RESUMO
Epidermolysis bullosa simplex (EBS) is mainly caused by mutations in the KRT5 and KRT14 genes. Squamous cell carcinoma (SCC) represents the second most frequent skin neoplasia with complex aetiology. The molecular events disrupting the orchestrated interplay between the cytoskeleton, cell adhesion molecules and signalling proteins are ill understood in SCC. We describe the molecular background and the unusual course of the disease in a patient with EBS Dowling-Meara, severe keratoderma and a massive verrucous carcinoma. Skin and tumour samples from the patient were analysed using light microscopy, immunohistochemistry and immunofluorescence mapping. Mutation analysis of the KRT5 and KRT14 genes identified the novel KRT5 mutation p.E477D. Invasive tumour areas were characterized by downregulation of keratins 5 and 14, reduced and irregular desmocollin-2 expression and increased expression of keratins 6, 16 and 17. Levels of Ki-67 were increased and levels of E-cadherin strongly reduced in the tumour tissue. In this case a novel KRT5 mutation led to increased fragility of keratinocytes. Desmosome and adherens junctions were destabilized, which may trigger keratinocyte-mediated inflammation, possibly via p120-catenin-dependent signalling, suggesting a link between a keratin mutation and SCC, which adds weight to the hypothesis that disturbance of the cytoskeleton represents a major cause in the appearance of the malignant phenotype. Some individuals with EBS may be at risk of developing secondary SCC.
Assuntos
Carcinoma Verrucoso/complicações , Epidermólise Bolhosa Simples/complicações , Queratina-5/genética , Mutação/genética , Neoplasias Cutâneas/complicações , Adulto , Caderinas/metabolismo , Carcinoma Verrucoso/diagnóstico , Carcinoma Verrucoso/genética , Análise Mutacional de DNA , Regulação para Baixo , Epidermólise Bolhosa Simples/diagnóstico , Epidermólise Bolhosa Simples/genética , Técnica Indireta de Fluorescência para Anticorpo , Humanos , Imuno-Histoquímica , Queratina-14/genética , Queratinócitos/metabolismo , Queratinas/metabolismo , Antígeno Ki-67/metabolismo , Neoplasias Cutâneas/diagnóstico , Neoplasias Cutâneas/genética , Tomografia Computadorizada por Raios XRESUMO
Incidentally, a lesion in the right upper pulmonary lobe was found in a 74-year-old male patient. The flexible bronchoscopy revealed an endobrochial lipoma in the right lower lobe as the only pathological finding. Due to multiple co-morbidities, no intervention was performed initially. Since the endobronchial lipoma increased in size during follow-up, it was removed by cryoextraction. This case report on an endobronchial lipoma includes a short summary of this rare benign lung tumor.
Assuntos
Neoplasias Brônquicas/patologia , Neoplasias Brônquicas/cirurgia , Broncoscopia/métodos , Lipoma/patologia , Lipoma/cirurgia , Idoso , Humanos , Achados Incidentais , Masculino , Resultado do TratamentoRESUMO
BACKGROUND: Keratins are intermediate filament proteins of epithelial cells with pivotal functions for cell integrity. They comprise keratins 18 [K18] and 8 [K8] in hepatocytes. Keratins are of major importance for an intact cellular microarchitecture and have protective functions in human liver diseases. In mice, K8 has been demonstrated to protect against Fas-antibody-induced liver failure by direct interaction with apoptotic regulators, while the role of K18 remains unresolved. MATERIALS AND METHODS: We analysed effects of K18 deficiency on Fas-induced liver failure in mice. We determined survival and analysed induction of apoptosis after injection of the agonistic Fas antibody Jo2 into K18(-/-) and wild-type control mice by TUNEL assay and fluorometrically analysed caspase-3, -8 and -9 activities 1, 2 and 3 h after Jo2 injection. RESULTS: In K18(-/-) mice, survival of Fas-antibody treated mice was significantly shorter than that of wild-type controls (P = 0.02). However, shortened survival of K18(-/-) mice was caused by increased hepatic damage but was not correlated to enhanced induction of apoptotic pathways, as neither numbers of TUNEL positive apoptotic cells nor activities of caspases-3, -8 and -9 differed between K18(-/-) and K18(+/+) mice at any point of time. CONCLUSION: K18(-/-) mice are significantly more susceptible to Fas-antibody-induced liver failure. The cytoprotective effect of K18 is not explained by a differential activation of caspases-3, -8 and -9, suggesting that K18 does not directly interfere with apoptotic regulators. Importantly, however, K18 exerts significant protective functions by other mechanisms.
