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The rajado seeded Andean bean (Phaseolus vulgaris L.) cultivar BRSMG Realce (striped seed coat) developed by Embrapa expressed a high level of anthracnose resistance, caused by Colletotrichum lindemuthianum, in field and greenhouse screenings. The main goal of this study was to evaluate the inheritance of anthracnose resistance in BRSMG Realce, map the resistance locus or major gene cluster previously named as Co-Realce, identify resistance-related positional genes, and analyze potential markers linked to the resistance allele. F2 plants derived from the cross BRSMG Realce × BRS FC104 (Mesoamerican) and from the cross BRSMG Realce × BRS Notável (Mesoamerican) were inoculated with the C. lindemuthianum races 475 and 81, respectively. The BRSMG Realce × BRS FC104 F2 population was also genotyped using the DArTseq technology. Crosses between BRSMG Realce and BAT 93 (Mesoamerican) were also conducted and resulting F2 plants were inoculated with the C. lindemuthianum races 65 and 1609, individually. The results shown that anthracnose resistance in BRSMG Realce is controlled by a single locus with complete dominance. A genetic map including 1,118 SNP markers was built and shown 78% of the markers mapped at a distances less than 5.0 cM, with a total genetic length of 4,473.4 cM. A major locus (Co-Realce) explaining 54.6% of the phenotypic variation of symptoms caused by the race 475 was identified in Pv04, flanked by the markers snp1327 and snp12782 and 4.48 cM apart each other. These SNPs are useful for marker-assisted selection, due to an estimated selection efficiency of 99.2%. The identified resistance allele segregates independently of the resistance allele Co-33 (Pv04) present in BAT 93. The mapped genomic region with 704,867 bp comprising 63 putative genes, 44 of which were related to the pathogen-host interaction. Based on all these results and evidence, anthracnose resistance in BRSMG Realce should be considered as monogenic, useful for breeding purpose. It is proposed that locus Co-Realce is unique and be provisionally designated as CoPv04R until be officially nominated in accordance with the rules established by the Bean Improvement Cooperative Genetics Committee.
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BACKGROUND: The introduction of biofortified foods such as beans with higher iron content may be a useful tool in preventing iron deficiency. The biofortification aims to reach the root of the problem of malnutrition, targets the neediest population, uses embedded distribution mechanisms, is scientifically feasible and effective in terms of cost, and complements other ongoing interventions to control micronutrient deficiency. However, to ensure effectiveness, measurement of the absorption of minerals is essential. OBJECTIVE: The objective of this study was to evaluate the iron bioavailability of common bean BRS Pontal (PO), targeted for biofortification, compared with common bean BRS Estilo in man through reliable techniques that have not been previously used in Brazil. METHODS: The study included 29 young adult volunteers divided into 2 groups: Group CB (13 subjects) received 100 g of common beans (BRS-Estilo) cooked labeled with iron-58 (58Fe) and Group TBB (16 patients) received 100 g common bean target for iron biofortification (BRS-Pontal), cooked and labeled with iron58 (58Fe). The next day they received the reference dose of ferrous sulfate enriched iron-57 (57Fe). Isotopic evaluation of iron for measurement of iron incorporation into erythrocytes was performed 14 days after consumption. The beans used, were produced, through conventional breeding program, by EMBRAPA/Rice and Beans. RESULTS: The iron absorption was evaluated by assessing the isotopic enrichment of the stable isotope. Mean iron absorption from the meal with common beans was 0.409% (±0.040%) and mean iron incorporation from the meal with target beans for biofortification 0.407% (±0.038%) and did not differ between the groups. CONCLUSIONS: This study tested the iron absorption from a single bean meal in healthy volunteers or non anemics, In the present study the iron absorption ratio from common bean Pontal (PO), targeted for biofortification and compared with common bean BRS Estilo was not significantly different. The iron concentration of the TBB, probably was not sufficient to improve higher bioavailability. It is recommended to evaluate the not only the absorption of iron in cultivars that present a higher difference in iron concentration but also in association with other components of the Brazilian diet, such as rice and beans.
