RESUMO
OBJECTIVE: To explore the possible pain mechanism of chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS). METHODS: The models of CP/CPPS were established in male Wistar rats by the autoimmune method. The paw withdrawal threshold (PWT) was detected using Von Frey filament. The expressions of the substance P and c-fos in the prostate and spinal L5-S2 segments were determined by immunohistochemistry followed by analysis of their correlation with CP/CPPS. RESULTS: Compared with the control rats, the CP/CPPS models showed significantly decreased PWT (P < 0.05), remarkable prostatic inflammation, enlarged scope of lesions, and obvious interstitial lymphocytic infiltration (P < 0.05). Both the expressions of substance P and c-fos were markedly elevated in the prostate and spinal dorsal horn (L5-S2) of the rat models (P < 0.05), but the expression of substance P in the prostate exhibited no correlation with that in the spinal cord (r = 0.099, P = 0.338), nor did that of c-fos (r = 0.027, P = 0.454). CONCLUSION: The upregulated expressions of substance P and c-fos in the spinal cord L5-S2 sections may be associated with the pain mechanism of CP/CPPS.
Assuntos
Dor Pélvica/etiologia , Próstata/metabolismo , Prostatite/complicações , Proteínas Proto-Oncogênicas c-fos/metabolismo , Medula Espinal/metabolismo , Substância P/metabolismo , Animais , Doença Crônica , Imuno-Histoquímica , Masculino , Dor Pélvica/metabolismo , Prostatite/metabolismo , Ratos , Ratos Wistar , Síndrome , Regulação para CimaRESUMO
OBJECTIVE: To study the possible mechanisms of chronic nonbacterial prostatitis (CNP) pain. METHODS: CNP models were established in male Wistar rats by the autoimmune method. Then the paw withdrawal threshold (PWT) was detected using the Von Frey filament, prostate pathological examination was conducted, the expressions of substance P (SP) and transient receptor potential vanilloid 1 (TRPV1) in the prostate tissue and L5-S2 spinal segments were determined by immunohistochemistry and their correlations were analyzed. RESULTS: Compared with the control group, the CNP model rats showed markedly decreased PWT (P < 0.05) and obvious inflammation in the prostate tissue, with significant differences in the scope of lesion and interstitial lymphocyte infiltration (P < 0.05). The expressions of SP and TRPV1 in the prostate and spinal cord dorsal horn L5-S2 were remarkably upregulated in the models as compared with the control rats (P < 0.05). However, the expression of SP in the prostate was not correlated with that in the spinal cord (r = 0.099, P = 0.338), nor was that of TRPV1 (r = 0.000, P = 0.5). CONCLUSION: SP and TRPV1 were involved in the formation and persistence of pain in CNP rats through their upregulated expressions in the L5-S2 spinal segments.
Assuntos
Neuralgia/metabolismo , Dor/metabolismo , Prostatite/metabolismo , Medula Espinal/metabolismo , Substância P/metabolismo , Canais de Cátion TRPV/metabolismo , Animais , Região Lombossacral , Masculino , Neuralgia/fisiopatologia , Dor/fisiopatologia , Próstata/metabolismo , Prostatite/fisiopatologia , Ratos , Ratos WistarRESUMO
We herein reported the first chemoenzymatic synthesis of lacto-N-hexaose (LNH) by combining chemical carbohydrate synthesis with a selectively enzymatic glycosylation strategy. A tetrasaccharide core structure GlcNH2ß1â3 (GlcNAcß1â6) Galß1â4Glc, a key precursor for subsequent enzymatic glycan extension toward asymmetrically branched human milk oligosaccharides, was synthesized in this work. When the order of galactosyltransferase-catalyzed reactions was appropriately arranged, the ß1,4-galactosyl and ß1,3-galactosyl moieties could be sequentially assembled on the C6-arm and C3-arm of the tetrasaccharide, respectively, to achieve an efficient LNH synthesis. Lacto-N-neotetraose (LNnH), another common human milk oligosaccharide, was also synthesized en route to the target LNH.
RESUMO
There are two independent 3,5-dimethyl-pyrazole and two independent 2-hy-droxy-5-(phenyl-diazen-yl)benzoic acid mol-ecules [in which intra-molecular O-Hâ¯O bonds form S(6) graph-set motifs] in the asymmetric unit of the title compound, C(5)H(8)N(2)·C(13)H(10)N(2)O(3). In the crystal, the components are linked by inter-molecular O-Hâ¯O, O-Hâ¯N and N-Hâ¯O hydrogen bonds, forming four-component clusters. Further stabilization is provided by weak C-Hâ¯π inter-actions.
RESUMO
OBJECTIVE: To establish an animal model of chronic nonbacterial prostatitis (CNP) using different doses of purified prostate protein with Freund's complete adjuvant (FCA), and to investigate the relationship of the doses with the success of the model construction. METHODS: Thirty male Wistar rats were divided into a control (A) and 4 experimental groups (B, C, D and E) of equal number. The latter 4 groups were given multi-loci intracutaneous injection of 1.0 ml of a 1:1 mixture of purified prostate protein at 20, 40, 60 and 80 mg/ml with Freund's complete adjuvant (FCA), and meanwhile intraperitoneally injected with 0.5 ml of pertussis-diphtheria-tetanus vaccine at 0 and 30 days. On the 45th day, the rats were sacrificed for observation of the pathomorphological changes in the prostate glands with the naked eyes and microscope. RESULTS: Different degrees of chronic inflammation were observed with different degrees of lymphocyte infiltration and interstitial hyperplasia in the experimental rats. More obvious changes were found in Groups C and D than in A, and even more significant in Group E (P < 0.05). CONCLUSION: The rat model of CNP can be successfully established by multi-loci intracutaneous injection of 1.0 ml of a 1: 1 mixture of purified prostate protein at 40 - 60 mg/ml with FCA, and simultaneously intraperitoneal injection of 0.5 ml of pertussis-diphtheria-tetanus vaccine twice within 30 days.