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Introduction,: Graft-versus-host disease (GVHD) is a recognized complication of allogeneic stem cell transplantation (allo-SCT) and may affect muscle. We investigated the incidence and subtypes of inflammatory myopathy (IM) in South Australian recipients of allo-SCT. METHODS: Recipients of allo-SCT from 2004 to 2014 at the Royal Adelaide Hospital were identified. Records were reviewed to identify patients with weakness, creatine kinase (CK) elevation, and muscle biopsy confirming IM. RESULTS: Weakness was present in 32 of 224 patients who received allo-SCT patients reviewed, and CK was raised in 7 of 20 patients with weakness. Six patients developed biopsy-confirmed IM; 3 patients had chronic GVHD-related myopathy, 2 had necrotizing myopathy, and 1 had dermatomyositis (DM) associated with anti-melanoma differentiation associated protein 5 (MDA5) antibodies. The incidence of IM was calculated to be 2 cases per thousand annually. DISCUSSION: Among recipients of allo-SCT, weakness is common, and the incidence of IM is increased. Histopathological diagnoses are varied, and we report findings of necrotizing myopathy and anti-MDA5-associated DM. Muscle Nerve 58:790-795, 2018.
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Doença Enxerto-Hospedeiro/etiologia , Miosite/etiologia , Complicações Pós-Operatórias/etiologia , Transplante de Células-Tronco/efeitos adversos , Adolescente , Adulto , Idoso , Aloenxertos/metabolismo , Antígenos CD/metabolismo , Austrália , Creatina Quinase/sangue , Eletromiografia , Feminino , Antígenos de Histocompatibilidade/metabolismo , Humanos , Incidência , Masculino , Pessoa de Meia-Idade , Debilidade Muscular/etiologia , Miosite/epidemiologia , Prevalência , Estudos Retrospectivos , Adulto JovemRESUMO
INTRODUCTION: The role of vaccinations and infections in triggering idiopathic inflammatory myopathies (IIM) has not been confirmed. METHODS: Among patients with histologically confirmed myositis, infections or vaccinations administered prior to myositis onset were determined. The characteristics of this group were compared with controls (myositis patients without prior infection or vaccination). RESULTS: The frequency of IIM with a prior vaccination was 20 of 206 (9.7%), infection was 29 of 206 (14%), and either vaccination or infection was 49 of 206 (23.8%). Dermatomyositis (DM) was more frequent among patients with preceding vaccination (P = 0.03) or prior infections (P = 0.02) than among controls. Antibodies to Ro52 were more frequent among patients with preceding vaccination than among controls (P = 0.002). DISCUSSION: Although causality is not shown, the occurrence of prior infection or vaccination in 24% of patients with IIM prompts further inquiry. The overrepresentation of DM in those with preceding vaccination and the possible role of antibodies to Ro52 in susceptibility to vaccine-induced myositis require confirmation. Muscle Nerve 56: 987-989, 2017.
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Infecções/etiologia , Miosite/epidemiologia , Miosite/etiologia , Vacinação/efeitos adversos , Adulto , Austrália/epidemiologia , Autoanticorpos/sangue , Feminino , Humanos , Masculino , Ribonucleoproteínas/imunologiaRESUMO
RATIONALE: It is unclear how septic shock causes acute kidney injury (AKI) and whether this is associated with histological change. OBJECTIVES: We aimed to determine the nature and extent of changes in renal structure and function over time in an ovine model of septic shock. METHODS: Fifteen sheep were instrumented with a renal artery flow probe and renal vein cannula. Ten were given intravenous Escherichia coli to induce septic shock, and five acted as controls. Animals were mechanically ventilated for 48 hours, while receiving protocol-guided parenteral fluids and a norepinephrine infusion to maintain mean arterial pressure. Renal biopsies were taken every 24 hours or whenever animals were oliguric for 2 hours. A renal pathologist, blinded to tissue source, systematically quantified histological appearance by light and electron microscopy for 31 prespecified structural changes. MEASUREMENTS AND MAIN RESULTS: Sheep given E. coli developed septic shock, oliguria, increased serum creatinine, and reduced creatinine clearance (AKI), but there were no changes over time in renal blood flow between groups (P > 0.30) or over time within groups (P > 0.50). Renal oxygen consumption increased only in nonseptic animals (P = 0.01), but there was no between-group difference in renal lactate flux (P > 0.50). There was little structural disturbance in all biopsies and, although some cellular appearances changed over time, the only difference between septic and nonseptic animals was mesangial expansion on electron microscopy. CONCLUSIONS: In an intensive care-supported model of gram-negative septic shock, early AKI was not associated with changes in renal blood flow, oxygen delivery, or histological appearance. Other mechanisms must contribute to septic AKI.
