Mass Equilibration and Fluctuations in the Angular Momentum Dependent Dynamics of Heavy Element Synthesis Reactions.

Mass and angle distributions for the ^{52}Cr+^{198}Pt and ^{54}Cr+^{196}Pt reactions (both forming ^{250}No) were measured and subtracted, giving new information on fast quasifission mass evolution, and the first direct determination of the dependence of sticking times on angular momentum. TDHF calculations showed good agreement with average experimental values, but experimental mass distributions unexpectedly extended to symmetric splits while the peak yield remained close to the initial masses. This implies a strong role of fluctuations in mass division early in the collision, giving insights into the transition from fast energy dissipative deep-inelastic collisions to quasifission.

[Investigation of structural characteristics of initial substances of ibuprofen and micronization impact].

Structural characteristics of ibuprofen substances manufactured by different firms and the impact of micronization on them were compared. The study showed that the use of X-ray diffraction methods was necessary for certification of medicinals (crystalline) since only such methods provided information on the substance crystal structure, the molecular state and polymorphous forms.

Predominant time scales in fission processes in reactions of S, Ti and Ni with W: zeptosecond versus attosecond.

The inhibition of fusion by quasifission is crucial in limiting the formation of superheavy elements in collisions of heavy nuclei. Time scales of ∼10(-18) s inferred for fissionlike events from recent crystal blocking measurements were interpreted to show either that quasifission itself is slower than previously believed, or that the fraction of slow fusion-fission is higher than expected. New measurements of mass-angle distributions for (48)Ti and (64)Ni bombarding W targets show that in these reactions quasifission is the dominant process, typically occurring before the system formed after contact has made a single rotation, corresponding to time scales of ≤10(-20) s.

A New Bacillus licheniformis Mutant Strain Producing Serine Protease Efficient for Hvdrolvqis of Sov Meal Proteins.

Induced mutagenesis with y-irradiation of the industrial strain Bacillus licheniformis-60 VKM B-2366,D was used to obtain a new highly active producer of an extracellular serine protease, Bacillus licheni- formis7 145. Samples of dry.concentrated preparations of serine protease produced by the original and mutant strains were obtained, and identity of their protein composition was'established. Alkaline serine protease sub- tilisin DY was the main component of the preparations. The biochemical and physicochemical properties of the Protolkheterm-145 enzyme preparation obtained from the mutant strain were studied. It exhibited pro- teolytic activity (1.5 times higher than the preparation from the initial strain) within broad ranges of pH (5- 11) and temperature (30-70'C).-Efficient hydrolysis of extruded soy meal protein at high concentrations (2 to 50%) in-the reaction mixture was.the main advantage of the Protolikheterm 145 preparation. Compared to,. the preparation obtained using the initial strain, the new preparation with increased proteolytic-activity pro- vided for more complete hydrolysis of the main non-nutritious soy,proteins.(glycinin and 0-conglycinin) with the yield of soluble protein increased by 19-28%, which decreased the cost of bioconversion of the protein- aceous material and indicated promise of the new preparation in resource-saving technologies for processing soy meals and cakes.

Interaction of sulfhydryl reagents with K+ transport in adrenal chromaffin granules.

In the present study the functional role of SH groups in the Ca(2+)-independent K(+)-selective channel activity in the membrane of bovine adrenal gland chromaffin granules has been studied. Ionic channel activity has been estimated using 86Rb+, a K+ analogue, flux measurements. The inhibition of chromaffin granules K+ channel by SH modifying agents, such as N-ethylmaleimide, mersalyl and phenylarsenoxide, is described.

ATP-sensitive K+ transport in adrenal chromaffin granules.

In the present study the influx of 86Rb+, a K+ analogue, was studied in mitochondria, microsomes and chromaffin granules prepared from adrenal gland medulla. The most active electrogenic 86Rb+ transport was found in the membrane fraction identified as chromaffin granules by marker enzyme estimation. The transport was found to be sensitive to ATP, ATP gamma S, ADP and to the triazine dyes, but not to AMP and cAMP. The inhibition induced by ATP was observed in the absence of externally added Mg2+, suggesting that a free nucleotide, rather than the ATP-Mg complex, was required for inhibition. Furthermore, the 86Rb+ influx was found to be inhibited by Mg2+ alone, but not by Ca2+ and antidiabetic sulfonylureas. The 86Rb+ influx was not stimulated by potassium channel openers. In conclusion, our results indicate that an electrogenic, ATP-sensitive potassium transport system operates in the chromaffin granule membrane.