Assuntos
Anticorpos/metabolismo , Proteínas Reguladoras de Apoptose/metabolismo , Caspases/metabolismo , Queratina-18/metabolismo , Falência Hepática/metabolismo , Animais , Anticorpos/genética , Proteínas Reguladoras de Apoptose/genética , Caspases/genética , Células Cultivadas , Queratina-18/genética , Queratina-8 , Falência Hepática/genética , Camundongos , Transporte Proteico/genética , Transdução de Sinais/genéticaRESUMO
The roles of the different molecular domains of intermediate filament (IF) proteins in the assembly and higher order organization of IF structures have recently been studied by various groups but with partially controversial results. To examine the requirement of the aminoterminal (head) and the carboxyterminal (tail) domain of cytokeratins (CKs) for de novo IF formation in the living cell, we have constructed cDNAs coding for intact as well as head- and/or tail-less human CKs 8 and 18 and the naturally tail-less human CK 19, all under the control of the human beta-actin promoter. After transient and stable transfections of mouse 3T3-L1 cells, which are devoid of any CKs, we have studied, with such constructs, the resulting gene products by gel electrophoresis and immunolocalization techniques. By light and electron microscopy we show that extended cytoplasmic IF meshworks are formed from pairs of the type II CK 8 with the type I CKs 18 or 19 as well as from pairs of tail-less CK 8 with tail-less CKs 18 or 19 in the transfected cells, proving that the absence of the tail domain in both types of CKs does not prevent the de novo formation of regular IFs. Most surprisingly, however, we have observed spectacular alterations in the nucleocytoplasmic distribution of the IFs formed from tail-less CKs. In many of the transfected cells, a large part, or all, of the detectable CKs was found to occur in extensive IF bundles in the nucleoplasm. Intranuclear accumulations of CK deposits, however mostly nonfibrillar, were also observed when the cells had been transfected with cDNAs encoding tail-less CKs also lacking their head domains, whereas CKs deleted only in the head domain were found exclusively in the cytoplasm. The specific domain requirements for the assembly of cytoplasmic IF bundles are discussed and possible mechanisms of intranuclear accumulation of IFs are proposed.
Assuntos
Núcleo Celular/metabolismo , Citoplasma/metabolismo , Filamentos Intermediários/metabolismo , Queratinas/metabolismo , Células 3T3 , Sequência de Aminoácidos , Animais , Sequência de Bases , Núcleo Celular/ultraestrutura , Clonagem Molecular , Citoplasma/ultraestrutura , DNA , Imunofluorescência , Humanos , Camundongos , Microscopia Eletrônica , Dados de Sequência Molecular , TransfecçãoRESUMO
Tumor necrosis factor (TNF) is a cytokine produced by macrophages and T lymphocytes that acts through two distinct receptors, TNFR1 (60 kD, CD120a) and TNFR2 (80 kD, CD120b), to affect cellular proliferation, differentiation, survival, and cell death. In addition to its proinflammatory actions in mucosal tissue, TNF is important for liver regeneration. Keratin 8 (K8) and keratin 18 (K18) form intermediate filaments characteristic of liver and other single cell layered, internal epithelia and their derivative cancers. K8-deficient (K8(-)) mice, which escape embryonic lethality, develop inflammatory colorectal hyperplasia, mild liver abnormalities, and tolerate hepatectomy poorly. We show that normal and malignant epithelial cells deficient in K8 and K18 are approximately 100 times more sensitive to TNF-induced death. K8 and K18 both bind the cytoplasmic domain of TNFR2 and moderate TNF-induced, Jun NH(2)-terminal kinase (JNK) intracellular signaling and NFkappaB activation. Furthermore, K8(-) and K18(-) mice are much more sensitive to TNF dependent, apoptotic liver damage induced by the injection of concanavalin A. This moderation of the effects of TNF may be the fundamental function of K8 and K18 common to liver regeneration, inflammatory bowel disease, hepatotoxin sensitivity, and the diagnostic, persistent expression of these keratins in many carcinomas.
