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1.
Methods Inf Med ; 47(6): 529-40, 2008.
Artigo em Inglês | MEDLINE | ID: mdl-19020689

RESUMO

OBJECTIVE: We created and validated a Markov model to simulate the prognosis with treatment for HCV-related hepatocellular carcinoma (HCC) for assessment of cost-effectiveness for alternative treatments of HCC. METHOD: Markov state incorporated into the model consisted of the treatment as a surrogate for HCC stage and underlying liver function. Retrospective data of 793 patients from three university hospitals were used to determine Kaplan-Meier survival curves for each treatment and transition probabilities were derived from them. RESULTS: There was substantial overlap in the 95% CIs of the Markov model predicted and the Kaplan-Meier survival curves for each therapy. The predicted survival curves were also similar with those from the nationwide survey data supporting the external validity of our model. CONCLUSIONS: Our Markov model estimates for prognosis with HCC have both internal and external validity and should be considered applicable for estimating cost-effectiveness related to HCC.


Assuntos
Carcinoma Hepatocelular/tratamento farmacológico , Hepatite C/tratamento farmacológico , Idoso , Carcinoma Hepatocelular/economia , Carcinoma Hepatocelular/etiologia , Carcinoma Hepatocelular/mortalidade , Carcinoma Hepatocelular/fisiopatologia , Intervalos de Confiança , Análise Custo-Benefício , Progressão da Doença , Feminino , Hepatite C/complicações , Hepatite C/economia , Hepatite C/mortalidade , Humanos , Masculino , Cadeias de Markov , Pessoa de Meia-Idade , Modelos Estatísticos , Probabilidade , Prognóstico , Estudos Retrospectivos , Sobrevida
2.
J Gastroenterol ; 29(1): 35-40, 1994 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-8199694

RESUMO

To investigate the specific phenotype of hyperplastic nodules (HPN) in rat liver, we produced a monoclonal antibody (HAM-6). HAM-6 was a member of the mouse IgG1 class and was specific for hyperplastic nodules, as shown by cellular radioimmunoassay and immunohistochemical studies. The antigen recognized by HAM-6 was located in the rat HPN cell membrane. HAM-6 was also slightly reactive to rat hepatocellular carcinoma, but not to normal or fetal rat liver, other normal rat organs, human hepatocellular carcinoma, or human liver cirrhosis. That is, the antigen recognized by HAM-6 appeared to be differentiated and to occur during chemical carcinogenesis. HAM-6 may be a useful marker for the investigation of premalignant states in chemical carcinogenesis.


Assuntos
Anticorpos Monoclonais/imunologia , Fígado/patologia , 2-Acetilaminofluoreno , Animais , Anticorpos Monoclonais/biossíntese , Antígenos de Neoplasias/análise , Carcinoma Hepatocelular/induzido quimicamente , Carcinoma Hepatocelular/imunologia , Dietilnitrosamina/análogos & derivados , Hiperplasia , Imuno-Histoquímica , Fígado/efeitos dos fármacos , Fígado/imunologia , Neoplasias Hepáticas/induzido quimicamente , Neoplasias Hepáticas/imunologia , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Fenótipo , Lesões Pré-Cancerosas/induzido quimicamente , Lesões Pré-Cancerosas/imunologia , Radioimunoensaio , Ratos , Ratos Endogâmicos F344
3.
J Gastroenterol ; 32(3): 330-7, 1997 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-9213246

RESUMO

The human hepatocellular carcinoma (HCC) cell line, HLF, expresses only mutant-type p53 (mt-p53), which has an amino acid substitution at the 244th residue from glycine to alanine. HLF cells were transfected with wild-type p53 (wt-p53) cDNA construct pC53-SN3, mt-p53 cDNA construct pC53-SCX [which differs by a single nucleotide, resulting in alanine instead of valine at the 143rd residue in p53 (p53-143)], or pCMV-Neo-Bam, as a control, by a liposome method. After G418 selection, three wt-p53 stable transformants (WT), four mt-p53 transformants (MT), and three control vector transformants (VT) were obtained. We analyzed the cell growth and morphological changes of these transformants under different culture conditions [fetal calf serum (FCS), 10%, 1%, and 0%]. Whereas no difference from control in the growth rate and morphology was observed under the 10% FCS conditions, serum starvation induced remarkable phenotypical changes in all three WTs, but not in the other transformant. Corresponding to these phenotypical changes, the transcriptional activity of wt-p53 was increased more than nine fold. These results indicated that serum starvation would induce wt-p53 biological function, which is tightly linked to morphological changes and growth suppression. To induce these changes, the introduction of the wt-p53 gene itself was not sufficient, and additional triggering, i.e., serum starvation, was indispensable.