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Rim/fisiopatologia , Choque Séptico/fisiopatologia , Injúria Renal Aguda/etiologia , Animais , Biópsia , Pressão Sanguínea , Débito Cardíaco , Modelos Animais de Doenças , Feminino , Rim/patologia , Circulação Renal , Ovinos , Choque Séptico/complicações , Choque Séptico/patologiaRESUMO
Adenoviruses are common pathogens that have the potential to cause opportunistic infections with significant morbidity and mortality in immunocompromised hosts. The significance of adenoviral infection and disease is incompletely known in the setting of kidney transplantation. Reported adenovirus infections in renal transplant recipients have typically manifested as haemorrhagic cystitis and tubulointerstitial nephritis. Pneumonia, hepatitis and enteritis are often seen in other solid organ recipients. However, disseminated or severe adenovirus infections, including fatal cases, have been described in renal transplant recipients. There is uncertainty regarding monitoring and treatment of this virus. Although not supported by randomized clinical trials, cidofovir is used for the treatment of adenovirus disease not responding to reduction of immunosuppression. We present a case series of 2 patients with disseminated adenovirus infection in our centre who presented at different times from the time of transplantation.
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Infecções por Adenoviridae , Transplante de Rim , Complicações Pós-Operatórias/virologia , Infecções por Adenoviridae/tratamento farmacológico , Idoso , Feminino , Humanos , Pessoa de Meia-Idade , Complicações Pós-Operatórias/tratamento farmacológicoAssuntos
Adalimumab/efeitos adversos , Antirreumáticos/efeitos adversos , Artrite Psoriásica/tratamento farmacológico , Linfócitos B/patologia , Doenças Orbitárias/induzido quimicamente , Pseudolinfoma/induzido quimicamente , Idoso , Linfócitos B/efeitos da radiação , Humanos , Masculino , Doenças Orbitárias/diagnóstico por imagem , Doenças Orbitárias/radioterapia , Pseudolinfoma/diagnóstico por imagem , Pseudolinfoma/radioterapia , Radioterapia , Tomografia Computadorizada por Raios XRESUMO
The discovery of almost invariable mouse double minute 2 (MDM2) amplification among atypical lipomatous tumors (ALT)/well-differentiated liposarcoma and dedifferentiated liposarcoma is incorporated into the contemporary diagnostic workup of fatty lesions. MDM2 amplifications are also found frequently in intimal sarcomas and in low-grade osteogenic sarcoma. At present, fluorescence in situ hybridization (FISH) is the reference test for MDM2 assessment. We are interested in evaluating silver in situ hybridization (SISH) for this purpose. Between October 2016 and May 2020, in 192 consecutive cases requiring MDM2 FISH, SISH was also performed concurrently, including 77 (40.1%) core biopsies and 115 (58.9%) surgical specimens. The mean patient age was 61.0 years. SISH results were available overnight or within 48 hours if repeat testing was required. FISH results were available within 2 to 5 weeks. The cost of SISH was one third of FISH. FISH demonstrated MDM2 amplification in 44 cases (23.6%), was negative in 144 cases (74.4%) and nondiagnostic in 4 decalcified cases (2.0%). SISH showed MDM2 amplification in 33 cases (17.2%), no amplification in 119 cases (62.0%), and indeterminate results because of poor signal in 40 (20.8%) cases. All 33 (100%) SISH-amplified tumors and 113 of 119 (95.0%) nonamplified results were confirmed by FISH. There were no clear differences in the performance of SISH on NCB versus surgical specimens. The overall performance indices of SISH are sensitivity 75%, specificity 78.5%, positive predictive value 100%, and negative predictive value 95.8%. FISH is not required when SISH is clearly amplified. This is clinically useful and improves efficiency. Nonamplified SISH results provide early indications of the likely FISH findings, but there is a 4.2% chance of FISH being positive. At present, the main drawback of SISH is the high rate of nondiagnostic tests. Optimization of SISH signal detection to reduce the proportion of indeterminate results is our current focus.
