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1.
Cell Tissue Bank ; 13(2): 281-5, 2012 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-21484231

RESUMO

Clinical grade cultivation of human schwann cell by the utilization of human autologous serum instead of fetal bovine serum, and also avoiding any growth factors, can increase safety level of this procedure in cases of clinical cell transplantation. The aim of this study was demonstration of the feasibility of clinical grade schwann cell cultivation. In this experimental study after obtaining consent from close relatives we harvested 10 sural nerves from brain death donors and then cultured in 10 seperated culture media plus autologous serum. We also prepared autologous serum from donor's whole blood. Then cultured cells were evaluated by S100 antibody staining for both morphology and purity. Cell purity range was from 97% to 99% (mean=98.11 ± 0.782%). Mean of the cell count was 14,055.56 ± 2,480.479 per micro liter. There was not significant correlation between cell purity and either the culture period or the age of donors (P>0.05). The spearman correlation coefficient for the cell purity with the period or the age of donors was 0.21 and 0.09, respectively. We demonstrated the feasibility of clinical grade schwann cell cultivation by the using of human autologous serum instead of fetal bovine serum and also without the using of growth factors. We also recommended all cell preparation facilities to adhere to the GMP and other similar quality disciplines especially in the preparation of clinically-used cell products.


Assuntos
Técnicas de Cultura de Células/métodos , Peptídeos e Proteínas de Sinalização Intercelular/farmacologia , Células de Schwann/citologia , Células de Schwann/efeitos dos fármacos , Soro/metabolismo , Adulto , Animais , Bovinos , Células Cultivadas , Demografia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Proteínas S100/metabolismo , Células de Schwann/metabolismo , Coloração e Rotulagem , Nervo Sural/efeitos dos fármacos , Nervo Sural/transplante , Doadores de Tecidos
2.
Acta Med Iran ; 55(6): 368-374, 2017 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-28843237

RESUMO

Stress urinary incontinence is a common medical problem among women. The urethral closure complex and/or the supportive mechanisms are responsible for incontinence in the majority of patients. Several surgical procedures with different degrees of invasiveness and outcomes have been reported to treat the problem. Although most of these procedures are reasonably effective, a general trend towards the study of natural and biocompatible tissues is emerging over popular synthetic materials. Here we report our experience of autologous adipose-derived stem cells transplantation into the periurethral region as a new method of stress urinary incontinence treatment. Ten women with symptoms of stress urinary incontinence were treated by injections of autologous adipose-derived stem cells into the periurethral region via transurethral and transvaginal approach under urethroscopic observation. This report presents the short-term outcome of the patients. The outcome measured by pad test results, ICIQ-SF scores, and Qmax. The mean age of the participants was 45.8±8.7 years. Urinary incontinence significantly decreased through the first two, 6 and 24 weeks after the injection therapy. The difference was significant in pad test results (P<0.001) and ICIQ-SF scores (P<0.001), especially comparing results between 2 and 6 weeks and among 6 and 24 weeks, but not for 2 and 6 weeks compared to each other. Surprisingly, Qmax showed improvement after the study period (means 32.6 vs. 35.7; P=0.002). This study showed that injection of the autologous adipose-derived stem cells to the periurethral region is a safe, yet short-term effective treatment option for stress urinary incontinence. Further studies with longer follow up are needed to confirm its long term efficacy.


Assuntos
Tecido Adiposo/citologia , Transplante de Células-Tronco/métodos , Incontinência Urinária por Estresse/terapia , Adulto , Feminino , Humanos , Injeções , Pessoa de Meia-Idade , Transplante Autólogo , Resultado do Tratamento , Uretra
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