Identification of the Incisive Branch of the Inferior Alveolar Nerve of Edentulous Mandibles Using Cone Beam Computed Tomography.

This study explored the average length of the incisive branch of the inferior alveolar nerve on cone-beam computed tomography (CBCT) regarding patient demographics in patients with edentulous mandibles. CBCT was utilized in a retrospective study of edentulous mandibles to assess the presence and anatomical variation for the incisive branch (IB). Three independent observers measured bilateral IB lengths. In addition to demographics, IB length and port of exit data were obtained. A one-way ANOVA was used to test IB length varied by sex or port of exit, and a standard Pearson's correlation was used to test for IB length and age significance with a significance level of a p-value<0.05. Intraclass correlation coefficients show significant agreement in IB length across all observers. No significant difference was noted between the exit port and IB length. An important effect was reported for sex, indicating women have generally shorter IB lengths (9.43 ± 3.99 vs 10.55 ± 3.92). There was a significant correlation with age, but the relationship was weak. Edentulous mandibles have an altered anatomic landscape and establishing predictive incisive branch dimensions aids practitioners in surgical planning.

Circulating tumor DNA-based molecular residual disease detection for treatment monitoring in advanced melanoma patients.

BACKGROUND: Immune checkpoint inhibitors (ICIs) have substantially improved overall survival in patients with advanced melanoma; however, the lack of biomarkers to monitor treatment response and relapse remains an important clinical challenge. Thus, a reliable biomarker is needed that can risk-stratify patients for disease recurrence and predict response to treatment. METHODS: A retrospective analysis using a personalized, tumor-informed circulating tumor DNA (ctDNA) assay on prospectively collected plasma samples (n = 555) from 69 patients with advanced melanoma was performed. Patients were divided into three cohorts: cohort A (N = 30), stage III patients receiving adjuvant ICI/observation; cohort B (N = 29), unresectable stage III/IV patients receiving ICI therapy; and cohort C (N = 10), stage III/IV patients on surveillance after planned completion of ICI therapy for metastatic disease. RESULTS: In cohort A, compared to molecular residual disease (MRD)-negative patients, MRD-positivity was associated with significantly shorter distant metastasis-free survival (DMFS; hazard ratio [HR], 10.77; p = .01). Increasing ctDNA levels from the post-surgical or pre-treatment time point to after 6 weeks of ICI were predictive of shorter DMFS in cohort A (HR, 34.54; p < .0001) and shorter progression-free survival (PFS) in cohort B (HR, 22; p = .006). In cohort C, all ctDNA-negative patients remained progression-free for a median follow-up of 14.67 months, whereas ctDNA-positive patients experienced disease progression. CONCLUSION: Personalized and tumor-informed longitudinal ctDNA monitoring is a valuable prognostic and predictive tool that may be used throughout the clinical course of patients with advanced melanoma.

Increased drought severity tracks warming in the United States' largest river basin.

Across the Upper Missouri River Basin, the recent drought of 2000 to 2010, known as the "turn-of-the-century drought," was likely more severe than any in the instrumental record including the Dust Bowl drought. However, until now, adequate proxy records needed to better understand this event with regard to long-term variability have been lacking. Here we examine 1,200 y of streamflow from a network of 17 new tree-ring-based reconstructions for gages across the upper Missouri basin and an independent reconstruction of warm-season regional temperature in order to place the recent drought in a long-term climate context. We find that temperature has increasingly influenced the severity of drought events by decreasing runoff efficiency in the basin since the late 20th century (1980s) onward. The occurrence of extreme heat, higher evapotranspiration, and associated low-flow conditions across the basin has increased substantially over the 20th and 21st centuries, and recent warming aligns with increasing drought severities that rival or exceed any estimated over the last 12 centuries. Future warming is anticipated to cause increasingly severe droughts by enhancing water deficits that could prove challenging for water management.

Hydraulic traits of co-existing conifers do not correlate with local hydroclimate condition: a case study in the northern Rocky Mountains, U.S.A.

