RESUMO
AIM: to investigate knowledge, attitude and behaviour toward infection control in two teaching hospitals on the island of Java by means of a questionnaire and to evaluate the use of the questionnaire as a tool. METHODS: we investigated knowledge, attitude and behaviour toward infection control in two teaching hospitals on the island of Java by means of a questionnaire to identify problem areas, barriers and facilitators. The target was to include at least 50% of all health care workers (physicians, nurses, assistant nurses and infection control nurses) in each hospital, department and profession. Differences between demographic variables and scores for individual questions and groups of questions were compared using the chi-square statistic and analysis of variance and Spearman's rho was used to test for correlations between knowledge, attitude, self-reported behaviour and perceived obstacles. RESULTS: more than half of the health care workers of the participating departments completed the questionnaire. Of the 1036 respondents (44% nurses, 37% physicians and 19% assistant nurses), 34% were vaccinated against hepatitis B, 77% had experienced needle stick accidents and 93% had been instructed about infection control. The mean of the correct answers to the knowledge questions was 44%; of the answers to the attitude questions 67% were in agreement with the correct attitude; obstacles to compliance with infection control guidelines were perceived in 30% of the questions and the mean self-reported compliance was 63%. Safe handling of sharps, hand hygiene and the use of personal protective equipment were identified as the most important aspects for interventions. Significant positive correlations were found between knowledge, attitude, self-reported behaviour and perceived obstacles. CONCLUSION: the questionnaire in conjunction with site visits and interviews was a valuable strategy to identify trouble spots in the hospitals and to determine barriers to facilitators of change that should be taken into account when planning interventions. Successful interventions should cover hospital management, the infection control team, as well as the health care workers on the wards.
Assuntos
Atitude do Pessoal de Saúde , Conhecimentos, Atitudes e Prática em Saúde , Pessoal de Saúde/psicologia , Controle de Infecções/normas , Inquéritos e Questionários , Fidelidade a Diretrizes/estatística & dados numéricos , Higiene das Mãos/normas , Pessoal de Saúde/estatística & dados numéricos , Hepatite B/prevenção & controle , Humanos , Indonésia , Ferimentos Penetrantes Produzidos por Agulha/prevenção & controle , Guias de Prática Clínica como Assunto , Equipamentos de Proteção , Vacinação/estatística & dados numéricosRESUMO
Large Q-fever outbreaks were reported in The Netherlands from May 2007 to 2009, with dairy-goat farms as the putative source. Since Q-fever outbreaks at such farms were first reported in 2005, we explored whether there was evidence of human outbreaks before May 2007. Space-time scan statistics were used to look for clusters of lower-respiratory infections (LRIs), hepatitis, and/or endocarditis in hospitalizations, 2005-2007. We assessed whether these were plausibly caused by Q fever, using patients' age, discharge diagnoses, indications for other causes, and overlap with reported Q fever in goats/humans. For seven detected LRI clusters and one hepatitis cluster, we considered Q fever a plausible cause. One of these clusters reflected the recognized May 2007 outbreak. Real-time syndromic surveillance would have detected four of the other clusters in 2007, one in 2006 and two in 2005, which might have resulted in detection of Q-fever outbreaks up to 2 years earlier.