Assuntos
Digestão , Alimentos Fortificados , Frutas/metabolismo , Absorção Intestinal , Ferro/metabolismo , Phaseolus/metabolismo , Plantas Geneticamente Modificadas/metabolismo , Adulto , Disponibilidade Biológica , Brasil , Culinária , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Phaseolus/genética , Phaseolus/crescimento & desenvolvimento , Plantas Geneticamente Modificadas/genética , Plantas Geneticamente Modificadas/crescimento & desenvolvimento , Adulto JovemRESUMO
The aim of this study was to identify and categorize new bean cultivars regarding their efficiency and responsiveness to nitrogen, phosphorus, and potassium, both individually and simultaneously, as well as to stipulate the cultivars that would suit each niche market based on this classification. Three experiments were conducted in the field separated by nutrients adopting the randomized block design in a factorial scheme (11 x 4) of 11 cultivars in four doses with three replications. Grain yield was the variable investigated and the means were the basis for the classification separated by in four availabilities: low, partial, adequate dose, and nutritional excess. Genetic variability was observed among the bean cultivars for efficiency and responsiveness (agronomic efficiency) to nitrogen, phosphorus, and potassium. The following cultivars: BRS Agreste, BRS Estilo, BRS Notável, and Pérola revealed the greatest values of efficiency and responsive ratings for nitrogen, phosphorus, and potassium, with indications for low and high technological levels. On the contrary, the cultivars BRS Ametista and BRS Sublime based on the efficiency and non-responsive classification showed a low ability to acquire or utilize the nutrients for grain formation. The BRS Pontal cultivar for all three nutrients displayed a high degree of efficiency with a value exceeding the average under different conditions of stress, suggesting that a more rustic cultivar best suits the market niche of small farmers.
O objetivo do trabalho foi identificar e classificar novas cultivares de feijoeiro quanto a eficiência e responsividade ao nitrogênio, fósforo e potássio, de forma individual e simultânea, além de indicar as cultivares para cada nicho de mercado, de acordo com essa classificação. Foram realizados três experimentos em campo, separados por nutriente, com delineamento de blocos casualizados, em esquema fatorial (11 x 4), sendo 11 cultivares em quatro doses, com três repetições. A variável analisada foi a produtividade de grãos e as médias utilizadas para a classificação, separando-se por nutriente, em quatro disponibilidades, sendo baixo, parcial, dose adequada e excesso nutricional. Existe variabilidade genética para eficiência e responsividade (eficiência agronômica) entre os cultivares de feijão, para nitrogênio, fósforo e potássio. As cultivares BRS Agreste, BRS Estilo, BRS Notável e a Pérola são as que possuem maior número de classificações eficientes e responsivas para nitrogênio, fósforo e potássio, sendo indicadas para baixo e alto nível tecnológico. Em sentido oposto, as cultivares BRS Ametista e a BRS Sublime com classificação ineficiente e não-responsiva, com baixa capacidade de adquirir ou utilizar os nutrientes para a formação de grãos. A cultivar BRS Pontal para nitrogênio, fósforo e potássio, apresenta uma eficiência alta ou acima da média sob os diferentes estresses, o que indica que é uma cultivar mais rústica e que atende o nicho de mercado de pequenos produtores.
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Fósforo , Potássio , Phaseolus , NitrogênioRESUMO
ABSTRACT: Using genotypes adapted to different regions is one of the main ways to increase Brazilian bean yield. The aim of the present study was to assess the genotypic performance of Carioca beans through mixed models. Fourteen Carioca bean genotypes were assessed in four locations in Pernambuco State (Arcoverde, Caruaru, Belém de São Francisco and São João counties) in 2015. The experiments followed a completely randomized block design, with three repetitions. Genetic parameters were estimated according to the REML/BLUP methodology, whereas genotype selection was based on the harmonic mean of relative performance of genetic values method (MHPRVG). The mean genotype heritability had moderate magnitude, high selective accuracy, besides allowing selection of agronomically superior individuals. Genotypes 'BRS Notável', CNFC 15480 and 'IPR 139' showed good adaptability and grain yield stability. There was agreement among the statistics μ ̂ + g ̂…, stability (MHVG), adaptability (PRVG), and stability and adaptability of genetic values (MHPRVG) in the discrimination of the most productive genotypes, which presented high adaptability and stability. This outcome indicated that these genotypes can be part of the selection criteria regularly used in bean breeding programs.