Assuntos
Carcinoma Hepatocelular/patologia , Genes p53/fisiologia , Neoplasias Hepáticas/patologia , Carcinoma Hepatocelular/genética , Humanos , Neoplasias Hepáticas/genética , Fenótipo , Transcrição Gênica , Transfecção , Células Tumorais Cultivadas , Proteína Supressora de Tumor p53/metabolismo
4.
Hepatogastroenterology ; 43(10): 926-31, 1996.
Artigo em Inglês | MEDLINE | ID: mdl-8884315

RESUMO

BACKGROUND/AIMS: This study attempts to clarify the clinicopathologic definition of early hepatocellular carcinomas (HCCs). MATERIALS AND METHODS: We evaluated 57 patients, with HCCs less than 3 cm in diameter, in terms of prognosis, incidence of extrahepatic metastasis, and tumor recurrence rate following treatment. RESULTS: Survival was related to both tumor number and histologic differentiation, but was not related to tumor size. Furthermore, prognosis appeared to depend on the functional reserve of the liver. The incidence of extrahepatic metastasis was related to histologic differentiation. There was no significant difference in the recurrence rates of patients with uninodular tumors in terms of tumor size. CONCLUSIONS: Our findings indicate that early HCCs measure 15 mm or less in diameter, are uninodular, and are histologically well-differentiated. Finally, the functional reserve of the liver will likely be an additional parameter that will further characterize early HCCs.


Assuntos
Carcinoma Hepatocelular/patologia , Neoplasias Hepáticas/patologia , Fígado/patologia , Carcinoma Hepatocelular/diagnóstico , Carcinoma Hepatocelular/mortalidade , Carcinoma Hepatocelular/secundário , Feminino , Humanos , Neoplasias Hepáticas/diagnóstico , Neoplasias Hepáticas/mortalidade , Masculino , Pessoa de Meia-Idade , Recidiva Local de Neoplasia/epidemiologia , Prognóstico , Análise de Sobrevida , Taxa de Sobrevida
5.
Rinsho Byori ; 39(6): 597-603, 1991 Jun.
Artigo em Japonês | MEDLINE | ID: mdl-1715410

RESUMO

By dilution of the serum, we quantitatively analyzed anti-HCV to examine the relation between the anti-HCV titer and the state of chronic hepatitis C, and determined the effectiveness of interferon therapy. The anti-HCV titer was related with the state of chronic hepatitis C, but not as closely as GPT. Cases with blood transfusion had a lower titer of anti-HCV, weaker histological finding and better response to interferon than those without blood transfusion. The anti-HCV titer was not useful for determining the effectiveness of interferon therapy.


Assuntos
Hepatite C/patologia , Adulto , Fatores Etários , Feminino , Anticorpos Anti-Hepatite/análise , Hepatite C/diagnóstico , Humanos , Interferons/uso terapêutico , Masculino , Pessoa de Meia-Idade , Fatores Sexuais , Reação Transfusional
6.
Gan To Kagaku Ryoho ; 19(5): 612-7, 1992 May.
Artigo em Japonês | MEDLINE | ID: mdl-1580635

RESUMO

We investigated the relationship between the growth of HCC and nutrition, especially amino acids, and reconsidered the clinical application of amino acid imbalance. At first, rat chemical hepato-carcinogenesis was performed to investigate whether Aminoleban EN stimulates or restrains the occurrence of HCC. 2-Acetyl-amino-fluorene containing diet was administered intermittently according to Epstein's method. Rats were divided into two groups; group 1 was fed on Aminoleban EN containing diet and group 2 on a basal diet. There was no significant difference between the survival rate in the two groups. The average body weight of group 1 was significantly higher than that of group 2. The rats were sacrificed at the 25th week. All 11 rats of group 1 had no liver tumor, but 2 of 17 rats of group 2 had liver tumors, including a HCC and cholangiocellular carcinoma. The incidence of the liver tumor was significantly different between the two groups. Aminoleban EN could inhibit rat liver carcinogenesis, so it is considered to be a desirable nutritional product for LC patients from the stand point of cancer prevention. Secondly, the composition of amino acid was studied on HCC and surrounding tissue. There was no significant difference of Val, Leu, Leu, Phe, Tyr, Met and Fischer ratio between HCC and surrounding tissue.


