Biological production of xylitol by using nonconventional microbial strains.

Xylitol (C5H12O5), an amorphous sugar alcohol of crystalline texture has received great interest on the global market due to its numerous applications in different industries. In addition to its high anticariogenic and sweetening properties, characteristics such as high solubility, stability and low glycemic index confer xylitol its fame in the food and odontological industries. Moreover, it also serves as a building-block in the production of polymers. As a result of the harmful effects of the chemical production of xylitol, the biotechnological means of producing this polyol have evolved over the decades. In contrast to the high consumption of energy, long periods of purification, specialized equipment and high production cost encountered during its chemical synthesis, the biotechnological production of xylitol offers advantages both to the economy and the environment. Non-Saccharomyces yeast strains, also termed as nonconventional, possess the inherent capacity to utilize D-xylose as a sole carbon source, unlike Saccharomyces species.

The draft genome sequence of Rhodosporidium toruloides strain Z11, an isolate capable of co-producing lipids and carotenoids from waste molasses.

A wild-type Rhodosporidium toruloides strain Z11 which could utilize molasses to co-produce high amount of lipid and carotenoids was isolated and characterized. The genome of strain Z11 with a G + C content of 59.0% was estimated to be 22.6 Mb and contained 5290 encoded protein sequences. Among these annotated genes, the ATP citrate (pro-S)-lyase, two malic enzymes (MaeA and MaeB) and the geranylgeranyl pyrophosphate synthase play key roles for the production of lipids and carotenoids. In addition, a ß-fructofuranosidase (SacA) was identified, which may contribute to the utilization of molasses.

Applications of synthetic light-driven microbial consortia for biochemicals production.

Synthetic microbial consortia provide a versatile and efficient platform for biochemicals production through the labor division. Especially, microbial communities composed of phototrophs and heterotrophs offer a promising alternative, as they can directly convert carbon dioxide (CO2) into chemicals. Within this system, photoautotrophic microbes can convert CO2 into organic carbon for microbial growth and metabolites synthesis by the heterotrophic partners. In return, heterotrophs can provide additional CO2 to support the growth of photoautotrophic microbes. However, the unmatched growing conditions, low stability and production efficiency of synthetic microbial consortia hinder their further applications. Thus, design and construction of mutualistic and stable synthetic light-driven microbial consortia are urgently needed. In this review, the progress of synthetic light-driven microbial consortia for chemicals production was comprehensively summarized. In addition, space-efficient synthetic light-driven microbial consortia in hydrogel system were reviewed. Perspectives on orderly distribution of light-driven microbial consortia associated with 3D printing technology in biomanufacturing were also addressed.