[Dynamics of the echocardiographic indicators in myocardial infarct]. / Dinamika ekhokardiograficheskikh pokazatelei pri infarkte miokarda.

Echocardiography was conducted on 22 patients with macrofocal myocardial infarction in the first 24 hours of the disease and in the three months following. Zones of asynergy of myocardial contraction were found in all patients. Segmental diminution of myocardial contraction was combined with hyperkinesia of areas of the intact heart muscle. Disturbance in regional myocardial contraction was attended with a decrease in functional capacity of the left ventricle. The left ventricular dysfunction in myocardial infarction is to a greater extent determined by the size and character of the asynergy rather than by the localization of infarction. In the period of recovery normalization of contraction was noted in the hypokinetic zones and to a lesser extent in the zones of akinesia. Zones of paradoxical movements of the wall which had developed in the acute period remained 3 months after the onset of the disease.

[Value of serial enzyme and isoenzyme tests in early diagnosis of myocardial infarct]. / Znachenie seriinogo opredeleniia enzimov i izoenzimov dlia rannei diagnostiki infarkta miokarda.

Serial tests for the activity of creatine phosphokinase (CPK), specific cardiac isoenzyme MB CPK, lactic dehydrogenase (LDH) and its fractions LDH1 and LDH2 were conducted in 55 patients with myocardial infarction. The control group consisted of 15 healthy persons. It was found that an increase in total CPK activity allows the diagnosis of myocardial infarction to be confirmed in the first hours after the onset of the disease, while simultaneous increase in the activity of MB CPK makes the diagnosis authentic, which is particularly valuable in cases with an atypical course of the disease. A recurrent course of myocardial infarction is attended with parallel changes in the activity of total CPK and its MV fraction. Increased total CPK activity may be due both to myocardial infarction and to extracardiac causes.

[Importance of pyrophosphate-99mTc scintigraphy in the diagnosis of acute myocardial infarct]. / Znachenie stsintigrafii s pirofosfatom-99mTc v diagnostike ostrogo infarkta miokarda.

Scintigraphy with pyrophosphate 99mTc was performed in 230 patients with various forms of ischemic heart disease and in 15 persons with pain in the region of the heart caused by osteochondrosis of the cervical and thoracic spinal segments or vegetovascular dystonia (control group). It was found that labelled purophosphate accumulated in the myocardium in necrosis of the heart muscle or when coronary insufficiency takes a course in which necrosis of the myocardial cells is quite possible. Positive results of scintigraphy with pyrophosphate 99mTc are not a strict criterion of acute myocardial infarction because they are also encountered in a chronic course of ischemic heart disease and are evidence in this case that "a state of risk" has occurred during the disease.

[Lethal and neutral temperature of a helium-oxygen atmosphere for rabbits in the presence of elevated atmospheric pressure]. / O letal'noi i neitral'noi temperaturakh gelio-kislorodnoi sredy dlia krolikov pri povyshennom atmosfernom davlenii.

The 5 to 20-day stay of the rabbits in helio-oxygen medium at the pressure of 3534 kPa revealed a linear interrelationship between the temperature 297-302 K and oxygen consumption. At the low lethal temperature of 294 K rabbits perished within 24 hrs due to growing hypothermia. At the neutral temperature 302 K the respiratory heat loss constitutes 30-38% of the basal metabolism, heat loss from the skin surface decreases. The hyperbaric medium seems to have no thermoneutral zone adequate to air by its physiological effect.

[Effect of amiloride and strophanthin K on sodium transport through isolated frog skin under increased pressure]. / Vliianie amilorida i strofantina K na transport natriia cherez izolirovannuiu kozhu liagushki pri uvelichenii davleniia.

A pressure increase up to 101 ata led to 17.8% and 50% increase of the electrical potential difference and short-circuited current, resp., as well as to a 22% decrease of electrical resistance in the isolated frog skin. The changes were due to a high hydrostatic pressure and the increase of the solution temperature by 2.8 +/- 0.14 degrees C. At 100 ata, the ability of amiloride to block Na-channels in the cell membranes of the frog skin was preserved, and inspite of the intense increase in the work of the sodium active transport system, the kinetics of inhibition of the Na-pump by strophanthin K remained the same as in case of normal pressure.