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Neoplasias Ósseas , Lipoma , Lipossarcoma , Sarcoma , Animais , Camundongos , Amplificação de Genes , Prata , Hibridização in Situ Fluorescente/métodos , Lipoma/genética , Proteínas Proto-Oncogênicas c-mdm2/genética , Proteínas Proto-Oncogênicas c-mdm2/metabolismo , Lipossarcoma/diagnósticoRESUMO
Pathogenic variants in DNMT3A are most commonly associated with Tatton-Brown-Rahman Syndrome (TBRS), but includes other phenotypes such as Heyn-Sproul-Jackson syndrome and acute myeloid leukemia (AML). We describe a patient presenting to the neuromuscular clinic with a de novo missense variant in DNMT3A where the striking clinical feature is that of a congenital myopathy with associated episodes of rhabdomyolysis, severe myalgias and chest pain along with phenotypic features associated with TBRS. Muscle biopsy showed minor myopathic features and cardiac investigations revealed mildly impaired bi-ventricular systolic function. We confirmed the DNA methylation profile matched haplo-insufficient TBRS cases, consistent with a loss of methyltransferase activity. Our report emphasizes the phenotypic overlap of patients with syndromic disorders presenting to neuromuscular clinics and limitations of gene panels in establishing a molecular diagnosis.
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Anormalidades Múltiplas , Deficiência Intelectual , Doenças Musculares , Rabdomiólise , Humanos , DNA (Citosina-5-)-Metiltransferases/genética , DNA Metiltransferase 3A , Mutação , Anormalidades Múltiplas/genética , Deficiência Intelectual/genética , Fenótipo , Rabdomiólise/diagnóstico , Rabdomiólise/genéticaRESUMO
Background: Myoadenylate deaminase (MAD) deficiency is a form of metabolic myopathy, which generally causes only mild symptoms in the primary inherited form. Inflammatory myopathies are a group of autoimmune diseases which result in skeletal muscle weakness. In addition to inflammatory pathology, it has been speculated that non-inflammatory mechanisms, and possibly secondary MAD-deficiency, may potentially contribute to weakness in these conditions. Methods: We investigated for an association between these two myopathic processes through two complementary methods. Firstly, muscle biopsy records in South Australia over a 17-year period were retrospectively reviewed for diagnosis of myositis or MAD-deficiency, as well as associated clinical features. Secondly, a prospective arm histochemically tested all incident biopsy specimens over a 12-month period for MAD-deficiency. Results: In the retrospective arm, 30 MAD-deficient cases were identified (1.3% of all biopsies), with no significant difference observed in overall rates of myositis diagnosis between patients with intact and deficient MAD activity (21.3% vs 26.7%, P = 0.47). No cases of MAD-deficiency were detected in the prospective arm, despite 39 cases of myositis being identified over this period. Conclusion: Secondary MAD deficiency is unlikely to be a major driver of symptoms in inflammatory myopathies.
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Immune-mediated necrotising myopathy (IMNM) is a recently described entity. We describe a cohort of South Australian IMNM patients in order to define the spectrum of disease, characterise features that distinguish IMNM from other idiopathic inflammatory myopathy (IIM) subtypes and identify factors associated with clinically severe disease. Subjects were identified from the South Australian Myositis Database (SAMD), a histologically defined registry. Consecutive muscle sections from patients with IMNM (nâ¯=â¯62), other forms of IIM (nâ¯=â¯60) and histologically normal muscle (nâ¯=â¯17) were stained using immunohistochemistry and graded. Clinical information was collected from the SAMD and through retrospective chart review. IMNM patients displayed clinical and histological heterogeneity. While most (67%) were profoundly weak at presentation, 24% exhibited mild to moderate weakness and 9% had normal power. Histological myonecrosis ranged from minor to florid. The amount of myofibre complement deposition was closely associated with clinical severity. Patients of Aboriginal and Torres Strait Islander heritage and those with anti-SRP autoantibodies present with a severe phenotype. Despite intense immunotherapy, few IMNM patients recovered full power at one year follow up. The identification of clinical, serological and histological features which are associated with severe forms of the disease may have diagnostic and therapeutic utility.
