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1.
J Natl Cancer Inst ; 78(2): 197-202, 1987 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-3100851

RESUMO

Unscheduled DNA synthesis (UDS) was studied by quantitative autoradiography in human urothelial cells of three transformation grades (TGr I-III). Cells incubated in arginine-free medium supplemented with hydroxyurea showed dose-dependent UDS after administration of agents injurious to DNA, while the scheduled synthesis of DNA was nearly totally suppressed. UDS was demonstrated after treatment with the ultimate carcinogen N-methyl-N-nitroso-N'-nitroguanidine (CAS: 70-25-7) or with the procarcinogen 4-nitroquinoline-1-oxide (4-NQO; CAS: 56-57-5). In cultures treated with benzo[a]pyrene (CAS: 50-32-8), which requires a different activation system than that for 4-NQO, UDS was less pronounced. In 9 cell lines the average rates of UDS were inversely related to TGr. Two cell lines showed a different pattern.


Assuntos
Carcinógenos/farmacologia , Transformação Celular Neoplásica , Reparo do DNA , Bexiga Urinária/citologia , 4-Nitroquinolina-1-Óxido/farmacologia , Autorradiografia , Linhagem Celular , Relação Dose-Resposta a Droga , Células Epiteliais , Humanos , Metilnitronitrosoguanidina/farmacologia , Bexiga Urinária/efeitos dos fármacos
2.
Cell Prolif ; 28(7): 359-71, 1995 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-7548438

RESUMO

The effect of sodium butyrate on cell proliferation was studied in eight human urothelial cell lines differing in transformation grade (TGr): Hu 1752 (mortal, TGr I); HCV29 (immortal and tumorigenic, TGr II); HCV29T, T24, T24A, T24B, Hu 961A and Hu 1703He (tumorigenic, TGr III). In all cell lines, except Hu 1752, addition of 4 mM sodium butyrate at 18 h after replating resulted in a significantly decreased population of adherent cells after a further 24-48 h. This might partially be explained by detachment of cells, probably mainly S phase cells, from the substrate in the lines HCV29, HCV 29T, Hu 961A and Hu 1703He. Flow cytometric DNA analysis of the adherent cell population showed that all TGr II and III urothelial cell lines were DNA aneuploid, and that butyrate perturbed the cell cycle distribution in these cell lines, mainly by a decrease of the S phase fraction. Flow cytometric bromodeoxyuridine (BrdUrd)/DNA analysis of continuously BrdUrd labelled cultures, using a 'washless' BrdUrd/DNA staining technique, showed that butyrate inhibited the G0/1-S phase transition, indicated by a delayed depletion of BrdUrd negative G0/1 cells in the cell lines HCV29, HCV29T, T24B, Hu 961A and Hu 1703He. BrdUrd/DNA analysis further showed that butyrate inhibited the G2M-G0/1 phase transition, indicated by a pronounced accumulation of BrdUrd positive G2M cells in the cell lines HCV 29T, T24B, Hu 961A and Hu 1703He. Microscopy of HCV29T and Hu 961A cells indicated that this block did not occur in mitosis. The parental cell line T24 and the cell line T24A did not show an accumulation of BrdUrd negative G0/1 cells or BrdUrd positive G2M cells like that occurring in the derived cell line T24B.


Assuntos
Anticarcinógenos/farmacologia , Butiratos/farmacologia , Ciclo Celular/efeitos dos fármacos , Transformação Celular Neoplásica/efeitos dos fármacos , Âmnio/citologia , Aneuploidia , Bromodesoxiuridina/análise , Ácido Butírico , Ciclo Celular/genética , Divisão Celular/efeitos dos fármacos , DNA/análise , Células Epiteliais , Epitélio/efeitos dos fármacos , Citometria de Fluxo , Humanos , Células Tumorais Cultivadas/citologia , Células Tumorais Cultivadas/efeitos dos fármacos , Neoplasias da Bexiga Urinária
3.
Eur J Cancer ; 26(3): 305-10, 1990 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-2141486