Assuntos
Aminoácidos/administração & dosagem , Alimentos Formulados , Neoplasias Hepáticas Experimentais/metabolismo , Aminoácidos/metabolismo , Animais , Neoplasias Hepáticas Experimentais/induzido quimicamente , Neoplasias Hepáticas Experimentais/patologia , Masculino , Ratos
7.
Gan To Kagaku Ryoho ; 22(11): 1669-72, 1995 Sep.
Artigo em Japonês | MEDLINE | ID: mdl-7574789

RESUMO

We report a case of multiple liver metastasis from ileac carcinoid treated with continuous intraarterial infusion of somatostatin analog. A 65-year-old man who complained of chest pain was admitted to Yamaguchi University Hospital School of Medicine for further examination of cardiac angina. Liver tumors, which were detected during ECHO cardiogram examination, were diagnosed as metastasis from carcinoid by percutaneous transhepatic liver biopsy. Primary tumor was found at the ileum by colonofiberscopy. We performed ileo-cecal resection and catheterization from the gastroduodenal artery for intraarterial chemotherapy under laparotomy. After the operation, the patient was treated with continuous intraarterial infusion of somatostatin analog (100 micrograms/day, 5 days/week for 16 weeks). The tumor in segment 6 (S6) disappeared, but the tumor in S2 enlarged after the therapy. Hepatic angiography confirming the drug distribution demonstrated the occlusion of the left hepatic artery. This drug was thus distributed to the tumor in S6 but not in S2. These results suggest that somatostatin analog may have a direct anti-tumor effect. Furthermore, no side effect was observed. Thus, intraarterial infusion of somatostatin analog may be a useful therapy for liver metastasis from carcinoid.


Assuntos
Antineoplásicos Hormonais/administração & dosagem , Tumor Carcinoide/tratamento farmacológico , Tumor Carcinoide/secundário , Neoplasias do Íleo/patologia , Neoplasias Hepáticas/tratamento farmacológico , Neoplasias Hepáticas/secundário , Octreotida/administração & dosagem , Idoso , Tumor Carcinoide/cirurgia , Terapia Combinada , Esquema de Medicação , Artéria Hepática , Humanos , Bombas de Infusão Implantáveis , Infusões Intra-Arteriais , Neoplasias Hepáticas/cirurgia , Masculino
12.
Tumour Biol ; 9(4): 170-7, 1988.
Artigo em Inglês | MEDLINE | ID: mdl-3420373

RESUMO

Two monoclonal antibodies (MoAbs) were prepared against the hepatocytes of hyperplastic liver nodules from rats killed in the 13th week during hepatocarcinogenesis with 2-acetylaminofluorene. The specificity of these MoAbs was confirmed by cellular radioimmunoassay. By means of immunoperoxidase staining with 2 MoAbs (HAM 6 and HAM 7), HAM 6-defined antigen was detected on the plasma membrane and/or cytoplasm of the hepatocytes in hyperplastic nodules, while HAM 7 reacted to the hepatocytes surrounding hyperplastic nodules. Therefore, HAM 6 seemed to be specific to hyperplastic nodules, but HAM 7 to be a MoAb developed accidentally to the surrounding hepatocytes by contamination of the cell suspension of hyperplastic nodules.


