RESUMO
A method for the isolation of six isoflavones (genistein, genistin, daidzein, daidzin, glycitein and glycitin) with high purity from Semen sojae praeparatum, a famous traditional Chinese medicine, by preparative HPLC is described.
Assuntos
Medicamentos de Ervas Chinesas/química , Fitoterapia , Plantas Medicinais , Cromatografia Líquida de Alta Pressão , Fermentação , Humanos , Isoflavonas/química , Relação Estrutura-AtividadeRESUMO
AIM: To study the chemical constituents of Marsdenia tenacissima (Roxb.) Wight et Arn. METHODS: To separate compounds with various chromatography technology and to elucidate their structures by chemical and spectral analysis. RESULTS: Two compounds were isolated from Marsdenia tenacissima and their stuctures were determined as tenacissosides J (I) and tenacissosides K (II). CONCLUSION: Compounds I and II are new C21 steroidal glycosides.
Assuntos
Glicosídeos/isolamento & purificação , Marsdenia/química , Plantas Medicinais/química , Esteroides/isolamento & purificação , Glicosídeos/química , Conformação Molecular , Estrutura Molecular , Caules de Planta/química , Esteroides/químicaRESUMO
OBJECTIVE: AIM To study the chemical constituents of the fruit of Eucalyptus globulus. METHOD: Various column chromatographies with silica gel were employed for the isolation and purification. The structures of the compounds were elucidated with of spectral analyses and chemical methods. RESULT: Five compounds were isolated and elucidated as follows: betulonic acid (I), betulinic acid (II), ursolic acid (III), corosolic acid (IV), daucosterol (V). CONCLUSION: Compound I, II, III and IV were isolated from this plant for the first time.