RESUMO

Tumourigenic (TGrIII) human urothelial cells grown in vitro have previously been demonstrated to have a markedly decreased expression of beta 2-microglobulin and HLA-A,B,C antigens as compared to non-tumourigenic (TGrII) human urothelial cell lines. Furthermore, during 'spontaneous' in vitro transformation of a non-tumourigenic (TGrII) human urothelial cell line Hu609 into a tumourigenic (TGrIII) subline Hu609T/LLH, changes in morphology and tumourigenicity have been demonstrated to be accompanied by a decreased HLA-A,B,C expression. After malignant transformation of the non-tumourigenic (TGrII) human urothelial cell line HCV29 by DNA transfection with the v-raf oncogene, four sublines could be isolated. In this study we have investigated these sublines for their expression of membrane bound HLA-A,B,C antigens and provide further evidence that an inverse relationship exists between tumourigenicity and monomorphic HLA-A,B,C expression. Treatment of the cells with recombinant human interferon alpha for 3 days increased the expression of HLA-A,B,C antigens by 50-150% indicating that at least some of the reduced HLA-A,B,C expression could be due to decreased synthesis of HLA-A,B,C antigens. All the transfected cell lines overexpress v-raf and c-myc.


Assuntos
Antígenos HLA-A/análise , Antígenos HLA-B/análise , Antígenos HLA-C/análise , Oncogenes , Transfecção , Bexiga Urinária/imunologia , Linhagem Celular , Transformação Celular Neoplásica/genética , Transformação Celular Neoplásica/imunologia , Epitélio/imunologia , Humanos
4.
Immunol Lett ; 18(2): 115-8, 1988 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-3261274

RESUMO

The production of interleukin 2 (IL-2) by phytohaemagglutinin (PHA)-stimulated peripheral blood mononuclear cells (PBMC) from 21 patients with transitional cell carcinoma of the urinary bladder (BTCC) and 16 control blood donors was measured with a solid phase enzyme immunoassay based on the dual antibody immunometric sandwich principle. PBMC from patients with invasive BTCC (grade III-IV) showed a defect in the production of IL-2. The concentration of IL-2 in the supernatants of PBMC cultures from these patients was substantially lower (0.4 +/- 0.1 U/ml) than that observed in the supernatants of PBMC cultures from patients with non-invasive BTCC, grade II (1.5 +/- 0.7 U/ml), and from tumour-free controls (1.4 +/- 0.8 U/ml). These results suggest an immune dysfunction based on quantitatively impaired IL-2 production in patients with invasive BTCC and indicate that exogenous IL-2 could be used as an immunological response modifier for the treatment of these patients.


Assuntos
Carcinoma de Células de Transição/imunologia , Interleucina-2/biossíntese , Linfócitos/imunologia , Neoplasias da Bexiga Urinária/imunologia , Idoso , Humanos , Técnicas In Vitro , Ativação Linfocitária , Linfócitos/metabolismo , Pessoa de Meia-Idade , Fito-Hemaglutininas
5.
Immunol Lett ; 14(4): 325-30, 1987 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-3495490

RESUMO

Administration of human recombinant interleukin-2 (RIL-2) into congenitally athymic (nu/nu) mice carrying subcutaneous transplants of HeLa, HU 609T and T24B human carcinoma cells partially inhibited growth of the human tumor xenografts. In vitro activation of nu/nu spleen cells with human RIL-2 resulted in generation of killer cells showing in the 51Cr cytotoxicity assay similar levels of cytolysis as RIL-2-activated spleen cells from heterozygous (nu/+) mice. The RIL-2-activated (LAK) cells were cytotoxic for a variety of mouse and human tumors, reaching the peak of their cytotoxic activity after 3 days of cultivation in the RIL-2-containing medium. The cytotoxic activity of activated nu/nu spleen cells was significantly reduced by treatment with antibody against glycolipid asialo GM1, the differentiation antigen of natural killer (NK) cells. This finding suggests that in addition to the conventional, asialo GM1- LAK cells, asialo GM1+ activated NK cells participated in the cytotoxicity displayed by the IL-2-activated nu/nu killer spleen cells.


Assuntos
Interleucina-2/uso terapêutico , Células Matadoras Naturais/imunologia , Ativação Linfocitária/efeitos dos fármacos , Neoplasias Experimentais/terapia , Animais , Citotoxicidade Imunológica/efeitos dos fármacos , Humanos , Técnicas In Vitro , Células Matadoras Naturais/efeitos dos fármacos , Camundongos , Camundongos Nus , Transplante de Neoplasias , Neoplasias Experimentais/imunologia
6.
Immunol Lett ; 19(4): 279-82, 1988 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-3266610

RESUMO

We have prepared a retroviral expression construct, pPS-IL-2, in which human IL-2 cDNA has been inserted into the polylinker region, and have used the retroviral vector to introduce the functional IL-2 gene into a fibroblast cell line, RAT-1. Peritumoral administration of IL-2-producing RAT-1 cells into congenitally athymic (nu/nu) mice carrying subcutaneous transplants of human carcinoma cells inhibited the growth of the human tumour xenografts.