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Doenças Autoimunes , Miosite , Havaiano Nativo ou Outro Ilhéu do Pacífico/etnologia , Necrose , Sistema de Registros , Idoso , Autoanticorpos/sangue , Doenças Autoimunes/etnologia , Doenças Autoimunes/imunologia , Doenças Autoimunes/patologia , Doenças Autoimunes/fisiopatologia , Feminino , Seguimentos , Humanos , Imunoterapia , Masculino , Pessoa de Meia-Idade , Miosite/etnologia , Miosite/imunologia , Miosite/patologia , Miosite/fisiopatologia , Necrose/etnologia , Necrose/imunologia , Necrose/patologia , Necrose/fisiopatologia , Fenótipo , Estudos Retrospectivos , Índice de Gravidade de Doença , Austrália do Sul/etnologiaRESUMO
INTRODUCTION: High Mobility Group Box Protein 1 (HMGB1) is a DNA-binding protein that exerts inflammatory or pro-repair effects upon translocation from the nucleus. We postulate aberrant HMGB1 expression in immune-mediated necrotising myopathy (IMNM). METHODS: Herein, we compare HMGB1 expression (serological and sarcoplasmic) in patients with IMNM with that of other myositis subtypes using immunohistochemistry and ELISA. RESULTS: IMNM (n = 62) and inclusion body myositis (IBM, n = 14) patients had increased sarcoplasmic HMGB1 compared with other myositis patients (n = 46). Sarcoplasmic HMGB1 expression correlated with muscle weakness and histological myonecrosis, inflammation, regeneration and autophagy. Serum HMGB1 levels were elevated in patients with IMNM, dermatomyositis and polymositis, and those myositis patients with extramuscular inflammatory features. DISCUSSION: Aberrant HMGB1 expression occurs in myositis patients and correlates with weakness. A unique expression profile of elevated sarcoplasmic and serum HMGB1 was detected in IMNM.
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INTRODUCTION: Spinal laminectomy is a common procedure performed to relieve neural compression in patients suffering from myelopathy or radiculopathy. However, up to 40% of patients suffer from persistent post-operative pain and disability, a condition known as Failed Back Surgery Syndrome (FBSS). Excessive scarring in the surgical bed is implicated as a cause. Hydrogels have been proposed to prevent adhesion formation post-laminectomy; however, their efficacy has not been proven. This study uses Chitogel complexed with the iron chelator Deferiprone (Def) to prevent adhesion formation in a sheep laminectomy model. MATERIAL & METHODS: Fifteen Adult Merino sheep (Ovis Aries, 1-5 yrs old) underwent laminectomy at lumbar levels 1-5 and had hydrated aluminum silicate (kaolin) applied to promote adhesion formation. Subjects were randomised to receive at each laminectomy level no-treatment control, Chitogel, Chitogel with Def at 20 mM or 40 mM or Carboxy-methyl-cellulose and Polyethylene oxide (CMC/PEO) gel. The animals were recovered for 3 months post-surgery, followed by assessment with Magnetic Resonance Imaging (MRI) and histopathology of the spinal tissues for evaluating the presence and extent of adhesions. RESULTS: MRI and Histology assessment indicated that Kaolin induced severe inflammation with adhesion formation. Chitogel with and without 20 mM Def decreased inflammation (p < 0.01) and trended to reduce adhesions (p < 0.1). Chitogel with Def 40 mM was not significantly dis-similar to CMC/PEO and did not reduce inflammation or adhesions compared to no-treatment control. CONCLUSION: Chitogel in combination with Def 20 mM is safe and effective in decreasing the inflammatory process and may possibly reduce post-operative adhesions following laminectomy.