In this study, we examined the inter- and intra-specific variation of hydraulic traits of three conifers of the Northern Rockies: Pinus ponderosa, Picea engelmannii, and Pseudotsuga menziesii to understand the mechanisms that allow different plant species to co-exist across a watershed. We quantified differences in plant xylem water potential (ψx), xylem tissue vulnerability to cavitation (P50, or ψ causing 50% loss of hydraulic conductivity), and safety margins for co-occurring trees from low and high elevations. We then investigated xylem vulnerability to cavitation with rooting depth. We found that xylem vulnerability to cavitation did not correspond to where tree species were found in the landscape. For example, P. ponderosa grew in more xeric locations, while P. engelmannii were largely confined to more mesic locations, yet P. engelmannii had more negative P50 values. P. menziesii had the lowest P50 value, but displayed little variation in vulnerability to cavitation across the hydroclimatic gradient. These patterns were also reflected in the safety margins; P. menziesii had the widest safety margin, P. engelmannii was intermediate, and P. ponderosa displayed the narrowest safety margin. All three species were also using water sources deeper than 30 cm in depth, allowing them to persist throughout the mid-summer drought. Overall, species-specific hydraulic traits did not necessarily follow a predictable response to the environment; instead, a combination of physiological and morphological traits likely allow trees to persist across a broader hydroclimatic gradient than would be otherwise expected from hydraulic trait measurements alone.

The Value of Detecting Pathological Changes During Clot Formation in Early Disease Treatment-Naïve Breast Cancer Patients.

Breast cancer (BC) is one of the most prevalent forms of cancer in women worldwide. Clinical research indicates that BC patients are at an increased risk for thrombotic events, drastically decreasing their quality-of-life and treatment outcomes. There is ample evidence of this in the literature, but it is mainly focused on metastatic BC. Therefore, coagulopathies of nonmetastatic BC are understudied and require in-depth investigation. In this study, clot kinetics and ultrastructure were used to investigate treatment-naïve, nonmetastatic BC patients using scanning electron microscopy, Thromboelastography®, and confocal laser scanning microscopy. It was demonstrated that nonmetastatic BC patients exhibit minimal ultrastructural alterations of the clot components and no changes in the clot kinetics. However, BC patients presented changes to fibrinogen protein structure, compared to matched controls, using an amyloid-selective stain. Together, these findings suggest that coagulation dysfunction(s) in BC patients with early disease manifest at the microlevel, rather than the macrolevel. This study presents novel insights to a method that are more sensitive to coagulation changes in this specific patient group, emphasizing that the coagulation system may react in different forms to the disease, depending on the progression of the disease itself.

Why do children punish? Fair outcomes matter more than intent in children's second- and third-party punishment.

Humans punish fairness violations both as victims and as impartial third parties, which can maintain cooperative behavior. However, it is unknown whether similar motivations underlie punishment of unfairness in these two contexts. Here we approached this question by focusing on how both types of punishment develop in children, asking the question: What motivates young children to punish in response to fairness norm violations? We explored two potential factors: the direct experience of unfair outcomes and a partner's fair versus unfair intentions. The participants, 5- and 7-year-olds, were given the chance to engage in both second- and third-party punishment in response to either intended or unintended fairness norm violations in a single paradigm. In both age-groups, children were more likely to punish when they were directly affected by the allocation (second-party punishment) than when they were an uninvolved third party (third-party punishment). Reliable third-party punishment was shown only in the older age-group. Moreover, children's punishment was driven by outcome rather than intent, with equal rates of punishment when unequal outcomes were either the result of chance or the intentional act of another child. These findings suggest that younger children may be mainly motivated to create equal outcomes between themselves and others, whereas older children are motivated to enforce fairness norms as a general principle.

Estimates of Prenatal HIV, Hepatitis B Virus, and Hepatitis C Virus Testing Among Pregnant People Enrolled in Wisconsin Medicaid, 2011-2015.

