Phenol degradation and heavy metal tolerance of Antarctic yeasts.

In cold environments, biodegradation of organic pollutants and heavy metal bio-conversion requires the activity of cold-adapted or cold-tolerant microorganisms. In this work, the ability to utilize phenol, methanol and n-hexadecane as C source, the tolerance to different heavy metals and growth from 5 to 30 °C were evaluated in cold-adapted yeasts isolated from Antarctica. Fifty-nine percent of the yeasts were classified as psychrotolerant as they could grow in all the range of temperature tested, while the other 41% were classified as psychrophilic as they only grew below 25 °C. In the assimilation tests, 32, 78, and 13% of the yeasts could utilize phenol, n-hexadecane, and methanol as C source, respectively, but only 6% could assimilate the three C sources evaluated. In relation to heavy metals ions, 55, 68, and 80% were tolerant to 1 mM of Cr(VI), Cd(II), and Cu(II), respectively. Approximately a half of the isolates tolerated all of them. Most of the selected yeasts belong to genera previously reported as common for Antarctic soils, but several other genera were also isolated, which contribute to the knowledge of this cold environment mycodiversity. The tolerance to heavy metals of the phenol-degrading cold-adapted yeasts illustrated that the strains could be valuable as inoculant for cold wastewater treatment in extremely cold environments.

Bioprospection of cold-adapted yeasts with biotechnological potential from Antarctica.

The aim of this study was to investigate the ability to produce extracellular hydrolytic enzymes at low temperature of yeasts isolated from 25 de Mayo island, Antarctica, and to identify those exhibiting one or more of the evaluated enzymatic activities. A total of 105 yeast isolates were obtained from different samples and 66 were identified. They belonged to 12 basidiomycetous and four ascomycetous genera. Most of the isolates were ascribed to the genera Cryptococcus, Mrakia, Cystobasidium, Rhodotorula, Gueomyces, Phenoliferia, Leucosporidium, and Pichia. Results from enzymes production at low temperatures revealed that the Antarctic environment contains metabolically diverse cultivable yeasts, which represent potential tools for biotechnological applications. While most the isolates proved to produce 2-4 of the investigated exoenzymes, two of them evidenced the six evaluated enzymatic activities: Pichia caribbica and Guehomyces pullulans, which were characterized as psycrotolerant and psycrophilic, respectively. In addition, P. caribbica could assimilate several n-alkanes and diesel fuel. The enzyme production profile and hydrocarbons assimilation capacity, combined with its high level of biomass production and the extended exponential growth phase make P. caribbica a promising tool for cold environments biotechnological purposes in the field of cold-enzymes production and oil spills bioremediation as well.

Presence and distribution of persistent toxic substances in sediments and marine organisms of Potter Cove, Antarctica.

Levels of butyltin compounds, polychlorinated biphenyls, and heavy metals were analyzed in marine sediments and organisms (Notothenia coriiceps, Laternula elliptica, and Nacella concinna), each of which has a different feeding strategy, in Potter Cove, Antarctica. PCBs were lower than detection limits in all samples. Only butyltin compounds were detected in a restricted area near the scientific station. Chromium, copper, magnesium, lead (Pb), and zinc had similar behavior in the cove, probably because they are regulated by similar process and conditions. However, Pb levels in some sites of the cove seem to be related to human activities in the area. Cadmium levels were relatively low, with the highest values found close to the shoreline, which is probably influenced by seasonal streams draining waters from Potter Peninsula. Results showed that despite the fact that Jubany Station has been operating for > 50 years, surface sediment and biota from Potter Cove do not exhibit levels of pollutants representing environmental concern.