RESUMO: O uso de genótipos adaptados a diferentes regiões é uma das principais formas de aumentar a produtividade brasileira de feijão. O presente trabalho objetivou avaliar o desempenho genotípico de feijão carioca via modelos mistos. Foram avaliados 14 genótipos de feijão carioca em quatro locais do estado de Pernambuco (Arcoverde, Caruaru, Belém de São Francisco e São João) no ano de 2015. Os experimentos foram instalados em delineamento de blocos ao acaso com três repetições. Os parâmetros genéticos foram estimados pela metodologia REML/BLUP, e a seleção baseou-se no método da média harmônica da performance relativa dos valores genéticos (MHPRVG). A herdabilidade média dos genótipos apresentou magnitude moderada, aliada a alta acurácia seletiva, o que possibilita a seleção de indivíduos agronomicamente superiores. Os genótipos 'BRS Notável', CNFC 15480 e 'IPR 139' apresentaram boa adaptabilidade e estabilidade produtiva de grãos. Houve concordância entre as estatísticas μ ̂ + g ̂..., estabilidade de valores genéticos (MHVG), adaptabilidade de valores genéticos (PRVG) e estabilidade e adaptabilidade de valores genéticos (MHPRVG) na discriminação dos genótipos mais produtivos e com alta adaptabilidade e estabilidade, indicando que elas podem fazer parte de critérios seletivos na rotina dos programas de melhoramento do feijão.
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ABSTRACT:More efficient strains of Rhizobiumhave been selected for use in common bean. However, little effort was made with lines selection. The main goals of this research were to verify the presence of interaction involving common bean elite lines utilizing Nitrogen fertilization and Rhizobiuminoculation for grain yield and to identify lines with superior yields utilizing biological nitrogen fixation. Eight field trials were conducted at four location-years in Brazilian savanna, using randomized complete blocks design with three replications. Each trial was composed of 17 carioca elite lines. Every two tests in each location were planted side by side, one with mineral nitrogen fertilization (90kg) and the other one with inoculation with Rhizobium tropiciSEMIA 4080 strain. Elite lines interaction with nitrogen fertilization/inoculation was not important, so, it is possible to select lines for utilization in both growing systems. In some locations-years, interaction between lines and Rhizobiuminoculation was most affected by environment conditions, causing modification in lines classification according to the type of nitrogen supplying used. In general, the lines presented higher yields when fertilized with mineral nitrogen as compared with inoculation. The cultivar 'BRS Pontal' presented high and similar yields under both systems of nitrogen supply.
RESUMO:Estirpes de rizóbio mais eficientes têm sido selecionadas para uso em feijoeiro-comum. Entretanto, pouco tem sido feito na seleção de linhagens mais eficientes. Os objetivos desse trabalho foram verificar a presença de interação entre linhagens elite de feijoeiro-comum com uso de fertilização nitrogenada e inoculação com rizóbio, para produtividade de grãos e identificar linhagens com produção superior, quando inoculadas com rizóbio. Oito experimentos foram conduzidos em quatro locais/anos no cerrado brasileiro, compostos por 17 linhagens elite, em blocos ao acaso com três repetições. Em cada local/ano foram conduzidos dois experimentos, lado a lado: um com fertilização mineral de nitrogênio (90kg) e outro com inoculação com rizóbio. A interação entre as linhagens elite e o tipo de fornecimento de nitrogênio não foi importante, indicando que é possível selecionar linhagens para uso simultâneo nos dois sistemas de cultivo. Em alguns locais, a interação entre as linhagens e a inoculação com rizóbio foi mais afetada pelo ambiente, causando modificações na classificação das linhagens nos dois tipos de fornecimento de nitrogênio. De modo geral, a produtividade foi maior utilizando-se fertilização mineral, quando comparada com a inoculação. A cultivar 'BRS Pontal' apresentou produtividade alta e semelhante nos dois sistemas de fornecimento de nitrogênio.
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Objetivou-se, neste trabalho, identificar linhagens de feijão que reúnam, além da resistência à antracnose, alta produtividade de grãos do tipo carioca e resistência à mancha angular. Foram utilizadas 194 linhagens F5:6 extraídas de sete famílias segregantes, selecionadas do cruzamento entre os genitores H147 e B1. A linhagem H147 possui grãos tipo carioca, portadora do alelo Co-5, que confere resistência a várias raças de Colletotrichum lindemuthianum. A linhagem B1 também possui grãos tipo carioca e é portadora do alelo Co-4, que confere resistência a outro grupo de raças do mesmo patógeno. As linhagens foram avaliadas na safra das águas 2005/2006, em Lavras, com a cultivar Talismã e H147 como testemunhas, com base na produtividade e tipo de grãos. Foram selecionadas 99 linhagens, as quais foram avaliadas na safra da seca/2006, juntamente com a testemunha Talismã, com base na produtividade, tipo de grão e resistência à mancha angular. Dessas 99 linhagens, foram selecionadas 24, as quais foram avaliadas na safra de inverno/2006 em Lavras e Lambari, com base no tipo de grão e produtividade. Essas 24 linhagens foram inoculadas com a raça 321 de C. lindemuthianum, que quebra a resistência conferida pelo alelo Co-4, mas não o Co-5. Para verificar a presença do alelo Co4 foi utilizado um marcador SCAR que amplifica um fragmento de 950 pb por meio do primer SAS 13. Foi possível identificar 14 linhagens que possuem a pirâmide de alelos Co-4/Co-5 e entre elas, quatro destacaram-se em todos os caracteres avaliados.