BACKGROUND: The opioid epidemic and rising rates of injection drug use are increasing the risk of human immunodeficiency virus (HIV), hepatitis B virus (HBV), and hepatitis C virus (HCV) infections among pregnant people. According to national clinical guidelines, pregnant people should be universally tested for HIV and HBV, and risk-based tested for HCV. The aim of this study was to determine the proportion and characteristics of prenatal HIV, HBV, and HCV testing and diagnosis among pregnant people with Wisconsin Medicaid coverage between 2011 and 2015. METHODS: Wisconsin birth certificates and Medicaid enrollment data were used to identify the sample. Standard billing and diagnosis codes were used to assess study variables. Data for each pregnancy were analyzed to describe the proportion of pregnancies that had evidence of testing, diagnoses, and yearly trends. RESULTS: Of the 78,917 pregnancies, prenatal testing estimates were 67% for HIV, 73% for HBV, and 6% for HCV. The estimated rate of infections during the study period was 1.82 for HIV, 2.09 for HBV, and 3.52 for HCV per 1000 pregnancies. Compared to the other race/ethnicity groups, pregnant people who were Black were most likely to be tested for HIV (78%) and HBV (80%), and pregnant people who were White were most likely to be tested for HCV (7%). CONCLUSIONS: Clinical testing guidelines have not been effectively translated to practice. Additionally, compared to HIV and HBV, HCV infections during pregnancy are becoming more prevalent, yet current national HCV screening guidelines are the least comprehensive.

Differential use of winter precipitation by upper and lower elevation Douglas fir in the Northern Rockies.

In temperate regions such as the American west, forest trees often exhibit growth sensitivity to climatic conditions of a particular season. For example, annual tree ring growth increments may correlate well with winter precipitation, but not with summer rainfall, suggesting that trees rely more on winter snow than summer rain. Because both the timing and character of seasonal western climate patterns are expected to change considerably over coming decades, variation in the importance of different seasonal moisture sources for trees can be expected to influence how different forest trees respond to climate change as a whole, with shifts in seasonality potentially benefitting some trees while challenging others. In this study, we inferred patterns of tree water use in Douglas fir trees from the Northern Rockies for 2 years using stable water isotopes, while simultaneously quantifying and tracking precipitation inputs to soil moisture across a vertical soil profile. We then coupled water source use with daily measurements of radial growth to demonstrate that soil moisture from winter precipitation accounted for 87.5% and 84% of tree growth at low and high elevations, respectively. We found that prevailing soil moisture conditions drive variation in the depth at which trees access soil water, which in turn determines which seasonal precipitation inputs are available to support tree growth and function. In general, trees at lower elevations relied more on winter precipitation sourced from deep soils while trees at higher elevations made better use of summer rains sourced from near-surface soil layers. As both the timing of seasons and phase of precipitation (rain vs. snow) are likely to change considerably across much of the west, such patterns in tree water use are likely to play a role in determining the evolution of forest composition and structure in a warming climate.

Hydrometeorology organizes intra-annual patterns of tree growth across time, space and species in a montane watershed.

Tree radial growth is often systematically limited by water availability, as is evident in tree ring records. However, the physiological nature of observed tree growth limitation is often uncertain outside of the laboratory. To further explore the physiology of water limitation, we observed intra-annual growth rates of four conifer species using point dendrometers and microcores, and coupled these data to observations of water potential, soil moisture, and vapor pressure deficit over 2 yr in the Northern Rocky Mountains, USA. The onset of growth limitation in four species was well explained by a critical balance between soil moisture supply and atmospheric demand representing relatively mesic conditions, despite the timing of this threshold response varying by up to 2 months across topographic and elevation gradients, growing locations, and study years. Our findings suggest that critical water deficits impeding tissue growth occurred at relatively high water potential values, often occurring when hydrometeorological conditions were relatively wet during the growing season (e.g. in early spring in some cases). This suggests that species-specific differences in water use strategies may not necessarily affect tree growth, and that tissue growth may be more directly linked to environmental moisture conditions than might otherwise be expected.

Increased Risk for Mother-to-Infant Transmission of Hepatitis C Virus Among Medicaid Recipients - Wisconsin, 2011-2015.

State surveillance during the last 10 years reveals a nationwide increase in hepatitis C virus (HCV) infection among young adults (1). The proportion of infants born to HCV-infected women is also increasing nationally (2). To estimate the proportion of infants born to HCV-infected women and the frequency of confirmed HCV infection in their infants, maternal name and date of birth from HCV reports in the Wisconsin Electronic Disease Surveillance System (WEDSS) were linked to Wisconsin Medicaid data for 2011-2015 births. During this period, in the Wisconsin Medicaid population, the proportion of women who had evidence of HCV infection during pregnancy increased 93%, from 1 in 368 pregnancies to 1 in 192. Among 183 infants born to women with evidence of HCV viremia during pregnancy, 34% received recommended HCV testing (3). Mother-to-infant (vertical) transmission was documented in 4% of infants. Improvements in HCV screening practices among pregnant women and infants could enhance identification of infants at risk for vertical transmission of HCV.