Assuntos
Doenças das Cabras/epidemiologia , Febre Q/veterinária , Adolescente , Adulto , Idoso , Animais , Criança , Pré-Escolar , Análise por Conglomerados , Doenças das Cabras/microbiologia , Doenças das Cabras/transmissão , Cabras , Hospitais , Humanos , Lactente , Recém-Nascido , Pessoa de Meia-Idade , Países Baixos/epidemiologia , Vigilância da População , Febre Q/epidemiologia , Febre Q/transmissão , Estudos Retrospectivos , Fatores de Tempo , Adulto Jovem , ZoonosesRESUMO
This study documents the determinants and plasma levels of N-terminal pro-brain natriuretic peptide (NT-proBNP) among hypertensive and normotensive subjects in a multi-ethnic population in the United Arab Emirates (UAE). We obtained demographic, anthropometric and clinical data, together with fasting NT-proBNP and biochemical indices from 128 hypertensive patients and 138 normotensive subjects matched for age, gender and ethnicity. Plasma NT-proBNP levels were significantly (P<0.001), and several-fold higher among hypertensives (median 5.92, inter quartile range (IQR): 1.79-18.48 pmol/l) than normotensives (median 1.78, IQR: 0.59-4.32 pmol/l) in the total study population, and the same was true for the ethnic groups separately. Similarly, plasma levels of glucose, blood urea nitrogen (BUN) and creatinine, but not insulin, were significantly (P<0.05) higher among hypertensives than normotensives. For all subjects combined, log NT-proBNP correlated positively and significantly with age (P<0.01), log glucose (P<0.05), systolic blood pressure (SBP, P<0.001), log BUN (P<0.001) and log creatinine (P<0.001). Multivariate regression analysis showed that NT-proBNP levels were independently and positively correlated with SBP, age, gender, log BUN, Emirati and South East Asian ethnic groups and inversely associated with current exercise. In conclusion, we found circulating levels of NT-proBNP to be significantly increased in hypertensive versus normotensive subjects in the UAE and independently related to SBP, age, gender, indices of renal function and possibly exercise. Our results further suggest a possible modulating effect of ethnicity on NT-proBNP levels.
Assuntos
Hipertensão/sangue , Peptídeo Natriurético Encefálico/sangue , Fragmentos de Peptídeos/sangue , Adulto , Exercício Físico , Feminino , Humanos , Hipertensão/etnologia , Masculino , Pessoa de Meia-Idade , Emirados Árabes Unidos/etnologiaRESUMO
Standard precautions can prevent transmission of micro-organisms. This study investigated hand hygiene, handling of needles and use of personal protective equipment in an Indonesian teaching hospital, and performed a multi-faceted intervention study to improve compliance. An intervention was performed in an internal medicine ward and a paediatric ward, consisting of development of a protocol for standard precautions, installation of washstands, educational activities and performance feedback. Before, during and after the intervention, observers monitored compliance with hand hygiene, safe handling of needles and use of gloves, gowns and masks. A gynaecology ward served as the control. Unobtrusive observations were performed to check for an influence of the observers on the overt observations. In total, 7,160 activities were observed. Compliance with hand hygiene increased from 46% to 77% in the internal medicine ward and from 22% to 62% in the paediatric ward. Before the intervention, no safe recapping of needles was recorded in either ward. After the intervention, 20% of needles were recapped safely. Inappropriate gown use decreased in the internal medicine ward. There were no significant changes in use of gloves and masks. There may have been an effect of the overt observations in the paediatric ward, but there was no effect in the internal medicine ward. There were no significant changes in the control ward, except for a decrease in the use of gloves. In conclusion, compliance with hand hygiene procedures improved significantly due to an intervention project focused on education and improved facilities. Compliance with safe handling of needles improved slightly due to introduction of the one-handed method for safe recapping of used needles.