Assuntos
Anticorpos Monoclonais , Antígenos de Neoplasias/análise , Neoplasias Hepáticas Experimentais/imunologia , Lesões Pré-Cancerosas/imunologia , 2-Acetilaminofluoreno , Animais , Hibridomas/imunologia , Imunoglobulina G/análise , Neoplasias Hepáticas Experimentais/induzido quimicamente , Masculino , Ratos , Ratos Endogâmicos F344
13.
Cancer ; 91(7): 1342-8, 2001 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-11283935

RESUMO

BACKGROUND: Radiofrequency ablation (RFA) for patients with hepatocellular carcinoma (HCC) has been reported previously. This technique is superior to percutaneous microwave coagulation therapy (PMCT) for the enlargement of the necrotic area. Therefore, a few treatment sessions of RFA for patients with small HCC lesions measuring < 3 cm in greatest dimension can achieve complete necrosis. To achieve this with a one-treatment RFA session, the authors designed the technique of RFA with angiography combined with computed tomography (angio-CT) assistance. The advantages of this technique are that it is possible to detect small satellite nodules and to evaluate the real-time therapeutic effect immediately after RFA. METHODS: Ten patients with 12 HCC lesions measuring < 4 cm in greatest dimension underwent RFA with angio-CT assistance. The authors performed standard RFA for six patients (seven tumors) and RFA with balloon occlusion of the hepatic artery (balloon-occluded RFA [BoRFA]) for four patients (five tumors). Final therapeutic efficacy was evaluated with dynamic CT scans performed 2 weeks after treatment. RESULTS: On CT arteriography (CTA) obtained immediately after treatment, a hyperattenuating ring around the nonenhanced region was apparent in all patients. On CT scans obtained 2 weeks after treatment, this ring disappeared, and the greatest dimension of the nonenhanced region was slightly larger than that on the CTA obtained immediately after treatment. The authors achieved complete eradication with one treatment session of RFA in 8 of 10 patients (80%). Local recurrence occurred in one patient 10 months after treatment. The greatest dimension of the area coagulated by BoRFA was significantly larger (greatest long-axis dimension, 38.2 +/- 2.8 mm; greatest short-axis dimension, 35.0 +/- 1.7 mm; n = 5 lesions) than without it (greatest long-axis dimension, 30.0 +/- 4.1 mm; greatest short-axis dimension, 27.0 +/- 4.3 mm; n = 4 lesions; greatest long-axis dimension, P = 0.009; greatest short-axis dimension, P = 0.006). No major complications occurred in any patient. CONCLUSIONS: The authors were able to achieve success with a single treatment session in patients with small HCC using RFA with angio-CT assistance. They consider that RFA with angio-CT assistance is a safe and effective technique for the treatment of patients with small HCC.


Assuntos
Angiografia , Carcinoma Hepatocelular/diagnóstico por imagem , Carcinoma Hepatocelular/cirurgia , Ablação por Cateter , Neoplasias Hepáticas/diagnóstico por imagem , Neoplasias Hepáticas/cirurgia , Tomografia Computadorizada por Raios X , Adulto , Idoso , Idoso de 80 Anos ou mais , Oclusão com Balão , Carcinoma Hepatocelular/irrigação sanguínea , Ablação por Cateter/métodos , Feminino , Humanos , Neoplasias Hepáticas/irrigação sanguínea , Masculino , Pessoa de Meia-Idade , Neoplasia Residual , Radiografia Abdominal
14.
J Comput Assist Tomogr ; 17(3): 390-6, 1993.
Artigo em Inglês | MEDLINE | ID: mdl-8388004

RESUMO

Breath-hold multislice dynamic MRI was used to diagnose 115 hepatic tumors in 55 patients. With this technique 10 slice-section images were made during a single breath-hold, achieving nearly complete liver coverage. Early enhancement images were obtained 20 s after intravenous bolus injection of gadopentetate dimeglumine. The detection rate of untreated hepatocellular carcinomas was greater with breath-hold multislice dynamic imaging (91%) than with conventional spin echo techniques (73%), because the 10 early enhancement images obtained during a single breath-hold showed the arterial dominant phase throughout the entire liver. In cases of untreated hepatocellular carcinoma 44 (86%) of the 51 lesions showed rapid enhancement on arterial dominant phase images, and 44 (86%) were demonstrated as isointensity or low intensity areas on delayed images. In cases of hepatic hemangioma, peripheral enhancement was demonstrated in 25 (89%) of the 28 lesions on arterial dominant phase images, and partial or complete hyperintense fill-in was identified in all lesions on delayed images. In patients treated with lipiodol-transcatheter arterial embolization (TAE), the breath-hold multislice dynamic imaging technique allowed evaluation of therapeutic effectiveness by revealing the hemodynamics of each nodule without the influence of lipiodol. We conclude that breath-hold multislice dynamic MRI should be used on patients with suspected hepatic tumors and in follow-up studies after lipiodol-TAE.