Discovery of a Long-Range Perlin Effect in a Conformationally Constrained Oxocane.

Herein, we present the crystal structure, NMR J analysis, and conformational and natural bond order analyses of tricyclic oxocane (1), resulting in the discovery of a long-range Perlin effect at C4 and C5. The normal Perlin effect (NPE) of Δ(1)JC-H = 18.38 Hz at C5 is the largest to date for a nonanomeric methylene due to an unprecedented through-space n → σ* stabilizing interaction. The NPE at C4 where Δ(1)JC-H = 6.91 Hz is nearly double those found in cyclohexanone.

The attentional blink reveals the probabilistic nature of discrete conscious perception.

Attention and awareness are two tightly coupled processes that have been the subject of the same enduring debate: Are they allocated in a discrete or in a graded fashion? Using the attentional blink paradigm and mixture-modeling analysis, we show that awareness arises at central stages of information processing in an all-or-none manner. Manipulating the temporal delay between two targets affected subjects' likelihood of consciously perceiving the second target, but did not affect the precision of its representation. Furthermore, these results held across stimulus categories and paradigms, and they were dependent on attention having been allocated to the first target. The findings distinguish the fundamental contributions of attention and awareness at central stages of visual cognition: Conscious perception emerges in a quantal manner, with attention serving to modulate the probability that representations reach awareness.

A Unified attentional bottleneck in the human brain.

Human information processing is characterized by bottlenecks that constrain throughput. These bottlenecks limit both what we can perceive and what we can act on in multitask settings. Although perceptual and response limitations are often attributed to independent information processing bottlenecks, it has recently been suggested that a common attentional limitation may be responsible for both. To date, however, evidence supporting the existence of such a "unified" bottleneck has been mixed. Here, we tested the unified bottleneck hypothesis using time-resolved fMRI. Experiment 1 isolated brain regions involved in the response selection bottleneck that limits speeded dual-task performance. These same brain regions were not only engaged by a perceptual encoding task in Experiment 2, their activity also tracked delays to a speeded decision-making task caused by concurrent perceptual encoding (Experiment 3). We conclude that a unified attentional bottleneck, including the inferior frontal junction, superior medial frontal cortex, and bilateral insula, temporally limits operations as diverse as perceptual encoding and decision-making.

How well does your e-nose detect cancer? Application of artificial breath analysis for performance assessment.

Comparing electronic nose (e-nose) performance is a challenging task because of a lack of standardised method. This paper proposes a method for defining and quantifying an indicator of the effectiveness of multi-sensor systems in detecting cancers by artificial breath analysis. To build this method, an evaluation of the performances of an array of metal oxide sensors built for use as a lung cancer screening tool was conducted. Breath from 20 healthy volunteers has been sampled in fluorinated ethylene propylene sampling bags. These healthy samples were analysed with and without the addition of nine volatile organic compound (VOC) cancer biomarkers, chosen from literature. The concentration of the VOC added was done in increasing amounts. The more VOC were added, the better the discrimination between 'healthy' samples (breath without additives) and 'cancer' samples (breath with additives) was. By determining at which level of concentration the e-nose fails to reliably discriminate between the two groups, we estimate its ability to well predict the presence of the disease or not in a realistic situation. In this work, a home-made e-nose is put to the test. The results underline that the biomarkers need to be about 5.3 times higher in concentration than in real breath for the home-made nose to tell the difference between groups with a sufficient confidence.

Identification of the Incisive Branch of the Inferior Alveolar Nerve of Edentulous Mandibles Using Cone-Beam Computerized Tomography.