Assuntos
Engenharia Genética , Interleucina-2/genética , Neoplasias Experimentais/terapia , Animais , Fibroblastos/imunologia , Fibroblastos/transplante , Vetores Genéticos , Células HeLa/imunologia , Células HeLa/patologia , Humanos , Interleucina-2/biossíntese , Células Matadoras Naturais/imunologia , Camundongos , Camundongos Nus , Transplante de Neoplasias , Neoplasias Experimentais/genética , Neoplasias Experimentais/imunologia , Retroviridae/genética , Transformação Genética
7.
Cancer Genet Cytogenet ; 4(2): 179-84, 1981 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-6277469

RESUMO

Two among ten Epstein-Barr Virus (EBV)-transformed lymphoblastoid cell lines contained a 14q+ marker in a low frequency of the cells (2 and 9%). By means of "mesome-prosome" analysis of the G-band patterns of these markers; it was established that the additional chromosome segments of these two 14q+ markers came from chromosomes #3 and #5, respectively, and not from chromosome #8 as in the 14q+ marker of Burkitt lymphoma. Chromosome #8 was not involved at all in any changes in the ten lymphoblastoid cell lines studied.


Assuntos
Linfoma de Burkitt/genética , Aberrações Cromossômicas , Cromossomos Humanos 13-15/ultraestrutura , Herpesvirus Humano 4 , Linfócitos/ultraestrutura , Adulto , Linhagem Celular , Cromossomos Humanos 1-3 , Cromossomos Humanos 4-5 , Cromossomos Humanos 6-12 e X , Marcadores Genéticos , Humanos , Cariotipagem , Linfócitos/microbiologia
8.
Anticancer Res ; 11(1): 217-23, 1991.
Artigo em Inglês | MEDLINE | ID: mdl-1708220

RESUMO

Malignant human urothelial cell lines propagated in vitro have previously been demonstrated to express low amounts of monomorphic HLA-A,B,C as compared to premalignant urothelial cells. In this study the expression of polymorphic HLA-A,B epitopes in human urothelial cell lines have been investigated in greater detail. The expression of HLA-B locus coded epitopes in malignant TGrIII cells was demonstrated to be low or absent as compared to pre-malignant TGrII or slightly transformed TGrI cells, suggesting a mechanism by which malignant cells could escape from the host immune response. The extreme polymorphism of HLA-A,B,C antigens suggests that HLA typing could be used as a method to identify the origin of cell lines which is essential in the study of the process of malignant transformation in vitro, or when correlating in vitro data with clinical observations of the patient. Two urothelial cell lines classified as slightly transformed (TGrI) and two as pre-malignant (TGrII) could, according to their expression of polymorphic HLA-A,B epitopes, be identified as genuine independent cell lines. One TGrII cell line previously designated Hu1734 shared the same HLA-A,B phenotype as the genuine HCV29 (TGrII) cell line and is therefore suspected to be a subline of the latter. Out of 18 cell lines and sublines classified as TGrIII the fidelity of four (Hu1922 and three sublines of T24) was proved by their HLA-A,B phenotype. Mistaken identity either by contamination or false labelling of the cultures was suspected in samples of six TGrIII cell lines and sublines. In four of these the HLA-A,B type characteristic for the T24 cell line could be demonstrated, but in general HLA typing of TGrIII cell lines as a method to identify the origin of the individual cell lines failed, primarily due to the decreased expression of HLA-A,B,C antigens and the apparent selective loss of HLA-B locus coded antigens.