Assuntos
Infecção Hospitalar/prevenção & controle , Guias como Assunto/normas , Controle de Infecções , Precauções Universais/métodos , Desinfecção das Mãos/métodos , Desinfecção das Mãos/normas , Pessoal de Saúde/educação , Hospitais de Ensino , Humanos , Indonésia , Controle de Infecções/métodos , Controle de Infecções/normas , Ferimentos Penetrantes Produzidos por Agulha/prevenção & controleRESUMO
A cross-sectional surveillance of healthcare-associated infections (HAIs) and exposure to risk factors was undertaken in two Indonesian teaching hospitals (Hospitals A and B). Patients from internal medicine, surgery, obstetrics and gynaecology, paediatrics, a class department and intensive care were included. Patient demographics, antibiotic use, culture results, presence of HAI [phlebitis, surgical site infection (SSI), urinary tract infection (UTI) and septicaemia] and risk factors were recorded. To check for interobserver variation, a validation study was performed in Hospital B. In Hospitals A and B, 1,334 and 888 patients were included, respectively. Exposure to invasive devices and surgery was 59%. In Hospital A, 2.8% of all patients had phlebitis, 1.7% had SSI, 0.9% had UTI and 0.8% had septicaemia. In Hospital B, 3.8% had phlebitis, 1.8% had SSI, 1.1% had UTI and 0.8% had septicaemia. In the validation study, the prevalence as recorded by the first team was 2.6% phlebitis, 1.8% SSI, 0.9% UTI and no septicaemia, and that recorded by the second team was 2.2% phlebitis, 2.6% SSI, 3.5% UTI and 0.9% septicaemia. This study is the first to report on HAI in Indonesia. Prevalence rates are comparable to those in other countries. The reliability of the surveillance was insufficient as a considerable difference in prevalence rates was found in the validation study. The surveillance method used is a feasible tool for hospitals in countries with limited healthcare resources to estimate their level of HAI and make improvements in infection control. Efficiency can be improved by restricting the surveillance to include only those patients with invasive procedures. This can help to detect 90% of all infections while screening only 60% of patients.
Assuntos
Infecção Hospitalar/epidemiologia , Pesquisas sobre Atenção à Saúde , Hospitais de Ensino , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Criança , Pré-Escolar , Infecção Hospitalar/etiologia , Estudos Transversais , Feminino , Humanos , Indonésia/epidemiologia , Lactente , Recém-Nascido , Controle de Infecções , Masculino , Pessoa de Meia-Idade , Variações Dependentes do Observador , Flebite/epidemiologia , Prevalência , Sepse/epidemiologia , Infecções Urinárias/epidemiologiaRESUMO
We aimed to explore the level of inter- and intra-individual variation in applied force when listening at a surface, and assess the resulting variation in earprints. We further intended to identify possible sources of this variation. Forty subjects each listened twenty-four times at a surface while applied force was recorded. In between efforts the level and frequency of the target sound, and the level of ambient noise were varied. Each listening effort was characterized by two values: the mean of a series of force recordings ('functional force') and the highest force reading of the effort ('peak value'). A mixed model analysis of variance revealed that repetition during multiple efforts of listening and the level of the target sound significantly affected both values for applied force. The frequency of the target sound affected the peak value, but we assume this was due to confounding effects. The level of ambient noise did not affect applied force. To explore the correlation between values for applied force of various efforts by single ear, the intra-class correlation coefficient was calculated. For functional force it was 0.80; for the peak value it was 0.79. To study intra-individual variation in earprints, five prints from each ear were lifted and studied. Variation in prints is discussed.
Assuntos
Orelha Externa/anatomia & histologia , Medicina Legal/métodos , Análise de Variância , Fenômenos Biomecânicos , Feminino , Humanos , Masculino , PressãoRESUMO
OBJECTIVE: 1) To assess the regression to normal cytology in women with cervical smears diagnosed as atypical squamous or glandular cells of undetermined significance (ASCUS/AGUS) and absence or clearance of human papillomavirus (HPV) infection; 2) To evaluate the association between viral load, semi-quantitatively evaluated, and cytological or histological outcome. MATERIAL AND METHODS: In this cohort study HPV test and biopsy was taken in 148 women with ASCUS/AGUS cytology. After 12-18 months a HPV test and cervical smear were repeated in 121 women. RESULTS: Absence or clearance of HPV showed significantly more regression to normal cytology than persistent or newly acquired infected women, odds ratio 27 (95% confidence interval; 7-103). The viral load of the HPV test at enrollment was not correlated with the follow-up cytological outcome (Spearman correlation coefficient 0.2, p = 0.2). A marked association between viral load and histological outcome at enrollment was shown (Spearman correlation coefficient 0.43, p < 0.0001). CONCLUSION: Absence or clearance of HPV can predict regression to normal cytology. Viral load at enrollment cannot predict cytological regression. There was a marked association between viral load and the underlying CIN at enrollment. However, there was large overlapping of viral loads among the grades of CIN. Therefore, viral load is not a useful parameter to predict high-grade lesions in women with ASCUS/AGUS cytology.