Assuntos
Apoptose/efeitos dos fármacos , Células Epiteliais/citologia , Células Epiteliais/efeitos dos fármacos , Queratinas/metabolismo , Fator de Necrose Tumoral alfa/farmacologia , Animais , Linhagem Celular , Doença Hepática Induzida por Substâncias e Drogas/patologia , Concanavalina A/toxicidade , Células Epiteliais/metabolismo , Células Epiteliais/patologia , Humanos , Proteínas Quinases JNK Ativadas por Mitógeno , Queratinas/deficiência , Queratinas/genética , Fígado/citologia , Fígado/efeitos dos fármacos , Fígado/metabolismo , Fígado/patologia , Camundongos , Camundongos Knockout , Proteínas Quinases Ativadas por Mitógeno/metabolismo , NF-kappa B/genética , NF-kappa B/metabolismo , Ligação Proteica , Receptores do Fator de Necrose Tumoral/genética , Receptores do Fator de Necrose Tumoral/metabolismo , Transdução de Sinais/efeitos dos fármacos , Transfecção , Células Tumorais CultivadasRESUMO
Bullous congenital ichthyosiform erythroderma (BCIE) is a dominantly inherited blistering skin disorder caused by point mutations in the suprabasal cytokeratins 1 or 10. Targeting the murine cytokeratin 10 gene in ES cells resulted in mice with different phenotypes in the homozygotes and heterozygotes; both of which exhibit similarities to specific clinical characteristics of BCIE. Homozygotes suffered from severe skin fragility and died shortly after birth. Heterozygotes were apparently unaffected at birth, but developed hyperkeratosis with age. In both genotypes, aggregation of cytokeratin intermediate filaments, changes in cytokeratin expression, and alterations in the program of epidermal differentiation were observed. In addition we demonstrate, for the first time, the existence of the murine equivalent of human cytokeratin 16.
Assuntos
Modelos Animais de Doenças , Hiperceratose Epidermolítica/genética , Queratinas/genética , Animais , Sequência de Bases , Marcação de Genes , Genes Letais , Heterozigoto , Homozigoto , Hiperceratose Epidermolítica/etiologia , Hiperceratose Epidermolítica/terapia , Queratina-10 , Queratinas/análise , Queratinas/deficiência , Queratinas/ultraestrutura , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Endogâmicos CBA , Camundongos Knockout , Camundongos Transgênicos , Dados de Sequência Molecular , Pele/patologiaRESUMO
Here, we report on the analysis of keratin 18 null mice. Unlike the ablation of K8, which together with K18 is expressed in embryonic and simple adult epithelia, K18 null mice are viable, fertile, and show a normal lifespan. In young K18 null mice, hepatocytes were completely devoid of keratin filaments. Nevertheless, typical desmosomes were formed and maintained. Old K18 null mice, however, developed a distinctive liver pathology with abnormal hepatocytes containing K8-positive aggregates. These stained positively for ubiquitin and MM120-1 and were identified as Mallory bodies, one hallmark of human alcoholic hepatitis. This is the first demonstration that the ablation of one keratin leads to the accumulation of its single partner. Another striking finding was the absence or drastic down regulation of K7 in several tissues despite its ongoing transcription. Moreover, K18 null mice revealed new insights in the filament-forming capacity of the tail-less K19 in vivo. Due to the unexpected secondary loss of K7, only K8/19 are expressed in the uterine epithelium of K18 null mice. Immunoelectron microscopy of this tissue demonstrated the presence of typical K8/19 IF, thus highlighting in vivo that K19 is a fully competent partner for K8.