This study explored the average length of the incisive branch (IB) of the inferior alveolar nerve on cone-beam computerized tomography (CBCT) with regard to patient demographics in patients with edentulous mandibles. CBCT was used in a retrospective study of edentulous mandibles to assess the presence and anatomical variation for the IB. Three independent observers measured bilateral IB lengths. In addition to demographics, IB length and port of exit data were obtained. A 1-way analysis of variance was used to test whether IB length varied by sex or port of exit, and a standard Pearson correlation was used to test for IB length and age significance, with a significance level of P < .05. Intraclass correlation coefficients showed significant agreement in IB length across all observers. No significant difference was noted between the exit port and IB length. An important effect was reported for sex, indicating women have generally shorter IB lengths (9.43 ± 3.99 vs 10.55 ± 3.92). There was a significant correlation with age, but the relationship was weak. Edentulous mandibles have an altered anatomic landscape, and establishing predictive IB dimensions aids practitioners in surgical planning.

Perceptual learning: 12-month-olds' discrimination of monkey faces.

Six-month-olds reliably discriminate different monkey and human faces whereas 9-month-olds only discriminate different human faces. It is often falsely assumed that perceptual narrowing reflects a permanent change in perceptual abilities. In 3 experiments, ninety-six 12-month-olds' discrimination of unfamiliar monkey faces was examined. Following 20 s of familiarization, and two 5-s visual-paired comparison test trials, 12-month-olds failed to show discrimination. However, following 40 s of familiarization and two 10-s test trials, 12-month-olds showed reliable discrimination of novel monkey faces. A final experiment was performed demonstrating 12-month-olds' discrimination of the monkey face was due to the increased familiarization rather than increased time of visual comparison. Results are discussed in the context of perceptual narrowing, in particular the flexible nature of perceptual narrowing.

They should have known better: The roles of negligence and outcome in moral judgements of accidental actions.

Two experiments were conducted to investigate the relative influence of agents' negligence and their actions' unintended outcomes on moral judgements. In Study 1, 343 participants were asked in an online questionnaire about a driver whose level of negligence, and the severity of the outcome, were varied systematically. Each judged how much punishment and blame the driver deserved, and rated her negligence, causal responsibility, and intentionality. In Study 2, 341 participants completed the same questionnaire, and also judged the driver's wrongness and the outcome's severity. In both studies, judgements were strongly influenced by negligence; blame was also affected by causal responsibility, and wrongness by intention, but the relatively slight outcome effect on blame and wrongness was largely mediated by negligence. In contrast, both negligence and outcome had substantial effects on punishment judgements; most participants assigned high levels of punishment when, and only when, the outcome was negative and the agent was negligent. These findings shed light on the intriguing phenomenon of moral luck, and indicate that it applies more to punishment judgements than to blame and wrongness. They also indicate that when no negligence information is provided in the description of accidents (as in many previous studies), participants often attribute negligence to agents and judge them accordingly. It seems that the effect of outcome on moral judgements has often been overestimated by researchers, and that of negligence underestimated.

An earlier role for intent in children's partner choice versus punishment.

Among the many factors that influence our moral judgments, two are especially important: whether the person caused a bad outcome and whether they intended for it to happen. Notably, the weight accorded to these factors in adulthood varies by the type of judgment being made. For punishment decisions, intentions and outcomes carry relatively equal weight; for partner choice decisions (i.e., deciding whether or not to interact with someone again), intentions are weighted much more heavily. These behavioral differences in punishment and partner choice judgments may also reflect more fundamental differences in the cognitive processes supporting these decisions. Exploring how punishment and partner choice emerge in development provides important and unique insight into these processes as they emerge and mature. Here, we explore the developmental emergence of punishment and partner choice decisions in 4- to 9-year-old children. Given the importance of intentions for partner choice decisions-from both theoretical and empirical perspectives-we targeted the sensitivity of these two responses to others' intentions as well as outcomes caused. Our punishment results replicate past work: Young children are more focused on outcomes caused and become increasingly sensitive to intentions with age. In contrast, partner choice judgments exhibit sensitivity to intentions at an earlier age than punishment judgments, manifesting as earlier partner choice in cases of attempted violations. These results reveal distinct developmental trajectories for punishment and partner choice judgments, with implications for our understanding of the processes underlying these two responses as well as the development of moral judgment more broadly. (PsycInfo Database Record (c) 2022 APA, all rights reserved).