The objective of the research was to identify bean strains that possess at the same time resistance to anthracnose, high grain yield of Carioca grain type and resistance to angular leaf spot. 194 strains F5:6 were taken from seven segregating families derived from the cross H147 x B1. The H147 line has Carioca grain type and Co-5 resistance allele to several races of C. lindemuthianum. The B1 line also has the Carioca grain type, and the Co-4 resistance allele against others races of the same pathogen. The strains were evaluated in the spring/summer (2005/2006) in the municipality of Lavras, in the Southern of the State of Minas Gerais, with the Talismã cultivar and H147 line as check, based mainly on grain type and grain yield. 99 strains, together with the Talismã check, were selected and evaluated based on grain yield, grain type and angular leaf spot resistance in the summer (2006) in the same place. From these 99 strains, 24 strains were selected and evaluated in the winter/spring (2006) in two places, Lavras and Lambari, based on the type of grain and productivity, and using the same check. Those 24 strains were also inoculated with the race 321 of C. lindemuthianum that break the resistance of the Co-4 allele, but not of the Co-5. The SCAR marker using the SAS13 primer was used to detect the presence of the Co-4 allele. Fourteen strains were identified that have the Co-4/Co-5 allele pyramid, and, among them, four stand out in all evaluated features.
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Objetivou-se neste trabalho, selecionar famílias de feijoeiro promissoras para a produtividade de grãos com tipo de grãos ideal utilizando informações fenotípicas e de marcadores moleculares ligados a QTLs. Foram utilizadas 100 famílias F3:7, avaliadas em três safras, com dois experimentos/safra, totalizando seis experimentos. A primeira safra foi a da seca/2007, na qual conduziu-se um experimento em Lavras-MG e o outro em Ijaci-MG. Nas safras de inverno/2007 e águas 2007/2008, foram conduzidos, em cada uma, um experimento em Lavras-MG e outro em Lambari-MG. Em todos eles foi utilizado o delineamento látice triplo 10x10, com parcelas de duas linhas de dois metros. As famílias foram avaliadas pela sua produtividade de grãos. Em apenas um dos experimentos de cada safra foi avaliado o tipo de grão. Os dados foram submetidos à análise de variância individual e conjunta, por local e por safra. 480 marcadores microssatélites foram testados para identificar polimorfismo entre os genitores. Os oito marcadores polimórficos identificados foram utilizados para a genotipagem das famílias. Entre esses, cinco explicaram parte da variação da produtividade de grãos. Os marcadores explicaram pequena porcentagem da variação fenotípica e apresentaram alta interação QTLs x ambientes. O ganho com a seleção fenotípica para produtividade de grãos foi de 7,4 por cento, e de 9,6 por cento para tipo de grãos, adotando a intensidade de seleção de 5 por cento. A seleção assistida por marcadores foi equivalente à fenotípica para produtividade de grãos, porque apenas um marcador mais estável contribuiu para a seleção com base na média dos ambientes.
The objectives of this research were to select com mon bean families with high grain yield and ideal grain type using phenotypical information and molecular markers linked to the QTLs. One hundred F3:7 families were evaluated in three seasons, in two field experiments per season. Two experiments were set up in the counties of Lavras and Ijaci in the dry season/2007, other four experiments were set up in Lavras and Lambari, two in the winter season/2007 and two in the 2007/2008 rainy season. A 10x10 lattice design was used in all experiments, using 2m-long plots and rows 50cm apart. The families were evaluated based on grain yield in all experiments, and based on grain type in one experiment per season. Moreover, 480 microsatellite markers were tested in the parents. Eight markers were polymorphic and used for genotyping the families. Among them five explained part of the grain yield variability and presented high interaction by environments. Selecting 5 percent of the families the genetic gain was of 7,4 percent for grain yield and 9,6 percent for grain type. The marker-assisted selection gave a similar result to the phenotypic selection because only one more stable marker of the along with average grain yield was used in the selection.