Assuntos
Células Epiteliais/química , Filamentos Intermediários/metabolismo , Queratinas/genética , Queratinas/metabolismo , Fatores Etários , Animais , Anticorpos Monoclonais , Desmossomos/fisiologia , Desmossomos/ultraestrutura , Células Epiteliais/citologia , Células Epiteliais/fisiologia , Fertilidade , Expressão Gênica , Heterozigoto , Homozigoto , Filamentos Intermediários/química , Filamentos Intermediários/imunologia , Queratina-7 , Queratinas/imunologia , Expectativa de Vida , Fígado/química , Fígado/patologia , Camundongos , Camundongos Knockout , Microscopia Imunoeletrônica , Mutagênese/genética , Estrutura Terciária de Proteína , RNA Mensageiro/metabolismo , Células-Tronco/química , Células-Tronco/citologia , Células-Tronco/fisiologiaRESUMO
BACKGROUND: Connexins are the protein subunits of intercellular gap junction channels. In mammals, they are encoded by a family of at least 15 genes, which show cell-type-specific but overlapping patterns of expression. Mice lacking connexin43 (Cx43) die postnatally from obstruction of the right ventricular outflow tract of the heart. To discriminate between the unique and shared functions of Cx43, Cx40 and Cx32, we generated two 'knock-in' mouse lines, Cx43KI32 and Cx43KI40, in which the coding region of the Cx43 gene was replaced, respectively, by the coding regions of Cx32 or Cx40. RESULTS: Heterozygous mutants were fertile and co-expressed the wild-type and the corresponding recombinant allele in all tissues analyzed. Heterozygous Cx43KI32, but not Cx43KI40, mutant mothers were unable to nourish their pups to weaning age, possibly reflecting a defect in milk ejection. Homozygous mutant males were sterile because of extensive germ-cell deficiency. The ovaries of homozygous Cx43KI32 neonates exhibited all stages of follicular development and ovulation. The hearts of homozygous Cx43KI32 neonates showed mild morphological defects, but the cardiac morphology of homozygous Cx43KI40 neonates was relatively normal. Spontaneous ventricular arrhythmias were observed in most Cx43KI40 and some Cx43KI32 mutant mice, suggesting increased ventricular vulnerability in these mice. CONCLUSIONS: The postnatal lethality of Cx43-deficient mice was rescued in Cx43KI32 or Cx43KI40 mice, indicating that Cx43, Cx40 and Cx32 share at least some vital functions. On the other hand, Cx43KI32 and Cx43KI40 mice differed functionally and morphologically from each other and from wild-type mice. Thus, these connexins also have unique functions.
Assuntos
Conexina 43/fisiologia , Conexinas/fisiologia , Junções Comunicantes/metabolismo , Animais , Arritmias Cardíacas/fisiopatologia , Conexina 43/genética , Conexinas/genética , Eletrocardiografia , Feminino , Junções Comunicantes/genética , Marcação de Genes , Genótipo , Coração/fisiologia , Infertilidade/fisiopatologia , Masculino , Glândulas Mamárias Animais/anatomia & histologia , Glândulas Mamárias Animais/fisiologia , Camundongos , Morfogênese , Mutagênese Sítio-Dirigida , Miocárdio/metabolismo , Miocárdio/patologia , Testículo/crescimento & desenvolvimento , Testículo/patologia , Transgenes , Proteína beta-1 de Junções Comunicantes , Proteína alfa-5 de Junções ComunicantesRESUMO
The expression of distinct keratin pairs during epidermal differentiation is assumed to fulfill specific and essential cytoskeletal functions. This is supported by a great variety of genodermatoses exhibiting tissue fragility because of keratin mutations. Here, we show that the loss of K10, the most prominent epidermal protein, allowed the formation of a normal epidermis in neonatal mice without signs of fragility or wound-healing response. However, there were profound changes in the composition of suprabasal keratin filaments. K5/14 persisted suprabasally at elevated protein levels, whereas their mRNAs remained restricted to the basal keratinocytes. This indicated a novel mechanism regulating keratin turnover. Moreover, the amount of K1 was reduced. In the absence of its natural partner we observed the formation of a minor amount of novel K1/14/15 filaments as revealed by immunogold electron microscopy. We suggest that these changes maintained epidermal integrity. Furthermore, suprabasal keratinocytes contained larger keratohyalin granules similar to our previous K10T mice. A comparison of profilaggrin processing in K10T and K10(-/-) mice revealed an accumulation of filaggrin precursors in the former but not in the latter, suggesting a requirement of intact keratin filaments for the processing. The mild phenotype of K10(-/-) mice suggests that there is a considerable redundancy in the keratin gene family.
