RESUMO
Five new polyoxygenated marine steroids-punicinols A-E (1-5)-were isolated from the gorgonian Leptogorgia punicea and characterized by spectroscopic methods (IR, MS, 1H, 13C and 2-D NMR). The five compounds induced in vitro cytotoxic effects against lung cancer A549 cells, while punicinols A and B were the most active, with IC50 values of 9.7 µM and 9.6 µM, respectively. The synergistic effects of these compounds with paclitaxel, as well as their effects on cell cycle distribution and their performance in the clonogenic assay, were also evaluated. Both compounds demonstrated significant synergistic effects with paclitaxel.
Assuntos
Antozoários/química , Esteroides/química , Esteroides/farmacologia , Animais , Ciclo Celular/efeitos dos fármacos , Linhagem Celular Tumoral , Sinergismo Farmacológico , Humanos , Neoplasias Pulmonares/tratamento farmacológico , Espectroscopia de Ressonância Magnética/métodos , Paclitaxel/farmacologiaRESUMO
The antiherpes effects of the crude extract obtained from Ilex paraguariensis leaves (yerba mate) and their purified fractions were investigated. The most active fraction was selected and assayed to determine the viral multiplication steps upon which it acted. In order to detect the major components of this fraction, thin layer chromatography (TLC) analysis was performed. The antiviral activity was evaluated against HSV-1 and HSV-2 by a viral plaque number reduction assay (IC(50) ) and the cytotoxicity by a MTT assay (CC(50) ). According to the obtained results, all tested samples showed antiherpes activity at noncytotoxic concentrations, and the ethyl acetate fraction was the most active (SI = CC(50) /IC(50) = 188.7 and 264.7 for HSV-1 and HSV-2, respectively). The results also demonstrated that this fraction exerts antiviral activity by the reduction of viral infectivity, the inhibition of virus entry into cells and cell-to-cell virus spread, as well as by the impaired levels of ICP27, ICP4, gD and gE proteins of HSV-1. The TLC analysis showed that this fraction contains monodesmosidic triterpenoid saponins, matesaponin-1 (a bidesmosidic one), caffeic and chlorogenic acids and rutin, which suggests that they could act synergistically and be responsible for the detected antiherpes activity.
Assuntos
Antivirais/farmacologia , Herpesvirus Humano 1/efeitos dos fármacos , Herpesvirus Humano 2/efeitos dos fármacos , Ilex paraguariensis/química , Replicação Viral/efeitos dos fármacos , Acetatos/química , Animais , Antivirais/isolamento & purificação , Sobrevivência Celular , Chlorocebus aethiops , Cromatografia em Camada Fina , Herpesvirus Humano 1/fisiologia , Herpesvirus Humano 2/fisiologia , Proteínas Imediatamente Precoces/metabolismo , Concentração Inibidora 50 , Extratos Vegetais/farmacologia , Folhas de Planta/química , Rutina/isolamento & purificação , Saponinas/isolamento & purificação , Células Vero , Ensaio de Placa ViralRESUMO
This review explores the chemical and biological aspects/results reported in the literature of the octocoral species collected at the Brazilian Coast. This article summarizes the biological activities (including pharmacological, antifouling and others related to chemical ecology) for the compounds and/or extracts described elsewhere. Data and references of compounds isolated from species belonging to the same genus, which have not been investigated in Brazil yet, are presented, emphasizing the importance for research in this area.