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Deferiprona/farmacologia , Laminectomia/efeitos adversos , Aderências Teciduais/prevenção & controle , Adulto , Animais , Cicatriz , Dura-Máter/patologia , Espaço Epidural/patologia , Síndrome Pós-Laminectomia , Feminino , Fibrose , Humanos , Vértebras Lombares/cirurgia , Masculino , Ovinos , Aderências Teciduais/patologiaRESUMO
The identification of high-grade glioma (HGG) progression may pose a diagnostic dilemma due to similar appearances of treatment-related changes (TRC) (e.g. pseudoprogression or radionecrosis). Deep learning (DL) may be able to assist with this task. MRI scans from consecutive patients with histologically confirmed HGG (grade 3 or 4) were reviewed. Scans for which recurrence or TRC was queried were followed up to determine whether the cases indicated recurrence/progression or TRC. Identified cases were randomly split into training and testing sets (80%/20%). Following development on the training set, classification experiments using convolutional neural networks (CNN) were then conducted using models based on each of diffusion weighted imaging (DWI - isotropic diffusion map), apparent diffusion coefficient (ADC), FLAIR and post-contrast T1 sequences. The sequence that achieved the highest accuracy on the test set was then used to develop DL models in which multiple sequences were combined. MRI scans from 55 patients were included in the study (70.1% progression/recurrence). 54.5% of the randomly allocated test set had progression/recurrence. Based upon DWI sequences the CNN achieved an accuracy of 0.73 (F1 scoreâ¯=â¯0.67). The model based on the DWI+FLAIR sequences in combination achieved an accuracy of 0.82 (F1 scoreâ¯=â¯0.86). The results of this study support similar studies that have shown that machine learning, in particular DL, may be useful in distinguishing progression/recurrence from TRC. Further studies examining the accuracy of DL models, including magnetic resonance perfusion (MRP) and magnetic resonance spectroscopy (MRS), with larger sample sizes may be beneficial.
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Neoplasias Encefálicas/diagnóstico por imagem , Aprendizado Profundo , Glioma/diagnóstico por imagem , Interpretação de Imagem Assistida por Computador/métodos , Imageamento por Ressonância Magnética/métodos , Recidiva Local de Neoplasia/diagnóstico por imagem , Adulto , Idoso , Neoplasias Encefálicas/patologia , Feminino , Glioma/patologia , Humanos , Masculino , Pessoa de Meia-Idade , Projetos PilotoRESUMO
The idiopathic inflammatory myopathies (IIMs) are a heterogeneous group of systemic muscle conditions that are believed to be autoimmune in nature. They have distinct pathological features, but the aetiopathogenesis of each subtype remains largely unknown. Recently, there has been increased interest in the complex role the innate immune system plays in initiating and perpetuating these conditions, and how this may differ between subtypes. This article summarises the traditional paradigms of IIM pathogenesis and reviews the accumulating evidence for disturbances in innate immune processes in these rare, but debilitating chronic conditions.
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Imunidade Inata , Miosite/imunologia , Animais , Quimiocinas/fisiologia , Regulação da Expressão Gênica/imunologia , Humanos , Sistema Imunitário/patologia , Macrófagos/imunologia , Músculo Esquelético/imunologia , Músculo Esquelético/metabolismo , Miosite/etiologiaAssuntos
Adenoma Pleomorfo , Neoplasias Oculares , Doenças do Aparelho Lacrimal , Aparelho Lacrimal , Adenoma Pleomorfo/diagnóstico , Adenoma Pleomorfo/patologia , Neoplasias Oculares/diagnóstico , Neoplasias Oculares/patologia , Humanos , Aparelho Lacrimal/diagnóstico por imagem , Aparelho Lacrimal/patologia , Doenças do Aparelho Lacrimal/diagnóstico , Doenças do Aparelho Lacrimal/patologiaRESUMO
Primitive neuroectodermal tumours (PNET) are highly malignant tumours with an aggressive clinical behaviour. Commonly seen in children, they are uncommon in the adult population, and rare in the supratentorial location. Adult supratentorial PNETs (ST-PNET) typically present with symptoms relating to raised intracranial pressure, seizures, or focal neurological deficits. Presentation with intracranial haemorrhage has been reported only twice before in the literature, one of which was fatal. We report the case of intracranial haemorrhage secondary to ST-PNET in a young adult and her immediate management.