Assuntos
Papillomaviridae , Infecções por Papillomavirus/complicações , Displasia do Colo do Útero/patologia , Displasia do Colo do Útero/virologia , Carga Viral , Adolescente , Adulto , Idoso , Estudos de Coortes , Feminino , Humanos , Pessoa de Meia-Idade , Valor Preditivo dos Testes , Remissão Espontânea , Resultado do TratamentoRESUMO
Occasionally ear prints are found at crime scenes. The height of the ear imprint may provide the police with information regarding the stature of the perpetrator and may therefore help to narrow down the number of suspects. The research provides calculations for the determination of stature from the height of the tragus imprint found at crime scenes. It takes into account various variables such as age, stature and gender.
Assuntos
Estatura , Orelha , Ciências Forenses/métodos , Adulto , Distribuição por Idade , Feminino , Humanos , Modelos Lineares , Masculino , Pessoa de Meia-Idade , Valores de Referência , Fatores SexuaisRESUMO
The purpose of this research was to model the familial clustering of breast cancer and to provide an accurate risk estimate for individuals from the general population, based on their family history of breast and ovarian cancer. We constructed a genetic model as an extension of a model by Claus et al. (E. B. Claus et al., Am. J. Hum. Genet., 48: 232-242, 1991), with three breast cancer genes, BRCA1, BRCA2, and a hypothetical BRCAu, in two variants, one in which BRCAu was dominant and one in which BRCAu was recessive. The model parameters were estimated using published estimates of population incidence and relative risks. Risk estimation was performed for a set of 196 counselees and for a set of simulated counselees with both the dominant BRCAu and the recessive BRCAu model, and compared relating to medical management. Estimates of the model parameters were found. Relative risks among family members were comparable between the model of Claus et al. (E. B. Claus et al., Am. J. Hum. Genet., 48: 232-242, 1991) and our model. The dominant and the recessive model provided approximately similar lifetime risks for breast cancer. Our model is suitable for breast cancer risk estimation in a health care setting.
Assuntos
Neoplasias da Mama/epidemiologia , Neoplasias da Mama/genética , Genes BRCA1 , Genes BRCA2 , Predisposição Genética para Doença/epidemiologia , Adulto , Distribuição por Idade , Idoso , Análise por Conglomerados , Feminino , Testes Genéticos , Heterozigoto , Humanos , Incidência , Pessoa de Meia-Idade , Modelos Genéticos , Países Baixos/epidemiologia , Linhagem , Prognóstico , Medição de RiscoRESUMO
OBJECTIVE: To determine whether elimination of tuberculosis in the Dutch population can be achieved by the year 2030, taking into account the impact of immigration. METHODS: The incidence of tuberculosis (all forms) in the period 1970 to 2030 was estimated using a life-table model for the Dutch population without the impact of immigration. The influence of immigration on tuberculosis incidence among the Dutch was modelled using four immigrant scenarios, distinguished by the assumed contact rate between immigrants and the Dutch population, and by different projections (middle, upper) of the future size of the immigrant population in The Netherlands. RESULTS: The incidence of smear-positive tuberculosis among the Dutch is projected to be 1.4 per million in the scenario without the influence of immigrant cases, and ranging from 3.8 to 11.8 per million in the four immigrant scenarios. In all immigrant scenarios, the prevalence of tuberculosis infection will continue to decline and be less than 1% by the year 2030. At least 60% of Dutch tuberculosis cases in the year 2030 are expected to be the result of transmission from a foreign source case. CONCLUSION: Using a prevalence of tuberculosis infection of less than 1% as the elimination criterion, tuberculosis will probably be eliminated from the indigenous Dutch population by 2030. However, the incidence of smear-positive tuberculosis is expected to remain higher than 1 per million, and the majority of new tuberculosis cases among the Dutch may be attributable to recent infection from a foreign source case.