Assuntos
Carcinoma Hepatocelular/diagnóstico , Hemangioma/diagnóstico , Neoplasias Hepáticas/diagnóstico , Imageamento por Ressonância Magnética , Adulto , Idoso , Carcinoma Hepatocelular/terapia , Meios de Contraste , Embolização Terapêutica , Feminino , Gadolínio DTPA , Hemangioma/terapia , Humanos , Neoplasias Hepáticas/terapia , Imageamento por Ressonância Magnética/métodos , Masculino , Pessoa de Meia-Idade , Compostos Organometálicos , Ácido Pentético
15.
Dig Dis Sci ; 43(1): 1-7, 1998 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-9508509

RESUMO

Conventional cytogenetic studies have demonstrated frequent abnormalities of specific chromosomes in hepatocellular carcinoma, although there are few reports examining the relationship between chromosomal aberrations and clinicopathologic features. In this study, numerical aberrations of chromosomes 16, 17, and 18 were examined by fluorescence in situ hybridization using pericentromeric DNA probes in 20 cases of surgically removed hepatocellular carcinoma. DNA ploidy analysis was also performed by flow cytometry. Numerical abnormalities of chromosomes 16, 17, and 18 were found in 7 of 19 cases, 15 of 20 cases, and 12 of 20 cases, respectively. Gain and/or loss of more than one chromosome was detected in 16 of 19 cases. However, aneuploidy was seen in only 9 of 20 tumors by flow cytometry. The incidence of aneusomy 17 and 18 increased with tumor size and stage progression. Fluorescence in situ hybridization analysis demonstrated that numerical chromosomal aberrations accumulated with tumor progression in hepatocellular carcinoma.


Assuntos
Carcinoma Hepatocelular/genética , Cromossomos Humanos Par 16 , Cromossomos Humanos Par 17 , Cromossomos Humanos Par 18 , Neoplasias Hepáticas/genética , Adulto , Idoso , Aneuploidia , Diploide , Feminino , Humanos , Hibridização in Situ Fluorescente , Masculino , Pessoa de Meia-Idade , Ploidias
16.
AJR Am J Roentgenol ; 161(2): 329-34, 1993 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-8333371

RESUMO

The portacaval space is a relatively narrow region between the portal vein and the inferior vena cava that is usually well seen on cross-sectional abdominal imaging studies [1]. Various structures, including normal anatomic variants and extension of diseases from adjacent organs, can be detected within this space [1-3]. An awareness of the types of normal and abnormal structures can prevent misinterpretation and aid in evaluating the spread of malignant diseases. We describe the appearances of these entities within the portacaval space on several types of images.


Assuntos
Linfonodos/diagnóstico por imagem , Veia Porta/diagnóstico por imagem , Veia Cava Inferior/diagnóstico por imagem , Angiografia , Sistema Biliar/diagnóstico por imagem , Diagnóstico por Imagem , Humanos , Fígado/diagnóstico por imagem , Metástase Linfática/diagnóstico , Imageamento por Ressonância Magnética , Neoplasias/diagnóstico , Pâncreas/diagnóstico por imagem , Intensificação de Imagem Radiográfica , Valores de Referência , Tomografia Computadorizada por Raios X
17.
J Osaka Univ Dent Sch ; 30: 86-96, 1990 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-2130177

RESUMO

The long-term complications of dental implants are associated with loosening of the implant-bone interface, due to bone resorption and subsequent connective tissue interposing at the implant-bone interface. Mechanical stresses, which are induced in the surrounding bone, play a key role in the interface bone remodeling. In the present study, it is investigated how local stress patterns are associated with local bone structure. For identical geometry alumina, and titanium implants, the stress patterns, determined in the finite element models with boundary conditions, are compared with the local bone structure found in animal experiments. The results indicate that there appears to be relationship between the stress patterns and the bone remodeling at the implant-bone interface independently on the materials properties in alumina and titanium.


Assuntos
Remodelação Óssea , Implantes Dentários , Análise do Estresse Dentário , Óxido de Alumínio , Animais , Reabsorção Óssea , Haplorrinos , Masculino , Titânio
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