RESUMO
BACKGROUND/AIM: The transcription factor Y-box-binding protein 1 (YB1) is overexpressed in many types of human cancers. YB1 regulates the G1 phase of the cell cycle by controlling transcription of G1 regulators. Here, we report that YB1 is also involved in regulating G2/M phase. MATERIALS AND METHODS: YB1-depleted TKO cells were subjected to quantitative reverse transcription-polymerase chain reaction and cell-cycle analysis. RNA immunoprecipitation (RIP)-chip assay was performed using anti-YB1 antibodies. Precipitated RNAs were subjected to microarray analysis. RESULTS: Silencing YB1 inhibited the proliferation of TKO cells, which lost the machinery required for G1 phase arrest. Cell-cycle analysis showed that silencing YB1 caused G2/M phase cell-cycle arrest. RIP-chip assay showed that YB1 associated with mRNA of multiple cell-cycle-related genes, including G2/M phase regulators. CONCLUSION: YB1 positively regulates not only the G1 phase but also G2/M phase by regulating multiple cell-cycle-related genes.
Assuntos
Proteínas de Ciclo Celular/metabolismo , Divisão Celular/fisiologia , Neoplasias do Colo/patologia , Fase G2/fisiologia , Mitose/fisiologia , Proteína 1 de Ligação a Y-Box/metabolismo , Animais , Proteínas de Ciclo Celular/genética , Células Cultivadas , Neoplasias do Colo/metabolismo , Embrião de Mamíferos/citologia , Embrião de Mamíferos/metabolismo , Fibroblastos/citologia , Fibroblastos/metabolismo , Humanos , Imunoprecipitação , Camundongos , RNA Mensageiro/genética , RNA Interferente Pequeno/genética , Reação em Cadeia da Polimerase em Tempo Real , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Proteína 1 de Ligação a Y-Box/antagonistas & inibidores , Proteína 1 de Ligação a Y-Box/genéticaRESUMO
We evaluated LaryGard, the cover for the blade of the laryngoscope developed with a purpose of preventing cross infection. By questionnaires we found that there is no adequate guideline for the washing and disinfection of the laryngoscopes at wards in our hospital and the affiliated hospitals. We think that LaryGard is a useful tool in view of preventing the infection and the cost of disinfectants.