Assuntos
Epiderme/metabolismo , Queratinas/biossíntese , Queratinas/genética , Animais , Animais Recém-Nascidos , Northern Blotting , Western Blotting , Eletroforese em Gel Bidimensional , Epiderme/ultraestrutura , Proteínas Filagrinas , Imuno-Histoquímica , Hibridização In Situ , Proteínas de Filamentos Intermediários/química , Queratina-10 , Queratina-14 , Queratina-15 , Queratina-5 , Camundongos , Camundongos Knockout , Microscopia Eletrônica , Microscopia de Fluorescência , Família Multigênica , Precursores de Proteínas/química , RNA Mensageiro/metabolismo , CicatrizaçãoRESUMO
In human patients, a wide range of mutations in keratin (K) 5 or K14 lead to the blistering skin disorder epidermolysis bullosa simplex. Given that K14 deficiency does not lead to the ablation of a basal cell cytoskeleton because of a compensatory role of K15, we have investigated the requirement for the keratin cytoskeleton in basal cells by inactivating the K5 gene in mice. We report that the K5(-/-) mice die shortly after birth, lack keratin filaments in the basal epidermis, and are more severely affected than K14(-/-) mice. In contrast to the K14(-/-) mice, we detected a strong induction of the wound-healing keratin K6 in the suprabasal epidermis of cytolyzed areas of postnatal K5(-/-) mice. In addition, K5 and K14 mice differed with respect to tongue lesions. Moreover, we show that in the absence of K5 and other type II keratins, residual K14 and K15 aggregated along hemidesmosomes, demonstrating that individual keratins without a partner are stable in vivo. Our data indicate that K5 may be the natural partner of K15 and K17. We suggest that K5 null mutations may be lethal in human epidermolysis bullosa simplex patients.
Assuntos
Epidermólise Bolhosa Simples/genética , Queratinas/genética , Queratinas/metabolismo , Pele/metabolismo , Animais , Southern Blotting , Modelos Animais de Doenças , Eletroforese em Gel Bidimensional , Epidermólise Bolhosa Simples/metabolismo , Deleção de Genes , Vetores Genéticos , Humanos , Queratina-14 , Queratina-15 , Queratina-5 , Queratinas/biossíntese , Camundongos , Camundongos Knockout , Microscopia Eletrônica , Microscopia de Fluorescência , Modelos Genéticos , Mutação , Fenótipo , Reação em Cadeia da Polimerase , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Pele/ultraestrutura , Fenômenos Fisiológicos da Pele , Fatores de TempoRESUMO
Cytokeratins are a family of polypeptides that form the intermediate-sized filament characteristic of epithelial cells. The cytoskeletons of different types of epithelial cells have been reported to possess specific combinations of the members of this protein family. Therefore, we have sought to examine the correspondence between such differential protein expression and the expression of cytokeratin genes at the nucleic acid level. A library of recombinant plasmids carrying cDNA sequences synthesized from bovine epidermal mRNAs was constructed. Clones of about 10(3) base-pairs coding for all the major epidermal keratins of molecular weights of 50,000, 54,000, 59,000, 60,000 and 68,000 were identified by means of hybridization-selection, followed by one and two-dimensional gel electrophoresis of products of translation in vitro. Under stringent conditions, each of these clones hybridizes specifically with its corresponding mRNA and does not show significant cross-hybridization with mRNAs coding for the other keratins, including those belonging to the same subfamily. Using these clones in RNA blot hybridization analysis, we have studied the expression of keratin genes in diverse bovine epithelial tissues (muzzle epidermis, cornea, esophagus, bladder urothelium, liver) and cultured cell lines from kidney (MDBK) and mammary gland (BMGE + H, BMGE -H). In each case we have found a correlation between the respective keratin polypeptides and the corresponding mRNAs. Whereas mRNA coding for keratins Ia and VIb have been found only in epidermis, genes coding for other epidermal keratins are expressed also in certain non-epidermal epithelia and in cells of the BMGE + H line. In contrast, epidermal keratin mRNA sequences have not been detected in liver or bladder tissue, nor in cultured kidney cells (MDBK) or mammary gland cells of the BMGE - H line, which all express a set of cytokeratin polypeptides entirely different from those of epidermis. In all cases, only one mRNA size species has been found, suggesting that in different cell types the same mRNA species is synthesized from the same keratin gene. We conclude that the mechanisms controlling the cell type-specific synthesis of the diverse keratin genes act at a pre-translational level.