Assuntos
Antígenos HLA-A/genética , Antígenos HLA-B/genética , Polimorfismo Genético , Divisão Celular/efeitos dos fármacos , Epitélio , Epitopos/análise , Antígenos HLA-A/análise , Antígenos HLA-B/análise , Humanos , Interferon Tipo I/farmacologia , Linfócitos/imunologia , Proteínas Recombinantes , Bexiga Urinária , Neoplasias da Bexiga Urinária
9.
Anticancer Res ; 10(2A): 359-67, 1990.
Artigo em Inglês | MEDLINE | ID: mdl-2346309

RESUMO

Eight human urothelial cell lines established and propagated in the Fibiger Institute, differing in their transformation grade as described by Christensen et al were brought into suspension and contacted with a panel of seven substrates, ie. Con. A, Fibronectin, WGA, Collagen IV, Laminin, PNA and artificial basal membrane. Cell adhesion was measured on big cell populations with the use of flow cytophotometry, measured samples contained nonadhering cells and fluorescent beads, added as an internal standard. It was found that the cell lines differed from each other by the kinetics of cell adhesion to the chosen panel. A higher percentage of cells belonging to established cell lines adhered to PNA, WGA or Con A coated surfaces than to laminin, fibronectin or collagen IV. The difference in adherence of cells to fibronectin, laminin or collagen IV coated surfaces makes differentiation between the single cell lines possible. Discriminant analysis of the total patterns of adhesion of all cell lines was used for comparison. The effect of trypsinization on the structure of the cell surface, accompanied by possible changes in adherence, was evaluated by comparison of the immediate results with those obtained with cells after 3 hours of recovery at 37 degrees C. It was found that all cell lines showed a better adherence to laminin after recovery. This is consistent with the published data on the high sensitivity of laminin receptors to trypsinization. On the other hand, the receptors for PNA, WGA and Con A were less expressed after the recovery period, as evidenced by a decreased adherence of the cells to these three substrates. The effect of recovery of cells on the amount of their receptors for fibronectin and collagen IV was different in different cell lines, but the direction of change for this pair of receptors was identical in a given cell line. In some instances the recovery period had no effect whatsoever on cell adherence. The different pattern of adhesion of cells belonging to established cell lines to the chosen panel of substrates can be used as a parameter for characterization of single cell lines, but it does not correlate with their grade of transformation.


Assuntos
Ureter/patologia , Bexiga Urinária/patologia , Adesão Celular , Linhagem Celular Transformada , Separação Celular , Epitélio , Citometria de Fluxo , Humanos
10.
Anticancer Res ; 7(3 Pt B): 481-90, 1987.
Artigo em Inglês | MEDLINE | ID: mdl-3631908

RESUMO

Human urothelial cell lines propagated in culture have been classified into three grades of transformation (TGr I-III). This classification is unrelated to the histological classification of the original biopsy material. Mortal TGr I cell lines differed from immortal and nontumorigenic TGr II cell lines and tumorigenic TGr III cell lines; they have a lower growth rate, growth fraction, and saturation density, and a higher requirement for Ca2+. Except for one TGr II cell line, TGr II cells differed from TGr III cells by having a higher serum requirement and a lower saturation density. The ability to grow in agar was not correlated with the TGr of the cell lines.


Assuntos
Transformação Celular Neoplásica , Ureter/patologia , Bexiga Urinária/patologia , Fenômenos Fisiológicos Sanguíneos , Cálcio/farmacologia , Carcinoma de Células de Transição/patologia , Contagem de Células , Linhagem Celular , Meios de Cultura , Substâncias de Crescimento/farmacologia , Humanos , Neoplasias da Bexiga Urinária/patologia
11.
Anticancer Res ; 1(4): 235-41, 1981.
Artigo em Inglês | MEDLINE | ID: mdl-7049051

RESUMO

ST/a splenocytes and peritoneal macrophages sensitized to tumorigenic, isoimmunizing R+ ST-L cells in vivo are stimulated to produce a DNA inhibitory supernatant in vitro when co-cultured with R+ ST-L cells. Studying the cross-reactions with various uninfected and MuLV infected cells, evidence was obtained for the gp70 specificity of this stimulation. T-cell depletion by thymectomy and whole body irradiation caused a moderate reduction of the DNA inhibitory effect of the splenocyte culture supernatant. However, macrophage depletion by silicate caused an almost complete abolishment of DNA inhibitory cytokine production, which could not be restored by the addition of supplementary non-sensitized spleen cells.


