RESUMO
In this study, nanoparticles with both anticancer and antibacterial features were synthesized through loading chlorogenic acid (CGA) of essential oils on magnetic nanoparticles (MNPs). Characterization of γ-Fe2O3@SiO2-CGA MNPs was performed using Fourier transform infrared (FT-IR) spectroscopy and transmission electron microscopy (TEM) that show effective coating of the MNPs with SiO2 and CGA ligand and spherical shape of the nanoparticles with a mean diameter of 16 nm, respectively. The cytotoxicity study demonstrated that γ-Fe2O3@SiO2-CGA MNPs had fewer toxic effects on normal cells (Huvec) than on cancerous cells (U-87 MG, A-2780 and A-549), and could be a new potential candidate for use in biological and pharmaceutical applications. The interaction of calf thymus deoxyribonucleic acid (ct-DNA) with γ-Fe2O3@SiO2-CGA MNPs indicated that the anticancer activity might be associated with the DNA binding properties of γ-Fe2O3@SiO2-CGA MNPs. Moreover, the interaction of γ-Fe2O3@SiO2-CGA MNPs with human serum albumin (HSA) suggests that the native conformation of HSA was preserved at the level of secondary structure, indicating that the γ-Fe2O3@SiO2-CGA MNPs do not show any cytotoxicity effect when they are injected into the blood. Antibacterial tests were performed and represented γ-Fe2O3@SiO2-CGA MNPs attained better antibacterial function than CGA as free.
Assuntos
Nanopartículas de Magnetita , Nanopartículas , Humanos , Albumina Sérica Humana , Ácido Clorogênico/farmacologia , Dióxido de Silício/química , Espectroscopia de Infravermelho com Transformada de Fourier , Nanopartículas/química , Antibacterianos/farmacologia , Preparações Farmacêuticas , DNA/química , Nanopartículas de Magnetita/químicaRESUMO
In this investigation, Fe3O4 magnetic nanoparticles (MNPs) were prepared via a chemical coprecipitation reaction, and the surface of Fe3O4 MNPs was coated with silica by a sol-gel process. The surface of Fe3O4@SiO2 MNPs was modified by an antioxidant agent, trans-ferulic acid, to achieve water-soluble MNPs for biological applications. Fourier transform infrared spectroscopy (FT-IR) showed that the MNPs were successfully coated with SiO2 and ferulic acid (FA) ligand. The morphology of γ-Fe2O3@SiO2-FA MNPs was found to be spherical in images of transmission electron microscopy (TEM) and showed a uniform size distribution with an average diameter of 21 nm. The in vitro cytotoxic activity of γ-Fe2O3@SiO2-FA MNPs and FA were investigated against the human cancer cells (MCF-7, PC-3, U-87 MG, A-2780, and A-549) by MTT colorimetric assay. The cytotoxic effect of MNPs on all cancer cell lines was several times of magnitude higher compared to free FA except for A-549 cell lines. Furthermore, in vitro DNA binding studies were investigated by UV-vis and circular dichroism spectroscopies.
Assuntos
Antineoplásicos , Nanopartículas , Antineoplásicos/farmacologia , Antioxidantes/farmacologia , Ácidos Cumáricos , DNA , Humanos , Ligantes , Nanopartículas/química , Dióxido de Silício/química , Espectroscopia de Infravermelho com Transformada de Fourier , ÁguaRESUMO
Human Serum Albumin, a plasma protein existing in abundance, was selected as a template and reducing agent for the formation of CdNCs due to two factors: its stability and low cost. In the presence of human serum albumin (HSA), a selective and sensitive, low-cost, environmental friendly, and label-free off-on fluorescent sensor was synthesized and characterized for a bioaccumulating and toxic heavy metal, Hg2+ and biothiols. HSA - CdNCs can specifically recognize Hg2+ through aggregating NCs and causing fluorescence quenching. Subsequently, with increase in the concentration of biothiols, Hg2+ was eliminated from the surface of NC, while the fluorescence was restored. The calculated limits of detection (LOD) were 55â¯pM for Hg(II) and 14â¯nM for GSH, respectively. The assay was capable of detecting Hg2+ ions and GHS at different concentrations in the range of 0.008 to 8530â¯nM and 7.5-5157â¯nM, respectively. Furthermore, the appropriate molecular mechanics (MM) as well as quantum mechanical (QM) methods were performed to optimize and the theoretical investigation of the discussed HSA-profile structures and its interactions with the Cd-NCs (one atom of Cd), Hg2+ and glutathione (G).