Assuntos
DNA/genética , Regulação da Expressão Gênica , Queratinas/genética , Animais , Bovinos , Células Cultivadas , Clonagem Molecular , Eletroforese em Gel de Ágar , Células Epidérmicas , Epiderme/análise , Hibridização de Ácido Nucleico , Biossíntese de Proteínas , RNA Mensageiro/genética , Distribuição TecidualRESUMO
We have studied the formation of new intermediate-sized filaments (IFs) by human cytokeratins (CKs) 8 and/or 19 in cultured bovine lens cells stably transfected with the corresponding cDNAs under SV40 promoter control. In the transfected cells, polypeptides of both type I and type II CKs were synthesized to near-equimolar amounts, formed heterotypic complexes and assembled into IFs with a peculiar tendency to accumulate into variously sized, often roundish aggregates in the juxtanuclear region, usually one per cell. Electron microscopy of these large CK IF aggregates revealed typical 7 to 12-nm IFs, tightly packed together in an apparently haphazard mode. By immunoelectron microscopy, the CK IFs could be readily distinguished from the vimentin IFs which were abundant in these cells. Electron microscopy also showed that many of the CK IF aggregates were located in the vicinity of the nucleus but did not have direct contact with the nuclear envelope; moreover, their location did not regularly correspond to those of the centrosomes and the Golgi apparatus. During enucleation of transfected cells in the presence of cytochalasin B, the CK aggregates were often retained in the cytoplast. After microinjection of CK 8 and 19 mRNAs, synthesized in vitro from cDNA molecules, into enucleated cytoplasts prepared from untransfected cells, CK IFs similar to those observed in microinjected whole cells were formed but often showed a wider cytoplasmic distribution. Our observations indicate that typical CK IFs can form, in vivo, in the absence of any nuclear structures. We discuss possible reasons for the tendency of the CK IFs to accumulate, in this cell line, into a juxtanuclear aggregate, in relation to similar CK-IF aggregates formed in certain normal cell types and upon toxic damages.
Assuntos
Queratinas/genética , Cristalino/metabolismo , Transfecção , Animais , Northern Blotting , Bovinos , DNA/genética , Imunofluorescência , Humanos , Queratinas/biossíntese , Queratinas/metabolismo , Microinjeções , Microscopia Eletrônica , RNA Mensageiro/genéticaRESUMO
Plectin/HD1 is a high molecular weight protein (approximately 500 kDa) that has been proposed to act as an important and versatile cytoskeletal cross-linker molecule. Mutations of the human plectin gene have recently been associated with the autosomal recessive disorder epidermolysis bullosa simplex with muscular dystrophy. We studied the expression of plectin/HD1 in various neuromuscular disorders by indirect immunofluorescence. In cross sections of normal human muscle, plectin/HD1 showed a checkerboard-like distribution with moderate to intense cytoplasmic and sarcolemmal staining in type 1 fibers and a faint staining of the sarcolemma in type 2 fibers. In longitudinal sections of plectin/HD1-positive fibers a cross-striation staining pattern was noted. This fiber type-related expression was significantly altered in the group of dystrophinopathies, whereas it was maintained in all other myopathies and denervating disorders. In seven dystrophinopathies studied, a markedly increased plectin/HD1 immunoreactivity at the sarcolemmal level of type 2 fibers was observed. Confocal laser microscopy of normal skeletal muscle revealed a colocalization of desmin and plectin/HD1 at the level of the sarcolemma. This suggests that plectin/HD1- in analogy to its demonstrated involvement in cytokeratin-hemidesmosome linkage in epidermis-may mediate the anchorage of desmin to the sarcolemma (i.e. to costameres).
