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Biochem Biophys Res Commun ; 491(4): 980-985, 2017 09 30.
Artigo em Inglês | MEDLINE | ID: mdl-28765046

RESUMO

S100A6 is a Ca2+-signal transducer that interacts with numerous proteins and regulates their biochemical functions. Here we identified a centrosomal protein, FOR20 (FOP-related protein of 20 kDa) as a novel S100A6 target by screening protein microarrays carrying 19,676 recombinant GST-fused human proteins. Binding experiments revealed that S100A6 interacts with the N-terminal region (residues 1-30) of FOR20 in a Ca2+-dependent manner in vitro and in living cells. Several S100 proteins including S100A1, A2, A4, A11, B also exhibited Ca2+-dependent interactions with FOR20 as well as S100A6. We found that two distantly related centrosomal proteins, FOP and OFD1, also possess N-terminal regions with a significant sequence similarity to the putative S100A6-binding site (residues 1-30) in FOR20 and are capable of binding to S100A6 in a Ca2+-dependent manner. Taken together, these results may indicate that S100A6 interacts with FOR20 and related centrosomal proteins through a conserved N-terminal domain, suggesting a novel Ca2+-dependent regulation of centrosomal function.


Assuntos
Proteínas de Ciclo Celular/química , Proteínas de Ciclo Celular/metabolismo , Proteínas/química , Proteínas/metabolismo , Proteínas S100/química , Proteínas S100/metabolismo , Animais , Células COS , Células Cultivadas , Centrossomo/química , Centrossomo/metabolismo , Chlorocebus aethiops , Células HeLa , Humanos , Análise Serial de Proteínas , Ligação Proteica , Proteína A6 Ligante de Cálcio S100 , Especificidade por Substrato
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