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Hemorragias Intracranianas/etiologia , Tumores Neuroectodérmicos Primitivos/complicações , Neoplasias Supratentoriais/complicações , Adulto , Feminino , Humanos , Hemorragias Intracranianas/patologia , Tumores Neuroectodérmicos Primitivos/patologia , Neoplasias Supratentoriais/patologiaRESUMO
The clinical significance of curvilinear bodies (CB) seen in association with hydroxychloroquine (HCQ) therapy is uncertain. Patients with CB on muscle biopsy performed between 2006 and the present were identified, and their clinical features including body mass index and cumulative HCQ dose were recorded. A control group of 16 patients with idiopathic inflammatory myositis (IIM) on HCQ at time of biopsy but without evidence of CB was identified. Nineteen patients with CB were identified; details were available for 18. Among patients with CB, 7/18 also had IIM. Seven out of ten patients with CB who did not have IIM or MHCI/II expression had proximal weakness; 7/11 had raised serum creatinine kinase (CK) levels. There was no difference in body weight (p = 0.47), body mass index (p = 0.93), cumulative HCQ dose (p = 0.52) or cumulative dose adjusted for body weight (p = 0.39) or body mass index (p = 0.32) between patients with CB and controls. Patients with CB had lower median CK levels than controls (p = 0.034). Weakness was present in 12/17 patients and 12/16 controls (p = 1.0). Concurrent proton-pump inhibitors were co-prescribed in 12/18 (67 %) patients with CB and in 6/16 (38 %) controls (p = 0.17). Development of CB does not appear to be related to cumulative HCQ dose or body weight. Patients with CB frequently have muscle weakness in the absence of MHC1 expression suggesting a role for non-immune mechanisms of muscle injury. A high proportion of patients with CB are co-prescribed proton-pump inhibitors raising the possibility that co-prescription of both agents may disrupt lysosomal function and adversely affect muscle function.
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Antirreumáticos/efeitos adversos , Hidroxicloroquina/efeitos adversos , Debilidade Muscular/induzido quimicamente , Músculo Esquelético/patologia , Miosite/tratamento farmacológico , Antirreumáticos/uso terapêutico , Bases de Dados Factuais , Humanos , Hidroxicloroquina/uso terapêutico , Debilidade Muscular/patologia , Miosite/patologiaRESUMO
BACKGROUND: Bone marrow lesions (BMLs) are useful diagnostic and prognostic markers in knee osteoarthritis (OA), but what they represent at the tissue level remains unclear. The aim of this study was to provide comprehensive tissue characterization of BMLs detected using two specific MRI sequences. METHODS: Tibial plateaus were obtained from 60 patients (29 females, 31 males), undergoing knee arthroplasty for OA. To identify BMLs, MRI was performed ex vivo using T1 and PDFS-weighted sequences. Multi-modal tissue level analyses of the osteochondral unit (OCU) were performed, including cartilage volume measurement, OARSI grading, micro-CT analysis of bone microstructure, routine histopathological assessment and quantitation of bone turnover indices. RESULTS: BMLs were detected in 74 % of tibial plateaus, the remainder comprising a No BML group. Of all BMLs, 59 % were designated BML 1 (detected only by PDFS) and 41 % were designated BML 2 (detected by both PDFS + T1). The presence of a BML was related to degeneration of the OCU, particularly within BML 2. When compared to No BML, BML 2 showed reduced cartilage volume (p = 0.008), higher OARSI scores (p = 0.004), thicker subchondral plate (p = 0.002), increased trabecular bone volume and plate-like structure (p = 0.0004), increased osteoid volume (p = 0.002) and thickness (p = 0.003), more bone marrow oedema (p = 0.03), fibrosis (p = 0.002), necrosis (p = 0.01) and fibrovascular cysts (p = 0.04). For most measures, BML 1 was intermediate between No BML and BML 2. CONCLUSIONS: BMLs detected by specific MRI sequences identify different degrees of degeneration in the OCU. This suggests that MRI characteristics of BMLs may enable identification of different BML phenotypes and help target novel approaches to treatment and prevention of OA.