Assuntos
Produtos Biológicos/biossíntese , DNA de Neoplasias/antagonistas & inibidores , Neoplasias Experimentais/patologia , Animais , Transformação Celular Neoplásica/patologia , Células Cultivadas , Reações Cruzadas , Citocinas , Fibroblastos/imunologia , Imunidade Celular , Pulmão/imunologia , Depleção Linfocítica , Ativação de Macrófagos , Camundongos , Neoplasias Experimentais/imunologia , Baço/imunologia , Timectomia
12.
Anticancer Res ; 12(5): 1695-8, 1992.
Artigo em Inglês | MEDLINE | ID: mdl-1444237

RESUMO

Experiments were designed to assess age-related changes in the transplantability of human tumours xenografted in congenitally athymic (nu/nu) mice. It has been found that the number of progressively growing human tumour xenografts decreased significantly with increasing age of BALB/c nu/nu recipients. These findings, taken together with a previously recognized increase in the frequency of endogenous interleukin 2 (IL-2)-producing cells with age of nu/nu mice, prompted us to investigate whether administration of exogenous IL-2 to young adult nu/nu mice could change the transplantability of human tumours in the mice. Peritumoral administration of exogenous interleukin 2 to 8-week-old nu/nu mice inhibited the growth of the human tumour xenografts. In vitro activation of nu/nu splenocytes with exogenous Il-2 resulted in the generation of killer cells which have been found to be cytolytic when allowed to react with human tumour targets in 51Cr cytotoxicity assay. In addition, it has been found that the percentage of IL-2-activated Thy 1.2+ and ASGM1+ cells substantially increased with increasing age of nu/nu spleen cell donors. These findings are compatible with the hypothesis that the observed age-related decrease in takes of human tumour xenografts might be determined by the increasing level of IL-2 production and subsequent maturation of IL-2-dependent effector cells.


Assuntos
Envelhecimento/fisiologia , Carcinoma de Células de Transição/patologia , Linfoma de Células T/patologia , Camundongos Nus/crescimento & desenvolvimento , Transplante de Neoplasias , Sarcoma Experimental/patologia , Neoplasias da Bexiga Urinária/patologia , Neoplasias do Colo do Útero/patologia , Animais , Antígenos de Superfície/análise , Divisão Celular , Citotoxicidade Imunológica , Feminino , Citometria de Fluxo , Células HeLa , Humanos , Interleucina-2/farmacologia , Células Matadoras Ativadas por Linfocina/imunologia , Camundongos , Baço/crescimento & desenvolvimento , Baço/imunologia , Transplante Heterólogo
13.
Anticancer Res ; 13(6A): 2187-91, 1993.
Artigo em Inglês | MEDLINE | ID: mdl-7905254

RESUMO

Restriction fragment length polymorphism (RFLP) and marker chromosome or isozyme analysis were used to study the identity of 7 human urothelial cell lines selected according to their HLA-A,B phenotype. The results confirmed that the cell lines Hu961b, Hu1125, Hu1694, Hu1752, Hu609 and HCV-29 were genuine cell lines of different origin, while Hu1734 and HCV-29 were of the same origin. Hu609 was recorded to be derived from a female. However, the presence of a Y chromosome indicated either false labelling or cross-contamination. Analysis of a series of frozen stocks of Hu609 cultures showed that "malignant transformation" of these cultures was due to cross-contamination.


Assuntos
Transformação Celular Neoplásica , Bexiga Urinária/fisiologia , Linhagem Celular , Mapeamento Cromossômico , Epitélio/imunologia , Epitélio/fisiologia , Marcadores Genéticos , Globinas/genética , Antígenos HLA-A/análise , Antígenos HLA-B/análise , Humanos , Imunofenotipagem , Hibridização In Situ , Masculino , Polimorfismo de Fragmento de Restrição , Bexiga Urinária/imunologia , Cromossomo Y
14.
Anticancer Res ; 9(1): 103-7, 1989.
Artigo em Inglês | MEDLINE | ID: mdl-2650614

RESUMO

The Thomsen-Friedenreich (TF) antigen is a cryptic disaccharide structure on human erythrocytes which can be exposed by neuraminidase treatment and which is supposed to be expressed in an unmasked form on some carcinoma cells. For its detection in addition to auto-, allo- and heteroantisera, PNA (peanut lectin) is being applied. In the present studies the mouse monoclonal antibody (MoAb) raised to asialoglycophorin from human erythrocytes was used. The MoAb 22.19 is of mouse IgM isotype and is specifically binding to beta-D Gal-1-3 alpha-D GalNAc. The human urothelial cell lines maintained and characterized earlier were analyzed using indirect immunofluorescence assays. Among the spectrum of cell lines tested, five out of six cell lines belonging to the transformation grade III category (invasive in vitro and tumorigenic in nude mice) expressed TF antigen. The relationship between expression of TF antigen and other earlier defined biological traits related to malignant phenotype is discussed.