[The clinical evaluation of a change in lipid peroxidation in patients with ischemic heart disease and cardiac arrhythmias]. / Klinicheskaia otsenka izmeneniia perekisnogo okisleniia lipidov u bol'nykh ishemicheskoi bolezn'iu serdtsa s narusheniiami serdechnogo ritma.

Overall 93 patients suffering from coronary heart disease /CHD/ (without acute myocardial infarction) complicated by cardiac rhythm impairment were followed up. Investigation of lipid peroxidation (LPO) and of the antioxidant system (AOS) in those patients revealed LPO activation and a decrease of the AOS activity both at the moment of arrhythmia and after its arrest, with the highest LPO activation being seen in the hyper- and hypokinetic hemodynamic variants. The experimental studies have demonstrated a reverse relationship between the rise of LPO products in the blood, LPO activation in myocytes and the onset of arrhythmia because of an increase in the density of Na(+)-Ca++ channels of the membrane, accumulation of those ions by the cell, and an increase of the transmembranous potential at rest. Therefore, to prevent and treat cardiac rhythm impairment in CHD patients, it is necessary to administer the blockers of Na(+)-Ca++ channels (etmozin, ethacizine, verapamil and so forth) and antioxidants that raise the reserve capacity of the AOS, including laser therapy.

[Pathogenetic characteristics of the development of arrhythmia in patients with ischemic heart disease]. / Patogeneticheskie osobennosti vozniknoveniia narushenii serdechnogo ritma u bol'nykh IBS.

ACTH, hydrocortisone and cyclic nucleotides levels, beta-receptors sensitivity, lipid peroxidation (LPO), antioxidant system, hemodynamics were investigated in 93 coronary patients without myocardial infarction in the presence of cardiac arrhythmia and after its correction. The clinical and laboratory findings indicate that it is primarily LPO activation and LPO products excessive accumulation in the blood that are responsible for arrhythmia emergence. Experimental data support these results by showing possibility of cardiac arrhythmia onset due to LPO products which raise the density of Na-Ca channels and inhibit the activity of Ca-dependent ATPase.

[Biological effect of laser radiation on the functional state of cardiomyocytes]. / Biologicheskoe deistvie lazernogo izlucheniia na funktsional'noe sostoianie kardiomiotsitov.

Experiments were conducted on Wistar rats to study the effect of laser radiation (LR) with a wavelength of 632.8 and 890.0 nm and power of 0.0005-0.005 and 0.003-0.003 W/cm2, respectively, on the action potential (AP) and mechanical contraction of smooth muscle cells, as well as myocardial ATPase activity. LR with a wavelength of 632.8 and 890.0 nm in a dose of 3.0 J/cm2 activates Ca-ATP-ase activity, increases the activity of the myocardial antioxidant system, reduces the Na-Ca current through the membrane, and accelerates the return of Ca2+ into the sarcoplasmic reticulum. LR with a wavelength of 632.8 nm reduces mainly the Ca flow, while LR with a wavelength of 890.0 nm reduces the Na current In a dose of more than 3.0 J/cm2 LR causes a damaging effect manifested by increased Ca2+ current in the slow channels, increase of amplitude, duration of AP and repolarization phase. Synergism of the action of LR with a wavelength of 632.8 and 890.0 nm and that of some antiarrhythmic agents (procainamide, lidocaine, ethmosin, ethacisin, verapamil) was encountered.

[Comparative study of bovine adrenodoxin and renorenodoxin]. / Sravnitel'noe izuchenie adrenodoksina i renoredoksina byka.

The ferredoxin from bovine renal mitochondria (renoredoxin) has been obtained in a highly purified state. The A415/A280 ratio of the purified renoredoxin is 0.84. The absorption spectrum of renoredoxin was shown to be identical to that of bovine adrenodoxin. Two forms of renoredoxin (Mr 14200 and 13300) were detected by using polyacrylamide gel electrophoresis. These forms exhibit a very similar immunologic cross-reactivity with polyclonal antibodies to adrenodoxin. The N-terminal amino acid sequence of renal ferredoxin was shown to be identical to that of adrenodoxin; the C-terminal sequences of both ferredoxins undergo a similar post-translational proteolytic modification. The amino acid composition of ferredoxins are also very close. Renal ferredoxin can be replaced by adrenodoxin in reconstituted systems from bovine adrenal cortex mitochondria which catalyze the side chain cleavage of cholesterol to pregnenolone and the 11 beta-hydroxylation of deoxycorticosterone to corticosterone.