Smart textiles: a new drug delivery system for symptomatic treatment of a common cold.

Smart textiles provide the possibility of being coated with cineole, menthol, and camphor. Due to over-the-counter availability, ethereal oils are frequently used to treat a common cold. The existing pharmaceutical forms entail the risk of oral ingestion by children, which can cause severe intoxications. This risk could be limited by a smart textile application. Prior to applicability tests in children, the principal traceability of smart textile-applied ethereal oils at their site of action in the alveoli has to be demonstrated. Therefore, a crossover trial (ointment vs smart textiles) with 6 healthy volunteers was carried out as a proof-of-concept study. As a result, the principle proof is given that smart textile-applied ethereal oils are available at their site of action. Because of the volatility of the active ingredients, a close-fitting textile form has to be developed for further clinical development of smart textiles to achieve higher concentrations in the alveoli. Slower liberation properties and a more convenient skin sensation in comparison to available pharmaceutical forms may provide advantages for the applicability in both children and adults.

Genotoxicity assessment of waste products of aluminum plasma etching with the SOS chromotest.

We evaluated 36 characteristic waste products from the plasma etching of aluminum for genotoxicity with the SOS chromotest. The majority of the samples showed genotoxic activity in tester strain Escherichia coli PQ37 without metabolic activation using S9 mix. In the presence of S9, a deactivation of the samples was regularly observed. Comparable studies with the Salmonella/microsome (Ames) test using tester strains Salmonella typhimurium TA98 and TA100 indicated actual mutagenicity of waste products. Gas chromatograms of the organic constituents of all waste products were performed in parallel with the genotoxicity assays. In contrast to the similarity of the peak patterns of all chromatograms, the biological effects of individual waste samples showed large differences. Information on chemical composition and the SOS chromotest results of a representative sample recovered over a period of 2 years is given. For this sample, the influences of sample preparation and cytotoxic matrix effects on the test parameters are also shown.

Beta 2-adrenoceptor density on membranes and on intact mononuclear cells in essential hypertension.

Alterations in the status or in the regulation of adrenoceptors may contribute to essential hypertension. This could be studied using the recently introduced radio-ligand binding techniques to characterize the adrenoceptors on human peripheral blood cells. The present study shows that patients with essential hypertension have a twofold increase of beta 2-adrenoceptor density on intact mononuclear cells as compared to normotensive controls: 859 +/- 260 (n = 10) vs. 420 +/- 119 (n = 10) maximal binding sites for (+/-) 125-Iodocyanopindolol expressed as molecules per cell (P less than 0.001). Furthermore, there is a highly significant correlation (r = 0.86) between the calculated mean arterial blood pressure and the beta 2-adrenoceptor density over a wide range of normal and increased blood pressure. These findings could only be demonstrated with intact mononuclear cells but not with membrane fractions. No difference was found in receptor affinity between patients with essential hypertension and normotensive controls. Thus, essential hypertension is combined with a higher beta 2-adrenoceptor density on intact mononuclear cells which might represent, for example, an increased density of prejunctional beta 2-adrenoceptors. Mean arterial blood pressure is positively correlated with beta 2-adrenoceptor density over a wide range of blood pressure in normotensives and hypertensives. The expression of beta 2-binding sites on the cell surface is possibly altered in essential hypertension resulting in a disparity between intracellular and extracellular binding sites as compared with normotensives.