Assuntos
Anticorpos Monoclonais , Antígenos Glicosídicos Associados a Tumores , Dissacarídeos/análise , Glicoforinas/imunologia , Lectinas , Sialoglicoproteínas/imunologia , Neoplasias da Bexiga Urinária/imunologia , Imunofluorescência , Humanos , Aglutinina de Amendoim , Células Tumorais Cultivadas
15.
Anticancer Res ; 7(5B): 959-69, 1987.
Artigo em Inglês | MEDLINE | ID: mdl-3435050

RESUMO

Explantation and cultivation in vitro of 55 human biopsies of histologically normal urothelial tissue, and 63 biopsies from transitional cell carcinomas (TCC) revealed a primary success rate of about 75% and a secondary success rate of about 50% with no significant differences, between normal and TCC derived cultures. While only half of the subcultured biopsies of normal origin were able to grow for more than 4 passages all TCC derived cultures were able to do so. Based on morphology, life span, invasive growth into co-cultured fragments of embryonic chick heart, and tumorigenicity in nude mice the following transformation grades (TGr) were defined: Normal (TGr 0), non-malignant with prolonged, but finite life span (TGr I), premalignant with infinite life span but without tumorigenic and invasive potential (TGr II), malignant with infinite life span and tumorigenic and invasive properties (TGr III). Differentiation between TGr I - TGr III was possible by several other parameters, including cytological characteristics, molecular and immunological surface structure, relative oncogene expression, pyruvate kinase isoenzymes, DNA Repair, and growth pattern. However, in addition to these common characteristics, the individual cell lines of the various categories of transformation showed differences that allowed the identification of well defined cell lines and sublines.


Assuntos
Neoplasias da Bexiga Urinária/patologia , Animais , Divisão Celular , Transformação Celular Neoplásica , Feminino , Humanos , Masculino , Camundongos , Invasividade Neoplásica , Células Tumorais Cultivadas
16.
Anticancer Res ; 6(5): 1237-44, 1986.
Artigo em Inglês | MEDLINE | ID: mdl-3800330

RESUMO

The karyotypes and selected isoenzymes of five cell lines, HU 961b, HU 1922, HU 609, HCV-29 and HU 1703 He, were characterized. These cell lines were derived either from histologically normal human urothelial cells or from transitional cell carcinoma (TCC) of the urinary bladder. They displayed different life span in vitro and transplantability in nude mice, as well as a unique isoenzyme and chromosomal pattern. In addition, various chromosomes were involved in the formation of marker chromosomes in these cell lines. Since there was a lack of consistency in chromosomal changes in the cell lines with TCC origin, the cause of malignancy could not be attributed to the abnormality of specific chromosomes.


Assuntos
Cromossomos Humanos/análise , Isoenzimas/análise , Neoplasias da Bexiga Urinária/genética , Bexiga Urinária/análise , Carcinoma de Células de Transição/enzimologia , Carcinoma de Células de Transição/genética , Linhagem Celular , Humanos , Técnicas In Vitro , Cariotipagem , Invasividade Neoplásica , Bexiga Urinária/enzimologia , Neoplasias da Bexiga Urinária/enzimologia
17.
Anticancer Res ; 4(6): 319-37, 1984.
Artigo em Inglês | MEDLINE | ID: mdl-6083740

RESUMO

Human urothelial cell lines of non-malignant and malignant origin were classified into three grades of transformation, i.e. TGr I, TGr II, and TGr III, which differ from primary and early cultures of normal bladder mucosa (TGr 0) by growth in the absence of fibroblasts, prolonged survival, increasing chromosomal abnormalities, and antigenic modifications. Non-malignant pure epithelial cell lines with a prolonged, but finite life span (18-38 passager in vitro) were classified as TGr I. These cells were characterized by chromosomal counts showing a considerable variation but with a near-diploid mode and a smaller size but otherwise normal morphology. TGr II cells differed from TGr I by infinite growth transformation and wide morphological variability. TGr III cells were above all characterized by their ability to produce tumors in nude mice and to invade and destroy fragments of embryonic heart in vitro. These immortalized cells were small with a large nucleus and a uniform polygonal shape. Chromosomal counts showed pronounced aneuploidy with an increased number of chromosomes.


