm off-centre.

Optimizing the net reflectivity of point-by-point fiber Bragg gratings: the role of scattering loss.

We present an experimental and theoretical analysis of the influence of scattering losses on the net reflectivity of fiber Bragg gratings inscribed with a femtosecond laser and the point-by-point technique. We demonstrate that the ratio of the coupling strength coefficient to the scattering loss coefficient varies significantly with the inscribing laser pulse energy, and highlight that an optimal pulse-energy range exists for achieving high-reflectivity gratings. These results are critical for exploiting high power fiber laser opportunities based on point-by-point gratings.