Assuntos
Emigração e Imigração/estatística & dados numéricos , Tuberculose Pulmonar/epidemiologia , Adolescente , Adulto , Distribuição por Idade , Idoso , Criança , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Modelos Estatísticos , Países Baixos/epidemiologia , Prevalência , Fatores de Risco , Distribuição por Sexo , Tuberculose Pulmonar/prevenção & controleRESUMO
AIMS: To assess the prevalence of general health status, use of sleep medication, and use of medication for cardiovascular diseases, and to study their relation to aircraft noise exposure. METHODS: These health indicators were measured by a cross-sectional survey among 11 812 respondents living within a radius of 25 km around Schiphol airport (Amsterdam). RESULTS: Adjusted odds ratios ranged from 1.02 to 2.34 per 10 dB(A) increase in L(den). The associations were statistically significant for all indicators, except for use of prescribed sleep medication or sedatives and frequent use of this medication. None of the health indicators were associated with aircraft noise exposure during the night, but use of non-prescribed sleep medication or sedatives was associated with aircraft noise exposure during the late evening (OR = 1.72). Vitality related health complaints such as tiredness and headache were associated with aircraft noise, whereas most other physical complaints were not. Odds ratios for the vitality related complaints ranged from 1.16 to 1.47 per 10 dB(A) increase in L(den). A small fraction of the prevalence of poor self rated health (0.13), medication for cardiovascular diseases or increased blood pressure (0.08), and sleep medication or sedatives (0.22) could be attributed to aircraft noise. Although the attributable fraction was highest in the governmentally noise regulated area, aircraft noise had more impact in the non-regulated area, due to the larger population. CONCLUSIONS: Results suggest associations between community exposure to aircraft noise and the health indicators poor general health status, use of sleep medication, and use of medication for cardiovascular diseases.
Assuntos
Aeronaves , Doenças Cardiovasculares/etiologia , Exposição Ambiental/efeitos adversos , Ruído dos Transportes/efeitos adversos , Transtornos do Sono-Vigília/etiologia , Adolescente , Adulto , Idoso , Doenças Cardiovasculares/tratamento farmacológico , Estudos Transversais , Feminino , Nível de Saúde , Humanos , Masculino , Pessoa de Meia-Idade , Países Baixos , Razão de Chances , Medição de Risco , Sensibilidade e EspecificidadeRESUMO
Most parasites have complex life cycles; and mathematical models can help in targeting interventions and predicting disease-control efforts. For actual applications, quantification and validation of models is a key issue. We illustrate the process of validation by presenting a (re)analysis of fly-feeding experiments carried out by the Onchocerciasis (river blindness) Control Programme (OCP/WHO) in West Africa, with the objective to validate ONCHOSIM, an onchocerciasis transmission model. In these experiments flies were fed on human patients and dissected to count the number of microfilariae they had ingested. To assess microfilarial skin densities, skin snips (biopsies) were taken and examined. Originally, the resulting curve was interpreted as showing saturation and considered the main regulating (density-dependent) mechanism of onchocerciasis transmission in the model. Taking into account measurement errors in the skin microfilarial density of human subjects (on whom the flies were fed) we now conclude that the relationship is essentially linear. This prompts us to requantify ONCHOSIM. Possible alternative density-dependent mechanisms are discussed.