Assuntos
Proteínas de Filamentos Intermediários/metabolismo , Doenças Neuromusculares/metabolismo , Southern Blotting , Western Blotting , Genes , Humanos , Proteínas de Filamentos Intermediários/análise , Proteínas de Filamentos Intermediários/genética , Microscopia Confocal , Microscopia de Fluorescência , Músculo Esquelético/química , Músculo Esquelético/ultraestrutura , Distrofias Musculares/metabolismo , PlectinaRESUMO
Plectin is a multifunctional cytoskeletal linker protein with an intermediate filament-binding site and sequence elements with high homology to actin-binding domains. Mutations of the human plectin gene as well as the targeted inactivation of its murine analog cause a generalized blistering skin disorder and muscular dystrophy, thus implying its essential role in cells that are exposed to mechanical stress. In the present study we report the characterization of two new domain-specific plectin antibodies as well as ultrastructural localization of plectin in normal human skeletal muscle. Using immunogold electron microscopy, we localized plectin at three prominent sites: 1) Plectin is found at regularly spaced intervals along the cytoplasmic face of the plasma membrane. 2) It is distinctly localized at filamentous bridges between Z-lines of peripheral myofibrils and the sarcolemma and 3) at structures forming the intermyofibrillar scaffold. At the latter two locations, plectin and desmin were found to colocalize. Our ultrastructural analysis suggests that plectin may have a central role in the structural and functional organization of the intermediate filament cytoskeleton in mature human skeletal muscle.
Assuntos
Citoesqueleto/metabolismo , Desmina/fisiologia , Imuno-Histoquímica , Proteínas de Filamentos Intermediários/fisiologia , Músculo Esquelético/metabolismo , Células 3T3 , Animais , Desmina/análise , Células HeLa , Humanos , Proteínas de Filamentos Intermediários/análise , Camundongos , Músculo Esquelético/anatomia & histologia , Plectina , Células Tumorais CultivadasRESUMO
Ankyrins represent a protein family whose members are associated with membrane proteins and the actin cytoskeleton. The principal ankyrin domain structure comprises an amino-terminal membrane-binding, a spectrin-binding, and a regulatory domain, and can be modulated by alternative splicing. In order to investigate the role of ankyrin-3 in skin, we have isolated three complete ankyrin-3 cDNA clones of 5.8 kb, 5.2 kb, and 2.5 kb by reverse transcription-polymerase chain reaction of mouse skin RNA. DNA sequencing confirmed the isolated clones to be splice variants of ankyrin-3. Of these, the smallest cDNA represents a novel ankyrin named ankyrin-3(93). Surprisingly, this novel ankyrin subtype lacks not only all ankyrin repeats, but also the first 75 amino acids of the spectrin-binding domain. Immuno-fluorescence analysis of mouse skin showed that ankyrin-3 is expressed in all living layers of mouse epidermis. Here, it predominates along the basal and lateral membranes of the basal layer in addition to an even cytoplasmic distribution. In primary mouse keratinocytes grown at elevated Ca2+ levels, ankyrin-3(93) was localized along the plasma membrane and throughout the cell in a Golgi-like fashion. Depending on fixation conditions, nuclear staining became apparent in many cells. In agreement with previous data, northern blotting revealed a widespread distribution of the two larger ankyrin splice variants. In contrast, the mRNA coding for ankyrin-3(93)was restricted to mouse skin. Reverse transcription-polymerase chain reaction of mouse skin RNA strongly suggested additional ankyrin isoforms in skin. Our data on ankyrin-3(93), which lacks a part of the spectrin-binding domain that regulates the affinity to spectrin, suggests a new function for this member of the ankyrin family.
