Highly sensitive paper-based electrochemical sensor for reagent free detection of bisphenol A.

Bisphenol A is one the most relevant endocrine disruptors for its toxicity and ubiquity in the environment, being largely employed as raw material for manufacturing processes of a wide number of compounds. Furthermore, bisphenol A is released in the drinking water when plastic-based bottles are incorrectly transported under sunlight, delivering contaminated drinking water. For the health of human beings and the environment, rapid and on site detection of bisphenol A in drinking water is an important issue. Herein, we report a novel and cost-effective printed electrochemical sensor for an enzymatic-free bisphenol A detection. This sensor encompasses the entire electrochemical cell printed on filter paper and the reagents for the measurement loaded in the cellulose fiber network, for delivering a reagent-free analytical tool. The working electrode was printed using ink modified with carbon black, a cost effective nanomaterial for sensitive and sustainable bisphenol A determination. Several parameters including pH, frequency, and amplitude were optimized allowing for a detection limit of 0.03 µM with two linear ranges 0.1-0.9 µM and 1 µM-50 µM, using square wave voltammetry as electrochemical technique. The satisfactory recovery values found in river and drinking water samples demonstrated the suitability of this sensor for screening analyses in water samples. These results revealed the attractiveness of this paper-based device thanks to the synergic combination of paper and carbon black as cost-effective materials.

A label-free impedimetric aptasensor for the detection of Bacillus anthracis spore simulant.

Herein, we report an impedimetric DNA-based aptamer sensor for a single-step detection of B. anthracis spore simulant (B. cereus spore). Specifically, we designed a miniaturized label-free aptasensor for B. cereus spores based on a gold screen-printed electrode functionalized with B. cereus spores-binding aptamer (BAS-6R). Several parameters were optimized to fabricate the aptasensor such as the concentration of DNA aptamer solution (0.5 µM), the time (48 h), the temperature (4 °C), and the pH (7.5) for aptamer immobilization on the working electrode surface. Once the aptasensor was developed, it was tested against B. cereus spores 14579 evaluating the effect of incubation time and MgCl2 concentration. Under the optimized conditions (incubation time equal to 3 h and absence of MgCl2), B. cereus spores 14579 were detected with a linear range between 104 CFU/ml and 5 × 106 CFU/ml and a detection limit of 3 × 103 CFU/ml. Furthermore, the study of selectivity toward B. cereus 11778, B. subtilis, Legionella pneumophila, and Salmonella Typhimurium has demonstrated the capability of this sensor to detect B. cereus spores, proving the suitability of the DNA-based sensing element combined with a portable instrument for a label-free measurement on site of B. anthracis spore simulant.