Assuntos
Oncocercose/transmissão , Adulto , Animais , Feminino , Humanos , Masculino , Modelos Teóricos , Oncocercose/epidemiologia , Simuliidae , Pele/parasitologiaRESUMO
The campylobacter status of 495 broiler flocks sampled in The Netherlands between April 1997 and December 2000 was related to farm- and flock-specific information obtained from questionnaires to identify potential risk factors for campylobacter presence. Approximately 30% of the flocks tested positive for Campylobacter spp. in at least one pooled faecal sample. Multivariable logistic regression showed significant risk increments for: ages 29-35 days (OR = 2.34) and 36-42 days (OR = 3.96) compared to 22-28 days; > or =5 broiler houses on the premises (OR = 3.02); the presence of other farm animals on the farm (OR = 1.88); the presence of animals on farms within 1 km (OR = 9.56); and summer (OR = 3.48) and fall (OR = 2.59) compared to winter. Furthermore, hatcheries differed (ORs from 5.42 to 20.2), although this variable showed strong collinearity with the variables explaining feed mill and integrated poultry operations. We also identified interaction in which an increased risk from children entering the broiler house (OR = 28.0) was diminished by the use of broiler-specific workclothes. Population attributable fractions (PAFs) suggested that animals on farms within 1 km (PAF = 0.76) and hatchery (PAF = 0.67) had the highest impacts on campylobacter presence in Dutch broiler flocks. These factors, however, lack an easy interpretation of the mechanism behind the suggested effect.
Assuntos
Criação de Animais Domésticos/métodos , Infecções por Campylobacter/veterinária , Campylobacter/isolamento & purificação , Galinhas , Doenças das Aves Domésticas/epidemiologia , Fatores Etários , Análise de Variância , Animais , Infecções por Campylobacter/epidemiologia , Infecções por Campylobacter/etiologia , Estudos Transversais , Fezes/microbiologia , Feminino , Modelos Logísticos , Masculino , Análise Multivariada , Países Baixos/epidemiologia , Densidade Demográfica , Doenças das Aves Domésticas/etiologia , Roupa de Proteção , Fatores de Risco , Estações do Ano , Inquéritos e QuestionáriosAssuntos
Neoplasias da Mama/epidemiologia , Neoplasias da Mama/genética , Atenção Primária à Saúde/estatística & dados numéricos , Adulto , Fatores Etários , Proteína BRCA1/genética , Proteína BRCA2/genética , Saúde da Família , Feminino , Frequência do Gene , Humanos , Incidência , Programas de Rastreamento/métodos , Pessoa de Meia-Idade , Modelos Genéticos , Mutação , Países Baixos/epidemiologia , Penetrância , PrevalênciaRESUMO
The aim of this study was to describe a systematic process of record-linkage, cross-validation, case-ascertainment and capture-recapture analysis to assess the quality of tuberculosis registers and to estimate the completeness of notification of incident tuberculosis cases in The Netherlands in 1998. After record-linkage and cross-validation 1499 tuberculosis patients were identified, of whom 1298 were notified, resulting in an observed under-notification of 13.4%. After adjustment for possible imperfect record-linkage and remaining false-positive hospital cases observed under-notification was 7.3%. Log-linear capture-recapture analysis initially estimated a total number of 2053 (95% CI 1871-2443) tuberculosis cases, resulting in an estimated under-notification of 36.8%. After adjustment for possible imperfect record-linkage and remaining false-positive hospital cases various capture-recapture models estimated under-notification at 13.6%. One of the reasons for the higher than expected estimated under-notification in a country with a well-organized system of tuberculosis control might be that some tuberculosis cases, e.g. extrapulmonary tuberculosis, are managed by clinicians less familiar with notification of infectious diseases. This study demonstrates the possible impact of violation of assumptions underlying capture-recapture analysis, especially the perfect record-linkage, perfect positive predictive value and absent three-way interaction assumptions.
Assuntos
Sistema de Registros , Tuberculose/epidemiologia , Notificação de Doenças , Métodos Epidemiológicos , Humanos , Países Baixos/epidemiologiaRESUMO
The prevalence of childhood atopic disorders has risen dramatically in the last decades of the past century. Risk factors for the development of these disorders have been studied extensively. This review focuses on the role of early life risk factors such as pre-natal development, perinatal circumstances, birth order and childhood vaccinations.