Assuntos
Transformação Celular Neoplásica/patologia , Neoplasias da Bexiga Urinária/patologia , Idoso , Animais , Carcinoma de Células de Transição/patologia , Linhagem Celular , Embrião de Galinha , Epitélio/patologia , Coração , Histocitoquímica , Humanos , Cariotipagem , Queratinas/análise , Neoplasias Pulmonares/secundário , Camundongos , Camundongos Nus , Microscopia Eletrônica , Microscopia de Contraste de Fase , Pessoa de Meia-Idade , Invasividade Neoplásica , Bexiga Urinária/citologia , Bexiga Urinária/ultraestrutura , Neoplasias da Bexiga Urinária/genética
18.
Neoplasma ; 37(2): 149-58, 1990.
Artigo em Inglês | MEDLINE | ID: mdl-1692978

RESUMO

We have analyzed 14 human urothelial cell lines in two different transformation grades for the production of a tumor-associated-alpha-2-macroglobulin. It has been shown in radioimmunoprecipitation and immunoblotting tests that human urothelial cells in vitro do not synthesize the wide-spectrum proteinase inhibitor alpha-2-macroglobulin. Four of the tested cell lines were additionally tested for the expression of specific mRNA which resulted in negative findings. The significance of the absence of alpha-2-macroglobulin in the human urothelial cells is discussed.


Assuntos
Neoplasias da Bexiga Urinária/metabolismo , alfa-Macroglobulinas/biossíntese , Animais , Transformação Celular Neoplásica , Humanos , Melanoma/metabolismo , Invasividade Neoplásica , Testes de Precipitina , Coelhos , Células Tumorais Cultivadas , alfa-Macroglobulinas/imunologia
19.
Folia Biol (Praha) ; 32(3): 209-11, 1986.
Artigo em Inglês | MEDLINE | ID: mdl-3488924

RESUMO

In previous communications we have demonstrated that crude rat interleukin 2 and partially purified mouse interleukin 2 were capable of inhibiting growth of transplantable, MC-induced mouse sarcomas in syngeneic recipients. Here we report that repeated peritumoral injections of highly purified human recombinant interleukin 2 can inhibit growth of these mouse sarcomas and prolong survival of tumour-bearing mice. These findings taken together and the ready availability of high doses of recombinant human interleukin 2 substantiate our proposal for initiation of clinical trials using local administration of the interleukin 2 (Bubeník et al. 1983) in selected cancer patients.


Assuntos
Interleucina-2/imunologia , Sarcoma Experimental/terapia , Animais , Humanos , Imunoterapia , Interleucina-2/isolamento & purificação , Metilcolantreno , Camundongos , Camundongos Endogâmicos , Sarcoma Experimental/induzido quimicamente
20.
Folia Biol (Praha) ; 32(3): 183-94, 1986.
Artigo em Inglês | MEDLINE | ID: mdl-3488923

RESUMO

Highly purified recombinant human interleukin 2 induced cytotoxicity of lymphocytes from urinary bladder carcinoma patients and from control healthy donors when added during an 18-h 51Cr microcytotoxicity assay against bladder carcinoma (T24) target cells. Similar levels of killer cell activation were detected in mononuclear cell preparations from bladder carcinoma patients and control healthy donors; hence, no defect in the responsiveness of bladder carcinoma patients' lymphocytes to interleukin 2 could be observed. The effect of the recombinant interleukin 2 was dose-dependent. Addition of monoclonal antibody 7E9 directed against cell-type restricted antigen associated with the T24 target cells and capable of inducing antibody-dependent cellular cytotoxicity could not increase the cytotoxicity-inducing effects of interleukin 2.


Assuntos
Interleucina-2/imunologia , Células Matadoras Naturais/imunologia , Neoplasias da Bexiga Urinária/imunologia , Anticorpos Monoclonais , Citotoxicidade Celular Dependente de Anticorpos , Citotoxicidade Imunológica , Humanos , Imunoterapia , Técnicas In Vitro , Ativação Linfocitária , Neoplasias da Bexiga Urinária/terapia
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