Assuntos
Anquirinas/genética , Sequência de Aminoácidos , Animais , Anquirinas/metabolismo , Sequência de Bases , Variação Genética , Queratinócitos/química , Camundongos , Dados de Sequência Molecular , Isoformas de Proteínas/metabolismo , Processamento de Proteína , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Pele/ultraestrutura , Frações Subcelulares/químicaRESUMO
Epidermal thickening is a phenomenon common to many genodermatoses but little is known about the underlying causes. We have recently created a mouse model for the human skin disease bullous congenital ichthyosiform erythroderma by gene targeting. Mice heterozygous for a truncated keratin 10 gene exhibit acanthosis and hyperkeratosis as seen in the human disease. The degree of epidermal thickening is highly variable, offering a novel opportunity to investigate how epidermal homeostasis is modulated in keratin disorders by comparing epidermis from different body regions. We have performed bromodeoxyuridine labeling experiments and detected proliferation antigens by immunohistochemical means to compare proliferation in the epidermis of wild-type and heterozygous mice. These results have been compared with the expression of epidermal differentiation markers and of the "hyperproliferation associated" keratins K6 and K16. These experiments indicated that hyperproliferation is only partly responsible for the morphologic changes and that other mechanisms such as decreased desquamation are likely to be involved.
Assuntos
Epiderme/fisiopatologia , Hiperceratose Epidermolítica/fisiopatologia , Dermatopatias/fisiopatologia , Animais , Dorso , Biomarcadores/análise , Divisão Celular/genética , Divisão Celular/fisiologia , Modelos Animais de Doenças , Orelha , Epiderme/química , Epiderme/patologia , Esôfago , Pé , Expressão Gênica/genética , Histocitoquímica , Hiperceratose Epidermolítica/genética , Imuno-Histoquímica , Integrina beta1/genética , Queratinas/análise , Queratinas/genética , Antígeno Ki-67/análise , Camundongos , Camundongos Knockout , Antígeno Nuclear de Célula em Proliferação/análise , Pele/química , Pele/patologia , Pele/fisiopatologia , Dermatopatias/genéticaRESUMO
The gene (hprt) coding for mouse HPRT (hypoxanthine phosphoribosyltransferase) is transcribed from a promoter lacking CAAT and TATAA boxes. It is expressed ubiquitously, albeit at different levels, in all tissues and cultured cells. During investigations to characterise hprt transcription control elements required in embryonic stem (ES) cells and to develop compact hprt minigenes for gene-targeting strategies, we discovered a requirement for intron-1 sequences for expression in ES cells. The essential intron-1 element, which is 420 bp long, is located 230 bp downstream from the transcription start point and is shown to increase transcription from the hprt promoter in a position- and orientation-dependent manner. We propose that this element is an integral downstream part of the hprt promoter.
Assuntos
Regulação da Expressão Gênica , Hipoxantina Fosforribosiltransferase/genética , Íntrons , Sequências Reguladoras de Ácido Nucleico , Animais , Sequência de Bases , Células Cultivadas , DNA , Embrião de Mamíferos , Camundongos , Dados de Sequência Molecular , Reação em Cadeia da Polimerase , Regiões Promotoras Genéticas , RNA Mensageiro/metabolismo , TransfecçãoRESUMO
Cytokeratins are a large multigene family comprising two polypeptide types, i.e. acidic (type I) and basic (type II) ones, which are distinguished on the basis of immunological, peptide mapping, mRNA hybridization, and primary amino acid sequence data. The acidic (type I) cytokeratins can be subdivided into at least two different subtypes on the basis of their carboxy-terminal sequences. Considerable interspecies conservation of sequences exists, even extending to the 3'-non-coding mRNA regions. Different pairs of type I and II cytokeratins show different resistance to dissociation in urea. Sequence differences of the type I cytokeratins containing functional domains may be an explanation of the observed preference of co-expression with certain type II cytokeratins. The distribution of the different type I and II cytokeratins in normal epithelia and in carcinomas is differentiation related and can be used for cell typing and identification. The cell type-specific expression of cytokeratin polypeptides is recognized at both the protein and the mRNA level. The building block of cytokeratin IFs is a heterotypic tetramer, consisting of two type I and two type II polypeptides arranged in pairs of laterally aligned coiled coils. This principle of tetrameric organization is thought to be generally applicable to IFs.