RESUMO
Fucoidans constitute a large family of sulfated polysaccharides with several biochemical properties. A commercial fucoidan from brown algae, containing low molecular weight polysaccharidic species constituted of l-fucose, uronic acids and sulfate groups, was simply treated here with calcium acetate solution. This treatment led to a purified fraction with a yield of 45%. The physicochemical characterizations of the purified fucoidan using colorimetric assay, MALLS, dRI, FT-IR, NMR, exhibited molecular weight distributions and chemical profiles similar for both fucoidans whereas the sulfate and l-fucose contents increased by 16% and 71%, respectively. The biodistribution study in rat of both compounds labeled with 99mTc evidenced a predominant renal elimination of the purified fucoidan, but the crude fucoidan was mainly retained in liver and spleen. In rat myocardial ischemia-reperfusion, we then demonstrated the better efficiency of the purified fucoidan. This purified sulfated polysaccharide appears promising for the development of molecular imaging in acute coronary syndrome.
Assuntos
Infarto do Miocárdio/diagnóstico por imagem , Polissacarídeos/isolamento & purificação , Compostos Radiofarmacêuticos/isolamento & purificação , Tomografia Computadorizada de Emissão de Fóton Único/métodos , Animais , Fucose/análise , Ácido Glucurônico/análise , Marcação por Isótopo , Masculino , Peso Molecular , Traumatismo por Reperfusão Miocárdica/diagnóstico por imagem , Polissacarídeos/química , Polissacarídeos/farmacocinética , Compostos Radiofarmacêuticos/farmacocinética , Ratos , Ratos Wistar , Tecnécio , Distribuição TecidualRESUMO
Novel 2,3-diaminopropionic acid-based molecules were synthesised and tested successfully as glyoxal/methylglyoxal scavengers and as AGE inhibitors. Addition of an 8-hydroxyquinoline moiety led to an increase of the overall activity. The compounds tested in this study were also proved to be efficient in trapping ALE precursor malondialdehyde.