RESUMO
Retrospective testing of neonatal Guthrie card blood spots for specific immunoglobulin M (IgM) can distinguish congenital toxoplasmosis from acquired toxoplasmosis. We determined whether storage temperature reduced IgM detection, using filter paper blood samples "spiked" with anti-Toxoplasma IgM. After 300 days, IgM detection deteriorated with storage at room temperature but not at temperatures of 4 degrees C or lower.
Assuntos
Anticorpos Antibacterianos/imunologia , Sangue/imunologia , Imunoglobulina M/imunologia , Manejo de Espécimes/métodos , Toxoplasma/imunologia , Toxoplasmose/diagnóstico , Adolescente , Animais , Criança , Pré-Escolar , Dessecação , Humanos , Lactente , Recém-Nascido , Refrigeração , Temperatura , Fatores de TempoRESUMO
The seroprevalence of Toxocara in the Danish population was assessed from 3,247 sera from individuals originally screened for toxoplasmosis. Of 87 enzyme-linked immunosorbent assay-positive sera, 79 were confirmed by Western blotting, yielding a crude seroprevalence of 2.4%. This indicates that the seroprevalence of toxocariasis in Denmark is low compared to those in other European countries.