Assuntos
Hipersensibilidade Imediata/etiologia , Ordem de Nascimento , Criança , Pré-Escolar , Feminino , Desenvolvimento Fetal , Humanos , Hipersensibilidade Imediata/epidemiologia , Lactente , Gravidez , Efeitos Tardios da Exposição Pré-Natal , Fatores de Risco , VacinaçãoRESUMO
The distribution of antibody levels to Legionella (L.) pneumophila (serotypes 1-7) was compared between subjects who worked near the source of a large outbreak of Legionnaires' disease (n=668) and a population sample of comparable age (n=480). In a previous analysis of these data, it was estimated that 80% of those working near the source were infected with L. pneumophila. However, the estimation procedure implicitly assumes that the probability of infection does not depend on the antibody level of a person before exposure. This is questionable, as antibodies could protect against infection. We have now estimated the minimum value consistent with the data on the number of infected persons. We observed that a minimum of 40% [95% confidence interval (CI) 32-48] of those working near the source and 13% (95% CI 8-18) of those working further away were infected with L. pneumophila. Implications of these findings for design options in future research are discussed.
Assuntos
Surtos de Doenças , Doença dos Legionários/epidemiologia , Adolescente , Adulto , Idoso , Anticorpos Antibacterianos/sangue , Humanos , Imunoglobulina G/sangue , Imunoglobulina M/sangue , Pessoa de Meia-Idade , Exposição OcupacionalRESUMO
The ability of national reference laboratories for Salmonella of the European Union member states to detect Salmonella bacteria was tested in four collaborative studies during the period 1995 through 1999. Three different methods were prescribed in the four studies. Capsules containing various numbers of Salmonella Typhimurium or Salmonella Enteritidis were tested. In studies II, III and IV, Salmonella bacteria were isolated in the presence of competitive microorganisms. Significant differences were found between the four studies due to varying levels of difficulty with regard to the level of contamination, the use of serotypes and the presence of competitive organisms. There were also significant differences between the laboratories in the results obtained. Possible reasons for these differences will be further investigated by the European Union Community Reference Laboratory for Salmonella.
Assuntos
União Europeia , Laboratórios/normas , Salmonella enteritidis/isolamento & purificação , Salmonella typhimurium/isolamento & purificação , Técnicas de Tipagem Bacteriana , Técnicas Bacteriológicas/normas , Cápsulas , Meios de Cultura , Humanos , Variações Dependentes do Observador , Padrões de Referência , Salmonella enteritidis/classificação , Salmonella typhimurium/classificaçãoRESUMO
The capacity of national reference laboratories of the European Union member states to correctly serotype Salmonella strains was assessed in four collaborative studies on serotyping in the period 1995-1999. Participants were asked to identify 20 strains in studies I, II and III and 16 strains in study IV, using the typing method routinely performed in their laboratory. In the first study, the strains to be identified belonged to Salmonella enterica subsp. enterica, salamae or houtenae, while in the other studies only strains belonging to Salmonella enterica subsp. enterica were included. Significant differences between laboratories and between studies were found. Differences were related to the frequency of actual occurrence of the study strains in the area served by the laboratory and the number of antisera available in the laboratory.
Assuntos
Técnicas de Tipagem Bacteriana/métodos , Técnicas de Tipagem Bacteriana/normas , Salmonella/classificação , Salmonella/isolamento & purificação , União Europeia , Padrões de ReferênciaRESUMO
The standard assay for onchocerciasis diagnosis is microscopical detection of microfilariae in skin snips. Skin snipping is painful, requires appropriate sterilization of equipment, and may fail to diagnose light infections. Two alternatives are a polymerase chain reaction (PCR) test which detects parasite DNA in pieces or scrapings of skin and a test based on allergic reactions to topical application of diethylcarbamazine (DEC). We compared these 2 diagnostics with standard skin snip microscopy in 313 individuals from 2 villages in Guinea, with low prevalence after over 10 years of control by the Onchocerciasis Control Programme. Lower and upper bounds on sensitivities and specificities of these 3 tests were estimated. In addition, these parameters were estimated using 5 different statistical models. Where prevalence was low, PCR and the DEC patch test appeared to be more sensitive than skin snipping which has low sensitivity. As the DEC test is non-invasive, simple and cheap, it may provide a good alternative to skin snipping alone for surveillance in low prevalence areas.